##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049540_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55376546
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44555032377787	31.0	31.0	34.0	30.0	34.0
2	31.679381700693288	33.0	31.0	34.0	30.0	34.0
3	31.76980709486648	34.0	31.0	34.0	30.0	34.0
4	35.29709536235792	37.0	35.0	37.0	33.0	37.0
5	35.27088666743498	37.0	35.0	37.0	33.0	37.0
6	35.253225273385596	37.0	35.0	37.0	33.0	37.0
7	35.25497106663171	37.0	35.0	37.0	33.0	37.0
8	35.25662739962149	37.0	35.0	37.0	33.0	37.0
9	36.87763830557435	39.0	37.0	39.0	33.0	39.0
10-11	36.86567275250428	39.0	37.0	39.0	33.0	39.0
12-13	36.838299945612356	39.0	37.0	39.0	33.0	39.0
14-15	38.12886238878099	40.0	38.0	41.0	33.0	41.0
16-17	38.07160524240714	40.0	38.0	41.0	33.0	41.0
18-19	38.11152027249948	40.0	38.0	41.0	33.5	41.0
20-21	38.101254057990545	40.0	38.0	41.0	33.5	41.0
22-23	38.033683818055394	40.0	38.0	41.0	33.0	41.0
24-25	37.98925481917922	40.0	38.0	41.0	33.0	41.0
26-27	37.91711945161766	40.0	38.0	41.0	33.0	41.0
28-29	37.83820694595145	40.0	38.0	41.0	33.0	41.0
30-31	37.77035333695244	40.0	38.0	41.0	33.0	41.0
32-33	37.701170193966234	40.0	38.0	41.0	33.0	41.0
34-35	37.61564303775826	40.0	38.0	41.0	32.5	41.0
36-37	37.52936587991602	40.0	38.0	41.0	32.0	41.0
38-39	37.430661267678204	40.0	37.5	41.0	32.0	41.0
40-41	37.360078281155346	40.0	37.0	41.0	32.0	41.0
42-43	37.22556256036626	40.0	37.0	41.0	32.0	41.0
44-45	37.11137626749057	40.0	37.0	41.0	31.0	41.0
46-47	36.9767570281469	40.0	36.5	41.0	31.0	41.0
48-49	36.852276575718534	40.0	36.0	41.0	31.0	41.0
50-51	36.710463415323886	40.0	36.0	41.0	31.0	41.0
52-53	36.54584112197969	39.5	35.0	41.0	30.5	41.0
54-55	36.37352808172615	39.0	35.0	41.0	30.0	41.0
56-57	36.17557722903122	39.0	35.0	41.0	30.0	41.0
58-59	35.977853024636104	39.0	35.0	41.0	30.0	41.0
60-61	35.72349427499505	38.0	35.0	40.5	29.0	41.0
62-63	35.48886012139508	38.0	35.0	40.0	29.0	41.0
64-65	35.22129149225017	37.0	35.0	40.0	29.0	41.0
66-67	34.953830101646275	37.0	34.0	40.0	29.0	41.0
68-69	34.65579714921187	36.0	34.0	39.5	28.0	41.0
70-71	34.325029833027145	36.0	34.0	39.0	28.0	41.0
72-73	34.02542348885393	35.0	34.0	39.0	28.0	40.5
74-75	33.71555087599721	35.0	34.0	38.0	28.0	40.0
76-77	33.37915885003012	35.0	34.0	37.0	27.0	39.0
78-79	33.09922386636393	35.0	33.0	37.0	27.0	39.0
80-81	32.813424360197544	35.0	33.0	36.0	27.0	39.0
82-83	32.557192308454916	35.0	33.0	36.0	26.0	37.5
84-85	32.30783062923426	35.0	33.0	35.5	26.0	37.0
86-87	32.07557200299202	35.0	33.0	35.0	26.0	37.0
88-89	31.92091863223105	35.0	33.0	35.0	26.0	36.0
90-91	31.714108126209247	35.0	33.0	35.0	25.0	36.0
92-93	31.555768032191825	35.0	33.0	35.0	25.0	36.0
94-95	31.393388583679453	35.0	33.0	35.0	24.5	35.5
96-97	31.257308473157572	35.0	33.0	35.0	24.0	35.0
98-99	31.0753234410828	35.0	33.0	35.0	23.5	35.0
100	30.956985255093375	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17155765864607275
1101	2	0.2228942416543589
1101	3	0.23817245807984122
1101	4	0.2527691526176241
1101	5	0.23301218019058467
1101	6	0.15684822482217697
1101	7	0.14257057585510324
1101	8	0.1910294370187131
1101	9	0.270300441745313
1101	10-11	0.27330192330609293
1101	12-13	0.24124973379053216
1101	14-15	0.32469515050016184
1101	16-17	0.3683038937325449
1101	18-19	0.35875651102136885
1101	20-21	0.33365621016867664
1101	22-23	0.31107415566106056
1101	24-25	0.3629989691173279
1101	26-27	0.33382412892006386
1101	28-29	0.35046830365424597
1101	30-31	0.3416350571921285
1101	32-33	0.29608136263480844
1101	34-35	0.30820971561506383
1101	36-37	0.32966595242849905
1101	38-39	0.3611423514030605
1101	40-41	0.3478500347417892
1101	42-43	0.31565769077514005
1101	44-45	0.3259875098584075
1101	46-47	0.33539083808785364
1101	48-49	0.32156384284657236
1101	50-51	0.35377630927695947
1101	52-53	0.3527162046703438
1101	54-55	0.3392690690933051
1101	56-57	0.3903458424565258
1101	58-59	0.3556588126400655
1101	60-61	0.3303571731436463
1101	62-63	0.3219579660805252
1101	64-65	0.34186284282117896
1101	66-67	0.3632942775797545
1101	68-69	0.36676507124016666
1101	70-71	0.3606741452231361
1101	72-73	0.3916716185059812
1101	74-75	0.37917608680211856
1101	76-77	0.36781186780137887
1101	78-79	0.33731883302446874
1101	80-81	0.30111072595915545
1101	82-83	0.3506278452412346
1101	84-85	0.35630048785809265
1101	86-87	0.40531809619341175
1101	88-89	0.36105246037041283
1101	90-91	0.36545955013912135
1101	92-93	0.3612722907415069
1101	94-95	0.32387004387901897
1101	96-97	0.3370314421183167
1101	98-99	0.3461653639846425
1101	100	0.39863201763984435
1102	1	0.17747756447687024
1102	2	0.24244003439830308
1102	3	0.2055129938191662
1102	4	0.205500670455244
1102	5	0.18855750420438966
1102	6	0.15191540400054038
1102	7	0.15251796587116218
1102	8	0.12418040072382297
1102	9	0.14096975794854671
1102	10-11	0.1618508809123398
1102	12-13	0.1350661920895675
1102	14-15	0.22700833357133376
1102	16-17	0.28110147168279553
1102	18-19	0.23958828857860226
1102	20-21	0.2246271229550061
1102	22-23	0.18938833417472978
1102	24-25	0.15878338884334653
1102	26-27	0.09986130412796257
1102	28-29	0.22898457558402185
1102	30-31	0.2332594081191175
1102	32-33	0.14619768231989383
1102	34-35	0.23191110569186435
1102	36-37	0.21578090347197332
1102	38-39	0.23253656091886654
1102	40-41	0.1899415829707678
1102	42-43	0.2031201510320244
1102	44-45	0.16525542988794228
1102	46-47	0.17581456885783808
1102	48-49	0.1517155353746915
1102	50-51	0.19021032230591572
1102	52-53	0.144943422687831
1102	54-55	0.14791385184837935
1102	56-57	0.21358487089231204
1102	58-59	0.1989553969234592
1102	60-61	0.17435317315536736
1102	62-63	0.1822899635157711
1102	64-65	0.16421848451027898
1102	66-67	0.175578088316243
1102	68-69	0.188535996374938
1102	70-71	0.2498058577173552
1102	72-73	0.23534506533266608
1102	74-75	0.22148775382388664
1102	76-77	0.18647005973329556
1102	78-79	0.17001808781022731
1102	80-81	0.17841886896417947
1102	82-83	0.1936794650827025
1102	84-85	0.19038138013041817
1102	86-87	0.18341061212301213
1102	88-89	0.1418823228125241
1102	90-91	0.17049201615144582
1102	92-93	0.19732796442997724
1102	94-95	0.1957707115011651
1102	96-97	0.12195843403844009
1102	98-99	0.19994812517110105
1102	100	0.2323568570842518
1103	1	0.15189088970379672
1103	2	0.1758829368434185
1103	3	0.139893340015238
1103	4	0.1048915651964819
1103	5	0.10054478349841389
1103	6	0.05029913397859076
1103	7	0.03156339231102123
1103	8	-0.010524088058360803
1103	9	0.08965145987009748
1103	10-11	0.057700738997091605
1103	12-13	-0.023035567987150785
1103	14-15	0.03552495298326619
1103	16-17	0.1361843948381818
1103	18-19	0.07121280500967231
1103	20-21	0.048093744481228384
1103	22-23	0.06579499598962002
1103	24-25	0.038762093941890896
1103	26-27	-0.0062190134755937265
1103	28-29	0.023935029720291823
1103	30-31	0.04675609864701613
1103	32-33	0.03991892531613672
1103	34-35	0.05975895884068905
1103	36-37	0.040769466443492774
1103	38-39	0.046444593979060755
1103	40-41	0.07000448619069033
1103	42-43	0.08210826347735178
1103	44-45	0.018416972668184428
1103	46-47	0.026663685713621987
1103	48-49	0.08082996700537137
1103	50-51	0.06265958384933157
1103	52-53	0.04034270229612957
1103	54-55	0.03161617711484155
1103	56-57	0.06176991535945575
1103	58-59	0.022168061143354123
1103	60-61	0.048085556045151634
1103	62-63	0.030497748851630035
1103	64-65	0.017033928234752693
1103	66-67	0.020828224070456258
1103	68-69	0.08085019730403076
1103	70-71	0.07414729392525743
1103	72-73	0.05111650882680152
1103	74-75	0.06527116789530396
1103	76-77	0.08311094953447906
1103	78-79	0.006136337331028585
1103	80-81	0.005212906563492936
1103	82-83	0.03277612429786814
1103	84-85	-0.023118102368549387
1103	86-87	-0.0196305623197901
1103	88-89	-0.013996811147340082
1103	90-91	0.013340441274706905
1103	92-93	0.027867554895326663
1103	94-95	0.03265725017831045
1103	96-97	0.03290781410000676
1103	98-99	0.055144105795310594
1103	100	0.09175328698217555
1104	1	0.040105887777851734
1104	2	0.018874419542321164
1104	3	0.0526614141649695
1104	4	0.059708528831087904
1104	5	0.022644792636711486
1104	6	0.019489726865593582
1104	7	0.005906010580169152
1104	8	-0.06214670988231319
1104	9	-0.08779478024695209
1104	10-11	-0.010460420397194525
1104	12-13	-0.009920912047775232
1104	14-15	0.030006607646910766
1104	16-17	0.022128443306719703
1104	18-19	-0.0601642911629412
1104	20-21	-0.04866245958483262
1104	22-23	-0.12020653273568627
1104	24-25	-0.0807397555884819
1104	26-27	-0.10781500815078715
1104	28-29	-0.1365190942831518
1104	30-31	-0.1174639968912814
1104	32-33	-0.08619349901429985
1104	34-35	-0.10967120837217692
1104	36-37	-0.0861745527022677
1104	38-39	-0.04814504731157143
1104	40-41	-0.09332251245338341
1104	42-43	-0.1261453843232232
1104	44-45	-0.09379383755621262
1104	46-47	-0.10642367857976609
1104	48-49	-0.12694888048315534
1104	50-51	-0.15461437621871
1104	52-53	-0.05370683824180844
1104	54-55	-0.0847271769239768
1104	56-57	-0.07566120308212732
1104	58-59	-0.08514499995784774
1104	60-61	-0.06794573450830654
1104	62-63	-0.06047596334598637
1104	64-65	-0.12338222635448659
1104	66-67	-0.1328211890400155
1104	68-69	-0.03296709660133956
1104	70-71	-0.1362182288545668
1104	72-73	-0.10768429402160251
1104	74-75	-0.1349389830014971
1104	76-77	-0.14264049312967586
1104	78-79	-0.1802954737681688
1104	80-81	-0.12616182041701762
1104	82-83	-0.12066277568772676
1104	84-85	-0.13781371254908237
1104	86-87	-0.10086082217796744
1104	88-89	-0.1939275803957905
1104	90-91	-0.15121034069382944
1104	92-93	-0.17032703628678902
1104	94-95	-0.14322256472526007
1104	96-97	-0.13966149190127553
1104	98-99	-0.10839900109569456
1104	100	-0.06662978879492343
1105	1	0.029488746179808345
1105	2	0.012897751891209452
1105	3	0.041876164726161846
1105	4	0.040016675867093454
1105	5	0.03156765589935162
1105	6	-0.03479630207444018
1105	7	-0.018860407740852736
1105	8	0.035244249064760425
1105	9	0.06059230832742912
1105	10-11	-0.006676866519981672
1105	12-13	-0.009672416177394894
1105	14-15	0.0272180653069114
1105	16-17	0.04540932774946782
1105	18-19	-0.018085296432502673
1105	20-21	-0.016972687275632836
1105	22-23	-0.07219449129436839
1105	24-25	2.196579650615149E-5
1105	26-27	-0.07304728364826474
1105	28-29	-0.06652574335232231
1105	30-31	-0.047210432888512344
1105	32-33	-0.028823122650457833
1105	34-35	0.021522773737707723
1105	36-37	0.00946684725973057
1105	38-39	-0.012078956726405465
1105	40-41	0.004243081445714836
1105	42-43	-0.15131459902995914
1105	44-45	-0.04995670017920162
1105	46-47	-0.1237566232189593
1105	48-49	-0.08971796353064576
1105	50-51	-0.15844810294363043
1105	52-53	-0.08052555918467874
1105	54-55	-0.03743691702945284
1105	56-57	-0.10509898002992912
1105	58-59	-0.0647976514492612
1105	60-61	-0.047257163017739856
1105	62-63	-0.05380271347154775
1105	64-65	-0.09615346194649987
1105	66-67	-0.02796186703197634
1105	68-69	-0.019593800476854994
1105	70-71	0.0350403243333659
1105	72-73	0.005229706453974359
1105	74-75	-0.03023060019460644
1105	76-77	-2.2279967248550747E-4
1105	78-79	-0.0706331675545897
1105	80-81	-0.03625345843912697
1105	82-83	-0.03205893496043899
1105	84-85	-0.059877215452480925
1105	86-87	-0.05788580615218564
1105	88-89	-0.09619743417059823
1105	90-91	-0.06998987102003795
1105	92-93	-0.14190954787985532
1105	94-95	-0.0882226477750443
1105	96-97	-0.051073820503980016
1105	98-99	0.001715864445660742
1105	100	-0.03135320547557896
1106	1	0.04383737663297893
1106	2	0.02246565122013422
1106	3	0.006777829704347482
1106	4	0.046932822391219986
1106	5	0.032724939607334136
1106	6	-0.019680468834010867
1106	7	-0.021608528163540086
1106	8	-0.033364622904556995
1106	9	-0.016542036821647343
1106	10-11	-0.03788685057496366
1106	12-13	-0.055691676704597626
1106	14-15	-0.08263129730099195
1106	16-17	-0.048355585387277245
1106	18-19	-0.03551533835754839
1106	20-21	-0.06647663776289647
1106	22-23	-0.02471833480854002
1106	24-25	-0.008269879195310637
1106	26-27	-0.07069438254377047
1106	28-29	-0.06102818381135933
1106	30-31	-0.08002971432802042
1106	32-33	-0.05980530410946017
1106	34-35	-0.01938103517455403
1106	36-37	-0.01687116334623795
1106	38-39	-0.014503041539605022
1106	40-41	-0.0632867039108973
1106	42-43	-0.08479552375519717
1106	44-45	-0.06680508454245881
1106	46-47	-0.1064148987154212
1106	48-49	-0.07042119057246055
1106	50-51	-0.03676609651076035
1106	52-53	-0.04952438910757451
1106	54-55	-0.10783340627370563
1106	56-57	-0.10523191965705792
1106	58-59	-0.06149330991741664
1106	60-61	-0.05214632936276331
1106	62-63	-0.034931114290778
1106	64-65	-0.026106121785090863
1106	66-67	-0.054570090084837375
1106	68-69	-0.08628853759727662
1106	70-71	-0.07255220645049576
1106	72-73	-0.06735620915790008
1106	74-75	-0.07478798197978165
1106	76-77	-0.07597424003435549
1106	78-79	-0.13331512691766534
1106	80-81	-0.09046051256689935
1106	82-83	-0.10638871836228248
1106	84-85	-0.12857277477717588
1106	86-87	-0.08162812318663626
1106	88-89	-0.10526011655271716
1106	90-91	-0.08093160928210708
1106	92-93	-0.08001898334276447
1106	94-95	-0.040046867234810435
1106	96-97	-0.04660029345967587
1106	98-99	-0.022746046733743697
1106	100	0.0025819674015927774
1107	1	-0.01765968451452693
1107	2	-0.011904952297630444
1107	3	-0.02895821207050986
1107	4	-0.004899825431579075
1107	5	-2.198641026183168E-4
1107	6	-0.05183779780377762
1107	7	-0.03053953944446164
1107	8	-0.07334244448441751
1107	9	-0.018097090146007133
1107	10-11	-0.046079595742504864
1107	12-13	-0.03635772289685235
1107	14-15	-0.0967381206633604
1107	16-17	-0.058410368155165315
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2216	60-61	-0.3600599982779258
2216	62-63	-0.37547080383428266
2216	64-65	-0.5342851429196003
2216	66-67	-0.4972988915038883
2216	68-69	-0.3721534202237393
2216	70-71	-0.44371863141959267
2216	72-73	-0.405201062110109
2216	74-75	-0.388828440449835
2216	76-77	-0.36210934540532946
2216	78-79	-0.4524301605786434
2216	80-81	-0.41415063325099766
2216	82-83	-0.4356773568627119
2216	84-85	-0.4834640855783263
2216	86-87	-0.47652764784701773
2216	88-89	-0.5178359891507931
2216	90-91	-0.48561422707228985
2216	92-93	-0.4722788424459914
2216	94-95	-0.5621070496922265
2216	96-97	-0.4948129419129792
2216	98-99	-0.4823866892454909
2216	100	-0.5131160679444058
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	564335.0
3	134694.0
4	74206.0
5	67138.0
6	71019.0
7	74041.0
8	75121.0
9	82343.0
10	93555.0
11	99400.0
12	102603.0
13	107805.0
14	110835.0
15	113817.0
16	116563.0
17	118756.0
18	122279.0
19	125046.0
20	130987.0
21	140319.0
22	154596.0
23	180082.0
24	218175.0
25	269284.0
26	337276.0
27	424389.0
28	529594.0
29	657162.0
30	820356.0
31	1046960.0
32	1358935.0
33	1812225.0
34	2577666.0
35	3991773.0
36	6926895.0
37	1.1887744E7
38	1.4822412E7
39	4819309.0
40	16851.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.004102544385233	29.294339882969762	22.87911856245385	25.822439010191157
2	21.843234203328127	28.449652411136935	26.503854567280445	23.203258818254493
3	23.078835266846195	26.521555029750566	25.145009846421303	25.25459985698194
4	23.04173015392232	25.70455455846108	25.479271763379984	25.774443524236613
5	22.998613809134625	26.369418898104946	26.34102631798673	24.290940974773697
6	23.632565520969965	25.873098093371144	25.59699212134533	24.89734426431356
7	23.787104038707934	25.902468176421095	26.155879204079703	24.15454858079127
8	22.76263153610533	26.438786139658998	26.715397234258393	24.08318508997727
9	22.91839721336624	26.076082274762037	26.70971133376543	24.295809178106293
10-11	23.081361589309346	26.287459408274334	26.638392476729656	23.99278652568667
12-13	23.1179380338015	26.109541528762342	26.740673705740104	24.03184673169605
14-15	23.218337045434364	26.057451736839287	26.772151413378083	23.952059804348263
16-17	23.336506181930794	25.957735150824586	26.688784598675515	24.01697406856911
18-19	23.40749138188975	25.79803452878521	26.61999180691551	24.17448228240953
20-21	23.4562704435363	25.771420046020392	26.60113780832164	24.17117170212167
22-23	23.558166353307257	25.797221699969114	26.4931480517625	24.151463894961132
24-25	23.66731951090512	25.7232202848577	26.355767742054166	24.253692462183015
26-27	23.700488853677413	25.75036222828419	26.34225176116969	24.20689715686871
28-29	23.74552171389469	25.78498993194303	26.256711445459334	24.21277690870295
30-31	23.832101810776006	25.677454552344958	26.205234794814338	24.2852088420647
32-33	23.857393497065733	25.70959206145247	26.180682180046116	24.252332261435686
34-35	23.87689382598045	25.74585886304415	26.123706083870985	24.253541227104417
36-37	23.921256050748084	25.657131577150018	26.080921886212582	24.34069048588931
38-39	23.916405818499655	25.658437648379127	26.11352817640549	24.31162835671572
40-41	23.960223335896842	25.67739465153725	26.066545608708235	24.295836403857674
42-43	24.010829544256257	25.57901515753369	26.006768899779072	24.403386398430982
44-45	23.99482155956895	25.590217013964505	26.050709967837715	24.36425145862883
46-47	24.0491927755372	25.631971047534353	25.975515677621075	24.34332049930737
48-49	24.06842776535734	25.540572773115006	25.94566460208948	24.445334859438177
50-51	24.041505260705424	25.590194690923614	25.970316930817262	24.397983117553697
52-53	24.06571606634598	25.594623320844008	25.949483398864697	24.390177213945318
54-55	24.120898625488874	25.549410849538162	25.88329731388514	24.44639321108783
56-57	24.1232250849305	25.566458335150198	25.91483700443066	24.39547957548864
58-59	24.12792054722221	25.613115950290073	25.884818643593626	24.37414485889409
60-61	24.164034216346387	25.544937344061342	25.81803464078182	24.47299379881045
62-63	24.14826646789818	25.561100458077284	25.85255989273993	24.438073181284604
64-65	24.188435548166545	25.61242567497899	25.810824977110475	24.388313799743997
66-67	24.237957076966303	25.560990510815884	25.755808880109793	24.44524353210802
68-69	24.19563036947561	25.586930113589247	25.78507036157278	24.432369155362363
70-71	24.255979565483045	25.62015691501393	25.735158129273373	24.388705390229656
72-73	24.28611743648048	25.569561264845902	25.697054557370368	24.44726674130325
74-75	24.282046591539775	25.591026794704767	25.734945453430036	24.391981160325415
76-77	24.310381354665783	25.643210609720494	25.68535650728855	24.361051528325177
78-79	24.334132089231787	25.594983822496648	25.653519670487313	24.417364417784253
80-81	24.324892304083548	25.620042738738967	25.678340045129715	24.376724912047766
82-83	24.32611567706215	25.6656087134225	25.65072946278025	24.3575461467351
84-85	24.355952425519707	25.62249641900276	25.600262746107877	24.421288409369655
86-87	24.33970113250274	25.658418981804093	25.61734318503938	24.384536700653786
88-89	24.37815358212453	25.668913526770687	25.592784734812106	24.360148156292677
90-91	24.415286468771654	25.635130023090664	25.536424225761227	24.413159282376455
92-93	24.402657882385537	25.663516935638242	25.562268594627565	24.371556587348657
94-95	24.434865206563455	25.72048827667889	25.511944411101023	24.332702105656633
96-97	24.471730835947355	25.710996395629532	25.454021367563247	24.36325140085987
98-99	24.49025139471974	25.754203040831207	25.458404647655787	24.297140916793268
100	24.547896391453484	25.77778896345666	25.372884903332448	24.301429741757403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64019.0
1	41103.0
2	19040.0
3	18771.0
4	14162.5
5	7825.5
6	4037.0
7	3024.0
8	2860.0
9	2760.0
10	2800.0
11	2923.0
12	3180.5
13	3591.5
14	4244.0
15	5349.5
16	7327.0
17	10631.5
18	15949.0
19	24728.0
20	37714.5
21	56032.5
22	80763.0
23	113263.0
24	154535.5
25	203862.0
26	262199.5
27	329301.0
28	403332.5
29	484352.0
30	570090.0
31	662595.0
32	760852.5
33	865549.0
34	977466.5
35	1092312.0
36	1217103.0
37	1354890.5
38	1499371.0
39	1639227.0
40	1759160.0
41	1847857.0
42	1895783.5
43	1903050.0
44	1876065.0
45	1820276.0
46	1739931.0
47	1651302.5
48	1565561.0
49	1484297.0
50	1418828.5
51	1368252.0
52	1328717.5
53	1309736.0
54	1311508.5
55	1328244.5
56	1353068.5
57	1366794.0
58	1360154.5
59	1334242.5
60	1292595.0
61	1236682.0
62	1167469.0
63	1085189.0
64	990190.0
65	885460.5
66	777513.5
67	674783.5
68	579246.5
69	490111.5
70	409617.0
71	338410.5
72	276465.0
73	224139.0
74	180721.5
75	146943.0
76	119847.0
77	98157.0
78	80930.5
79	65883.0
80	52997.5
81	42803.0
82	34790.0
83	27895.0
84	21844.5
85	16563.5
86	12397.0
87	9173.5
88	6698.0
89	4723.0
90	3350.5
91	2459.0
92	1719.0
93	1162.0
94	844.5
95	637.0
96	461.5
97	349.0
98	272.5
99	248.0
100	487.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24241851414857113
2	0.1855785660593566
3	0.17987759655504698
4	0.1681957556543884
5	0.1452040724966848
6	0.2105656788345015
7	0.2325045697144058
8	0.2583079125231104
9	0.2678643048629288
10-11	0.26017603192514027
12-13	0.27387768099512744
14-15	0.2736384100229003
16-17	0.2635511069975365
18-19	0.26112137799276974
20-21	0.2648792866207293
22-23	0.2776419099883911
24-25	0.265337964559942
26-27	0.2895278083974396
28-29	0.31508736568727125
30-31	0.315454849784239
32-33	0.2928378739981363
34-35	0.288780199472896
36-37	0.2956937762062661
38-39	0.28149913864255816
40-41	0.28647958650219896
42-43	0.281265285126306
44-45	0.28669718765052626
46-47	0.3028493326398508
48-49	0.30262360530756105
50-51	0.29742104175294715
52-53	0.30520412017029735
54-55	0.3041143086100025
56-57	0.2975709247015876
58-59	0.2944757515212307
60-61	0.3010940769039658
62-63	0.2971917027833408
64-65	0.3041693860790812
66-67	0.29539581612764365
68-69	0.29959524741756194
70-71	0.3033748258694213
72-73	0.29798536008367155
74-75	0.30136856134003015
76-77	0.3061079324087855
78-79	0.29656147207158784
80-81	0.3052212754475514
82-83	0.31087709948540304
84-85	0.31870803209719867
86-87	0.31370952605097474
88-89	0.3237751592524387
90-91	0.33637345312219363
92-93	0.30380009616345516
94-95	0.3038416299925965
96-97	0.30490706300100406
98-99	0.29635199710722293
100	0.33755987598070847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.5376546E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.32564338784572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11730082068968	62.752391332780974
2	14.972381479644085	23.454428248827767
3	3.5858185876290203	8.425846438444177
4	0.8765847200066916	2.7463624871391485
5	0.267086322035258	1.0459854006752467
6	0.08436757414517941	0.3964886715595773
7	0.027119747801545805	0.14869201865505327
8	0.012838494728844426	0.08044666878145644
9	0.007138938151564206	0.0503245731444574
>10	0.0472407102456085	0.7853353660667957
>50	0.002024195776830406	0.10286239244925101
>100	9.545667009967935E-5	0.00889386326752218
>500	2.9524755703501255E-6	0.0019425382085303906
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4085385534879695E-4	5.417455974953729E-6	1.8058186583179095E-6	7.223274633271638E-6	1.8058186583179095E-6
2	1.4446549266543276E-4	9.029093291589548E-6	1.8058186583179095E-6	1.9864005241497006E-5	5.417455974953729E-6
3	1.534945859570223E-4	1.0834911949907457E-5	1.8058186583179095E-6	2.5281461216450733E-5	5.417455974953729E-6
4	1.6252367924861187E-4	1.6252367924861187E-5	3.611637316635819E-6	3.2504735849722375E-5	7.223274633271638E-6
5	1.7516440985683722E-4	2.5281461216450733E-5	5.417455974953729E-6	3.611637316635819E-5	1.0834911949907457E-5
6	1.7697022851515513E-4	3.2504735849722375E-5	7.223274633271638E-6	4.695128511626565E-5	1.2640730608225367E-5
7	1.8058186583179095E-4	3.431055450804028E-5	1.0834911949907457E-5	5.417455974953729E-5	1.2640730608225367E-5
8	1.8238768449010885E-4	4.695128511626565E-5	1.0834911949907457E-5	5.59803784078552E-5	1.2640730608225367E-5
9	1.896109591233805E-4	4.695128511626565E-5	1.0834911949907457E-5	6.862110901608057E-5	1.6252367924861187E-5
10-11	2.0315459906076482E-4	5.688328773701415E-5	1.0834911949907457E-5	8.487347694094175E-5	2.1669823899814914E-5
12-13	2.1940696698562602E-4	6.952401834523952E-5	1.1737821279066411E-5	9.299966090337233E-5	2.7087279874768645E-5
14-15	2.4649424686039464E-4	7.855311163682907E-5	1.9864005241497006E-5	1.1105784748655144E-4	3.1601826520563414E-5
16-17	2.663582521018917E-4	8.487347694094175E-5	2.3475642558132822E-5	1.3453349004468424E-4	3.2504735849722375E-5
18-19	2.9073680398918343E-4	9.299966090337233E-5	2.979600786224551E-5	1.5530040461534024E-4	3.2504735849722375E-5
20-21	3.295619051430185E-4	1.0112584486580293E-4	3.431055450804028E-5	1.7877604717347304E-4	3.2504735849722375E-5
22-23	3.494259103845155E-4	1.1015493815739249E-4	3.431055450804028E-5	1.9864005241497005E-4	3.611637316635819E-5
24-25	4.026975608048938E-4	1.1647530346150516E-4	3.972801048299401E-5	2.112807830231954E-4	3.882510115383506E-5
26-27	4.4874593659200053E-4	1.2098985010729993E-4	4.6951285116265644E-5	2.2663024161889765E-4	4.153382914131192E-5
28-29	4.929884937207893E-4	1.2640730608225366E-4	5.146583176206042E-5	2.5010588417703045E-4	4.153382914131192E-5
30-31	5.607066934077109E-4	1.3092185272804845E-4	5.868910639533206E-5	2.7087279874768645E-4	4.6951285116265644E-5
32-33	6.410656237028579E-4	1.3724221803216113E-4	6.862110901608057E-5	2.8080480136843494E-4	5.236874109121938E-5
34-35	7.015605487565078E-4	1.4446549266543276E-4	6.952401834523952E-5	2.9976589728077297E-4	5.688328773701415E-5
36-37	7.584438364935219E-4	1.4988294864038649E-4	7.042692767439847E-5	3.2053281185142897E-4	5.959201572449101E-5
38-39	8.207445802054899E-4	1.5891204193197605E-4	7.313565566187533E-5	3.4671718239703863E-4	6.139783438280892E-5
40-41	9.227733344004518E-4	1.6432949790692978E-4	7.855311163682907E-5	3.629695503218998E-4	6.500947169944475E-5
42-43	0.0010419573658494337	1.7516440985683722E-4	8.306765828262384E-5	3.8463937422171475E-4	6.681529035776266E-5
44-45	0.0011656559439442106	1.8599932180674467E-4	8.758220492841861E-5	4.108237447673244E-4	6.681529035776266E-5
46-47	0.0013408203538010479	1.959313244274932E-4	9.029093291589547E-5	4.2346447537554977E-4	6.681529035776266E-5
48-49	0.001513276035670408	2.0676623637740064E-4	9.029093291589547E-5	4.433284806170468E-4	7.313565566187533E-5
50-51	0.0016821200802231327	2.2211569497310288E-4	9.390257023253129E-5	4.58677939212749E-4	7.403856499103429E-5
52-53	0.001894303772575487	2.3024187893553347E-4	9.661129822000816E-5	4.7402739780845124E-4	8.035893029514698E-5
54-55	0.002141700928765041	2.4649424686039464E-4	9.751420754916712E-5	4.893768564041535E-4	8.306765828262384E-5
56-57	0.002390903903612912	2.681640707602096E-4	9.932002620748503E-5	5.137554082914452E-4	8.487347694094175E-5
58-59	0.002692475619552003	2.771931640517991E-4	1.0473748218243875E-4	5.300077762163065E-4	8.667929559925966E-5
60-61	0.003100590636331851	2.8441643868507075E-4	1.056403915115977E-4	5.498717814578035E-4	9.390257023253129E-5
62-63	0.0034969678318326316	2.952513506349782E-4	1.1015493815739249E-4	5.634154213951878E-4	9.932002620748503E-5
64-65	0.004010723240124078	3.0428044392656774E-4	1.1015493815739249E-4	5.79667789320049E-4	1.0112584486580293E-4
66-67	0.004596711394748239	3.151153558764752E-4	1.137665754740283E-4	6.067550691948176E-4	1.056403915115977E-4
68-69	0.00534702904727933	3.2324153983890583E-4	1.1737821279066412E-4	6.320365304112684E-4	1.0834911949907458E-4
70-71	0.0062526471044257615	3.331735424596543E-4	1.2008694077814098E-4	6.473859890069706E-4	1.1015493815739249E-4
72-73	0.007468865970802874	3.431055450804028E-4	1.273102154114126E-4	6.609296289443549E-4	1.1286366614486935E-4
74-75	0.009138345320417782	3.59357913005264E-4	1.300189433988895E-4	6.825994528441698E-4	1.1828112211982307E-4
76-77	0.011438958291114798	3.765131902592841E-4	1.318247620572074E-4	7.033663674148258E-4	1.2460148742393575E-4
78-79	0.014583791484575438	3.8825101153835056E-4	1.3543639937384322E-4	7.223274633271638E-4	1.2640730608225366E-4
80-81	0.019218425071148352	4.054062887923707E-4	1.3724221803216113E-4	7.349681939353891E-4	1.273102154114126E-4
82-83	0.026084147610073043	4.1804701940059604E-4	1.417567646779559E-4	7.530263805185683E-4	1.300189433988895E-4
84-85	0.03657775983355842	4.4603720860452367E-4	1.4265967400711485E-4	7.701816577725884E-4	1.3272767138636636E-4
86-87	0.05200306281291	4.613866672002259E-4	1.4446549266543276E-4	7.945602096598802E-4	1.3814512736132008E-4
88	0.06758276328754777	4.6590121384602065E-4	1.4446549266543276E-4	8.072009402681056E-4	1.4085385534879695E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	14735	0.0	10.049585	94
TCCGATC	6415	0.0	10.035543	3
TAGATCG	6720	0.0	9.373963	94
AGATCGG	18755	0.0	8.998396	94
ATCGGAA	15550	0.0	8.676393	94
TTCCGAT	8400	0.0	8.27962	2
CCGATCT	8995	0.0	7.836355	4
CGATCTA	3465	0.0	5.559884	5
CTTCCGA	14560	0.0	5.069084	1
TCGGAAG	20575	0.0	5.0037084	94
>>END_MODULE