##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049540_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55376546
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22124353873569	34.0	31.0	34.0	30.0	34.0
2	32.40252402163183	34.0	31.0	34.0	30.0	34.0
3	32.46239716720505	34.0	31.0	34.0	30.0	34.0
4	35.91902884300512	37.0	35.0	37.0	35.0	37.0
5	35.98954898703867	37.0	35.0	37.0	35.0	37.0
6	36.030694799924866	37.0	35.0	37.0	35.0	37.0
7	36.033677885941096	37.0	35.0	37.0	35.0	37.0
8	36.03518146473058	37.0	35.0	37.0	35.0	37.0
9	37.76066997750275	39.0	38.0	39.0	35.0	39.0
10-11	37.76506673059746	39.0	38.0	39.0	35.0	39.0
12-13	37.74992544677669	39.0	38.0	39.0	35.0	39.0
14-15	39.16928282237032	40.0	39.0	41.0	36.0	41.0
16-17	39.14266850446035	40.0	39.0	41.0	36.0	41.0
18-19	39.15044597581077	40.0	39.0	41.0	36.0	41.0
20-21	39.16238814352921	40.0	39.0	41.0	36.0	41.0
22-23	39.138030214090996	40.0	39.0	41.0	36.0	41.0
24-25	39.11457973561587	40.0	39.0	41.0	36.0	41.0
26-27	39.02703297890771	40.0	39.0	41.0	35.5	41.0
28-29	38.96581919356256	40.0	39.0	41.0	35.0	41.0
30-31	38.90509689788164	40.0	38.5	41.0	35.0	41.0
32-33	38.826998581673905	40.0	38.0	41.0	35.0	41.0
34-35	38.76658606515473	40.0	38.0	41.0	35.0	41.0
36-37	38.693245819267965	40.0	38.0	41.0	35.0	41.0
38-39	38.62270448395247	40.0	38.0	41.0	35.0	41.0
40-41	38.55269554912291	40.0	38.0	41.0	34.0	41.0
42-43	38.46938060203321	40.0	38.0	41.0	34.0	41.0
44-45	38.38040668878121	40.0	38.0	41.0	34.0	41.0
46-47	38.27665328747662	40.0	37.5	41.0	34.0	41.0
48-49	38.15060082476072	40.0	37.0	41.0	33.5	41.0
50-51	38.02923204166616	40.0	37.0	41.0	33.0	41.0
52-53	37.88160933692036	40.0	36.5	41.0	33.0	41.0
54-55	37.70597005996004	40.0	36.0	41.0	33.0	41.0
56-57	37.52299413907108	39.0	35.5	41.0	33.0	41.0
58-59	37.328731318851126	39.0	35.0	41.0	33.0	41.0
60-61	37.087441414276725	39.0	35.0	41.0	32.5	41.0
62-63	36.86756470871261	38.5	35.0	41.0	32.0	41.0
64-65	36.5905687220001	38.0	35.0	40.0	32.0	41.0
66-67	36.3313434084531	37.0	35.0	40.0	32.0	41.0
68-69	36.02846156204831	37.0	35.0	40.0	31.0	41.0
70-71	35.738999413217286	36.0	35.0	39.0	31.0	41.0
72-73	35.42625459160996	36.0	35.0	39.0	31.0	41.0
74-75	35.11928869669842	35.0	34.5	38.5	31.0	40.0
76-77	34.79199224523682	35.0	34.0	37.0	31.0	39.5
78-79	34.49448048818357	35.0	34.0	37.0	31.0	39.0
80-81	34.20922232130549	35.0	34.0	36.5	30.0	39.0
82-83	33.976772296343654	35.0	34.0	36.0	30.0	37.5
84-85	33.751862015734964	35.0	34.0	36.0	30.0	37.0
86-87	33.57135082964546	35.0	34.0	35.0	30.0	37.0
88-89	33.39651901546911	35.0	34.0	35.0	30.0	36.0
90-91	33.23503659834617	35.0	34.0	35.0	30.0	36.0
92-93	33.078173284769335	35.0	34.0	35.0	30.0	36.0
94-95	32.96377785100573	35.0	34.0	35.0	30.0	36.0
96-97	32.83206454407612	35.0	34.0	35.0	29.5	35.0
98-99	32.72237350267386	35.0	34.0	35.0	29.0	35.0
100	32.642979267793265	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26401957099362505
1101	2	0.2295129771795601
1101	3	0.2185172548268639
1101	4	0.18605263108402426
1101	5	0.1944751349527607
1101	6	0.21064184900859573
1101	7	0.2020602740625037
1101	8	0.1773013696805279
1101	9	0.257987749327107
1101	10-11	0.24434179269229617
1101	12-13	0.2718944631921758
1101	14-15	0.3421214507499002
1101	16-17	0.3452774083214507
1101	18-19	0.37335537782947625
1101	20-21	0.3447755684171909
1101	22-23	0.2874655007447231
1101	24-25	0.3123323089513619
1101	26-27	0.34217107674481895
1101	28-29	0.33217495721710577
1101	30-31	0.32853566776560683
1101	32-33	0.3537271506828219
1101	34-35	0.3535563822210719
1101	36-37	0.34008632613389267
1101	38-39	0.37646031509317623
1101	40-41	0.36665638085825947
1101	42-43	0.35120273884962927
1101	44-45	0.3586081469498055
1101	46-47	0.3587698718327488
1101	48-49	0.3823036287568726
1101	50-51	0.3534842970098069
1101	52-53	0.31866047767395855
1101	54-55	0.3162184938478205
1101	56-57	0.36022677535696346
1101	58-59	0.36200212664856934
1101	60-61	0.35103637078103844
1101	62-63	0.34004928156857517
1101	64-65	0.3835698685237787
1101	66-67	0.34753379216135727
1101	68-69	0.3348359201604296
1101	70-71	0.3381777864467992
1101	72-73	0.32196363696710506
1101	74-75	0.32925833255676906
1101	76-77	0.35295534640792425
1101	78-79	0.33860857569999325
1101	80-81	0.35323561647479096
1101	82-83	0.3315313284868182
1101	84-85	0.2805871935469142
1101	86-87	0.2734926772569892
1101	88-89	0.3015402008762038
1101	90-91	0.33431201031991264
1101	92-93	0.37312279410961935
1101	94-95	0.35669455657681937
1101	96-97	0.40752736918988575
1101	98-99	0.42052513577797157
1101	100	0.4163444877166853
1102	1	0.15091876696943274
1102	2	0.13995843055031543
1102	3	0.12229461763029548
1102	4	0.16460406258995164
1102	5	0.1840377027436162
1102	6	0.17567424847062085
1102	7	0.16342383388015946
1102	8	0.1656850420963636
1102	9	0.1941240321110982
1102	10-11	0.18648737781800406
1102	12-13	0.1869899177395027
1102	14-15	0.265184762698766
1102	16-17	0.2834785469994969
1102	18-19	0.31332115742646494
1102	20-21	0.23932184237133924
1102	22-23	0.26924624910193984
1102	24-25	0.26517006695556944
1102	26-27	0.24637928474148652
1102	28-29	0.26042844078267535
1102	30-31	0.2699126304714454
1102	32-33	0.2726843673234285
1102	34-35	0.30727043499339857
1102	36-37	0.27216937473406944
1102	38-39	0.33143844724411053
1102	40-41	0.31734576413862214
1102	42-43	0.3324806882810023
1102	44-45	0.29464430519777807
1102	46-47	0.2990519622195009
1102	48-49	0.2814670239046606
1102	50-51	0.32386327474403487
1102	52-53	0.2816922361890164
1102	54-55	0.2578855015517121
1102	56-57	0.3101890718465796
1102	58-59	0.2823478194609379
1102	60-61	0.2679758495256266
1102	62-63	0.29053930242174886
1102	64-65	0.2988376428300725
1102	66-67	0.27047186646802857
1102	68-69	0.2610546453646805
1102	70-71	0.23899095597231224
1102	72-73	0.2605475680390583
1102	74-75	0.2362883017469528
1102	76-77	0.28232785840774
1102	78-79	0.2420523402634558
1102	80-81	0.276696791655624
1102	82-83	0.24188237259276946
1102	84-85	0.23224832920680427
1102	86-87	0.23237517955514164
1102	88-89	0.2719319813750687
1102	90-91	0.26140086697742504
1102	92-93	0.30963808853562824
1102	94-95	0.29725639946893523
1102	96-97	0.30321988208582695
1102	98-99	0.2887204083040942
1102	100	0.2789305568661504
1103	1	0.16069976349017168
1103	2	0.1616713770013405
1103	3	0.15005627904727703
1103	4	0.14620490248265128
1103	5	0.11638914159036062
1103	6	0.14464717900700919
1103	7	0.1258296658305511
1103	8	0.14365139153602513
1103	9	0.19237561838636452
1103	10-11	0.1824654993411201
1103	12-13	0.1534098361475813
1103	14-15	0.19862568500292355
1103	16-17	0.2056922235250198
1103	18-19	0.20953089236871136
1103	20-21	0.18145482374601585
1103	22-23	0.22109067512280234
1103	24-25	0.23848676819348924
1103	26-27	0.23385603378427788
1103	28-29	0.1982982307996366
1103	30-31	0.25443272876747614
1103	32-33	0.22283801743586906
1103	34-35	0.1874816651939284
1103	36-37	0.20184731837822056
1103	38-39	0.22127501963291962
1103	40-41	0.23126313516716124
1103	42-43	0.25035198887582055
1103	44-45	0.20525788376687615
1103	46-47	0.25466671639388494
1103	48-49	0.2823752617296904
1103	50-51	0.2802871786632224
1103	52-53	0.21943980331999313
1103	54-55	0.2111142598308362
1103	56-57	0.21471301181902902
1103	58-59	0.18336784342372425
1103	60-61	0.21149023184878502
1103	62-63	0.22917159508746465
1103	64-65	0.20189748613655212
1103	66-67	0.21882882650254487
1103	68-69	0.2099750692853064
1103	70-71	0.17082153148685109
1103	72-73	0.20466828665263392
1103	74-75	0.15478266065964874
1103	76-77	0.1474019150460748
1103	78-79	0.146571939855491
1103	80-81	0.13565640888541708
1103	82-83	0.16071416256260562
1103	84-85	0.09133705322618368
1103	86-87	0.1272284410602893
1103	88-89	0.13882053121609772
1103	90-91	0.1194837379732121
1103	92-93	0.19048668133522284
1103	94-95	0.1734981271356304
1103	96-97	0.18563070646391822
1103	98-99	0.2029708887682844
1103	100	0.1856542801428489
1104	1	0.0769343715442119
1104	2	0.07696450902331975
1104	3	0.0851451230454856
1104	4	0.07364254376478385
1104	5	0.07274156804187726
1104	6	0.09783349766505012
1104	7	0.10293086988110645
1104	8	0.11726994611300512
1104	9	0.14729201304314898
1104	10-11	0.1461837124989529
1104	12-13	0.16423058454339667
1104	14-15	0.16575279627880946
1104	16-17	0.14916262925828505
1104	18-19	0.1528498009508752
1104	20-21	0.1113166256349416
1104	22-23	0.1371711859286009
1104	24-25	0.1255156075081274
1104	26-27	0.06190859738167376
1104	28-29	0.11589211071176919
1104	30-31	0.1440281812227937
1104	32-33	0.06048261284045253
1104	34-35	0.09044893986797575
1104	36-37	0.11390675977870757
1104	38-39	0.11248273303886691
1104	40-41	0.10344556883789124
1104	42-43	0.1080451115150396
1104	44-45	0.1280062665531858
1104	46-47	0.1372554740876808
1104	48-49	0.12088164134230084
1104	50-51	0.13838265817165762
1104	52-53	0.1140740811394707
1104	54-55	0.13419966601649236
1104	56-57	0.1822830192653484
1104	58-59	0.13895733938932864
1104	60-61	0.14767227042337794
1104	62-63	0.09214447464105291
1104	64-65	0.09381104742561774
1104	66-67	0.0860615580144426
1104	68-69	0.126973303985622
1104	70-71	0.10078572414673914
1104	72-73	0.08704915745748565
1104	74-75	0.03741459482543519
1104	76-77	0.08246361717874606
1104	78-79	0.0853846503069775
1104	80-81	0.10143749647071587
1104	82-83	0.1024955214196126
1104	84-85	0.037066326456688614
1104	86-87	0.0032915438337468572
1104	88-89	0.05784061414234287
1104	90-91	0.06160654337558924
1104	92-93	0.0853837205561021
1104	94-95	0.09220013630471868
1104	96-97	0.07749189290185399
1104	98-99	0.046319328794837134
1104	100	0.03752580924534499
1105	1	0.03410910559817637
1105	2	0.06118625448195303
1105	3	0.03728707139872256
1105	4	0.05997799101714918
1105	5	0.06281068619814789
1105	6	0.06132281084980207
1105	7	0.09266168684010978
1105	8	0.06977907996861887
1105	9	0.09814063443234744
1105	10-11	0.08695551948541436
1105	12-13	0.08814102557771974
1105	14-15	0.09371027670505327
1105	16-17	0.11196186604571778
1105	18-19	0.0979798721511358
1105	20-21	0.07201717506919181
1105	22-23	0.0884557709522511
1105	24-25	0.0810461259177444
1105	26-27	0.08804688414596029
1105	28-29	0.10358353861325043
1105	30-31	0.09794441017330513
1105	32-33	0.0931408223800716
1105	34-35	0.07659185105273991
1105	36-37	0.09442793311828268
1105	38-39	0.11088546980568026
1105	40-41	0.12558610802411607
1105	42-43	0.07120177396866012
1105	44-45	0.08380142185598771
1105	46-47	0.0787916552860537
1105	48-49	0.06670997388693678
1105	50-51	0.0592781395062687
1105	52-53	0.04120215081778866
1105	54-55	0.06081326942123866
1105	56-57	0.10735439278267478
1105	58-59	0.08740279558919894
1105	60-61	0.10475431840019667
1105	62-63	0.0599809992889746
1105	64-65	0.07240737203232328
1105	66-67	0.04920677010017016
1105	68-69	0.06548520298318294
1105	70-71	0.08277005244660529
1105	72-73	0.061922694726092686
1105	74-75	0.041165576782631774
1105	76-77	0.08461848947560213
1105	78-79	0.06513263031877159
1105	80-81	0.0935011104567991
1105	82-83	0.05802074331268159
1105	84-85	0.03972745506441555
1105	86-87	0.06582015941257424
1105	88-89	0.05320747570733886
1105	90-91	0.05380001911724719
1105	92-93	0.06929772122170164
1105	94-95	0.04522987053606187
1105	96-97	0.061916890564546634
1105	98-99	0.10965198683273769
1105	100	0.09348230293299054
1106	1	0.042731421285765236
1106	2	0.04164109927170756
1106	3	0.01302744930342925
1106	4	0.06954963491165245
1106	5	0.03952248523241053
1106	6	0.038596203559563946
1106	7	0.07971421530814382
1106	8	0.06586538762685024
1106	9	0.1009071888176365
1106	10-11	0.09104352086325207
1106	12-13	0.06258708504061161
1106	14-15	0.11336225900546992
1106	16-17	0.09836079098366213
1106	18-19	0.12113556579057416
1106	20-21	0.06598476240100837
1106	22-23	0.06663571716179462
1106	24-25	0.08650342585928428
1106	26-27	0.0686370222165209
1106	28-29	0.06501196796532582
1106	30-31	0.10226973395266725
1106	32-33	0.11224780224079467
1106	34-35	0.08211070870954273
1106	36-37	0.06788836983521662
1106	38-39	0.07311930879206585
1106	40-41	0.08176546342642155
1106	42-43	0.08188465847033655
1106	44-45	0.10788934445964316
1106	46-47	0.09296621208277145
1106	48-49	0.09608869793967756
1106	50-51	0.08370779840864628
1106	52-53	0.08455766229899098
1106	54-55	0.0721586075127405
1106	56-57	0.08471947229652699
1106	58-59	0.053629497579734675
1106	60-61	0.06095139535897687
1106	62-63	0.08854144780796958
1106	64-65	0.06818239980432139
1106	66-67	0.08314295030237417
1106	68-69	0.07217443822020897
1106	70-71	0.04797948969188326
1106	72-73	0.0381171520928234
1106	74-75	0.036287171665847495
1106	76-77	0.09506945376930531
1106	78-79	0.05737000721953933
1106	80-81	0.06428233692771812
1106	82-83	0.06062117559924474
1106	84-85	0.009828885204200333
1106	86-87	0.07886163251659895
1106	88-89	0.03215685215731412
1106	90-91	0.08434043534936109
1106	92-93	0.09655780549699955
1106	94-95	0.009974885717817017
1106	96-97	0.05175580470210406
1106	98-99	0.054150009825391976
1106	100	0.04062954403745067
1107	1	0.002132014148315875
1107	2	-0.008579582456626156
1107	3	-0.006120141599332385
1107	4	0.05583511212527981
1107	5	0.03714590253644445
1107	6	0.04878720268867198
1107	7	0.04736405513691011
1107	8	0.03967960421699246
1107	9	0.04648871630865159
1107	10-11	0.05089749571583013
1107	12-13	0.04289031195690285
1107	14-15	0.05165257746756424
1107	16-17	0.03284637408515323
1107	18-19	0.02406893338755367
1107	20-21	0.016350203082602377
1107	22-23	0.03723150610846915
1107	24-25	0.006645422207576246
1107	26-27	0.04252045181336683
1107	28-29	0.04572596270941176
1107	30-31	0.05462895552860658
1107	32-33	0.07386321124804596
1107	34-35	0.02014154458760231
1107	36-37	0.04417115752781342
1107	38-39	0.028791065790507275
1107	40-41	0.004857331344688021
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2216	78-79	-0.29848303306480517
2216	80-81	-0.29683932090760123
2216	82-83	-0.34111171265715967
2216	84-85	-0.3706644888374129
2216	86-87	-0.3583712515467923
2216	88-89	-0.3557581492200512
2216	90-91	-0.3399835098022379
2216	92-93	-0.3729356876090719
2216	94-95	-0.40842788897593607
2216	96-97	-0.440574123098024
2216	98-99	-0.4493971362571543
2216	100	-0.4520656087234016
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6592.0
3	1153.0
4	995.0
5	1465.0
6	2571.0
7	5309.0
8	11033.0
9	17649.0
10	23824.0
11	28878.0
12	32140.0
13	35809.0
14	38824.0
15	42006.0
16	45842.0
17	49807.0
18	54383.0
19	59893.0
20	66705.0
21	76426.0
22	89403.0
23	108759.0
24	137904.0
25	180163.0
26	240109.0
27	322805.0
28	431681.0
29	572749.0
30	751273.0
31	979309.0
32	1278780.0
33	1703642.0
34	2411167.0
35	3766299.0
36	6794422.0
37	1.24904E7
38	1.6528803E7
39	5959867.0
40	27707.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.22039217783392	27.065106911439667	25.093442950515087	23.62105796021132
2	23.849808903199442	26.517126388802737	25.5859749602651	24.04708974773272
3	23.87853606832353	25.496514777309898	26.15217672583285	24.472772428533727
4	24.269852131369404	25.364385112587495	26.10345264650413	24.262310109538966
5	24.229009748058562	25.667844765238762	26.06660402114053	24.036541465562138
6	24.10794996134284	25.47878663288245	25.758179284060077	24.655084121714633
7	23.972343815015115	25.752306761783228	26.166973288655456	24.1083761345462
8	23.611719098510825	25.89076155930344	26.250962980710913	24.24655636147482
9	23.556494618262118	25.698100180371526	26.418609332877086	24.32679586848927
10-11	23.534278392877734	25.947871505023084	26.4730396872351	24.044810414864084
12-13	23.440630443076028	25.800072471114394	26.574750617346194	24.18454646846338
14-15	23.483739144103264	25.77402175002718	26.565711861118203	24.176527244751355
16-17	23.550240023998608	25.726600030272746	26.54320747993203	24.17995246579662
18-19	23.572067088297448	25.61007641275398	26.49729677585072	24.32055972309785
20-21	23.617598365615013	25.595383082366286	26.46299955812425	24.32401899389445
22-23	23.717216040776307	25.660742706758207	26.36591132060562	24.25612993185987
24-25	23.76996599544135	25.629224921018363	26.28100567347004	24.319803410070246
26-27	23.852382683313852	25.615315720490617	26.242063318247848	24.29023827794768
28-29	23.88599698438498	25.676535671722444	26.192476096056687	24.244991247835888
30-31	23.934547185234532	25.603926684151133	26.17174545712609	24.28978067348825
32-33	23.982343807706787	25.62026554339079	26.104002318623788	24.293388330278635
34-35	24.009173659394236	25.643242913102153	26.079429578825646	24.26815384867796
36-37	24.03535125853931	25.603067713104604	26.04192541965321	24.319655608702874
38-39	24.04509752974021	25.568239665337554	26.05871040460953	24.327952400312704
40-41	24.08615170121851	25.62596017261206	26.008541176187173	24.279346949982255
42-43	24.12615584384016	25.566523599492168	25.961749355361576	24.3455712013061
44-45	24.131437349529648	25.554478033688742	25.956127793397133	24.357956823384473
46-47	24.180018190220505	25.59604692047191	25.916830390961167	24.307104498346416
48-49	24.19452988203124	25.541875669825586	25.879674169965245	24.38392027817793
50-51	24.187058494783297	25.569857772436695	25.880051580294822	24.363032152485186
52-53	24.208232951768867	25.608912768719193	25.861213462800503	24.321640816711433
54-55	24.237910614326495	25.564100873795358	25.834360228529412	24.363628283348728
56-57	24.25063924744452	25.5639446364245	25.82281296614191	24.362603149989067
58-59	24.264408388824556	25.599564761756245	25.823089909276636	24.31293694014256
60-61	24.283225250125916	25.562037163060992	25.78283223697107	24.371905349842024
62-63	24.278389519978543	25.564244653921637	25.79486710203102	24.3624987240688
64-65	24.310424083763063	25.61662581357985	25.760073686976032	24.31287641568106
66-67	24.31753016137025	25.580922468535526	25.73540286689991	24.366144503194313
68-69	24.310190597657527	25.586320576888248	25.733412847028596	24.370075978425625
70-71	24.355372040742758	25.61565710821056	25.704621562022368	24.32434928902432
72-73	24.36315649455973	25.620218095223795	25.66687759680894	24.34974781340754
74-75	24.387572789396742	25.613204170177244	25.68327770721135	24.315945333214668
76-77	24.39749320507535	25.654121315251093	25.659639059525723	24.28874642014783
78-79	24.405891734337327	25.64109993601021	25.645169444368726	24.30783888528374
80-81	24.397139690489254	25.648769795525677	25.65478331065714	24.299307203327928
82-83	24.41163945443254	25.689734281224297	25.651018883336896	24.24760738100627
84-85	24.417648275938973	25.654281600343698	25.62003293307781	24.308037190639524
86-87	24.410089564639797	25.68612597956034	25.61287664211132	24.290907813688538
88-89	24.438975960177622	25.71620368268171	25.595947371243373	24.2488729858973
90-91	24.45336551549943	25.700925571355025	25.576396017614954	24.269312895530586
92-93	24.448095090526632	25.731367167409257	25.571389770172733	24.24914797189138
94-95	24.46001230108333	25.78233406496539	25.553409927240967	24.20424370671031
96-97	24.472645340505903	25.791004900681603	25.509471695435014	24.226878063377477
98-99	24.471335448456852	25.82555469802641	25.507338989341715	24.195770864175024
100	24.506605278064423	25.868473618264805	25.465097596684767	24.15982350698601
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	33.0
2	29.0
3	32.5
4	38.0
5	56.0
6	87.5
7	105.5
8	169.5
9	254.0
10	316.5
11	439.5
12	650.0
13	963.5
14	1444.5
15	2216.0
16	3647.0
17	6038.0
18	10179.5
19	16720.0
20	26838.0
21	41946.0
22	62470.0
23	90056.0
24	125530.5
25	169259.0
26	221980.0
27	283540.5
28	353797.0
29	432610.5
30	516914.5
31	608926.0
32	709038.0
33	818340.0
34	938421.5
35	1064371.0
36	1198739.0
37	1346575.5
38	1505701.0
39	1660417.5
40	1795136.0
41	1897143.5
42	1953280.5
43	1963567.0
44	1937894.0
45	1879514.5
46	1793431.0
47	1700647.5
48	1608696.5
49	1525050.5
50	1458161.0
51	1405003.5
52	1365951.0
53	1347569.0
54	1351364.0
55	1370233.0
56	1395297.0
57	1409913.0
58	1401313.5
59	1368093.0
60	1320691.0
61	1259495.5
62	1182308.5
63	1093296.0
64	992553.0
65	881561.5
66	770302.5
67	665860.0
68	567186.0
69	476132.5
70	394843.5
71	324112.5
72	262992.5
73	210797.0
74	168174.5
75	135339.0
76	109578.5
77	89053.0
78	72739.0
79	58547.0
80	46754.0
81	37393.0
82	29723.5
83	23385.5
84	17573.0
85	12820.0
86	9278.0
87	6376.0
88	4327.0
89	2821.0
90	1820.0
91	1144.0
92	605.5
93	338.0
94	191.0
95	114.5
96	67.0
97	35.0
98	24.5
99	13.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04537299960889579
2	2.9073680398918343E-4
3	0.0010852970136490635
4	0.08406988763799028
5	0.019972354360996078
6	0.0
7	0.0
8	5.543863281035982E-4
9	5.218815922538759E-4
10-11	0.0
12-13	0.0
14-15	3.160182652056342E-5
16-17	0.0
18-19	8.126183962430594E-4
20-21	2.157953296689902E-4
22-23	1.3363058071552532E-4
24-25	0.0021245456515110207
26-27	0.0032188717584516733
28-29	0.005595329112798042
30-31	0.010947775616052327
32-33	0.004187693468639232
34-35	0.005427387977574477
36-37	0.005036428238048649
38-39	0.00782551515582066
40-41	0.0031367070094982094
42-43	0.005881551370141431
44-45	0.003715471889489099
46-47	0.0021426038380942
48-49	3.431055450804028E-4
50-51	6.500947169944474E-4
52-53	0.0013670047243466575
54-55	8.658900466634377E-4
56-57	0.004422449894220561
58-59	0.001620722245840324
60-61	9.697246195167174E-4
62-63	5.841823359658437E-4
64-65	0.0012306654156436553
66-67	0.0016469066163859335
68-69	0.0013733250896507702
70-71	0.0019114590498295074
72-73	0.0035619773035320767
74-75	7.846282070391317E-4
76-77	0.0011846170398565486
78-79	0.0023593020770923488
80-81	0.002311447882646924
82-83	0.0058309884477085305
84-85	0.0038436850142296703
86-87	0.001116898840169627
88-89	4.379110246420931E-4
90-91	9.652100728709226E-4
92-93	0.005031010782073696
94-95	2.2121278564394393E-4
96-97	0.004724021610159651
98-99	0.0030464160765823135
100	0.0030030764287826838
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.5376546E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.93610319957915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41578712699825	61.89356981205546
2	15.572208299161321	24.272744660975594
3	3.6435873020077856	8.51900987957865
4	0.95810983215628	2.986853870218532
5	0.2529817223369869	0.9858204809831347
6	0.07663281834978192	0.3583477943629924
7	0.025229720643904382	0.13764142782599192
8	0.010644758847869373	0.06636888192817444
9	0.005452740267217354	0.03824687953677173
>10	0.03822233610954919	0.6820848904706034
>50	0.001128580464848611	0.05273402982237364
>100	1.2435227711486357E-5	0.0011048346368652407
>500	0.0	0.0
>1k	2.3274284793851844E-6	0.005472557604817131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4807712998206858E-4	3.611637316635819E-6	1.8058186583179095E-6	1.8058186583179095E-5	3.611637316635819E-6
2	1.4807712998206858E-4	1.2640730608225367E-5	5.417455974953729E-6	2.5281461216450733E-5	3.611637316635819E-6
3	1.5710622327365812E-4	1.4446549266543276E-5	5.417455974953729E-6	3.2504735849722375E-5	3.611637316635819E-6
4	1.6613531656524768E-4	1.8058186583179095E-5	7.223274633271638E-6	3.2504735849722375E-5	5.417455974953729E-6
5	1.697469538818835E-4	2.3475642558132826E-5	7.223274633271638E-6	4.875710377458356E-5	7.223274633271638E-6
6	1.7335859119851931E-4	2.7087279874768645E-5	9.029093291589548E-6	6.139783438280892E-5	7.223274633271638E-6
7	1.7697022851515513E-4	2.8893098533086552E-5	9.029093291589548E-6	7.403856499103429E-5	7.223274633271638E-6
8	1.8419350314842676E-4	3.431055450804028E-5	1.0834911949907457E-5	9.029093291589547E-5	7.223274633271638E-6
9	1.8599932180674467E-4	3.431055450804028E-5	1.2640730608225367E-5	9.209675157421338E-5	7.223274633271638E-6
10-11	2.0044587107328796E-4	4.2436738470470875E-5	1.6252367924861187E-5	1.0654330084075667E-4	7.223274633271638E-6
12-13	2.112807830231954E-4	5.507746907869624E-5	1.715527725402014E-5	1.3092185272804843E-4	9.029093291589548E-6
14-15	2.239215136314208E-4	6.139783438280892E-5	1.9864005241497006E-5	1.4446549266543276E-4	9.932002620748503E-6
16-17	2.356593349104872E-4	6.952401834523952E-5	2.437855188729178E-5	1.5710622327365814E-4	1.0834911949907457E-5
18-19	2.528146121645073E-4	7.223274633271638E-5	2.79901892039276E-5	1.7245568186936036E-4	1.2640730608225367E-5
20-21	2.9254262264750134E-4	8.487347694094175E-5	2.9796007862245506E-5	1.9322259644001633E-4	1.2640730608225367E-5
22-23	3.178240838639521E-4	9.209675157421338E-5	3.611637316635819E-5	2.1308660168151334E-4	1.2640730608225367E-5
24-25	3.7741609958844306E-4	1.0293166352412084E-4	4.063091981215297E-5	2.3475642558132824E-4	1.8058186583179095E-5
26-27	4.2707611269218564E-4	1.1286366614486935E-4	4.5145466457947736E-5	2.4288260954375882E-4	1.8058186583179095E-5
28-29	4.686099418334975E-4	1.1918403144898203E-4	4.695128511626565E-5	2.681640707602096E-4	1.8058186583179095E-5
30-31	5.408426881662138E-4	1.3092185272804843E-4	5.3271650420378335E-5	2.871251666725476E-4	1.8058186583179095E-5
32-33	6.067550691948176E-4	1.4446549266543276E-4	6.500947169944474E-5	3.006688066099319E-4	1.8961095912338053E-5
34-35	6.645412662609907E-4	1.534945859570223E-4	7.132983700355743E-5	3.0969789990152146E-4	2.1669823899814914E-5
36-37	7.232303726563227E-4	1.6252367924861187E-4	7.58443836493522E-5	3.340764517888133E-4	2.2572733228973868E-5
38-39	7.846282070391317E-4	1.6884404455272454E-4	8.306765828262384E-5	3.611637316635819E-4	2.5281461216450737E-5
40-41	8.731133212967093E-4	1.7877604717347304E-4	8.39705676117828E-5	3.972801048299401E-4	2.7087279874768645E-5
42-43	9.76947894149989E-4	1.832905938192678E-4	8.57763862701007E-5	4.1624120074227813E-4	2.7087279874768645E-5
44-45	0.0010861999229782226	1.9864005241497005E-4	9.029093291589547E-5	4.3700811531293413E-4	2.79901892039276E-5
46-47	0.0012541410582017883	2.0405750838992377E-4	9.39025702325313E-5	4.550663018961132E-4	2.9796007862245506E-5
48-49	0.0014284025587294664	2.139895110106723E-4	9.480547956169026E-5	4.78541944454246E-4	3.2504735849722375E-5
50-51	0.0016008582405988268	2.275331509480566E-4	9.751420754916712E-5	5.002117683540609E-4	3.5213463837199237E-5
52-53	0.0018049157489887506	2.4468842820207673E-4	1.0202875419496189E-4	5.146583176206042E-4	3.79221918246761E-5
54-55	0.002055021633165781	2.6003788679777894E-4	1.0473748218243875E-4	5.417455974953729E-4	3.972801048299401E-5
56-57	0.0023024187893553347	2.735815267351633E-4	1.119607568157104E-4	5.715416053576184E-4	4.5145466457947736E-5
58-59	0.002616631235902651	2.8441643868507075E-4	1.1828112211982307E-4	5.98628885232387E-4	4.695128511626565E-5
60-61	0.0030337753459740878	2.9254262264750134E-4	1.2008694077814098E-4	6.18492890473884E-4	4.7854194445424605E-5
62-63	0.0034310554508040284	3.033775345974088E-4	1.2460148742393575E-4	6.365510770570632E-4	5.417455974953729E-5
64-65	0.003943005040437155	3.106008092306804E-4	1.300189433988895E-4	6.564150822985601E-4	5.507746907869624E-5
66-67	0.00453531356036543	3.259502678263827E-4	1.3543639937384322E-4	6.753761782108982E-4	5.59803784078552E-5
68-69	0.005282922484909044	3.4400845440956177E-4	1.3814512736132008E-4	6.934343647940773E-4	5.959201572449101E-5
70-71	0.006195763816688748	3.611637316635819E-4	1.417567646779559E-4	7.142012793647332E-4	6.230074371196787E-5
72-73	0.007434555416294834	3.756102809301252E-4	1.4446549266543276E-4	7.37676921922866E-4	6.681529035776266E-5
74-75	0.00909681149127647	3.9276555818414533E-4	1.4627131132375067E-4	7.647642017976347E-4	7.403856499103429E-5
76-77	0.011407356464594235	4.144353820839602E-4	1.4717422065290962E-4	8.081038495972645E-4	7.674729297851116E-5
78-79	0.014594626396525343	4.361052059837752E-4	1.5078585796954544E-4	8.36996948130351E-4	7.945602096598803E-5
80-81	0.019287949089493592	4.704157604918154E-4	1.516887672987044E-4	8.676958653217556E-4	8.035893029514698E-5
82-83	0.026223195646763522	4.902797657333124E-4	1.516887672987044E-4	8.965889638548421E-4	8.667929559925966E-5
84-85	0.03682515698974797	5.056292243290146E-4	1.516887672987044E-4	9.18258787754657E-4	8.667929559925966E-5
86-87	0.052461740752122746	5.218815922538758E-4	1.516887672987044E-4	9.354140650086771E-4	8.938802358673652E-5
88	0.06831050820684988	5.363281415204192E-4	1.516887672987044E-4	9.462489769585846E-4	9.209675157421338E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	14515	0.0	10.328905	94
AGATCGG	18230	0.0	9.641966	94
ATCGGAA	15605	0.0	8.854504	94
TAGATCG	7190	0.0	8.105382	94
TCGGAAG	20285	0.0	5.2130055	94
>>END_MODULE