FastQCFastQC Report
Wed 25 May 2016
SRR2049539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14337735
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140100.031.7424341
GTATCAA207650.031.5586221
ATCAACG218700.028.4538783
TCAACGC219850.028.3491844
CAACGCA228500.027.337535
TACCTGG119250.027.3115772
AACGCAG242650.025.740216
TATCAAC254800.024.9188442
GTACCTG141400.023.9702761
ACGCAGA287300.021.7259317
GTACATG448350.021.2322031
ACCTGGG155750.020.5806273
CGCAGAG306700.020.1831748
TACATGG458900.019.970482
ACATGGG462700.018.954593
TATAACG13750.018.798882
GCAGAGT368450.016.6229
ATAACGC19500.016.1488823
AGAGTAC311350.015.75986110-11
CATGGGG365200.015.6375624
Produced by FastQC (version 0.11.5)