##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049539_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14337735 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.461417720441897 31.0 31.0 34.0 27.0 34.0 2 30.65466993217548 31.0 31.0 34.0 27.0 34.0 3 30.734363761082207 31.0 31.0 34.0 27.0 34.0 4 34.41327245900416 37.0 35.0 37.0 31.0 37.0 5 34.31497164649786 37.0 35.0 37.0 30.0 37.0 6 34.43085745412368 37.0 35.0 37.0 31.0 37.0 7 34.353442297545605 37.0 35.0 37.0 30.0 37.0 8 34.358562841341396 37.0 35.0 37.0 30.0 37.0 9 35.87333187564145 39.0 37.0 39.0 30.0 39.0 10-11 35.80241021332867 39.0 36.0 39.0 30.0 39.0 12-13 35.73020006298066 39.0 35.0 39.0 30.0 39.0 14-15 36.84074430166271 40.0 37.0 41.0 30.5 41.0 16-17 36.81857957341239 40.0 37.0 41.0 30.0 41.0 18-19 36.805205878055354 40.0 37.0 41.0 30.0 41.0 20-21 36.81034609022973 40.0 37.0 41.0 30.0 41.0 22-23 36.745522776087014 40.0 37.0 41.0 30.0 41.0 24-25 36.726665229898586 40.0 37.0 41.0 30.0 41.0 26-27 36.68813968873047 40.0 37.0 41.0 30.0 41.0 28-29 36.620619539976154 40.0 36.5 41.0 30.0 41.0 30-31 36.55112585774531 40.0 36.0 41.0 30.0 41.0 32-33 36.514253401949475 40.0 36.0 41.0 30.0 41.0 34-35 36.46042697818031 40.0 36.0 41.0 30.0 41.0 36-37 36.36024689394803 40.0 36.0 41.0 30.0 41.0 38-39 36.2720024466905 40.0 36.0 41.0 30.0 41.0 40-41 36.20723283698575 40.0 36.0 41.0 29.0 41.0 42-43 36.01152535599242 39.0 35.0 41.0 29.0 41.0 44-45 35.87081442780188 39.0 35.0 41.0 28.0 41.0 46-47 35.73294892812568 39.0 35.0 41.0 28.0 41.0 48-49 35.59220396387575 39.0 35.0 40.5 28.0 41.0 50-51 35.43261062503945 39.0 35.0 40.0 27.0 41.0 52-53 35.289656176516026 39.0 35.0 40.0 27.0 41.0 54-55 35.09473577939612 38.0 34.0 40.0 27.0 41.0 56-57 34.8895876859211 38.0 34.0 40.0 26.0 41.0 58-59 34.678825630408156 38.0 34.0 40.0 26.0 41.0 60-61 34.441546346058146 37.5 34.0 40.0 26.0 41.0 62-63 34.16775669239249 37.0 33.5 40.0 26.0 41.0 64-65 33.85931215774319 37.0 33.0 40.0 25.0 41.0 66-67 33.59498316854092 36.0 33.0 39.0 25.0 41.0 68-69 33.28295267697443 36.0 33.0 39.0 24.0 41.0 70-71 32.96584314049604 35.0 33.0 39.0 23.5 40.0 72-73 32.61640300926192 35.0 32.0 38.0 23.0 40.0 74-75 32.27190462789276 35.0 32.0 37.0 22.0 39.5 76-77 31.958672726201176 35.0 32.0 37.0 21.5 39.0 78-79 31.625147103081485 35.0 32.0 36.0 20.5 39.0 80-81 31.28178648859112 35.0 31.0 36.0 20.0 38.5 82-83 30.96511987423397 35.0 31.0 35.5 18.5 37.0 84-85 30.65383960576758 34.5 31.0 35.0 17.5 37.0 86-87 30.38562440301763 34.0 31.0 35.0 13.0 36.5 88-89 30.079389701371937 34.0 31.0 35.0 7.0 36.0 90-91 29.812458522911744 34.0 31.0 35.0 2.0 36.0 92-93 29.582478752745814 34.0 30.0 35.0 2.0 35.5 94-95 29.33690795652172 34.0 30.0 35.0 2.0 35.0 96-97 29.075163441087454 34.0 30.0 35.0 2.0 35.0 98-99 28.78959302149189 34.0 30.0 35.0 2.0 35.0 100 28.570063751352638 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3418478891041232 1101 2 0.4366441464874278 1101 3 0.45410964730240266 1101 4 0.42866994470777087 1101 5 0.38465187820391833 1101 6 0.42091349450814164 1101 7 0.42941967973260375 1101 8 0.474698581856444 1101 9 0.5468985765403147 1101 10-11 0.6262882088577157 1101 12-13 0.6025324542489372 1101 14-15 0.7718020364717262 1101 16-17 0.6973615647201967 1101 18-19 0.6882981472136223 1101 20-21 0.7173500649572304 1101 22-23 0.7682942289176253 1101 24-25 0.7498502685370241 1101 26-27 0.7656765549034645 1101 28-29 0.7962060615447726 1101 30-31 0.8188095360538057 1101 32-33 0.8249901081807778 1101 34-35 0.8015391411208483 1101 36-37 0.8014196287195361 1101 38-39 0.7990516789517983 1101 40-41 0.781450350613575 1101 42-43 0.7724748392513874 1101 44-45 0.7131325513416229 1101 46-47 0.6370424088703786 1101 48-49 0.6851506650726265 1101 50-51 0.7238776300668448 1101 52-53 0.7404925975488013 1101 54-55 0.7238383691894015 1101 56-57 0.7441328074964204 1101 58-59 0.7534739390003011 1101 60-61 0.7377584313538463 1101 62-63 0.7096078535010548 1101 64-65 0.5828661018908647 1101 66-67 0.7085996610990151 1101 68-69 0.7464679635745668 1101 70-71 0.7384254358896172 1101 72-73 0.7461324571891126 1101 74-75 0.7856860832783426 1101 76-77 0.6781867256765892 1101 78-79 0.6879694780622572 1101 80-81 0.7203439924623964 1101 82-83 0.7486850484577836 1101 84-85 0.7152543383718317 1101 86-87 0.7095203893412716 1101 88-89 0.7294069888409389 1101 90-91 0.7068499323037578 1101 92-93 0.7479580951596283 1101 94-95 0.7074109692416677 1101 96-97 0.7547573400360825 1101 98-99 0.7312809482077256 1101 100 0.7400449580684203 1102 1 0.30964744219903295 1102 2 0.36872017286302494 1102 3 0.36677349730556585 1102 4 0.3727079961788107 1102 5 0.3350691202508642 1102 6 0.3357701334750729 1102 7 0.30871261157304275 1102 8 0.36105124059511695 1102 9 0.3925269732886747 1102 10-11 0.44205873730740564 1102 12-13 0.46388417207352717 1102 14-15 0.5967597600054333 1102 16-17 0.5759483259878522 1102 18-19 0.525607401941123 1102 20-21 0.5269326508943664 1102 22-23 0.5977638262697695 1102 24-25 0.5250136480831458 1102 26-27 0.6122934287846391 1102 28-29 0.6260113162557062 1102 30-31 0.641382317213484 1102 32-33 0.6252519021670295 1102 34-35 0.6206035672867714 1102 36-37 0.5657172871174083 1102 38-39 0.6145106581369717 1102 40-41 0.5375218590495336 1102 42-43 0.5809159832321953 1102 44-45 0.5770654584117736 1102 46-47 0.5672908114827919 1102 48-49 0.5236181947418075 1102 50-51 0.5413054929968055 1102 52-53 0.5375783505833596 1102 54-55 0.5058693028232071 1102 56-57 0.5663218379772488 1102 58-59 0.5450581253320763 1102 60-61 0.5461447824476267 1102 62-63 0.5270965492751429 1102 64-65 0.45860240434811317 1102 66-67 0.5087111739685852 1102 68-69 0.4988482304785933 1102 70-71 0.5062981216127938 1102 72-73 0.5469933386115642 1102 74-75 0.5223330379565496 1102 76-77 0.5420349763459313 1102 78-79 0.5499532508833056 1102 80-81 0.5692268267361911 1102 82-83 0.5555465561216124 1102 84-85 0.48577277750723624 1102 86-87 0.4461848236811825 1102 88-89 0.47170035925728016 1102 90-91 0.40339646719847977 1102 92-93 0.3669350130820419 1102 94-95 0.385416678876485 1102 96-97 0.4232459267805382 1102 98-99 0.3665077369565388 1102 100 0.400217248167948 1103 1 0.2942097530050418 1103 2 0.3697269956886551 1103 3 0.3546628939800627 1103 4 0.3561763615140521 1103 5 0.31012774209246174 1103 6 0.3736048956671283 1103 7 0.39403954350368764 1103 8 0.4197616674726561 1103 9 0.5000213172935304 1103 10-11 0.5563946259144075 1103 12-13 0.5316045941722081 1103 14-15 0.627486415690143 1103 16-17 0.6647534136812254 1103 18-19 0.6450849904909575 1103 20-21 0.682102333987963 1103 22-23 0.6588933075139067 1103 24-25 0.6368821032794258 1103 26-27 0.6632032991686145 1103 28-29 0.6660722134024226 1103 30-31 0.6614964181026863 1103 32-33 0.6847422480236176 1103 34-35 0.6458660758025019 1103 36-37 0.6087780724610923 1103 38-39 0.6031330337090068 1103 40-41 0.5817988506055514 1103 42-43 0.6390259652746906 1103 44-45 0.6311418899758721 1103 46-47 0.6180073563060802 1103 48-49 0.5224967906252758 1103 50-51 0.5648176262173905 1103 52-53 0.5522036450941528 1103 54-55 0.5704866300908193 1103 56-57 0.5853164508750055 1103 58-59 0.5760540439357698 1103 60-61 0.5552057779919792 1103 62-63 0.5701718700099434 1103 64-65 0.5401916340207222 1103 66-67 0.5376170299085175 1103 68-69 0.5421728056999413 1103 70-71 0.5549307732466318 1103 72-73 0.5045990807476173 1103 74-75 0.528630005602615 1103 76-77 0.5023969124435794 1103 78-79 0.5068753275572675 1103 80-81 0.541011600588206 1103 82-83 0.5679113711909984 1103 84-85 0.5160094558343573 1103 86-87 0.568010384336521 1103 88-89 0.5315336454100823 1103 90-91 0.4931089995960072 1103 92-93 0.5391466878952293 1103 94-95 0.5708754721078755 1103 96-97 0.5735910074557573 1103 98-99 0.5932054593017391 1103 100 0.6757644619762893 1104 1 0.22421827493499435 1104 2 0.2628787397845116 1104 3 0.2944646114344174 1104 4 0.22524857349680616 1104 5 0.2075151908194428 1104 6 0.22869997401952702 1104 7 0.26921608131637953 1104 8 0.3278921522809881 1104 9 0.3891991106485193 1104 10-11 0.4171293127640894 1104 12-13 0.4261110639353376 1104 14-15 0.543854683781106 1104 16-17 0.45867336871437203 1104 18-19 0.5178009858875541 1104 20-21 0.5388927982589422 1104 22-23 0.5181414895959833 1104 24-25 0.545258017264679 1104 26-27 0.5099147243328446 1104 28-29 0.5256443229046468 1104 30-31 0.5980627062550923 1104 32-33 0.5200504528116525 1104 34-35 0.45394499170062375 1104 36-37 0.5575399310228377 1104 38-39 0.5363311039044945 1104 40-41 0.5097874899797858 1104 42-43 0.49254799639896163 1104 44-45 0.47414191304527264 1104 46-47 0.4330606837745492 1104 48-49 0.369914495919609 1104 50-51 0.38696664069230025 1104 52-53 0.3414746439702867 1104 54-55 0.33561677177944915 1104 56-57 0.3807251768037361 1104 58-59 0.4542883616536173 1104 60-61 0.434831777520877 1104 62-63 0.48524496541745066 1104 64-65 0.4003213506224128 1104 66-67 0.40124330678570885 1104 68-69 0.4155768018437058 1104 70-71 0.43150917433633396 1104 72-73 0.43153212334640756 1104 74-75 0.4323442258584791 1104 76-77 0.42072401725438624 1104 78-79 0.416509254023282 1104 80-81 0.42943935928540355 1104 82-83 0.4426907566523184 1104 84-85 0.33413726381930076 1104 86-87 0.38153491314193033 1104 88-89 0.45271310723669345 1104 90-91 0.4774541687331393 1104 92-93 0.5164531776432462 1104 94-95 0.5345448655601466 1104 96-97 0.4724987380710104 1104 98-99 0.4201227829199752 1104 100 0.43454426870113494 1105 1 0.22992885539212082 1105 2 0.27857383404344205 1105 3 0.28219459799051094 1105 4 0.31144677894018713 1105 5 0.21460264898707493 1105 6 0.2526409814403152 1105 7 0.27565993803527533 1105 8 0.28344295003198994 1105 9 0.38549397380461414 1105 10-11 0.4348444995933818 1105 12-13 0.38650644853908744 1105 14-15 0.476317296080353 1105 16-17 0.41922400326963327 1105 18-19 0.40040635579484984 1105 20-21 0.43590436129797183 1105 22-23 0.4248395133149785 1105 24-25 0.4805105310450486 1105 26-27 0.47429777321683986 1105 28-29 0.451955208512949 1105 30-31 0.4340667888545582 1105 32-33 0.507575305644167 1105 34-35 0.5038399127014657 1105 36-37 0.4805899187320719 1105 38-39 0.4988788704120992 1105 40-41 0.4608368744446949 1105 42-43 0.47007749023095613 1105 44-45 0.4256662771742157 1105 46-47 0.37940498474481643 1105 48-49 0.37279291935050907 1105 50-51 0.41207770767704943 1105 52-53 0.3817338164679569 1105 54-55 0.38516339000764077 1105 56-57 0.42650597063748563 1105 58-59 0.42882938538846105 1105 60-61 0.38133676139708683 1105 62-63 0.3722805541268741 1105 64-65 0.31916339432782337 1105 66-67 0.3825510234697447 1105 68-69 0.3803318527792712 1105 70-71 0.40304560169509074 1105 72-73 0.4122012297202531 1105 74-75 0.43779994069418393 1105 76-77 0.3705644899730558 1105 78-79 0.4724386617343299 1105 80-81 0.41841842831421516 1105 82-83 0.4622621844979058 1105 84-85 0.468409114830596 1105 86-87 0.4444833320790167 1105 88-89 0.484184727940427 1105 90-91 0.46325571879990335 1105 92-93 0.5298240374351373 1105 94-95 0.4877786497720855 1105 96-97 0.5268011726807451 1105 98-99 0.41284071867232797 1105 100 0.4670252729662572 1106 1 0.2726963741235373 1106 2 0.3382167009153676 1106 3 0.363457262621651 1106 4 0.31787670280558444 1106 5 0.26489036544268885 1106 6 0.32553936795966365 1106 7 0.27919669906733446 1106 8 0.3268984752781279 1106 9 0.33594615416159 1106 10-11 0.415308158411996 1106 12-13 0.37977468648104207 1106 14-15 0.39107525813140853 1106 16-17 0.40675805434666756 1106 18-19 0.4187240070554097 1106 20-21 0.431796148789914 1106 22-23 0.45813561539317504 1106 24-25 0.47928326438201196 1106 26-27 0.5211192298644107 1106 28-29 0.5005084215768463 1106 30-31 0.4996405569240778 1106 32-33 0.47307496716844355 1106 34-35 0.44351310268915967 1106 36-37 0.4577085339082956 1106 38-39 0.44682910021506217 1106 40-41 0.4626485886653313 1106 42-43 0.4457032351045669 1106 44-45 0.4261110319664354 1106 46-47 0.43652829758476486 1106 48-49 0.42563915793390805 1106 50-51 0.40139743379729254 1106 52-53 0.4197218666817477 1106 54-55 0.392475845199705 1106 56-57 0.3726750051835026 1106 58-59 0.40242457143028787 1106 60-61 0.36499019594376847 1106 62-63 0.39306671537800497 1106 64-65 0.3472385036467003 1106 66-67 0.38151967189713787 1106 68-69 0.34268024996145385 1106 70-71 0.35479764543134706 1106 72-73 0.42922424289945127 1106 74-75 0.462725948326387 1106 76-77 0.4074568059588799 1106 78-79 0.43754015287047565 1106 80-81 0.42369444789342126 1106 82-83 0.4314513899297019 1106 84-85 0.46461668359807007 1106 86-87 0.4307643191031403 1106 88-89 0.46999989778894147 1106 90-91 0.44225122337804734 1106 92-93 0.4610794202307922 1106 94-95 0.44628883727619595 1106 96-97 0.4727461440340228 1106 98-99 0.36730627708151786 1106 100 0.3844892072615096 1107 1 0.15483407906907232 1107 2 0.07434737031073624 1107 3 0.07456208200072112 1107 4 0.05274528383453969 1107 5 0.08880954670296859 1107 6 0.09587648340939836 1107 7 0.006461264121959687 1107 8 0.10160553802799654 1107 9 0.07351485174530836 1107 10-11 0.15187699393297294 1107 12-13 0.19700055986921683 1107 14-15 0.09033733133114197 1107 16-17 0.08362602200180191 1107 18-19 0.14315322308598155 1107 20-21 0.17625774568357144 1107 22-23 0.15079159817278764 1107 24-25 0.1399824997869743 1107 26-27 0.19788650585472567 1107 28-29 0.2197216552631005 1107 30-31 0.25718389487782645 1107 32-33 0.07145943458579751 1107 34-35 0.12281565474710021 1107 36-37 0.13545883314970553 1107 38-39 0.16704145932254022 1107 40-41 0.21997362765016248 1107 42-43 0.14284458741985162 1107 44-45 0.1593047342219478 1107 46-47 0.2170264472994461 1107 48-49 0.20985043057197572 1107 50-51 0.2967766519252635 1107 52-53 0.3069121732096747 1107 54-55 0.2948321363591546 1107 56-57 0.18771630939655637 1107 58-59 0.28456688151513276 1107 60-61 0.25150600321777716 1107 62-63 0.2775057833336021 1107 64-65 0.20206917186073525 1107 66-67 0.3425472094461739 1107 68-69 0.38440766582724706 1107 70-71 0.38450826661167525 1107 72-73 0.3406819004602184 1107 74-75 0.3882321121132932 1107 76-77 0.3124155792965624 1107 78-79 0.41855827704475246 1107 80-81 0.4618618018375855 1107 82-83 0.42169580451259847 1107 84-85 0.44926862423134395 1107 86-87 0.4296482032585871 1107 88-89 0.43677050954513774 1107 90-91 0.4970737076403786 1107 92-93 0.5298278209165233 1107 94-95 0.507975982170354 1107 96-97 0.5144699015482281 1107 98-99 0.4711799304612754 1107 100 0.47809352199594457 1108 1 0.14117701811612804 1108 2 0.1924453371947692 1108 3 0.16764478129117322 1108 4 0.10137537387469564 1108 5 0.11481356182761004 1108 6 0.052345393343841806 1108 7 0.10542752321159554 1108 8 0.09349082371603856 1108 9 0.018473650171635825 1108 10-11 0.13202965761981034 1108 12-13 0.11901670475350556 1108 14-15 0.18922135928777095 1108 16-17 0.14142909120272407 1108 18-19 0.06272089555884719 1108 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2215 52-53 -1.2176277262832258 2215 54-55 -1.2067894917374318 2215 56-57 -1.1214523616489984 2215 58-59 -1.081292695597682 2215 60-61 -1.0400818975455692 2215 62-63 -0.8880548650582938 2215 64-65 -0.9931066596788014 2215 66-67 -0.9854569302727825 2215 68-69 -0.8827209385570924 2215 70-71 -0.8109762805165062 2215 72-73 -0.9165596266625649 2215 74-75 -1.027376108519352 2215 76-77 -1.0455301664308863 2215 78-79 -0.9762130822589405 2215 80-81 -0.8718800603184675 2215 82-83 -0.9645691174348734 2215 84-85 -0.9026169982785532 2215 86-87 -0.8620502862301471 2215 88-89 -1.005440587572 2215 90-91 -1.0435144911266256 2215 92-93 -1.0882265920443217 2215 94-95 -1.0727821326230043 2215 96-97 -1.1160845301901112 2215 98-99 -1.2802740778724413 2215 100 -1.1552709117935542 2216 1 -0.5075774762876435 2216 2 -0.5344312677154477 2216 3 -0.5813678015536397 2216 4 -0.553478102463572 2216 5 -0.4836823550905507 2216 6 -0.5566613140922385 2216 7 -0.5346130113517873 2216 8 -0.6003568365790812 2216 9 -0.7021539442393632 2216 10-11 -0.7506523948975499 2216 12-13 -0.8391209830040793 2216 14-15 -0.946731059782671 2216 16-17 -0.9935788462126851 2216 18-19 -0.9333517005949901 2216 20-21 -0.8887900579960899 2216 22-23 -1.0075640454492287 2216 24-25 -1.0730907422432736 2216 26-27 -1.098205094312533 2216 28-29 -1.069873281856708 2216 30-31 -1.0511561834221368 2216 32-33 -1.0665404670524339 2216 34-35 -1.0644636783340289 2216 36-37 -1.0913051212856217 2216 38-39 -1.0338208576814836 2216 40-41 -1.0157164583398526 2216 42-43 -1.0174164517038875 2216 44-45 -1.0208536730679754 2216 46-47 -0.9911969994511054 2216 48-49 -0.9019430122761634 2216 50-51 -0.9591387038452055 2216 52-53 -1.0351582562522594 2216 54-55 -0.9113180076949803 2216 56-57 -1.0263786677305262 2216 58-59 -1.0275147948303385 2216 60-61 -1.0685830490902717 2216 62-63 -0.8901503305076801 2216 64-65 -1.0202739279513153 2216 66-67 -0.9244885192017804 2216 68-69 -0.8897931739757041 2216 70-71 -1.084644746488543 2216 72-73 -1.0544268567295987 2216 74-75 -0.8609609657456545 2216 76-77 -0.8553248518616243 2216 78-79 -0.8214319752337147 2216 80-81 -0.8714397879029647 2216 82-83 -0.9127449614314394 2216 84-85 -1.0069446092291514 2216 86-87 -1.0077742365932494 2216 88-89 -0.9798786720226857 2216 90-91 -0.9678025681476718 2216 92-93 -0.9385162105275349 2216 94-95 -0.8627166582370833 2216 96-97 -0.8502641810801315 2216 98-99 -0.8397399853212235 2216 100 -0.8847297683949442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 262264.0 3 59091.0 4 34254.0 5 26604.0 6 24556.0 7 24658.0 8 26771.0 9 30322.0 10 33707.0 11 36831.0 12 39385.0 13 41963.0 14 44257.0 15 46552.0 16 47617.0 17 48324.0 18 48173.0 19 49015.0 20 50782.0 21 54276.0 22 62167.0 23 75594.0 24 93398.0 25 115999.0 26 140483.0 27 165289.0 28 202514.0 29 248930.0 30 301497.0 31 365197.0 32 464972.0 33 603138.0 34 824686.0 35 1187072.0 36 1881644.0 37 2938956.0 38 2936309.0 39 699982.0 40 506.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.90841679309408 19.324556041327632 13.856662090489976 23.91036507508832 2 16.10455346098735 20.22274719399301 38.72007729524694 24.952622049772703 3 18.620278134385675 25.073752406449024 29.486020524563138 26.819948934602163 4 13.446975393806776 16.974174583187096 36.895052764744776 32.68379725826136 5 14.282812177020773 36.7748045602765 34.26655618116551 14.67582708153721 6 31.673140007451682 36.18881345848102 17.944224897302053 14.193821636765245 7 27.935615819000777 31.45734526016712 22.28814496515196 18.318893955680146 8 25.807859448110086 34.799446882894436 20.439413831013077 18.953279837982397 9 25.535372790878373 16.919174447186798 20.80608210942678 36.73937065250805 10-11 24.566447968188214 26.40335809712949 28.291172392919982 20.73902154176231 12-13 24.905182036875207 24.05157426966693 28.025285581407168 23.017958112050692 14-15 22.524687848529815 25.931393136325738 25.12923352900801 26.414685486136435 16-17 22.620888002410695 28.861858720237404 25.713252200470798 22.8040010768811 18-19 22.675876059569724 28.295183389021496 27.080360678026878 21.948579873381902 20-21 23.424701096844714 27.59143031977856 26.69503683580694 22.288831747569784 22-23 23.317019771442084 27.33984735293442 26.57452767469108 22.76860520093242 24-25 23.128609836321786 27.431932965947723 26.573699296171505 22.865757901558986 26-27 22.96657247093971 27.450345410327582 26.5952438502561 22.987838268476608 28-29 22.920925417547277 27.437287958545102 26.745575810118105 22.896210813789512 30-31 23.046087615907126 27.573221235854177 26.537514427242865 22.843176720995825 32-33 22.941707193304577 27.542294758871304 26.601502918525277 22.914495129298842 34-35 23.07160890857525 27.28729093907577 26.695074421988522 22.946025730360457 36-37 23.21916433027998 27.30336620875268 26.546994512454425 22.93047494851291 38-39 23.040921898656812 27.44791895906714 26.586132867330818 22.92502627494523 40-41 23.02925863626084 27.240180715075518 26.74912702166779 22.981433626995855 42-43 22.972190643685607 27.349813826107948 26.638980753546328 23.039014776660117 44-45 22.916487060330233 27.447954492022998 26.62424273342006 23.011315714226704 46-47 23.031062190898815 27.260500584389547 26.67796862400939 23.030468600702246 48-49 23.011431557567523 27.240432644611477 26.663802888654526 23.08433290916647 50-51 22.871264853704872 27.28312269440748 26.686345571569092 23.15926688031856 52-53 22.871316523289675 27.298687274355533 26.64994208573601 23.180054116618777 54-55 22.878964981263692 27.403966777628103 26.67903510406798 23.038033137040223 56-57 22.817591248675754 27.240859388262862 26.71726651748462 23.224282845576756 58-59 22.80784297081517 27.019173930925987 26.809350643766162 23.36363245449268 60-61 22.92211331931968 27.18069956597561 26.681975840394166 23.215211274310544 62-63 22.765123614318664 27.245257367722235 26.70577092544535 23.283848092513747 64-65 22.7911916633667 27.081166794294827 26.74717686643828 23.380464675900193 66-67 22.710275423858263 27.099126661750528 26.737936622427107 23.45266129196411 68-69 22.777498267431497 27.102970913376044 26.688285546838834 23.431245272353625 70-71 22.760724404481348 26.902958807180262 26.814297851520024 23.52201893681837 72-73 22.62261109711333 26.99806458241682 26.872671084689543 23.50665323578031 74-75 22.610131070643547 27.010839534192893 26.86091934804319 23.518110047120366 76-77 22.679642536922298 26.976631957058412 26.803011780566322 23.540713725452967 78-79 22.62759046104582 27.021689905460523 26.897174802716716 23.45354483077694 80-81 22.553907523939696 26.897568471135113 26.975263168493836 23.573260836431352 82-83 22.543450288572643 26.813029819743228 27.052591988179962 23.59092790350416 84-85 22.576433404872216 26.79308614601372 26.899110215826212 23.73137023328785 86-87 22.589217028953712 26.86383903560094 26.960907822142673 23.586036113302676 88-89 22.562475060430696 26.723438512543023 27.01280381262946 23.701282614396817 90-91 22.55531722941278 26.69073236710554 26.965965110652597 23.78798529282908 92-93 22.634246847090896 26.73472832572623 26.908931160265176 23.722093666917694 94-95 22.5352830469648 26.465115067105398 27.080184387544946 23.919417498384853 96-97 22.537697547749982 26.462171047640084 27.059777725631427 23.940353678978504 98-99 22.512234358298223 26.545750313570238 27.054432276973923 23.88758305115762 100 22.625502455970807 26.327140600047034 27.008179174280063 24.039177769702096 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8391.0 1 6563.0 2 6075.0 3 7200.0 4 6385.0 5 5507.0 6 5143.0 7 5158.5 8 5388.5 9 5333.5 10 4728.0 11 4083.5 12 3714.0 13 3580.0 14 3624.5 15 3758.5 16 4096.0 17 4689.5 18 5747.0 19 7202.0 20 9274.0 21 12390.0 22 17059.5 23 24078.0 24 33927.5 25 46322.5 26 62717.5 27 83571.0 28 108713.5 29 137850.5 30 166291.5 31 195217.0 32 224804.5 33 250426.5 34 275061.0 35 301891.0 36 331618.5 37 363858.0 38 395308.5 39 421104.0 40 444168.0 41 464372.0 42 477772.0 43 487557.5 44 496714.0 45 504499.5 46 510412.0 47 507011.5 48 499304.5 49 498139.0 50 497289.5 51 493562.5 52 481548.5 53 473052.5 54 465655.0 55 442306.5 56 413007.0 57 377531.0 58 342593.5 59 315228.0 60 285172.0 61 250383.0 62 215031.0 63 180583.0 64 147339.5 65 119155.0 66 94182.0 67 72789.0 68 55908.5 69 42360.0 70 31628.5 71 23819.5 72 18335.0 73 13711.5 74 10586.0 75 7725.0 76 4829.0 77 2970.0 78 1946.5 79 1181.0 80 708.5 81 456.0 82 299.0 83 225.5 84 171.5 85 134.0 86 112.5 87 87.5 88 67.0 89 54.0 90 45.5 91 40.5 92 39.0 93 39.5 94 32.5 95 25.0 96 24.5 97 22.5 98 25.0 99 26.5 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028463352126399326 2 0.05041940027486908 3 0.06312015112568338 4 0.06238084327824443 5 0.05756139306522265 6 0.07510949253839605 7 0.10045519742135002 8 0.08633162769433247 9 0.1023732130632907 10-11 0.10282307491385494 12-13 0.09929043883151698 14-15 0.0926157443975635 16-17 0.10385880336050289 18-19 0.10337755579943415 20-21 0.12061877276989706 22-23 0.11665719864399782 24-25 0.12740157354003265 26-27 0.1322942570775649 28-29 0.11328846571651659 30-31 0.10767739813854837 32-33 0.09210659842715743 34-35 0.10709501884363186 36-37 0.11117864851038187 38-39 0.08911100672456285 40-41 0.09513008853908933 42-43 0.12594736895332492 44-45 0.12710166563965647 46-47 0.12618101813152496 48-49 0.11387433231260027 50-51 0.11374181486824803 52-53 0.10951520585364424 54-55 0.11957258241974761 56-57 0.10437841123441045 58-59 0.08611541502196826 60-61 0.079974277666591 62-63 0.09390604582941448 64-65 0.12652974824824142 66-67 0.11773477470465175 68-69 0.10510028257601357 70-71 0.10205238135591152 72-73 0.11172964209479391 74-75 0.09611002016706265 76-77 0.0891842400490733 78-79 0.08203527265638541 80-81 0.08222707422057947 82-83 0.08527148813951436 84-85 0.07203369290895668 86-87 0.09675168358182097 88-89 0.05647335510106722 90-91 0.02443203197715678 92-93 0.0269882237326886 94-95 0.027225360212055807 96-97 0.02889926477229493 98-99 0.0280692870945097 100 0.027145152285211017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.4337735E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.74325547881 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39640914249208 34.80093411102138 2 15.22249303741878 14.8398773429462 3 5.256225870913428 7.686166814407914 4 2.430174937412395 4.738185513299738 5 1.3861844836421806 3.378357221346656 6 0.8925025348410961 2.610208744274704 7 0.6043668692597702 2.0621166097880286 8 0.4540269769244678 1.7704602344400886 9 0.355653644436158 1.560214480744935 >10 1.8040829437532484 16.119473199552697 >50 0.13304203288393038 4.481691534689102 >100 0.0631860802251699 5.273223382350427 >500 0.0014072980250027243 0.45576681272419584 >1k 2.297893292139155E-4 0.14025556629862723 >5k 0.0 0.0 >10k+ 1.4358443115580862E-5 0.08306843211529667 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.394920466865931E-5 0.0 6.974602334329655E-6 0.0 6 0.0 1.394920466865931E-5 0.0 6.974602334329655E-6 0.0 7 0.0 1.394920466865931E-5 0.0 6.974602334329655E-6 0.0 8 0.0 1.394920466865931E-5 0.0 6.974602334329655E-6 0.0 9 0.0 1.394920466865931E-5 0.0 2.0923807002988966E-5 0.0 10-11 0.0 1.394920466865931E-5 0.0 2.789840933731862E-5 0.0 12-13 0.0 3.138571050448345E-5 0.0 4.184761400597793E-5 0.0 14-15 0.0 6.974602334329655E-5 0.0 6.625872217613172E-5 0.0 16-17 0.0 8.369522801195586E-5 0.0 1.0113173384778E-4 0.0 18-19 0.0 1.0113173384778E-4 0.0 1.1856823968360413E-4 0.0 20-21 0.0 1.1856823968360413E-4 0.0 1.9528886536123035E-4 0.0 22-23 0.0 1.394920466865931E-4 0.0 4.4986185056426276E-4 0.0 24-25 0.0 1.5692855252241722E-4 0.0 0.0010182919408121298 0.0 26-27 0.0 1.778523595254062E-4 0.0 0.0023783393960064127 0.0 28-29 0.0 1.8482696185973587E-4 0.0 0.006092315139036954 0.0 30-31 0.0 1.883142630269007E-4 0.0 0.016090407585298516 0.0 32-33 6.974602334329655E-6 2.0226346769556E-4 0.0 0.040557312574126946 0.0 34-35 1.394920466865931E-5 2.0575076886272483E-4 0.0 0.08263857575830492 0.0 36-37 1.394920466865931E-5 2.301618770328786E-4 0.0 0.1463341315765705 0.0 38-39 1.394920466865931E-5 2.615475875373621E-4 0.0 0.22661877904703914 0.0 40-41 1.394920466865931E-5 2.7549679220602134E-4 0.0 0.30774735339996173 0.0 42-43 1.394920466865931E-5 3.068825027105048E-4 0.0 0.39484618735107047 0.0 44-45 1.394920466865931E-5 3.068825027105048E-4 0.0 0.5074650912434914 0.0 46-47 1.394920466865931E-5 3.1385710504483444E-4 0.0 0.6433338320174002 0.0 48-49 1.394920466865931E-5 3.487301167164828E-4 0.0 0.7810996646262467 0.0 50-51 1.394920466865931E-5 3.6965392371947174E-4 0.0 0.9184749195043709 0.0 52-53 1.394920466865931E-5 3.8360312838813107E-4 0.0 1.0559582807186771 0.0 54-55 1.394920466865931E-5 3.870904295552959E-4 0.0 1.2174412485654114 0.0 56-57 2.789840933731862E-5 3.940650318896255E-4 0.0 1.41110851888391 0.0 58-59 3.487301167164828E-5 3.9755233305679034E-4 0.0 1.6230771457276898 0.0 60-61 3.83603128388131E-5 4.184761400597793E-4 0.0 1.8420482733151367 0.0 62-63 4.184761400597793E-5 4.184761400597793E-4 0.0 2.0663410224836767 0.0 64-65 4.184761400597793E-5 4.289380435612738E-4 0.0 2.3081818711253903 0.0 66-67 4.184761400597793E-5 4.533491517314276E-4 0.0 2.5854746234325017 0.0 68-69 4.184761400597793E-5 4.707856575672517E-4 0.0 2.894184471954601 0.0 70-71 4.184761400597793E-5 4.986840669045703E-4 0.0 3.2228347085505487 0.0 72-73 4.882221634030759E-5 5.091459704060649E-4 0.0 3.558337491939975 0.0 74-75 4.882221634030759E-5 5.126332715732297E-4 0.0 3.9031688059515677 0.0 76-77 4.882221634030759E-5 5.161205727403945E-4 0.0 4.277548022752548 0.0 78-79 4.882221634030759E-5 5.300697774090538E-4 0.0 4.694800120102652 0.0 80-81 4.882221634030759E-5 5.370443797433834E-4 0.0 5.1433019232117205 0.0 82-83 4.882221634030759E-5 5.370443797433834E-4 0.0 5.600835836343746 0.0 84-85 4.882221634030759E-5 5.44018982077713E-4 0.0 6.05700621471941 0.0 86-87 4.882221634030759E-5 5.544808855792076E-4 0.0 6.538191004367147 0.0 88 4.882221634030759E-5 5.579681867463724E-4 0.0 6.92278801358792 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14010 0.0 31.742434 1 GTATCAA 20765 0.0 31.558622 1 ATCAACG 21870 0.0 28.453878 3 TCAACGC 21985 0.0 28.349184 4 CAACGCA 22850 0.0 27.33753 5 TACCTGG 11925 0.0 27.311577 2 AACGCAG 24265 0.0 25.74021 6 TATCAAC 25480 0.0 24.918844 2 GTACCTG 14140 0.0 23.970276 1 ACGCAGA 28730 0.0 21.725931 7 GTACATG 44835 0.0 21.232203 1 ACCTGGG 15575 0.0 20.580627 3 CGCAGAG 30670 0.0 20.183174 8 TACATGG 45890 0.0 19.97048 2 ACATGGG 46270 0.0 18.95459 3 TATAACG 1375 0.0 18.79888 2 GCAGAGT 36845 0.0 16.622 9 ATAACGC 1950 0.0 16.148882 3 AGAGTAC 31135 0.0 15.759861 10-11 CATGGGG 36520 0.0 15.637562 4 >>END_MODULE