FastQCFastQC Report
Wed 25 May 2016
SRR2049539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14337735
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381810.2662972917270406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333430.23255416563355372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191950.13387749180745773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164940.11503909090243333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221800.046.5907861
GTATCAA384900.037.6924931
ATCAACG451400.031.5368523
TCAACGC457650.031.1677824
CAACGCA471700.030.3289875
TATCAAC476000.030.13412
AACGCAG491450.029.2444426
TACCTGG117500.027.8388982
ACGCAGA566250.025.2734367
CGCAGAG586050.024.3554028
GTACCTG145600.023.3994921
GTGGTAT114450.022.6139351
GCAGAGT651200.021.709449
ACCTGGG158900.020.023753
GAGTACT403400.019.75960212-13
GTACATG447000.019.7178481
CAGAGTA626450.019.22483610-11
AGAGTAC598550.019.06485210-11
TATAACG14100.018.6659262
TACATGG458500.018.399492
Produced by FastQC (version 0.11.5)