##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14337735 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6844279797332 31.0 31.0 34.0 30.0 34.0 2 31.79168501858906 33.0 31.0 34.0 30.0 34.0 3 31.876135665779845 33.0 31.0 34.0 30.0 34.0 4 35.53978679338124 37.0 35.0 37.0 33.0 37.0 5 35.62668950151471 37.0 35.0 37.0 33.0 37.0 6 35.71515459031709 37.0 35.0 37.0 33.0 37.0 7 35.67159324677154 37.0 35.0 37.0 33.0 37.0 8 35.68300899688828 37.0 35.0 37.0 33.0 37.0 9 37.32898962074554 39.0 37.0 39.0 34.0 39.0 10-11 37.29351780459048 39.0 37.0 39.0 34.0 39.0 12-13 37.26233167233178 39.0 37.0 39.0 34.0 39.0 14-15 38.52848978586925 40.0 38.0 41.0 34.0 41.0 16-17 38.5513346773392 40.0 38.0 41.0 34.0 41.0 18-19 38.586470596645846 40.0 38.0 41.0 34.0 41.0 20-21 38.611977937937894 40.0 38.0 41.0 34.0 41.0 22-23 38.55728840712986 40.0 38.0 41.0 34.0 41.0 24-25 38.52357185427127 40.0 38.0 41.0 34.0 41.0 26-27 38.42416731094556 40.0 38.0 41.0 34.0 41.0 28-29 38.36007612080988 40.0 38.0 41.0 34.0 41.0 30-31 38.24935382053023 40.0 38.0 41.0 34.0 41.0 32-33 38.117782585603656 40.0 37.5 41.0 33.5 41.0 34-35 38.010879159086144 40.0 37.0 41.0 33.0 41.0 36-37 37.90022667457586 40.0 37.0 41.0 33.0 41.0 38-39 37.80273041732184 40.0 37.0 41.0 33.0 41.0 40-41 37.713502237278064 40.0 37.0 41.0 33.0 41.0 42-43 37.60665132951613 40.0 37.0 41.0 33.0 41.0 44-45 37.45739766427542 40.0 37.0 41.0 32.5 41.0 46-47 37.260569260067925 40.0 36.5 41.0 32.0 41.0 48-49 37.07158058786831 40.0 36.0 41.0 31.0 41.0 50-51 36.915010355540815 39.5 36.0 41.0 31.0 41.0 52-53 36.76611588232032 39.0 35.5 41.0 31.0 41.0 54-55 36.59955760097393 39.0 35.0 41.0 31.0 41.0 56-57 36.39975637714046 39.0 35.0 41.0 30.5 41.0 58-59 36.20053509149109 39.0 35.0 40.5 30.0 41.0 60-61 35.96886994354408 38.0 35.0 40.0 30.0 41.0 62-63 35.7345642111533 38.0 35.0 40.0 30.0 41.0 64-65 35.47718380901865 37.0 34.5 40.0 29.0 41.0 66-67 35.17667410508005 37.0 34.0 40.0 29.0 41.0 68-69 34.87860509348234 36.0 34.0 39.0 29.0 41.0 70-71 34.572786670976974 36.0 34.0 39.0 29.0 41.0 72-73 34.250501386725304 35.0 34.0 39.0 29.0 40.5 74-75 33.93244267661524 35.0 34.0 37.5 28.0 40.0 76-77 33.633309933542506 35.0 33.5 37.0 28.0 39.0 78-79 33.31943002154803 35.0 33.0 37.0 28.0 39.0 80-81 33.01837591502424 35.0 33.0 36.0 27.0 39.0 82-83 32.76756122916207 35.0 33.0 36.0 27.0 37.0 84-85 32.53891873437471 35.0 33.0 35.5 27.0 37.0 86-87 32.31903393388147 35.0 33.0 35.0 26.5 36.5 88-89 32.117111698605115 35.0 33.0 35.0 26.0 36.0 90-91 31.967000784991495 35.0 33.0 35.0 26.0 36.0 92-93 31.802716677355242 35.0 33.0 35.0 26.0 36.0 94-95 31.65692733196701 35.0 33.0 35.0 25.5 35.5 96-97 31.492491666222037 35.0 33.0 35.0 25.0 35.0 98-99 31.312453745309142 35.0 33.0 35.0 25.0 35.0 100 31.19868696136454 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3216863395465879 1101 2 0.315011273470283 1101 3 0.33900885343328113 1101 4 0.2678498831582843 1101 5 0.27494118832571957 1101 6 0.30591512933752796 1101 7 0.26262274658261475 1101 8 0.30650806363667726 1101 9 0.413749236076832 1101 10-11 0.42771315598785264 1101 12-13 0.46632392879222806 1101 14-15 0.5494286113334184 1101 16-17 0.5410613254352867 1101 18-19 0.5705164842710388 1101 20-21 0.5230530813613328 1101 22-23 0.52926032467645 1101 24-25 0.5532340308422476 1101 26-27 0.5725848340698576 1101 28-29 0.5502125610047202 1101 30-31 0.5869102787930345 1101 32-33 0.6367441712493402 1101 34-35 0.6411300707661951 1101 36-37 0.668510583024883 1101 38-39 0.6910006288151962 1101 40-41 0.6793234327931614 1101 42-43 0.6671317626240878 1101 44-45 0.6978527231132219 1101 46-47 0.6904691324617076 1101 48-49 0.7174605517796238 1101 50-51 0.6851742891940376 1101 52-53 0.7083615163955841 1101 54-55 0.6897991492247826 1101 56-57 0.7217883495213755 1101 58-59 0.706358577421625 1101 60-61 0.7258692030654288 1101 62-63 0.7138016822788416 1101 64-65 0.6898448723135076 1101 66-67 0.7092261398247501 1101 68-69 0.6878749736476522 1101 70-71 0.6786230338649588 1101 72-73 0.6396505047267524 1101 74-75 0.6159108398281674 1101 76-77 0.6232991250778639 1101 78-79 0.624342974074608 1101 80-81 0.5972664247512185 1101 82-83 0.5565369735041727 1101 84-85 0.5465506734299055 1101 86-87 0.5700060616464597 1101 88-89 0.5871298501687932 1101 90-91 0.5801221497928424 1101 92-93 0.6202199063082823 1101 94-95 0.6410623104922557 1101 96-97 0.6299428595662917 1101 98-99 0.6655579544368031 1101 100 0.6793444868056717 1102 1 0.30324673853554884 1102 2 0.3245423645697798 1102 3 0.33911314939479453 1102 4 0.3044598220822863 1102 5 0.3058256617947208 1102 6 0.20304430022213893 1102 7 0.23396628007132847 1102 8 0.2234670215461989 1102 9 0.34929178395319127 1102 10-11 0.36926233772887684 1102 12-13 0.37954979227651364 1102 14-15 0.4853829368415745 1102 16-17 0.4360051764679582 1102 18-19 0.39642990491343255 1102 20-21 0.42138477278341924 1102 22-23 0.447406224690198 1102 24-25 0.4522115838008318 1102 26-27 0.4909364935642344 1102 28-29 0.4971674590801243 1102 30-31 0.4241159230359344 1102 32-33 0.475264068769242 1102 34-35 0.5160407097949502 1102 36-37 0.54862022829316 1102 38-39 0.5754675922545758 1102 40-41 0.5969423174497734 1102 42-43 0.5777287121482786 1102 44-45 0.5699378844389216 1102 46-47 0.5291042224396065 1102 48-49 0.6014650122943976 1102 50-51 0.5706274211316611 1102 52-53 0.590856407975771 1102 54-55 0.5763985214572571 1102 56-57 0.5229839635554328 1102 58-59 0.5128919421058242 1102 60-61 0.5384971560644729 1102 62-63 0.542400704530067 1102 64-65 0.577128660926391 1102 66-67 0.5127998220632577 1102 68-69 0.5038452086873519 1102 70-71 0.5068578091960134 1102 72-73 0.48928499032093953 1102 74-75 0.5075103717896994 1102 76-77 0.4875516778004112 1102 78-79 0.44687517629341045 1102 80-81 0.48377349040167417 1102 82-83 0.5375065172468538 1102 84-85 0.4578876729007959 1102 86-87 0.520318794328702 1102 88-89 0.4866626976412505 1102 90-91 0.4775715289382845 1102 92-93 0.4304125925798594 1102 94-95 0.43114794078764973 1102 96-97 0.5377041915022929 1102 98-99 0.5420931155879032 1102 100 0.5632469923273149 1103 1 0.2868445507044086 1103 2 0.24066060957161284 1103 3 0.2708746123017427 1103 4 0.25534967780454565 1103 5 0.23429754654595314 1103 6 0.23029958507083137 1103 7 0.23434415040617296 1103 8 0.2707397339139632 1103 9 0.3775316132471289 1103 10-11 0.3371311810339179 1103 12-13 0.3393374278077985 1103 14-15 0.4412761116463315 1103 16-17 0.3899719565896618 1103 18-19 0.3817815471335564 1103 20-21 0.41026761927520994 1103 22-23 0.4592765112934245 1103 24-25 0.41896953925200364 1103 26-27 0.46421140601239586 1103 28-29 0.46025761969301726 1103 30-31 0.4478358848160937 1103 32-33 0.4893089493028313 1103 34-35 0.4661717874528577 1103 36-37 0.4759545601544133 1103 38-39 0.5208814963310644 1103 40-41 0.47514665731613803 1103 42-43 0.49584252170672727 1103 44-45 0.5013743623129443 1103 46-47 0.523925814622956 1103 48-49 0.5367621221922008 1103 50-51 0.4940711843176331 1103 52-53 0.4873249582706478 1103 54-55 0.5110987384528087 1103 56-57 0.5254148983959936 1103 58-59 0.504361040452352 1103 60-61 0.5453543037555306 1103 62-63 0.45605203065932187 1103 64-65 0.4861660450254277 1103 66-67 0.5389437904839269 1103 68-69 0.4861391550351968 1103 70-71 0.48906395764527844 1103 72-73 0.49246349426776703 1103 74-75 0.4597791897391019 1103 76-77 0.46115930082879686 1103 78-79 0.4807923808517742 1103 80-81 0.4357367088230646 1103 82-83 0.45556773801688166 1103 84-85 0.45700862497128014 1103 86-87 0.4046109377249181 1103 88-89 0.45090971566845894 1103 90-91 0.43277534472326096 1103 92-93 0.45644466577313736 1103 94-95 0.5034861546087264 1103 96-97 0.48926957476131605 1103 98-99 0.5361121902270547 1103 100 0.5389291073227795 1104 1 0.20956580652508094 1104 2 0.20060446716409075 1104 3 0.24478479914195006 1104 4 0.21571127621908914 1104 5 0.20300384437548047 1104 6 0.19789610311258343 1104 7 0.21207786434590048 1104 8 0.20820392425909517 1104 9 0.27006615429993985 1104 10-11 0.2987606404569263 1104 12-13 0.29490812023898627 1104 14-15 0.37260430406968226 1104 16-17 0.36418666377306863 1104 18-19 0.3371863396655641 1104 20-21 0.3345025841390523 1104 22-23 0.3910888066465006 1104 24-25 0.34386533280726894 1104 26-27 0.3570830416554429 1104 28-29 0.3619030691727332 1104 30-31 0.33758287366902096 1104 32-33 0.3219773620264661 1104 34-35 0.38580068075199137 1104 36-37 0.4365327080392234 1104 38-39 0.4428138926000784 1104 40-41 0.4389020777116599 1104 42-43 0.45905844254664174 1104 44-45 0.3989200103999906 1104 46-47 0.41727274644248524 1104 48-49 0.4581623328067792 1104 50-51 0.4273198503844995 1104 52-53 0.4526547898670543 1104 54-55 0.42163771991356924 1104 56-57 0.43012533428259303 1104 58-59 0.4065263461319688 1104 60-61 0.4662009132693754 1104 62-63 0.4533539529243882 1104 64-65 0.45749236268433435 1104 66-67 0.48701126877237755 1104 68-69 0.4520063489385535 1104 70-71 0.4510354090725386 1104 72-73 0.4947445829766508 1104 74-75 0.46173922933254374 1104 76-77 0.4156387935735495 1104 78-79 0.4527033487865566 1104 80-81 0.44124697618707387 1104 82-83 0.404382028980244 1104 84-85 0.3828497912672191 1104 86-87 0.3944783922222541 1104 88-89 0.45013046836575654 1104 90-91 0.4069980639041262 1104 92-93 0.4433075782626652 1104 94-95 0.4851140322529339 1104 96-97 0.4905737475169971 1104 98-99 0.4410795362896245 1104 100 0.4492568770649328 1105 1 0.1689682997251225 1105 2 0.13751059416152245 1105 3 0.21414040960417324 1105 4 0.18223740126038024 1105 5 0.15700658838333936 1105 6 0.15147520093674416 1105 7 0.18460278565987664 1105 8 0.1878952230988702 1105 9 0.2779050722133647 1105 10-11 0.2601479983157944 1105 12-13 0.2647012112440521 1105 14-15 0.3151057735216227 1105 16-17 0.27164877350676164 1105 18-19 0.27859689672302324 1105 20-21 0.31063674754498294 1105 22-23 0.32330126142457516 1105 24-25 0.33635477382239287 1105 26-27 0.3821323924871223 1105 28-29 0.35440899544217785 1105 30-31 0.36295279642470035 1105 32-33 0.40835918338031973 1105 34-35 0.42508245301458913 1105 36-37 0.4248680789720751 1105 38-39 0.4109167618019782 1105 40-41 0.4442609934535682 1105 42-43 0.4037815347319125 1105 44-45 0.3901188934784443 1105 46-47 0.4339314825144527 1105 48-49 0.3919830456424336 1105 50-51 0.43363583592849864 1105 52-53 0.4424176057163791 1105 54-55 0.41290850162002357 1105 56-57 0.40558318115596137 1105 58-59 0.4059607666029308 1105 60-61 0.3857011383469455 1105 62-63 0.37300518928256565 1105 64-65 0.4210236665254641 1105 66-67 0.40462450521107485 1105 68-69 0.42753228377811325 1105 70-71 0.4721637476886045 1105 72-73 0.45630079043150573 1105 74-75 0.44208697679788855 1105 76-77 0.38487775084173137 1105 78-79 0.40550737732985453 1105 80-81 0.40448769635576554 1105 82-83 0.44808659201579104 1105 84-85 0.3977262214458719 1105 86-87 0.4217928156158308 1105 88-89 0.4414506782216634 1105 90-91 0.4164808543755498 1105 92-93 0.4194322081107238 1105 94-95 0.4718836353879716 1105 96-97 0.4504632115396845 1105 98-99 0.4494188037622422 1105 100 0.45011431739038343 1106 1 0.17133562833629057 1106 2 0.1633590817991113 1106 3 0.1763695786517765 1106 4 0.18160827001760538 1106 5 0.15548765203800485 1106 6 0.13810426802521647 1106 7 0.19011273470771783 1106 8 0.16631788592250984 1106 9 0.21129665485751303 1106 10-11 0.18696010476958946 1106 12-13 0.2644375150397025 1106 14-15 0.31777166276067703 1106 16-17 0.29949102796840066 1106 18-19 0.31607231028587535 1106 20-21 0.27618398748889206 1106 22-23 0.2701740858402175 1106 24-25 0.2807148276905167 1106 26-27 0.24726799716208347 1106 28-29 0.2883511350869554 1106 30-31 0.266706019117116 1106 32-33 0.28173006578511917 1106 34-35 0.2728111798864674 1106 36-37 0.26299861191109386 1106 38-39 0.23234736347851737 1106 40-41 0.23088391779464246 1106 42-43 0.27912282459549687 1106 44-45 0.28549602405001906 1106 46-47 0.2800950446019286 1106 48-49 0.28362168375731756 1106 50-51 0.33432239306110745 1106 52-53 0.33163300856213596 1106 54-55 0.31194283701236714 1106 56-57 0.3004383259574297 1106 58-59 0.32484684162187705 1106 60-61 0.35462000513268777 1106 62-63 0.29615780065005026 1106 64-65 0.245235241605414 1106 66-67 0.2458069949664008 1106 68-69 0.28110520414043805 1106 70-71 0.3161331010610766 1106 72-73 0.30836429147568367 1106 74-75 0.3089560371184561 1106 76-77 0.24694405715895584 1106 78-79 0.2805923589424424 1106 80-81 0.2877192733925895 1106 82-83 0.25943017918362443 1106 84-85 0.20960080768690403 1106 86-87 0.2173859981521744 1106 88-89 0.2330233634052803 1106 90-91 0.29456058989649136 1106 92-93 0.3060473804738173 1106 94-95 0.2543369801938553 1106 96-97 0.3063136632982548 1106 98-99 0.2912455230504811 1106 100 0.32053150947485776 1107 1 0.1435339729789682 1107 2 0.12451048810516596 1107 3 0.17304379584882312 1107 4 0.1442911450030948 1107 5 0.12586994937271356 1107 6 0.08036030869129718 1107 7 -0.02658705325973898 1107 8 0.001022086526319299 1107 9 0.027314506203268252 1107 10-11 -4.940432502138492E-4 1107 12-13 0.16320475314043392 1107 14-15 -0.08013307658387703 1107 16-17 0.06891694723442043 1107 18-19 0.0841248766812086 1107 20-21 0.12791318476177338 1107 22-23 0.012020887796026614 1107 24-25 0.16571640957168654 1107 26-27 0.031120053116367785 1107 28-29 0.07478232213522773 1107 30-31 0.0496046530592551 1107 32-33 0.11340064329515798 1107 34-35 0.1382049010849684 1107 36-37 0.05654784393700396 1107 38-39 0.12823992810012186 1107 40-41 0.19511374900855571 1107 42-43 0.06508366829353207 1107 44-45 0.1550719777605849 1107 46-47 0.23342536746665843 1107 48-49 0.15364350895416123 1107 50-51 0.1395053661826111 1107 52-53 0.19672037049529223 1107 54-55 0.23425167381554957 1107 56-57 0.15271411349702646 1107 58-59 0.12674454044505978 1107 60-61 0.1730392942725416 1107 62-63 0.1397411414379235 1107 64-65 0.15713746684217256 1107 66-67 0.08685085224337996 1107 68-69 0.1393994161600176 1107 70-71 0.19273242775626898 1107 72-73 0.128857804958443 1107 74-75 0.11452587583671203 1107 76-77 0.14676195621197508 1107 78-79 0.14352412476047505 1107 80-81 0.1433071842321354 1107 82-83 0.22086391352019774 1107 84-85 0.09558052296564767 1107 86-87 0.11821725607710931 1107 88-89 0.18376210909784163 1107 90-91 0.0613581202511142 1107 92-93 0.1081416202254708 1107 94-95 0.13089192762698332 1107 96-97 0.16890031934354255 1107 98-99 0.294909345223374 1107 100 0.20513483945064337 1108 1 0.09027033946424012 1108 2 0.047959229482838595 1108 3 0.06900697378465637 1108 4 0.060198507637622356 1108 5 0.059182492060159575 1108 6 0.06336361420395775 1108 7 0.06656137189531819 1108 8 0.0601269724223954 1108 9 0.1427509170439194 1108 10-11 0.12113644101295051 1108 12-13 0.09374751924259073 1108 14-15 0.12272625319929631 1108 16-17 0.12003967648004021 1108 18-19 0.1033908583149028 1108 20-21 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-0.720070138604413 2215 24-25 -0.7090641535440056 2215 26-27 -0.6827578218275931 2215 28-29 -0.7045319327318182 2215 30-31 -0.700745861421062 2215 32-33 -0.7056672432497422 2215 34-35 -0.6745811146563554 2215 36-37 -0.7845850316904546 2215 38-39 -0.7603538873669251 2215 40-41 -0.7854464603597435 2215 42-43 -0.7349126785600006 2215 44-45 -0.7386588886075174 2215 46-47 -0.7889546106381218 2215 48-49 -0.7508610572532035 2215 50-51 -0.7985990486902352 2215 52-53 -0.6799004393696464 2215 54-55 -0.6975233077422303 2215 56-57 -0.6433122668204447 2215 58-59 -0.8082549879188292 2215 60-61 -0.6624116640672142 2215 62-63 -0.6477396665059132 2215 64-65 -0.7964167473418939 2215 66-67 -0.7151104277976543 2215 68-69 -0.7532498783468355 2215 70-71 -0.7132948334882698 2215 72-73 -0.6716321389182127 2215 74-75 -0.6681544478042767 2215 76-77 -0.6206743324583499 2215 78-79 -0.6769695532880249 2215 80-81 -0.6878808689678593 2215 82-83 -0.5841558988937123 2215 84-85 -0.6843888425972651 2215 86-87 -0.6415571990415181 2215 88-89 -0.5759980109137253 2215 90-91 -0.6111878563309077 2215 92-93 -0.6584229802972601 2215 94-95 -0.7694282778408841 2215 96-97 -0.8116409218390999 2215 98-99 -0.7724250324524427 2215 100 -0.7599682989496976 2216 1 -0.40795579812665395 2216 2 -0.4163830018769019 2216 3 -0.40483863596016434 2216 4 -0.31415943429697535 2216 5 -0.3831610983995688 2216 6 -0.368231685858575 2216 7 -0.40291316209657424 2216 8 -0.38470575467825796 2216 9 -0.4498701048944156 2216 10-11 -0.47006915757936696 2216 12-13 -0.5224581024415045 2216 14-15 -0.6768921332541993 2216 16-17 -0.7186846286221638 2216 18-19 -0.6957658446413717 2216 20-21 -0.762169497841974 2216 22-23 -0.7718660652569937 2216 24-25 -0.7890269919266046 2216 26-27 -0.9357844044576211 2216 28-29 -0.7490118044006735 2216 30-31 -0.6713411927776676 2216 32-33 -0.7800884969707695 2216 34-35 -0.8728146727302786 2216 36-37 -0.8463552048649134 2216 38-39 -0.8411828392942979 2216 40-41 -0.877009882049947 2216 42-43 -0.8351589257117311 2216 44-45 -0.7760011504591944 2216 46-47 -0.9332275206658878 2216 48-49 -0.9204412510656823 2216 50-51 -0.8755053604132286 2216 52-53 -0.9193175079939451 2216 54-55 -0.9375257687844467 2216 56-57 -0.9569063909325664 2216 58-59 -0.904732512775162 2216 60-61 -1.0058657644961215 2216 62-63 -0.9927220674199475 2216 64-65 -0.8457567030779813 2216 66-67 -0.9420302642169602 2216 68-69 -0.8820455702694687 2216 70-71 -0.9032885903818269 2216 72-73 -0.983032962867739 2216 74-75 -0.9664947395288479 2216 76-77 -0.8646132293979605 2216 78-79 -0.8216173344544018 2216 80-81 -0.8039975608842838 2216 82-83 -0.8067913508671936 2216 84-85 -0.8833686566722108 2216 86-87 -0.8985958199588104 2216 88-89 -1.0269619011461693 2216 90-91 -0.8719029357574648 2216 92-93 -1.029857671151344 2216 94-95 -1.0292312280388103 2216 96-97 -1.106117585501412 2216 98-99 -1.0647530685105515 2216 100 -1.0508492536908989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1380.0 3 126.0 4 213.0 5 547.0 6 1515.0 7 3037.0 8 4954.0 9 7365.0 10 10680.0 11 15391.0 12 20679.0 13 26533.0 14 30221.0 15 38079.0 16 48159.0 17 64155.0 18 62909.0 19 41880.0 20 35480.0 21 36415.0 22 39413.0 23 44851.0 24 54775.0 25 69214.0 26 90773.0 27 120386.0 28 160011.0 29 209923.0 30 272477.0 31 349139.0 32 446689.0 33 573017.0 34 775592.0 35 1117833.0 36 1801757.0 37 3123890.0 38 3590975.0 39 1045578.0 40 1724.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.534319866456386 19.53032050034296 13.978553876761863 23.956805756438794 2 16.044772762224994 20.47891804388908 38.51891529589576 24.957393897990162 3 18.55120073010137 25.300551307441516 29.692123616456854 26.45612434600026 4 13.39591939789044 17.125290931042105 36.78207425376232 32.696715417305136 5 14.153162964722114 36.54483780039176 34.281411952445765 15.020587282440356 6 31.385333875957393 36.51396821046002 17.655054999970357 14.445642913612227 7 27.575588663534788 31.79447070150286 22.276228904264634 18.353711730697714 8 25.90473320925516 34.57016746368935 20.306896451915176 19.21820287514032 9 25.582827413116505 16.388041765313698 21.007641723047605 37.02148909852219 10-11 24.622379126063706 26.183452928068725 28.21572415209189 20.978443793775682 12-13 25.25496993200639 23.707525441196132 28.3669432783051 22.670561348492374 14-15 22.64991200647384 25.344569675650614 25.954207098382742 26.051311219492803 16-17 22.23768259072999 28.365526354058012 26.557664094084593 22.839126961127405 18-19 22.251418860730116 27.216152440628772 28.77709191832203 21.755336780319087 20-21 22.978580647501158 26.443151585658402 28.65457479859964 21.9236929682408 22-23 22.83474089916285 26.207636451048838 28.600271101046253 22.35735154874206 24-25 22.68904022965197 26.310954225523897 28.549413676695814 22.45059186812832 26-27 22.57256014152687 26.355111140498227 28.563221733893883 22.50910698408102 28-29 22.51821085816315 26.32840573038723 28.696849542461777 22.456533868987837 30-31 22.669626430278946 26.46103367909825 28.49513882401009 22.374201066612713 32-33 22.581195704900388 26.414001235202072 28.54059933455319 22.464203725344344 34-35 22.68532987633783 26.237638264069158 28.55019682039458 22.52683503919843 36-37 22.859044532420533 26.25093337622249 28.377486288977043 22.51253580237993 38-39 22.69908043355523 26.393956228093213 28.378750200083903 22.528213138267656 40-41 22.70829761742519 26.224919062928027 28.45920979416338 22.607573525483406 42-43 22.707795823717678 26.352288870314737 28.285252028773318 22.654663277194267 44-45 22.70906511814827 26.48851674701217 28.133704783945944 22.66871335089361 46-47 22.87926579756147 26.381708826394128 27.981407105097144 22.75761827094726 48-49 22.921821333704383 26.466289828902546 27.796367417866215 22.815521419526863 50-51 22.786235064325012 26.59730424645176 27.70613350016582 22.910327189057405 52-53 22.77436080385082 26.63485201811862 27.621562959560908 22.969224218469652 54-55 22.750033390908676 26.759498623736594 27.63124022029979 22.85922776505494 56-57 22.690800544610852 26.557608025400665 27.671485216783843 23.080106213204644 58-59 22.638715250351606 26.360223563903222 27.80474042796857 23.196320757776594 60-61 22.773181785432886 26.4804275543421 27.712142498399157 23.03424816182586 62-63 22.58642677095932 26.536560301506572 27.775727825264955 23.10128510226915 64-65 22.60967191571211 26.38435558573547 27.793809307729443 23.212163190822974 66-67 22.560643182601652 26.408865173223766 27.765207940531972 23.265283703642613 68-69 22.627159602448703 26.48594468754807 27.631964618399223 23.254931091604 70-71 22.63490473484962 26.317113746395655 27.656755730158306 23.391225788596415 72-73 22.520176816605726 26.469937030609657 27.655222893270636 23.354663259513977 74-75 22.55467671011854 26.48047668500247 27.56375975074202 23.40108685413698 76-77 22.612807804673366 26.469432070294737 27.45190030508191 23.465859819949987 78-79 22.573184054820686 26.55158691506802 27.483731926547293 23.391497103564 80-81 22.479946768832566 26.489435196528817 27.52008461746899 23.510533417169633 82-83 22.463610555340836 26.434541475020225 27.531711221496796 23.570136748142147 84-85 22.50614276490785 26.434861931903665 27.351835547918448 23.70715975527004 86-87 22.474933043187146 26.522252678272512 27.38077851244281 23.622035766097536 88-89 22.360428127961303 26.39749035748149 27.43138358858625 23.81069792597096 90-91 22.298373519742892 26.35423221925966 27.408203701582188 23.93919055941526 92-93 22.343488958998236 26.448307026634172 27.325599217834956 23.88260479653264 94-95 22.268859589715163 26.222511332904713 27.465591171207944 24.043037906172184 96-97 22.24758914814487 26.25857650743672 27.402438642483762 24.091395701934644 98-99 22.163031764698253 26.384361685677955 27.34325616456576 24.109350385058033 100 22.24719601101962 26.183824894078196 27.28269020014997 24.28628889475222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4162.0 1 2899.5 2 7223.0 3 10961.5 4 5902.5 5 2684.5 6 4532.0 7 10043.0 8 16680.0 9 19053.5 10 14106.5 11 8328.5 12 6961.0 13 7277.0 14 8024.5 15 9069.5 16 10090.5 17 11065.0 18 12334.0 19 13861.0 20 15865.5 21 18744.5 22 22862.0 23 29372.5 24 38441.0 25 50035.5 26 65089.5 27 84687.5 28 109187.0 29 137990.5 30 166405.0 31 194066.5 32 223034.0 33 248035.0 34 271719.0 35 298275.0 36 327056.5 37 359887.0 38 391742.5 39 415531.5 40 438373.0 41 460878.0 42 475145.5 43 484856.5 44 494499.0 45 502879.0 46 508458.5 47 505561.5 48 498955.0 49 496937.5 50 495863.0 51 492507.0 52 480134.5 53 471725.0 54 465172.0 55 441015.5 56 411577.5 57 376205.5 58 340303.5 59 314731.0 60 285109.0 61 247497.0 62 211370.0 63 176858.0 64 142660.5 65 115045.0 66 89992.5 67 68273.5 68 52397.0 69 39388.5 70 29103.0 71 21376.5 72 16000.0 73 11875.0 74 8963.5 75 6322.0 76 3699.5 77 2180.0 78 1345.0 79 680.0 80 287.5 81 125.5 82 68.0 83 45.5 84 27.0 85 16.5 86 12.5 87 10.0 88 5.5 89 1.5 90 3.5 91 3.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13751125962364347 2 0.0 3 0.0 4 0.0010113173384778001 5 0.0 6 0.0 7 2.0923807002988966E-5 8 0.0 9 0.0 10-11 5.579681867463724E-5 12-13 4.4637454939709794E-4 14-15 2.266745758657138E-4 16-17 0.0 18-19 6.102777042538448E-4 20-21 0.0 22-23 0.0019284775454421498 24-25 2.7549679220602134E-4 26-27 0.002699171103385577 28-29 9.415713151345034E-4 30-31 7.218713416031193E-4 32-33 0.0 34-35 3.068825027105048E-4 36-37 6.974602334329655E-6 38-39 0.0 40-41 3.6965392371947174E-4 42-43 4.882221634030759E-5 44-45 5.091459704060649E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011019871688240854 58-59 0.0 60-61 1.394920466865931E-5 62-63 3.1385710504483446E-5 64-65 1.1856823968360415E-4 66-67 0.0019703251594481276 68-69 7.916173649464159E-4 70-71 0.0012066062038390303 72-73 3.801158272209662E-4 74-75 0.0020958680014660615 76-77 0.0019110410396063254 78-79 0.0026050139718721264 80-81 0.0018831426302690069 82-83 1.8482696185973587E-4 84-85 9.485459174688332E-4 86-87 9.415713151345034E-4 88-89 0.004972891464377044 90-91 0.0034001186379857074 92-93 0.004237070918105266 94-95 0.0019598632559466333 96-97 0.0011717331921673822 98-99 1.8133966069257103E-4 100 2.5108568403586757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.4337735E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.798618108704645 #Duplication Level Percentage of deduplicated Percentage of total 1 69.01767710220906 31.609142363539622 2 16.336727321594445 14.963990714954903 3 5.68700292946009 7.813706260482818 4 2.696080992986357 4.939071351516774 5 1.5280857028422346 3.499210677092151 6 0.9770608743498199 2.6848822711982674 7 0.6597117486904903 2.1149720508071046 8 0.4925548268619781 1.8046664330440696 9 0.3487438124927907 1.4374786217517916 >10 2.0345529301847143 16.840402359995064 >50 0.14723679359892852 4.648929187906415 >100 0.07194796585800402 5.735436395217014 >500 0.001975364853242723 0.595601644638695 >1k 5.194317679764612E-4 0.346198444643075 >5k 6.110112486519068E-5 0.21626325849706118 >10k+ 6.110112486519068E-5 0.7500479647153437 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38181 0.2662972917270406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33343 0.23255416563355372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19195 0.13387749180745773 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16494 0.11503909090243333 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 6.974602334329655E-6 0.0 1.394920466865931E-5 0.0 3 0.0 6.974602334329655E-6 0.0 1.394920466865931E-5 0.0 4 0.0 1.394920466865931E-5 0.0 1.394920466865931E-5 0.0 5 0.0 1.394920466865931E-5 0.0 1.394920466865931E-5 0.0 6 0.0 1.394920466865931E-5 0.0 1.394920466865931E-5 0.0 7 0.0 1.394920466865931E-5 0.0 1.394920466865931E-5 0.0 8 0.0 1.394920466865931E-5 0.0 1.394920466865931E-5 0.0 9 0.0 1.394920466865931E-5 0.0 3.487301167164828E-5 0.0 10-11 0.0 1.394920466865931E-5 0.0 4.533491517314276E-5 0.0 12-13 0.0 2.0923807002988966E-5 0.0 6.27714210089669E-5 0.0 14-15 0.0 2.789840933731862E-5 0.0 8.718252917912068E-5 0.0 16-17 0.0 3.487301167164828E-5 0.0 1.1856823968360413E-4 0.0 18-19 0.0 4.882221634030758E-5 0.0 1.394920466865931E-4 0.0 20-21 0.0 7.323332451046138E-5 0.0 2.3016187703287863E-4 2.0923807002988966E-5 22-23 0.0 9.415713151345034E-5 0.0 4.917094645702407E-4 2.0923807002988966E-5 24-25 0.0 1.0113173384778E-4 0.0 0.001095012566489756 2.0923807002988966E-5 26-27 0.0 1.0461903501494482E-4 0.0 0.002448085419349709 2.0923807002988966E-5 28-29 3.4873011671648277E-6 1.0461903501494482E-4 0.0 0.006217857981054888 2.0923807002988966E-5 30-31 6.974602334329655E-6 1.1159363734927449E-4 0.0 0.016317082161164228 2.0923807002988966E-5 32-33 6.974602334329655E-6 1.2205554085076897E-4 0.0 0.04088511888384044 2.0923807002988966E-5 34-35 6.974602334329655E-6 1.2903014318509863E-4 0.0 0.08331859948590206 2.0923807002988966E-5 36-37 6.974602334329655E-6 1.534412513552524E-4 0.0 0.14763489491192297 2.0923807002988966E-5 38-39 6.974602334329655E-6 1.8482696185973587E-4 0.0 0.2283205820166156 2.0923807002988966E-5 40-41 6.974602334329655E-6 1.987761665283952E-4 0.0 0.30992342932827255 2.0923807002988966E-5 42-43 6.974602334329655E-6 2.3016187703287863E-4 0.0 0.3971966283377395 2.0923807002988966E-5 44-45 6.974602334329655E-6 2.3364917820004345E-4 0.0 0.5102898051888949 2.0923807002988966E-5 46-47 6.974602334329655E-6 2.615475875373621E-4 6.974602334329655E-6 0.6468908792079083 2.0923807002988966E-5 48-49 6.974602334329655E-6 2.789840933731862E-4 6.974602334329655E-6 0.7853716085560236 2.0923807002988966E-5 50-51 6.974602334329655E-6 3.208317073791642E-4 6.974602334329655E-6 0.9234059633547418 2.0923807002988966E-5 52-53 6.974602334329655E-6 3.4873011671648273E-4 6.974602334329655E-6 1.0613670848289498 2.0923807002988966E-5 54-55 6.974602334329655E-6 3.6267932138514205E-4 6.974602334329655E-6 1.223798598593153 2.0923807002988966E-5 56-57 6.974602334329655E-6 3.6267932138514205E-4 6.974602334329655E-6 1.4182888719871025 2.0923807002988966E-5 58-59 6.974602334329655E-6 3.6267932138514205E-4 6.974602334329655E-6 1.6311223495203393 2.0923807002988966E-5 60-61 6.974602334329655E-6 3.661666225523069E-4 6.974602334329655E-6 1.851111769048598 2.0923807002988966E-5 62-63 6.974602334329655E-6 3.6965392371947174E-4 6.974602334329655E-6 2.0768168751898397 2.0923807002988966E-5 64-65 6.974602334329655E-6 3.766285260538014E-4 6.974602334329655E-6 2.319972436371575 2.0923807002988966E-5 66-67 6.974602334329655E-6 4.1150153772544966E-4 6.974602334329655E-6 2.5989669916482625 2.0923807002988966E-5 68-69 6.974602334329655E-6 4.1150153772544966E-4 6.974602334329655E-6 2.9095355716924605 2.0923807002988966E-5 70-71 6.974602334329655E-6 4.2545074239410893E-4 6.974602334329655E-6 3.2402816762898743 2.0923807002988966E-5 72-73 6.974602334329655E-6 4.393999470627683E-4 6.974602334329655E-6 3.578201159388146 2.4411108170153794E-5 74-75 6.974602334329655E-6 4.603237540657572E-4 6.974602334329655E-6 3.9252085493280493 2.789840933731862E-5 76-77 6.974602334329655E-6 4.7427295873441654E-4 6.974602334329655E-6 4.301732456346835 2.789840933731862E-5 78-79 6.974602334329655E-6 4.8473486223591104E-4 6.974602334329655E-6 4.721261761359099 2.789840933731862E-5 80-81 6.974602334329655E-6 5.021713680717351E-4 6.974602334329655E-6 5.1726789482439175 2.789840933731862E-5 82-83 6.974602334329655E-6 5.021713680717351E-4 6.974602334329655E-6 5.63338979273923 2.789840933731862E-5 84-85 6.974602334329655E-6 5.370443797433834E-4 6.974602334329655E-6 6.093814678538835 2.789840933731862E-5 86-87 6.974602334329655E-6 5.44018982077713E-4 6.974602334329655E-6 6.578092704321847 3.138571050448345E-5 88 6.974602334329655E-6 5.509935844120428E-4 6.974602334329655E-6 6.9660444972654325 3.487301167164828E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22180 0.0 46.590786 1 GTATCAA 38490 0.0 37.692493 1 ATCAACG 45140 0.0 31.536852 3 TCAACGC 45765 0.0 31.167782 4 CAACGCA 47170 0.0 30.328987 5 TATCAAC 47600 0.0 30.1341 2 AACGCAG 49145 0.0 29.244442 6 TACCTGG 11750 0.0 27.838898 2 ACGCAGA 56625 0.0 25.273436 7 CGCAGAG 58605 0.0 24.355402 8 GTACCTG 14560 0.0 23.399492 1 GTGGTAT 11445 0.0 22.613935 1 GCAGAGT 65120 0.0 21.70944 9 ACCTGGG 15890 0.0 20.02375 3 GAGTACT 40340 0.0 19.759602 12-13 GTACATG 44700 0.0 19.717848 1 CAGAGTA 62645 0.0 19.224836 10-11 AGAGTAC 59855 0.0 19.064852 10-11 TATAACG 1410 0.0 18.665926 2 TACATGG 45850 0.0 18.39949 2 >>END_MODULE