FastQCFastQC Report
Wed 25 May 2016
SRR2049538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9902914
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139140.14050409808668438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG88500.036.537082
TATAACG12000.030.9410112
GTACCTG108500.030.6313511
GGTATCA97200.029.7913991
GTATCAA142850.027.8398321
ACCTGGG122350.026.3918573
TCAACGC147000.025.5483024
ATCAACG146850.025.5414873
CAACGCA152600.024.6098795
ATAACGC16250.024.5857093
TAACGCA15300.024.5771274
AACGCAG164400.023.1829226
GTACATG299450.022.7154181
TATCAAC170400.022.589312
TACATGG309250.021.018472
ACATGGG306400.020.3103053
GTATAAC28850.019.8788171
ACGCAGA190750.019.6132857
CGCAGAG201000.018.5667138
CATGGGG230050.017.285454
Produced by FastQC (version 0.11.5)