FastQCFastQC Report
Wed 25 May 2016
SRR2049538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9902914
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306410.3094139765325641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263140.2657197669292089No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229590.23184085007705812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155460.15698409579241018No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113590.1147036114824384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162950.048.4307331
GTATCAA276750.036.7799531
TACCTGG92100.035.2615362
TATAACG12850.035.111622
GTACCTG110400.030.6053731
ATCAACG327350.030.50913
TCAACGC331350.030.22594
CAACGCA337700.029.7410495
TATCAAC346850.028.9971222
ATAACGC16650.028.7917963
AACGCAG357500.028.3176236
TAACGCA16450.027.1419164
ACCTGGG121700.026.2990063
ACGCAGA408950.024.6285677
CGCAGAG422000.023.8001258
GTATAAC28500.022.4562051
GCAGAGT468900.021.2993269
GTACATG299000.021.2001381
GTGGTAT81850.021.1578451
TATCACG18100.020.772762
Produced by FastQC (version 0.11.5)