##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9902914 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.686025244690605 31.0 31.0 34.0 30.0 34.0 2 31.774284013776146 33.0 31.0 34.0 30.0 34.0 3 31.864350230649283 33.0 31.0 34.0 30.0 34.0 4 35.52577382778443 37.0 35.0 37.0 33.0 37.0 5 35.61027632876545 37.0 35.0 37.0 33.0 37.0 6 35.69356544952324 37.0 35.0 37.0 33.0 37.0 7 35.644920878844346 37.0 35.0 37.0 33.0 37.0 8 35.64576356009958 37.0 35.0 37.0 33.0 37.0 9 37.28512920540358 39.0 37.0 39.0 34.0 39.0 10-11 37.254955864506144 39.0 37.0 39.0 34.0 39.0 12-13 37.22181269068882 39.0 37.0 39.0 33.5 39.0 14-15 38.46766068048254 40.0 38.0 41.0 34.0 41.0 16-17 38.48003542189703 40.0 38.0 41.0 34.0 41.0 18-19 38.51964926687236 40.0 38.0 41.0 34.0 41.0 20-21 38.54072922374162 40.0 38.0 41.0 34.0 41.0 22-23 38.474199563885946 40.0 38.0 41.0 34.0 41.0 24-25 38.431091343416696 40.0 38.0 41.0 34.0 41.0 26-27 38.32262877371247 40.0 38.0 41.0 34.0 41.0 28-29 38.25365473233434 40.0 38.0 41.0 34.0 41.0 30-31 38.130031675525004 40.0 37.5 41.0 33.5 41.0 32-33 37.97949234942361 40.0 37.0 41.0 33.0 41.0 34-35 37.85653364252179 40.0 37.0 41.0 33.0 41.0 36-37 37.73312627980007 40.0 37.0 41.0 33.0 41.0 38-39 37.62925054181022 40.0 37.0 41.0 33.0 41.0 40-41 37.53821834664019 40.0 37.0 41.0 33.0 41.0 42-43 37.425533888308024 40.0 37.0 41.0 32.0 41.0 44-45 37.267240632403755 40.0 36.0 41.0 32.0 41.0 46-47 37.05642733037972 40.0 36.0 41.0 31.0 41.0 48-49 36.852803073923496 39.5 36.0 41.0 31.0 41.0 50-51 36.690107477455626 39.0 35.5 41.0 31.0 41.0 52-53 36.53243419058269 39.0 35.0 41.0 30.5 41.0 54-55 36.361481782029 39.0 35.0 41.0 30.0 41.0 56-57 36.155436722968616 39.0 35.0 41.0 30.0 41.0 58-59 35.94845239492133 38.5 35.0 40.0 30.0 41.0 60-61 35.705947259564205 38.0 35.0 40.0 29.0 41.0 62-63 35.461129623058426 37.5 34.5 40.0 29.0 41.0 64-65 35.196687510363105 37.0 34.0 40.0 29.0 41.0 66-67 34.89347534473186 36.5 34.0 39.5 29.0 41.0 68-69 34.58988051395781 36.0 34.0 39.0 28.0 41.0 70-71 34.279653998812876 35.5 34.0 39.0 28.0 41.0 72-73 33.955138305755256 35.0 34.0 38.5 28.0 40.0 74-75 33.64264165073028 35.0 33.5 37.0 28.0 40.0 76-77 33.34893239505059 35.0 33.0 37.0 27.0 39.0 78-79 33.037937065796996 35.0 33.0 36.5 27.0 39.0 80-81 32.74140197521659 35.0 33.0 36.0 26.0 38.5 82-83 32.49808803752108 35.0 33.0 36.0 26.0 37.0 84-85 32.27771128780882 35.0 33.0 35.0 26.0 37.0 86-87 32.06334882843575 35.0 33.0 35.0 26.0 36.5 88-89 31.861185000697773 35.0 33.0 35.0 26.0 36.0 90-91 31.71089040054271 35.0 33.0 35.0 25.0 36.0 92-93 31.54566494266233 35.0 32.5 35.0 25.0 36.0 94-95 31.397336884880552 35.0 32.0 35.0 25.0 35.0 96-97 31.226907504195232 35.0 32.0 35.0 24.5 35.0 98-99 31.044594904085805 35.0 32.0 35.0 24.0 35.0 100 30.926675622953002 35.0 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3729053845670762 1101 2 0.40180228616820557 1101 3 0.42563367098687976 1101 4 0.30641903130299397 1101 5 0.30754570908109 1101 6 0.3335238615632079 1101 7 0.319863330482292 1101 8 0.3704181475218178 1101 9 0.48375488272746026 1101 10-11 0.4735780402090839 1101 12-13 0.5328256623099392 1101 14-15 0.5943988427187676 1101 16-17 0.59296005997615 1101 18-19 0.594246937392299 1101 20-21 0.5741579559726233 1101 22-23 0.6113311548981386 1101 24-25 0.6040948888184019 1101 26-27 0.5855018989218195 1101 28-29 0.5510427043387409 1101 30-31 0.6342576459936353 1101 32-33 0.6764676510598946 1101 34-35 0.6708640190658102 1101 36-37 0.6529064812261822 1101 38-39 0.6843025821822124 1101 40-41 0.6590360109236642 1101 42-43 0.5975896200895647 1101 44-45 0.6419551074767256 1101 46-47 0.6322774974431127 1101 48-49 0.6161797456017979 1101 50-51 0.5908089945374826 1101 52-53 0.6106277258056352 1101 54-55 0.6303666755635859 1101 56-57 0.5845096505359351 1101 58-59 0.5981047195426612 1101 60-61 0.5915785532826021 1101 62-63 0.6127381347421803 1101 64-65 0.5910032123266618 1101 66-67 0.5817315161344112 1101 68-69 0.6237188929054653 1101 70-71 0.5837921530445698 1101 72-73 0.49717798072617825 1101 74-75 0.4849060207417111 1101 76-77 0.4569110906548062 1101 78-79 0.49485861882345006 1101 80-81 0.4725900509742331 1101 82-83 0.46302396662532175 1101 84-85 0.4917350479536111 1101 86-87 0.4807479790434499 1101 88-89 0.45624641449634495 1101 90-91 0.4443671711206285 1101 92-93 0.4967218048576534 1101 94-95 0.5193150256740289 1101 96-97 0.5265258961213561 1101 98-99 0.5320147511352573 1101 100 0.548247322726926 1102 1 0.29226826268264006 1102 2 0.30500137946538075 1102 3 0.32995806847110387 1102 4 0.2739300079020026 1102 5 0.29504270400678223 1102 6 0.1750513371892879 1102 7 0.21068678937803043 1102 8 0.21470982578949815 1102 9 0.3300844146001154 1102 10-11 0.3860596254052808 1102 12-13 0.3621002638410431 1102 14-15 0.47923342737389163 1102 16-17 0.4402081239956601 1102 18-19 0.45386468615329534 1102 20-21 0.4312062756053976 1102 22-23 0.4005923477310489 1102 24-25 0.4144113164258627 1102 26-27 0.492346640150771 1102 28-29 0.4666037221910244 1102 30-31 0.3945900820168404 1102 32-33 0.4131581969995324 1102 34-35 0.4727243429749919 1102 36-37 0.5124713389156881 1102 38-39 0.522906005247826 1102 40-41 0.541048987950461 1102 42-43 0.5360593392222412 1102 44-45 0.5397833664059917 1102 46-47 0.5273781583315582 1102 48-49 0.5933677132057369 1102 50-51 0.5437942470252608 1102 52-53 0.5525675682565421 1102 54-55 0.5775091470355633 1102 56-57 0.5429834243659926 1102 58-59 0.512082692440984 1102 60-61 0.49899379221965745 1102 62-63 0.444826236490961 1102 64-65 0.47327580010593095 1102 66-67 0.41440399078251033 1102 68-69 0.45736080663334633 1102 70-71 0.42489636231130845 1102 72-73 0.45482134296339183 1102 74-75 0.4198786736078901 1102 76-77 0.45572445178436993 1102 78-79 0.3856554985296583 1102 80-81 0.4129906703759971 1102 82-83 0.46141061671054473 1102 84-85 0.3985301884741972 1102 86-87 0.3922938031562566 1102 88-89 0.42722488818573723 1102 90-91 0.3955507376076035 1102 92-93 0.3526211511837545 1102 94-95 0.3747124483254325 1102 96-97 0.4145584298870837 1102 98-99 0.39409368048703186 1102 100 0.41655937928814524 1103 1 0.3143721515259017 1103 2 0.29616413842237677 1103 3 0.2869418004423885 1103 4 0.25490249702727397 1103 5 0.27071005379467294 1103 6 0.2681197970898097 1103 7 0.24188217680368496 1103 8 0.2850344363931967 1103 9 0.35569803584699855 1103 10-11 0.3685651742481184 1103 12-13 0.3581718603198141 1103 14-15 0.45967400837584904 1103 16-17 0.39241810760459117 1103 18-19 0.3980292698205403 1103 20-21 0.4271804767025813 1103 22-23 0.4250909471351747 1103 24-25 0.4417743395168685 1103 26-27 0.4962492073656435 1103 28-29 0.49692812635756667 1103 30-31 0.4643862013698552 1103 32-33 0.5358804279147691 1103 34-35 0.5465149262661271 1103 36-37 0.5701153098859066 1103 38-39 0.5615747474522124 1103 40-41 0.6145677728765762 1103 42-43 0.5636602901038472 1103 44-45 0.5783671327032565 1103 46-47 0.6224166075680557 1103 48-49 0.6707931181006543 1103 50-51 0.6516292404362645 1103 52-53 0.6615430477865161 1103 54-55 0.6615919126720016 1103 56-57 0.66377492436051 1103 58-59 0.6506498693599312 1103 60-61 0.7136205036084604 1103 62-63 0.6429564689102776 1103 64-65 0.6046925338593923 1103 66-67 0.623114362157672 1103 68-69 0.5762083119089496 1103 70-71 0.5976454474707111 1103 72-73 0.6240445744128138 1103 74-75 0.5821612156547431 1103 76-77 0.5769359500678704 1103 78-79 0.5804657391971801 1103 80-81 0.5650985124485359 1103 82-83 0.5984495491957347 1103 84-85 0.5907105849741043 1103 86-87 0.5108180538120806 1103 88-89 0.5823893404973113 1103 90-91 0.5568000912513931 1103 92-93 0.5722593302830994 1103 94-95 0.595421451558412 1103 96-97 0.6016899155572304 1103 98-99 0.6293934026433092 1103 100 0.6377370534350355 1104 1 0.16124557266548578 1104 2 0.19685762769170267 1104 3 0.21507752317301154 1104 4 0.2150163989314393 1104 5 0.18594631078617851 1104 6 0.18498172767161236 1104 7 0.19510309732752518 1104 8 0.22175193544187266 1104 9 0.28604030933110636 1104 10-11 0.28838157766649175 1104 12-13 0.2547237685747632 1104 14-15 0.31323040153811377 1104 16-17 0.30588876277660404 1104 18-19 0.30520858564841546 1104 20-21 0.306013609175686 1104 22-23 0.33184196170132907 1104 24-25 0.31567799339580205 1104 26-27 0.34716707783870504 1104 28-29 0.3181874063454302 1104 30-31 0.3087453787172194 1104 32-33 0.31387041598485155 1104 34-35 0.4027573192203704 1104 36-37 0.43655881619346815 1104 38-39 0.4471596789930956 1104 40-41 0.4416398716496275 1104 42-43 0.4224029080808407 1104 44-45 0.43288222298392753 1104 46-47 0.4522023646488691 1104 48-49 0.4338738383126355 1104 50-51 0.42562411577854675 1104 52-53 0.41553249551710536 1104 54-55 0.35642275610975815 1104 56-57 0.37425753440132326 1104 58-59 0.31386894981534397 1104 60-61 0.4208446998488853 1104 62-63 0.3672071055216506 1104 64-65 0.3670185935160717 1104 66-67 0.4014879471064816 1104 68-69 0.3500083261012037 1104 70-71 0.35155253016107224 1104 72-73 0.3921351617424307 1104 74-75 0.35431182788043003 1104 76-77 0.33067965342474537 1104 78-79 0.39363414654510365 1104 80-81 0.3439066919698561 1104 82-83 0.38727416621394184 1104 84-85 0.33649947737522723 1104 86-87 0.3827294754578574 1104 88-89 0.4326054839251263 1104 90-91 0.4562488226654402 1104 92-93 0.40476891061501874 1104 94-95 0.4441045251491218 1104 96-97 0.4155965684505851 1104 98-99 0.44559680362781506 1104 100 0.4444692048578105 1105 1 0.18904351486013837 1105 2 0.143348766610643 1105 3 0.1775106254819896 1105 4 0.19392025580785344 1105 5 0.1556006573816262 1105 6 0.14249949971336662 1105 7 0.19603187201738592 1105 8 0.14621654959398 1105 9 0.2450104616114288 1105 10-11 0.2071682882330208 1105 12-13 0.26791676709662937 1105 14-15 0.27497403704891354 1105 16-17 0.25283054860322096 1105 18-19 0.26172074253170763 1105 20-21 0.30421536919187275 1105 22-23 0.2991189489330708 1105 24-25 0.27532033772547493 1105 26-27 0.2863211066961 1105 28-29 0.2902243841961223 1105 30-31 0.2794361239799059 1105 32-33 0.30172375360878334 1105 34-35 0.31119313534826887 1105 36-37 0.33744155462657943 1105 38-39 0.35453464710173677 1105 40-41 0.3808360534574149 1105 42-43 0.3226544539576679 1105 44-45 0.33889781944248654 1105 46-47 0.3216628894989668 1105 48-49 0.29618771404649635 1105 50-51 0.278683808250463 1105 52-53 0.2892495170012168 1105 54-55 0.29099297509738165 1105 56-57 0.26528969544375514 1105 58-59 0.2326300551078475 1105 60-61 0.22413235344565408 1105 62-63 0.17999766031501707 1105 64-65 0.21656431765416784 1105 66-67 0.22274002090200185 1105 68-69 0.21124250168940506 1105 70-71 0.26860402930019944 1105 72-73 0.2631094194238699 1105 74-75 0.20102291090282876 1105 76-77 0.18202999360249095 1105 78-79 0.24095456805075344 1105 80-81 0.21284599951945182 1105 82-83 0.23175750659799377 1105 84-85 0.23160512475063655 1105 86-87 0.21365770072740276 1105 88-89 0.277198286917244 1105 90-91 0.22112054278117554 1105 92-93 0.22141737598785127 1105 94-95 0.26981910848074975 1105 96-97 0.20521280984361212 1105 98-99 0.21069636360750366 1105 100 0.21822685465832592 1106 1 0.12336946544265004 1106 2 0.1570131931656178 1106 3 0.19230590080171694 1106 4 0.16362120376299316 1106 5 0.10911682382887022 1106 6 0.0938489838889538 1106 7 0.14080066344623532 1106 8 0.15267210309010437 1106 9 0.16220869143275252 1106 10-11 0.15292434026068236 1106 12-13 0.21264005725191737 1106 14-15 0.25628182247206865 1106 16-17 0.23521193000755858 1106 18-19 0.2658418967783973 1106 20-21 0.2215337558146402 1106 22-23 0.22665230697961647 1106 24-25 0.23788724967705122 1106 26-27 0.21946424390978336 1106 28-29 0.23250332682772523 1106 30-31 0.2446189459474013 1106 32-33 0.24599782079684474 1106 34-35 0.1825258855646652 1106 36-37 0.14713969368420976 1106 38-39 0.2035599739943521 1106 40-41 0.19998276484987798 1106 42-43 0.22743962106796545 1106 44-45 0.2324504920033732 1106 46-47 0.21096897238628287 1106 48-49 0.16164662006690378 1106 50-51 0.22033532534174327 1106 52-53 0.18364148622945464 1106 54-55 0.15499975753393613 1106 56-57 0.14503441538521145 1106 58-59 0.14117351754219243 1106 60-61 0.17902992422700947 1106 62-63 0.07763971027517158 1106 64-65 0.049144509767472755 1106 66-67 0.05433915300999814 1106 68-69 0.10238054467127 1106 70-71 0.10827578956175188 1106 72-73 0.12124150257487543 1106 74-75 0.1277171776945849 1106 76-77 0.07901033800771273 1106 78-79 0.05456640420986503 1106 80-81 0.038937915435958814 1106 82-83 0.04055972199648039 1106 84-85 0.005151783022455447 1106 86-87 0.01906993032324067 1106 88-89 0.09906540122409169 1106 90-91 0.13874014544637703 1106 92-93 0.16935185224311056 1106 94-95 0.11353000127067148 1106 96-97 0.1716520402056929 1106 98-99 0.1833065841728363 1106 100 0.17640073687953262 1107 1 0.13314719868979097 1107 2 0.14577548016921327 1107 3 0.17809169098984157 1107 4 0.1718249336748343 1107 5 0.14571334189701446 1107 6 0.024025725023477662 1107 7 -0.07698936186236693 1107 8 -0.029310840819597672 1107 9 -0.06346298846304421 1107 10-11 -0.05391436292153173 1107 12-13 0.15434715233114105 1107 14-15 -0.08968040778707831 1107 16-17 0.012869285061135827 1107 18-19 0.06056126465101386 1107 20-21 0.14202767577702247 1107 22-23 -0.0157493560469959 1107 24-25 0.1570431446930698 1107 26-27 -0.022306202155533583 1107 28-29 0.037307784879708095 1107 30-31 -9.245939775581746E-5 1107 32-33 0.08578465579574868 1107 34-35 0.18447095568597405 1107 36-37 0.09232735499402622 1107 38-39 0.14495038398984406 1107 40-41 0.16809468966204122 1107 42-43 0.07199686985816811 1107 44-45 0.15469814539402904 1107 46-47 0.27870016506981443 1107 48-49 0.183977542804854 1107 50-51 0.25661629032492783 1107 52-53 0.2343040571479733 1107 54-55 0.2152193532514204 1107 56-57 0.1688833773664271 1107 58-59 0.08900411657667462 1107 60-61 0.11733552921050006 1107 62-63 0.10539494950263872 1107 64-65 0.16863996587626673 1107 66-67 0.0821190336828792 1107 68-69 0.1251546641929977 1107 70-71 0.16344309897892373 1107 72-73 0.08103939021701478 1107 74-75 0.1054909624336986 1107 76-77 0.17233026638832172 1107 78-79 0.18203230455350194 1107 80-81 0.17621640254611037 1107 82-83 0.2296551779125977 1107 84-85 0.1342485708434964 1107 86-87 0.1447237005535058 1107 88-89 0.2304300329583917 1107 90-91 0.14518696029087863 1107 92-93 0.17916586243784494 1107 94-95 0.17176146033519046 1107 96-97 0.2306267359388734 1107 98-99 0.3567471812130698 1107 100 0.24784229154255044 1108 1 0.03158803880971206 1108 2 0.08439661829043033 1108 3 0.07374436691430475 1108 4 0.06232920698447231 1108 5 0.06602866277673769 1108 6 0.0836774784323211 1108 7 0.06386877269444113 1108 8 0.08070736531198719 1108 9 0.14635248352802677 1108 10-11 0.13114986327055078 1108 12-13 0.1311738910633764 1108 14-15 0.18486439082550277 1108 16-17 0.1755018313565273 1108 18-19 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-0.6321405394027764 2215 22-23 -0.6493968848178682 2215 24-25 -0.6786530628792278 2215 26-27 -0.6315988138935325 2215 28-29 -0.6086644201129019 2215 30-31 -0.6833994943331945 2215 32-33 -0.6662156534229311 2215 34-35 -0.6461693831764492 2215 36-37 -0.662616996782603 2215 38-39 -0.6669676778991018 2215 40-41 -0.6657958316057204 2215 42-43 -0.6716683735411095 2215 44-45 -0.6104267002414971 2215 46-47 -0.7015385753852001 2215 48-49 -0.7012438610946106 2215 50-51 -0.7187191606046497 2215 52-53 -0.5967504188153754 2215 54-55 -0.5868745627501752 2215 56-57 -0.586560970518903 2215 58-59 -0.7464096328809688 2215 60-61 -0.6286720391454779 2215 62-63 -0.5941240865196207 2215 64-65 -0.6475836284674017 2215 66-67 -0.6166887439976207 2215 68-69 -0.6814650508490843 2215 70-71 -0.5631760637473278 2215 72-73 -0.5340238672341968 2215 74-75 -0.5361522767954838 2215 76-77 -0.5025129335605456 2215 78-79 -0.5481788170552591 2215 80-81 -0.565548122178356 2215 82-83 -0.5139239438010534 2215 84-85 -0.5625711915530935 2215 86-87 -0.5476923652463768 2215 88-89 -0.507114030285539 2215 90-91 -0.4664735600057064 2215 92-93 -0.543243791863798 2215 94-95 -0.620939062781865 2215 96-97 -0.6884325103032971 2215 98-99 -0.6335670796970945 2215 100 -0.6280511751300324 2216 1 -0.3882780806927322 2216 2 -0.40727041164139877 2216 3 -0.4358101021364078 2216 4 -0.3249366538288072 2216 5 -0.3944951770495351 2216 6 -0.3435567197087366 2216 7 -0.38485238788765486 2216 8 -0.3497687368502298 2216 9 -0.48915980853317365 2216 10-11 -0.46768899337555325 2216 12-13 -0.5384057222220946 2216 14-15 -0.6511321919307989 2216 16-17 -0.6703657750743375 2216 18-19 -0.6457447854875937 2216 20-21 -0.6863556593621425 2216 22-23 -0.6760062041178685 2216 24-25 -0.7439658894977015 2216 26-27 -0.9073925679214625 2216 28-29 -0.7146140921655828 2216 30-31 -0.6381209504609799 2216 32-33 -0.768400185367156 2216 34-35 -0.8835426870971403 2216 36-37 -0.831592558297892 2216 38-39 -0.7308760460576025 2216 40-41 -0.8478013927487353 2216 42-43 -0.8160321752018334 2216 44-45 -0.703320993037174 2216 46-47 -0.9014716971730721 2216 48-49 -0.8974753310602637 2216 50-51 -0.7915060007522925 2216 52-53 -0.8628366254950279 2216 54-55 -0.9314361565031888 2216 56-57 -0.9370366789149784 2216 58-59 -0.8261546816445247 2216 60-61 -0.9103468600403914 2216 62-63 -0.9342305237904256 2216 64-65 -0.7702275795575915 2216 66-67 -0.8395513261012937 2216 68-69 -0.8217901543450594 2216 70-71 -0.8385600464787402 2216 72-73 -0.9467429283905346 2216 74-75 -0.9105136430873486 2216 76-77 -0.8093030607519083 2216 78-79 -0.7306947140951152 2216 80-81 -0.7716686029613982 2216 82-83 -0.726881799467737 2216 84-85 -0.7858917625677044 2216 86-87 -0.8431628176567934 2216 88-89 -0.906228297708946 2216 90-91 -0.7847439561158289 2216 92-93 -0.9430332163257624 2216 94-95 -0.935299121759531 2216 96-97 -1.0259509456261817 2216 98-99 -1.0159737644644942 2216 100 -1.0055354346221677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 846.0 3 116.0 4 139.0 5 525.0 6 1395.0 7 2747.0 8 4592.0 9 6416.0 10 8975.0 11 12893.0 12 18147.0 13 23206.0 14 24382.0 15 29290.0 16 36673.0 17 47393.0 18 47304.0 19 31715.0 20 26923.0 21 27391.0 22 29777.0 23 33621.0 24 40575.0 25 51487.0 26 67038.0 27 88148.0 28 116106.0 29 152168.0 30 195211.0 31 250514.0 32 318494.0 33 410075.0 34 553433.0 35 795635.0 36 1264017.0 37 2164951.0 38 2371274.0 39 648294.0 40 1028.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.72242053265843 19.017962405518567 13.394170330446498 23.865446731376505 2 16.761945019415496 20.39240167086173 37.88929198011817 24.956361329604597 3 18.64923799196883 25.57769359604658 29.56241970797687 26.210648704007728 4 13.446368703800946 17.20103405097548 36.52183220907218 32.83076503615139 5 14.031930399476355 35.98364077482648 33.96050899765463 16.023919828042533 6 31.061998518819816 36.15491359411987 18.056967878343688 14.726120008716626 7 27.45811357046702 31.377073140831648 22.610144150572324 18.55466913812901 8 26.07271960556257 33.59569718569706 20.748377699735656 19.583205509004724 9 25.628708883062096 16.332778412495554 21.35192732159443 36.686585382847916 10-11 24.617874870694546 25.78911669178387 28.329885524534816 21.26312291298677 12-13 25.34094620131796 23.563216243513757 28.390098963109935 22.705738592058353 14-15 22.84193806050557 25.127326467324185 26.18764320314575 25.8430922690245 16-17 22.320253412278447 27.908229840226827 26.636689968225514 23.13482677926921 18-19 22.258536683883932 26.74611349258042 29.011526984655934 21.983822838879714 20-21 23.10667849887417 26.1081132280862 28.778448444568944 22.006759828470692 22-23 22.914495331020362 25.97105597171034 28.653839725917145 22.46060897135215 24-25 22.72698562101641 26.040391944150045 28.76463190217038 22.467990532663165 26-27 22.650493603295725 25.935542330460713 28.773841436300035 22.640122629943523 28-29 22.59871544664628 25.928337883973345 28.90586867709747 22.567077992282904 30-31 22.76788504791404 26.234582830236864 28.618803153317952 22.378728968531146 32-33 22.603346853259556 26.117363030720046 28.696225171701983 22.58306494431841 34-35 22.746740658591072 25.796983857982763 28.735517158766655 22.720758324659514 36-37 23.020661850743405 25.941438945360773 28.522053486789872 22.515845717105954 38-39 22.882118334058035 26.060682744493185 28.484232014940247 22.57296690650853 40-41 22.80869515385561 25.85885055068618 28.589618820195856 22.742835475262353 42-43 22.723913519293966 26.042607205311775 28.450073097256084 22.783406178138176 44-45 22.789830154699178 26.207861371828322 28.24325729186742 22.75905118160508 46-47 22.932714552504443 26.163339396868434 28.02269109880183 22.88125495182529 48-49 22.957798078424187 26.169160915665834 27.967182184960908 22.905858820949064 50-51 22.88623328446556 26.231162867818504 27.804972354601887 23.077631493114048 52-53 22.823201332456286 26.295744868631598 27.736108785757402 23.144945013154715 54-55 22.861982846665132 26.57079017347823 27.67383923560277 22.89338774425386 56-57 22.68854674982702 26.328564175208697 27.722725985671115 23.260163089293165 58-59 22.65330689532394 26.013065447200695 27.912996114073092 23.420631543402276 60-61 22.860764446823094 26.267999439558814 27.780307056131214 23.090929057486875 62-63 22.66335235484558 26.35180334104807 27.790127962885254 23.1947163412211 64-65 22.686112405484188 26.071567978552558 27.848455400442283 23.393864215520967 66-67 22.56782073296196 26.183497591167843 27.8043293946191 23.4443522812511 68-69 22.797961391864046 26.208318123042545 27.658084780772374 23.335635704321035 70-71 22.795511935111673 25.97791048550117 27.714091512318035 23.512486067069123 72-73 22.5867621513665 26.187109444345367 27.783811938307228 23.442316465980902 74-75 22.60417813763874 26.16632804735793 27.674066305159688 23.55542750984364 76-77 22.727618362093594 26.18079848106486 27.477421118445456 23.614162038396092 78-79 22.64709940974983 26.363810834567726 27.56727973897533 23.421810016707116 80-81 22.52556326997995 26.19604980786366 27.627932226995704 23.650454695160686 82-83 22.54220282317529 26.12629724730917 27.635482644867782 23.69601728464776 84-85 22.552033105687972 26.221756810931822 27.404135989409088 23.822074093971118 86-87 22.626240308388628 26.281362311650426 27.404096980565 23.68830039939595 88-89 22.45608061010066 26.127759631561197 27.483699331825722 23.932460426512417 90-91 22.263835969162372 26.105502638353634 27.5221793276639 24.108482064820098 92-93 22.403980172251696 26.17490698800281 27.401275800844356 24.01983703890114 94-95 22.353573920131122 25.942694422748662 27.532985008106614 24.1707466490136 96-97 22.270407818073235 26.032799012965828 27.464046020124815 24.23274714883612 98-99 22.211830756703737 26.13626030336695 27.391368470511868 24.260540469417442 100 22.284372398172454 25.960347958854456 27.37506557425884 24.380214068714253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5047.0 1 3518.5 2 10070.0 3 15063.5 4 7655.5 5 3145.0 6 4540.0 7 9335.0 8 14910.5 9 16627.5 10 12046.0 11 6829.5 12 5465.5 13 5713.5 14 6351.0 15 7034.0 16 7659.5 17 8338.0 18 9342.5 19 10348.5 20 11513.0 21 13470.5 22 16107.0 23 20244.5 24 25922.0 25 32696.0 26 41769.0 27 53753.5 28 69154.5 29 87335.0 30 105735.0 31 124655.0 32 144193.5 33 160741.0 34 176461.0 35 194750.5 36 214892.0 37 237808.0 38 260012.0 39 276162.0 40 291473.5 41 307343.0 42 317393.5 43 325424.5 44 333773.0 45 340006.0 46 345700.0 47 346233.0 48 343883.0 49 344923.0 50 346068.0 51 345656.5 52 339076.5 53 335297.5 54 332427.5 55 317621.5 56 298412.0 57 273119.5 58 247497.0 59 229694.5 60 209784.5 61 183655.5 62 156147.5 63 128551.0 64 102919.0 65 82351.5 66 63934.0 67 48126.0 68 36107.0 69 26793.0 70 19581.0 71 14426.0 72 10910.0 73 8082.0 74 6273.0 75 4452.5 76 2548.0 77 1496.0 78 932.5 79 489.5 80 216.0 81 92.5 82 39.5 83 25.0 84 17.0 85 14.5 86 10.5 87 5.5 88 3.5 89 2.0 90 2.0 91 2.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13669713783235926 2 0.0 3 0.0 4 0.0011511763103264352 5 0.0 6 0.0 7 5.049018904940505E-5 8 0.0 9 0.0 10-11 6.0588226859286066E-5 12-13 3.231372099161923E-4 14-15 2.4235290743714427E-4 16-17 0.0 18-19 6.462744198323846E-4 20-21 0.0 22-23 0.0021710781291244177 24-25 2.3730388853220375E-4 26-27 0.0027820094166222183 28-29 9.542645730337556E-4 30-31 7.573528357410758E-4 32-33 0.0 34-35 3.1808819101125185E-4 36-37 1.5147056714821517E-5 38-39 0.0 40-41 4.1906856911006195E-4 42-43 1.1612743481363163E-4 44-45 4.998528715891101E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011915684615659591 58-59 0.0 60-61 1.5147056714821517E-5 62-63 3.534313233458354E-5 64-65 7.573528357410759E-5 66-67 0.0019186271838773918 68-69 7.977449869805999E-4 70-71 0.0011713723859461973 72-73 3.6352936115571636E-4 74-75 0.0020246565808811428 76-77 0.001888333070447749 78-79 0.0025093623957554313 80-81 0.0018226958246835225 82-83 2.2215683181738222E-4 84-85 8.482351760300049E-4 86-87 8.532841949349455E-4 88-89 0.004872303243267588 90-91 0.0034030387419299005 92-93 0.004321960182629073 94-95 0.0019741663918317375 96-97 0.0011713723859461973 98-99 2.2720585072232274E-4 100 1.9186271838773922E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9902914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.65576416255734 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3079778555889 36.6281587332413 2 15.514671200110769 15.718150507448634 3 5.12175211141511 7.783388011647718 4 2.177123687085165 4.411354561828137 5 1.209443505810532 3.063264249913743 6 0.7705396362709037 2.3419364455704974 7 0.5166818551667921 1.832103994168114 8 0.37110246448823114 1.5038783137007719 9 0.29268421375580383 1.334352825550577 >10 1.5393005373914541 14.372872686257454 >50 0.12145550206922903 4.220113541260764 >100 0.05531202373516042 4.771179956615278 >500 0.0013972072472557416 0.470378978212865 >1k 3.9871462721151096E-4 0.27050080809199073 >5k 5.9806964045233585E-5 0.19964849600636192 >10k+ 9.967827340872264E-5 1.078717890485765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30641 0.3094139765325641 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26314 0.2657197669292089 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22959 0.23184085007705812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15546 0.15698409579241018 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11359 0.1147036114824384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.009803780988101E-5 0.0 0.0 0.0 3 0.0 1.009803780988101E-5 0.0 0.0 0.0 4 0.0 2.019607561976202E-5 0.0 0.0 0.0 5 0.0 2.019607561976202E-5 0.0 0.0 0.0 6 0.0 2.019607561976202E-5 0.0 0.0 0.0 7 0.0 2.019607561976202E-5 0.0 0.0 0.0 8 0.0 2.019607561976202E-5 0.0 1.009803780988101E-5 0.0 9 0.0 2.019607561976202E-5 0.0 3.0294113429643033E-5 0.0 10-11 0.0 2.019607561976202E-5 0.0 4.039215123952404E-5 0.0 12-13 0.0 2.019607561976202E-5 0.0 5.553920795434556E-5 0.0 14-15 0.0 2.5245094524702525E-5 0.0 1.2622547262351264E-4 5.049018904940505E-6 16-17 0.0 4.039215123952404E-5 0.0 1.4642154824327466E-4 1.009803780988101E-5 18-19 0.0 4.039215123952404E-5 0.0 1.6156860495809616E-4 1.009803780988101E-5 20-21 0.0 4.039215123952404E-5 0.0 2.776960397717278E-4 1.009803780988101E-5 22-23 0.0 4.039215123952404E-5 0.0 7.573528357410757E-4 1.009803780988101E-5 24-25 0.0 4.039215123952404E-5 0.0 0.0016308331062957833 2.019607561976202E-5 26-27 0.0 4.039215123952404E-5 2.019607561976202E-5 0.003160685834492756 2.019607561976202E-5 28-29 0.0 7.573528357410759E-5 2.019607561976202E-5 0.007119116655966113 2.019607561976202E-5 30-31 1.009803780988101E-5 9.08823402889291E-5 2.019607561976202E-5 0.017398919146424982 2.019607561976202E-5 32-33 1.009803780988101E-5 1.0098037809881011E-4 2.019607561976202E-5 0.04261371955769787 2.019607561976202E-5 34-35 2.019607561976202E-5 1.0602939700375062E-4 2.019607561976202E-5 0.08758028192509801 2.019607561976202E-5 36-37 2.019607561976202E-5 1.2622547262351264E-4 2.019607561976202E-5 0.15556027246121695 2.019607561976202E-5 38-39 2.019607561976202E-5 1.5651958605315564E-4 2.019607561976202E-5 0.23907609416783787 2.019607561976202E-5 40-41 2.019607561976202E-5 1.7671566167291771E-4 2.019607561976202E-5 0.32468725872000903 2.019607561976202E-5 42-43 2.5245094524702525E-5 2.1205879400750124E-4 2.019607561976202E-5 0.41549386372536407 2.019607561976202E-5 44-45 3.0294113429643033E-5 2.2215683181738225E-4 2.019607561976202E-5 0.533201641456242 2.019607561976202E-5 46-47 3.0294113429643033E-5 2.2215683181738225E-4 2.019607561976202E-5 0.6755940726133742 2.019607561976202E-5 48-49 3.0294113429643033E-5 2.2720585072232274E-4 2.019607561976202E-5 0.8184207193963312 2.019607561976202E-5 50-51 3.0294113429643033E-5 2.5749996415196574E-4 2.019607561976202E-5 0.9616664347483983 2.019607561976202E-5 52-53 3.0294113429643033E-5 2.675980019618468E-4 2.5245094524702525E-5 1.1069216596246316 3.0294113429643033E-5 54-55 3.0294113429643033E-5 2.877940775816088E-4 3.0294113429643033E-5 1.273150509031988 3.0294113429643033E-5 56-57 3.0294113429643033E-5 3.029411342964303E-4 3.0294113429643033E-5 1.4730563145352975 3.0294113429643033E-5 58-59 3.0294113429643033E-5 3.029411342964303E-4 3.0294113429643033E-5 1.6920070193480425 3.0294113429643033E-5 60-61 3.0294113429643033E-5 3.1303917210631134E-4 3.0294113429643033E-5 1.9164460077104577 3.0294113429643033E-5 62-63 3.0294113429643033E-5 3.1303917210631134E-4 3.0294113429643033E-5 2.1447727406296773 3.0294113429643033E-5 64-65 3.0294113429643033E-5 3.1303917210631134E-4 3.0294113429643033E-5 2.395865499791274 3.0294113429643033E-5 66-67 3.0294113429643033E-5 3.433332855359544E-4 3.0294113429643033E-5 2.686047763314919 3.0294113429643033E-5 68-69 3.0294113429643033E-5 3.635293611557164E-4 3.0294113429643033E-5 3.002696983938263 3.0294113429643033E-5 70-71 3.0294113429643033E-5 3.635293611557164E-4 3.0294113429643033E-5 3.33948169195451 3.0294113429643033E-5 72-73 3.0294113429643033E-5 3.635293611557164E-4 3.0294113429643033E-5 3.690883309700559 3.0294113429643033E-5 74-75 4.039215123952404E-5 3.6857838006065693E-4 3.0294113429643033E-5 4.049106151987183 3.0294113429643033E-5 76-77 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 4.435825656973291 3.0294113429643033E-5 78-79 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 4.868178194822251 3.0294113429643033E-5 80-81 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 5.329390924731852 3.0294113429643033E-5 82-83 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 5.807861201258539 3.0294113429643033E-5 84-85 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 6.284438095695873 3.0294113429643033E-5 86-87 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 6.786118712128571 3.0294113429643033E-5 88 4.039215123952404E-5 3.938234745853594E-4 3.0294113429643033E-5 7.188298313001607 3.0294113429643033E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16295 0.0 48.430733 1 GTATCAA 27675 0.0 36.779953 1 TACCTGG 9210 0.0 35.261536 2 TATAACG 1285 0.0 35.11162 2 GTACCTG 11040 0.0 30.605373 1 ATCAACG 32735 0.0 30.5091 3 TCAACGC 33135 0.0 30.2259 4 CAACGCA 33770 0.0 29.741049 5 TATCAAC 34685 0.0 28.997122 2 ATAACGC 1665 0.0 28.791796 3 AACGCAG 35750 0.0 28.317623 6 TAACGCA 1645 0.0 27.141916 4 ACCTGGG 12170 0.0 26.299006 3 ACGCAGA 40895 0.0 24.628567 7 CGCAGAG 42200 0.0 23.800125 8 GTATAAC 2850 0.0 22.456205 1 GCAGAGT 46890 0.0 21.299326 9 GTACATG 29900 0.0 21.200138 1 GTGGTAT 8185 0.0 21.157845 1 TATCACG 1810 0.0 20.77276 2 >>END_MODULE