FastQCFastQC Report
Wed 25 May 2016
SRR2049537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830575
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155280.2663202171312435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8000.045.2364042
TACCTGG62650.042.5352822
GTACCTG76450.035.4220851
TAACGCA11400.034.6334154
GTATAAC14900.031.8686891
ATAACGC12550.031.0861173
ACCTGGG86800.030.595733
GGTATCA67000.028.9803981
GTATCAA94500.027.7607691
GCTACGC12800.026.0696812
ATCAACG100250.025.0373883
TCAACGC101300.024.8700264
CAACGCA104900.023.882315
AACGCAG112150.022.8368436
GTACATG202100.022.5882191
TACGCCC16650.022.0191864
TATCAAC116750.021.8992562
GGCTACG19750.021.1861481
TACATGG206800.021.1131252
ACATGGG202750.020.517033
Produced by FastQC (version 0.11.5)