##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049537_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5830575
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.095653344652973	31.0	30.0	34.0	26.0	34.0
2	30.258498175565876	31.0	31.0	34.0	26.0	34.0
3	30.34153526881997	31.0	31.0	34.0	26.0	34.0
4	33.98094750517745	37.0	35.0	37.0	30.0	37.0
5	33.917698854744174	37.0	35.0	37.0	30.0	37.0
6	34.00139711777998	37.0	35.0	37.0	30.0	37.0
7	33.906108574197226	37.0	35.0	37.0	30.0	37.0
8	33.88403202085558	37.0	35.0	37.0	30.0	37.0
9	35.33878305312941	39.0	35.0	39.0	29.0	39.0
10-11	35.26127800431347	39.0	35.0	39.0	29.0	39.0
12-13	35.17648722467338	39.0	35.0	39.0	29.0	39.0
14-15	36.23120275444531	40.0	36.0	41.0	28.0	41.0
16-17	36.193301861308704	40.0	36.0	41.0	28.0	41.0
18-19	36.17045197086051	40.0	36.0	41.0	27.5	41.0
20-21	36.15904983642265	40.0	36.0	41.0	27.0	41.0
22-23	36.07832469353366	40.0	36.0	41.0	27.0	41.0
24-25	36.05124580337274	40.0	36.0	41.0	27.0	41.0
26-27	36.00787023578292	40.0	36.0	41.0	27.0	41.0
28-29	35.92533995360663	40.0	36.0	41.0	27.0	41.0
30-31	35.8462116515095	39.0	36.0	41.0	27.0	41.0
32-33	35.79800808668099	39.0	35.5	41.0	27.0	41.0
34-35	35.73040815013957	39.0	35.0	41.0	27.0	41.0
36-37	35.61292676965823	39.0	35.0	41.0	27.0	41.0
38-39	35.505330606329565	39.0	35.0	41.0	26.5	41.0
40-41	35.42544680413167	39.0	35.0	41.0	26.0	41.0
42-43	35.216208521458	39.0	35.0	41.0	26.0	41.0
44-45	35.06342264699451	39.0	35.0	40.5	25.0	41.0
46-47	34.89941943976366	39.0	34.0	40.0	25.0	41.0
48-49	34.7236083919682	38.0	34.0	40.0	24.0	41.0
50-51	34.53815558499805	38.0	34.0	40.0	24.0	41.0
52-53	34.36055406199217	38.0	34.0	40.0	24.0	41.0
54-55	34.13739236696209	38.0	33.0	40.0	23.0	41.0
56-57	33.90786543351213	37.5	33.0	40.0	23.0	41.0
58-59	33.67055813534686	37.0	33.0	40.0	22.0	41.0
60-61	33.385422878532566	37.0	33.0	40.0	22.0	41.0
62-63	33.064882451559235	36.0	32.5	39.5	20.5	41.0
64-65	32.732638204636764	36.0	32.0	39.0	20.0	41.0
66-67	32.44350359955922	35.0	32.0	39.0	19.5	40.5
68-69	32.116465014170984	35.0	32.0	38.5	18.0	40.0
70-71	31.782789004514992	35.0	31.5	37.5	17.5	40.0
72-73	31.43284204388075	35.0	31.0	37.0	14.0	39.5
74-75	31.10675164970865	35.0	31.0	36.5	11.0	39.0
76-77	30.817198475279024	35.0	31.0	36.0	7.5	39.0
78-79	30.50297294863714	35.0	31.0	35.5	7.0	37.5
80-81	30.184110915304238	34.0	30.5	35.0	2.0	37.0
82-83	29.89952843415958	34.0	30.0	35.0	2.0	37.0
84-85	29.610558478366197	34.0	30.0	35.0	2.0	36.0
86-87	29.354421047666825	34.0	30.0	35.0	2.0	36.0
88-89	29.0560408707546	34.0	29.5	35.0	2.0	36.0
90-91	28.80017056636781	34.0	29.0	35.0	2.0	35.0
92-93	28.566812021112842	34.0	29.0	35.0	2.0	35.0
94-95	28.31742581134794	34.0	29.0	35.0	2.0	35.0
96-97	28.04345326147078	34.0	29.0	35.0	2.0	35.0
98-99	27.746733040909344	34.0	29.0	35.0	2.0	35.0
100	27.52141426874708	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4262109563092835
1101	2	0.5237312522196191
1101	3	0.5250090181289906
1101	4	0.516502043398468
1101	5	0.45118078858666166
1101	6	0.4578418184303672
1101	7	0.43645091066565556
1101	8	0.49320273992466923
1101	9	0.6262003616154814
1101	10-11	0.7102315160934296
1101	12-13	0.6264998836923894
1101	14-15	0.819997030319513
1101	16-17	0.7563874907158095
1101	18-19	0.7548714162008636
1101	20-21	0.7867346435031024
1101	22-23	0.8621339926170535
1101	24-25	0.8134749580082499
1101	26-27	0.7976558109458978
1101	28-29	0.8034734738483351
1101	30-31	0.8655225519535463
1101	32-33	0.8930499156244593
1101	34-35	0.8311328820233754
1101	36-37	0.8711813803399551
1101	38-39	0.8897176250144909
1101	40-41	0.8095213393340828
1101	42-43	0.7766295604411866
1101	44-45	0.8189155926210105
1101	46-47	0.7458614825927157
1101	48-49	0.7610903867203973
1101	50-51	0.774808362762748
1101	52-53	0.8092835148714101
1101	54-55	0.7983878647061431
1101	56-57	0.7996136785020482
1101	58-59	0.7617060289117887
1101	60-61	0.7667220791136842
1101	62-63	0.799820674609812
1101	64-65	0.6731124376029385
1101	66-67	0.7817381999040194
1101	68-69	0.745468691063877
1101	70-71	0.765426582305679
1101	72-73	0.7874052982241082
1101	74-75	0.7947789290547611
1101	76-77	0.6797686323803269
1101	78-79	0.7488831994844141
1101	80-81	0.7884123093150599
1101	82-83	0.7852247220436972
1101	84-85	0.7494064132485718
1101	86-87	0.7975398751523137
1101	88-89	0.7855036074591695
1101	90-91	0.6757385052948202
1101	92-93	0.723820575466533
1101	94-95	0.7156971500833187
1101	96-97	0.7813952776697093
1101	98-99	0.6891596348083482
1101	100	0.6508460786692538
1102	1	0.29704099625772784
1102	2	0.36914208799479553
1102	3	0.3694810551804082
1102	4	0.35701377401352374
1102	5	0.2685352081355603
1102	6	0.4030828462965559
1102	7	0.36707258039838564
1102	8	0.3778697314613666
1102	9	0.4230199278718132
1102	10-11	0.5214940019023828
1102	12-13	0.5312321063335474
1102	14-15	0.6185935402337108
1102	16-17	0.6332078795192473
1102	18-19	0.6385067143516707
1102	20-21	0.5985932531320728
1102	22-23	0.6508920641190841
1102	24-25	0.5691158874871078
1102	26-27	0.6621491186705555
1102	28-29	0.6884067665997051
1102	30-31	0.6140570835487011
1102	32-33	0.6656475946442058
1102	34-35	0.6919464533629096
1102	36-37	0.6751881985678168
1102	38-39	0.7341446034197148
1102	40-41	0.6572663196202484
1102	42-43	0.6922793291181151
1102	44-45	0.7282999414246518
1102	46-47	0.6610856213450802
1102	48-49	0.6602204874574227
1102	50-51	0.7416581469611216
1102	52-53	0.743621193915665
1102	54-55	0.6743139484083684
1102	56-57	0.6713932685396742
1102	58-59	0.7263108309220101
1102	60-61	0.6473452760018716
1102	62-63	0.5753245408119625
1102	64-65	0.5600763457546662
1102	66-67	0.5669720199784294
1102	68-69	0.6015258694831687
1102	70-71	0.6197921674698748
1102	72-73	0.618710605185619
1102	74-75	0.6165571495069031
1102	76-77	0.5372552251252394
1102	78-79	0.5892482149456093
1102	80-81	0.6490689473266436
1102	82-83	0.5963494057478371
1102	84-85	0.5517752279287578
1102	86-87	0.5808466781054413
1102	88-89	0.5487963338429367
1102	90-91	0.5287069779781319
1102	92-93	0.5463931316250665
1102	94-95	0.57944143540632
1102	96-97	0.6171237409300154
1102	98-99	0.5191582489883828
1102	100	0.517865455908467
1103	1	0.3155921911893351
1103	2	0.376472410046631
1103	3	0.351343662731157
1103	4	0.3694354815859242
1103	5	0.31027693934954925
1103	6	0.31368988524437214
1103	7	0.3492651786695049
1103	8	0.3771388886308742
1103	9	0.48147624746226114
1103	10-11	0.4833860918134931
1103	12-13	0.4322104140182006
1103	14-15	0.5721188745669963
1103	16-17	0.604781401090662
1103	18-19	0.5890471859484663
1103	20-21	0.5984141799554763
1103	22-23	0.572983653143325
1103	24-25	0.5517189668509914
1103	26-27	0.6227206134173002
1103	28-29	0.6334841769926882
1103	30-31	0.5804469367705565
1103	32-33	0.5996684419096567
1103	34-35	0.648743348820453
1103	36-37	0.6189169038708116
1103	38-39	0.6126183484216128
1103	40-41	0.5771984340736935
1103	42-43	0.6886088965289119
1103	44-45	0.6429469251709747
1103	46-47	0.5601491555513363
1103	48-49	0.5687625133731302
1103	50-51	0.5596482525415425
1103	52-53	0.5105475856300856
1103	54-55	0.4736384615631195
1103	56-57	0.46580190418916345
1103	58-59	0.4188066112717479
1103	60-61	0.4281770004038137
1103	62-63	0.4184810666744099
1103	64-65	0.4290679348535562
1103	66-67	0.40547219379528343
1103	68-69	0.4435731654919195
1103	70-71	0.41556101880492236
1103	72-73	0.4132933090737225
1103	74-75	0.4953669871398567
1103	76-77	0.426833426778348
1103	78-79	0.44116786157291443
1103	80-81	0.38475033398059466
1103	82-83	0.4230246937058375
1103	84-85	0.40349675671441076
1103	86-87	0.41496500967882
1103	88-89	0.3514389151940165
1103	90-91	0.36910189028575147
1103	92-93	0.3959107889167477
1103	94-95	0.3943939169101789
1103	96-97	0.45162605104027165
1103	98-99	0.38995534851331826
1103	100	0.4228986698805066
1104	1	0.36457944932385544
1104	2	0.43198804950694836
1104	3	0.43221172706164523
1104	4	0.39362095615037873
1104	5	0.4057724850783089
1104	6	0.4008706535304398
1104	7	0.36352651863574437
1104	8	0.4265854982284907
1104	9	0.49827979245125675
1104	10-11	0.5327831574725934
1104	12-13	0.576886006831586
1104	14-15	0.7305472637362769
1104	16-17	0.6810009101128713
1104	18-19	0.6920420202892856
1104	20-21	0.7333902129286329
1104	22-23	0.6885409394142528
1104	24-25	0.6873824293000794
1104	26-27	0.6834611449073691
1104	28-29	0.7320406586436761
1104	30-31	0.7392019421558231
1104	32-33	0.7698850783508604
1104	34-35	0.6060389656726102
1104	36-37	0.7135353679791123
1104	38-39	0.7638248950345243
1104	40-41	0.733633148772654
1104	42-43	0.6882240515271931
1104	44-45	0.7221217374547777
1104	46-47	0.6393309230418964
1104	48-49	0.5859578512416164
1104	50-51	0.6452834624863968
1104	52-53	0.5988432610994678
1104	54-55	0.5619760808135794
1104	56-57	0.5855892900415043
1104	58-59	0.6640737249977064
1104	60-61	0.6289940766925852
1104	62-63	0.6089452993880968
1104	64-65	0.5343093302917126
1104	66-67	0.47854266516672084
1104	68-69	0.5196217625842081
1104	70-71	0.5753580812627206
1104	72-73	0.5533006169512333
1104	74-75	0.6182646769573097
1104	76-77	0.6025622954347476
1104	78-79	0.575480489482942
1104	80-81	0.6092975423255034
1104	82-83	0.5844661238804498
1104	84-85	0.5165657866210864
1104	86-87	0.5690882284734577
1104	88-89	0.6566342010560149
1104	90-91	0.6255825855707293
1104	92-93	0.5894270894808251
1104	94-95	0.6645220470628672
1104	96-97	0.6195070718875542
1104	98-99	0.5983220342250846
1104	100	0.6161057532389584
1105	1	0.24383993309148622
1105	2	0.2578821592711016
1105	3	0.24879054387890065
1105	4	0.2732845671017614
1105	5	0.2379364455420685
1105	6	0.18055992678530686
1105	7	0.20024239019755186
1105	8	0.2534284716287729
1105	9	0.3175606080601341
1105	10-11	0.4126804045840089
1105	12-13	0.35815436366759457
1105	14-15	0.3965535876904056
1105	16-17	0.4232969680592831
1105	18-19	0.4151808053945629
1105	20-21	0.44177642398194195
1105	22-23	0.4929069839958444
1105	24-25	0.5224885822774681
1105	26-27	0.5119999703976035
1105	28-29	0.543868776758373
1105	30-31	0.5282989869770702
1105	32-33	0.46611751398134516
1105	34-35	0.49641119569222525
1105	36-37	0.4975380511125138
1105	38-39	0.5082224249779728
1105	40-41	0.4458853966815184
1105	42-43	0.4581434072533952
1105	44-45	0.4099169631785955
1105	46-47	0.433234327944767
1105	48-49	0.44207531085797314
1105	50-51	0.46352105921557296
1105	52-53	0.39618318963110255
1105	54-55	0.3968761521380486
1105	56-57	0.3968009760871354
1105	58-59	0.408761735107511
1105	60-61	0.47000841595610865
1105	62-63	0.4161696124370877
1105	64-65	0.4005828458611518
1105	66-67	0.4621908957884955
1105	68-69	0.37021436294348575
1105	70-71	0.43043536708754004
1105	72-73	0.42853167009752724
1105	74-75	0.46474145825209234
1105	76-77	0.4046272872755381
1105	78-79	0.4547169099724542
1105	80-81	0.4500580557862861
1105	82-83	0.4123633917834404
1105	84-85	0.3931281310693038
1105	86-87	0.43029760662552263
1105	88-89	0.45641782700509026
1105	90-91	0.3839383322873182
1105	92-93	0.4240165051594218
1105	94-95	0.3844749192955419
1105	96-97	0.41440098023682737
1105	98-99	0.3121994173624252
1105	100	0.3730727627112671
1106	1	0.21270706849729848
1106	2	0.3083709924417306
1106	3	0.34201769266135074
1106	4	0.28175597513421025
1106	5	0.21248207569571775
1106	6	0.30378022117918846
1106	7	0.27930536276210916
1106	8	0.27368392604351044
1106	9	0.3373777615035607
1106	10-11	0.41352141162988687
1106	12-13	0.3606768867359449
1106	14-15	0.3682548601427982
1106	16-17	0.3459513929743565
1106	18-19	0.37624742527274435
1106	20-21	0.378744268506054
1106	22-23	0.41681369155222825
1106	24-25	0.46881876479720574
1106	26-27	0.539959990362405
1106	28-29	0.5277179522633446
1106	30-31	0.4888014717492126
1106	32-33	0.5241540004084726
1106	34-35	0.43709222861478025
1106	36-37	0.40805834834334576
1106	38-39	0.4593904977488137
1106	40-41	0.4886606512948788
1106	42-43	0.5076238479838437
1106	44-45	0.4495433282483461
1106	46-47	0.41735064598135097
1106	48-49	0.4275738120992685
1106	50-51	0.4368621599735292
1106	52-53	0.5191017086208518
1106	54-55	0.44044611382232546
1106	56-57	0.4007828967451914
1106	58-59	0.3525978459139836
1106	60-61	0.3396980234075073
1106	62-63	0.3882304909136849
1106	64-65	0.395614202188284
1106	66-67	0.43721555942899926
1106	68-69	0.33415231813872026
1106	70-71	0.4251409383418263
1106	72-73	0.40508788996834255
1106	74-75	0.478675042036496
1106	76-77	0.4394875795914466
1106	78-79	0.4769285349177679
1106	80-81	0.4319536466272531
1106	82-83	0.40146597695816766
1106	84-85	0.44987899150446253
1106	86-87	0.4651146487879849
1106	88-89	0.4430876848630767
1106	90-91	0.35753445816522955
1106	92-93	0.4048396504855205
1106	94-95	0.3294649611165781
1106	96-97	0.31364595086623126
1106	98-99	0.2537337657827976
1106	100	0.2587918977294841
1107	1	0.1900007680633955
1107	2	0.09638450833640277
1107	3	0.10932691771178327
1107	4	0.0649505654847431
1107	5	0.15232468296751023
1107	6	0.1949378305953573
1107	7	0.06929883837496931
1107	8	0.11492906254188284
1107	9	0.0790650635086223
1107	10-11	0.18881811632975598
1107	12-13	0.26821559696288944
1107	14-15	0.19249788888332375
1107	16-17	0.1617333254615474
1107	18-19	0.306067875806427
1107	20-21	0.30356951878727756
1107	22-23	0.21517365745763328
1107	24-25	0.2207638409835866
1107	26-27	0.26708314514921483
1107	28-29	0.32029503906966283
1107	30-31	0.41403927001721286
1107	32-33	0.20304790761062463
1107	34-35	0.2613603516830594
1107	36-37	0.22605928904581418
1107	38-39	0.2381911230870415
1107	40-41	0.30348280686214935
1107	42-43	0.17702322135880877
1107	44-45	0.26086516681937155
1107	46-47	0.343498999007835
1107	48-49	0.3035498986063345
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2216	2	-0.6721184727832785
2216	3	-0.7678165185209807
2216	4	-0.7292436726130305
2216	5	-0.6263147517770946
2216	6	-0.6656906813562031
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2216	12-13	-1.0263156052494509
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2216	64-65	-1.3040402435558534
2216	66-67	-1.291753829280502
2216	68-69	-1.237989685160649
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2216	74-75	-1.2061473875282793
2216	76-77	-1.179503300840853
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2216	80-81	-1.1828409484040456
2216	82-83	-1.2154751216069002
2216	84-85	-1.249870876862758
2216	86-87	-1.3665670785210686
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2216	90-91	-1.3346004174620667
2216	92-93	-1.2512595259491945
2216	94-95	-1.1609016750383283
2216	96-97	-1.1114407839281455
2216	98-99	-1.1534233157694338
2216	100	-1.1970707038682136
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	154297.0
3	31621.0
4	18722.0
5	14846.0
6	13090.0
7	12525.0
8	13782.0
9	14942.0
10	16730.0
11	17978.0
12	20063.0
13	21417.0
14	22326.0
15	22683.0
16	22828.0
17	23555.0
18	23297.0
19	23104.0
20	23679.0
21	25477.0
22	29488.0
23	36976.0
24	45397.0
25	55085.0
26	65963.0
27	75766.0
28	92774.0
29	112195.0
30	133736.0
31	161129.0
32	201724.0
33	261299.0
34	354728.0
35	509519.0
36	799780.0
37	1188507.0
38	973127.0
39	196253.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80925219940594	17.987150895400426	12.059962259379525	24.143634645814107
2	18.61115181724179	19.992799876726203	35.4980834562239	25.8979648498081
3	19.114360328730147	25.058028212909523	28.63968825999797	27.187923198362355
4	13.919015743268115	17.325180390719993	35.37279890714415	33.38300495886774
5	14.150276066365757	35.289182392570595	32.0362258379722	18.524315703091457
6	31.273196323088314	35.32313723538191	18.27858673534629	15.125079706183483
7	28.174817710796813	30.54883939407414	21.861931133301386	19.41441176182766
8	26.86187798643073	32.26994827582656	20.47643504884374	20.391738688898975
9	25.78997727359742	17.235685173481755	20.70906307370775	36.265274479213076
10-11	24.89540729518664	25.600420843135325	27.28193914737373	22.222232714304305
12-13	25.54172910544576	23.9575288398701	26.70953922048076	23.791202834203375
14-15	23.513106073891034	25.63480191642914	24.275255491124664	26.576836518555158
16-17	23.31648752814943	28.006738436532668	24.379145905573125	24.297628129744776
18-19	23.221347673760995	27.450842703863326	25.989137888348907	23.338671734026775
20-21	24.528051598146465	27.14443205205149	25.142367988817654	23.1851483609844
22-23	24.0063378978733	27.273875403040766	25.014786568155365	23.705000130930564
24-25	23.74489662389797	27.139219172723283	25.418204648859216	23.697679554519528
26-27	23.805325213769265	26.746174118554645	25.465048352526104	23.983452315149982
28-29	23.782550423843514	26.774425379253596	25.459234278572296	23.983789918330594
30-31	23.973467577922786	27.61250267204309	25.014349622565867	23.399680127468258
32-33	23.489972519867624	27.0906810034304	25.461924158611392	23.95742231809058
34-35	23.63015747800992	26.334812401924463	25.67088410254086	24.36414601752476
36-37	24.256376950387644	26.92320272870762	25.32584764515469	23.494572675750053
38-39	24.105425525148767	27.039373164641184	25.206617209630604	23.648584100579445
40-41	23.77628194211346	26.647466948856906	25.58077272240336	23.99547838662627
42-43	23.42463301033062	26.990476493041438	25.59681516548612	23.98807533114182
44-45	23.712199999038322	27.065274658998877	25.334587065278093	23.887938276684707
46-47	23.704371853083206	27.12181186847004	25.253599420246054	23.920216858200703
48-49	23.555832919519577	26.797127892146055	25.788452570075325	23.858586618259043
50-51	23.604256197410525	26.573812253128672	25.569954690031597	24.251976859429206
52-53	23.433407280840477	26.678904734428976	25.538417319598743	24.3492706651318
54-55	23.511240233471145	27.37709512123881	25.42487115763149	23.686793487658555
56-57	23.079661545844242	26.934078013742997	25.458652767720746	24.527607672692014
58-59	23.17516725780963	26.324226607510028	25.84815940020304	24.652446734477305
60-61	23.644191195617154	26.98407412677703	25.405399049710404	23.966335627895415
62-63	23.447743498924297	27.003575600219463	25.384332388675368	24.16434851218087
64-65	23.461206268489114	26.423740803901367	25.646263100316414	24.4687898272931
66-67	23.122442973127885	26.716086349850904	25.62907196816846	24.53239870885275
68-69	23.852178257599952	26.66664091285253	25.379473866746334	24.10170696280119
70-71	23.727988590391607	26.197752638845362	25.71554491327206	24.358713857490976
72-73	23.19278034176602	26.586968880552565	26.04043273718961	24.179818040491803
74-75	23.26284496206476	26.256504124604508	25.931818293018644	24.54883262031209
76-77	23.510869809654945	26.511584112167984	25.533010269235913	24.444535808941158
78-79	23.46963638815143	26.888280038309354	25.77843657916607	23.863646994373152
80-81	23.358520122911123	26.309449569174237	26.017483301929232	24.314547005985403
82-83	23.30032865066291	26.347739369063856	26.02521694969167	24.326715030581568
84-85	23.3046409652252	26.545246304336484	25.699313181909307	24.450799548529012
86-87	23.69876115328576	26.562756175992767	25.673360340595718	24.065122330125757
88-89	23.379095672746203	26.307686327749234	25.904823635274848	24.408394364229714
90-91	23.115026239002535	26.232457687737387	26.02512414836576	24.627391924894322
92-93	23.60939836440982	26.45075078351728	25.61472737990586	24.325123472167036
94-95	23.565877043129994	26.04949618984591	25.91380006201928	24.470826705004818
96-97	23.286504362529868	26.177323766008637	25.97933031014657	24.556841561314922
98-99	23.411342117778172	26.282024667906946	25.944647497901798	24.361985716413088
100	23.575508167758453	26.17906534635664	25.830556476742426	24.41487000914248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12344.0
1	8747.5
2	7021.5
3	8221.5
4	6512.0
5	5103.0
6	4450.0
7	4268.0
8	4467.5
9	4439.0
10	3830.0
11	3153.5
12	2844.0
13	2729.0
14	2738.5
15	2727.0
16	2728.0
17	2879.5
18	3203.0
19	3676.0
20	4305.5
21	5163.5
22	6589.5
23	8775.5
24	11523.0
25	14843.5
26	19303.5
27	24969.5
28	32439.0
29	41034.5
30	48739.5
31	56199.5
32	65198.5
33	73475.5
34	81172.5
35	90931.0
36	102085.0
37	114052.5
38	125641.5
39	134886.0
40	144908.0
41	155263.0
42	162090.5
43	168354.0
44	174536.5
45	180623.0
46	188338.0
47	194279.0
48	198571.5
49	204784.5
50	212010.0
51	216111.5
52	214831.0
53	216534.0
54	219740.0
55	216752.5
56	209117.5
57	195020.0
58	179959.0
59	169025.0
60	156966.0
61	140978.5
62	121652.0
63	100545.5
64	81280.0
65	65075.0
66	50182.0
67	37526.0
68	27605.0
69	19917.0
70	14492.5
71	11147.5
72	8670.5
73	6674.5
74	5299.5
75	3721.5
76	2201.0
77	1420.0
78	1003.5
79	627.0
80	372.5
81	235.0
82	181.0
83	138.5
84	100.0
85	81.5
86	55.0
87	45.0
88	51.0
89	39.0
90	28.5
91	23.5
92	20.0
93	14.5
94	10.5
95	10.5
96	12.5
97	14.0
98	14.0
99	16.0
100	24.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03159208139848985
2	0.04968635168915587
3	0.0629097473233772
4	0.06242952024457279
5	0.05750719268682763
6	0.07700784227970654
7	0.10122500782512873
8	0.08676674255969608
9	0.10414067223215549
10-11	0.10422642706765628
12-13	0.10066760139437363
14-15	0.09382436552141084
16-17	0.10425215351830652
18-19	0.1047752580148613
20-21	0.12194337608211885
22-23	0.11795577623133224
24-25	0.12810057327107532
26-27	0.13320298598337213
28-29	0.11437122410739935
30-31	0.10942317009900396
32-33	0.09360140294910879
34-35	0.1080768191816416
36-37	0.11272473126578426
38-39	0.09003400179227607
40-41	0.09712592668819113
42-43	0.126102485603907
44-45	0.1273802326528687
46-47	0.12717442104766682
48-49	0.11489432860395415
50-51	0.11475712086715291
52-53	0.10974046299035688
54-55	0.11981665616169933
56-57	0.10552132508371814
58-59	0.08810451799350835
60-61	0.08184441500195093
62-63	0.09499920676777161
64-65	0.1266684675182122
66-67	0.11863323943178845
68-69	0.10625024118547485
70-71	0.1030858877554958
72-73	0.11203011709822787
74-75	0.09799205052674909
76-77	0.0911059372360359
78-79	0.08307070914961218
80-81	0.08407404072497138
82-83	0.08727269608915073
84-85	0.0735261959583746
86-87	0.09813783374710043
88-89	0.05837331652538558
90-91	0.02678123512689572
92-93	0.02968832405037239
94-95	0.0298855601720242
96-97	0.03167783623399064
98-99	0.030648778207981203
100	0.02987698468847412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5830575.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.435201853210046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95183355014255	43.212756276799844
2	13.183323320150938	14.616403786973969
3	3.7418694325377104	6.222938619032535
4	1.5314428351659124	3.395833707762985
5	0.8146041235882986	2.2578872010787303
6	0.5106388896608572	1.6984421953469202
7	0.3668928227919208	1.423714438297491
8	0.2531233973916589	1.122555730254153
9	0.20552329027201005	1.0253902573588283
>10	1.2601519103741012	13.234295976194312
>50	0.11703245225493965	4.48503468295526
>100	0.061084222128948415	5.9084995211968305
>500	0.002045801965821555	0.7546689362118826
>1k	4.0295503342947455E-4	0.3747611228597205
>5k	0.0	0.0
>10k+	3.0996541025114635E-5	0.26681754767654703
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15528	0.2663202171312435	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.715096710015736E-5	0.0
6	0.0	0.0	0.0	1.715096710015736E-5	0.0
7	0.0	0.0	0.0	1.715096710015736E-5	0.0
8	0.0	0.0	0.0	1.715096710015736E-5	0.0
9	1.715096710015736E-5	0.0	0.0	1.715096710015736E-5	0.0
10-11	1.715096710015736E-5	0.0	0.0	1.715096710015736E-5	0.0
12-13	1.715096710015736E-5	0.0	0.0	2.5726450650236038E-5	0.0
14-15	1.715096710015736E-5	8.57548355007868E-6	0.0	7.717935195070812E-5	0.0
16-17	1.715096710015736E-5	1.715096710015736E-5	0.0	1.286322532511802E-4	0.0
18-19	1.715096710015736E-5	1.715096710015736E-5	0.0	1.9723612165180966E-4	0.0
20-21	1.715096710015736E-5	1.715096710015736E-5	0.0	3.9447224330361926E-4	0.0
22-23	1.715096710015736E-5	1.715096710015736E-5	0.0	0.0012948980160618806	0.0
24-25	2.5726450650236038E-5	1.715096710015736E-5	0.0	0.0028041831208757283	0.0
26-27	3.430193420031472E-5	5.145290130047208E-5	0.0	0.005042384327446263	0.0
28-29	5.145290130047208E-5	5.145290130047208E-5	1.715096710015736E-5	0.010796533789549059	0.0
30-31	5.145290130047208E-5	5.145290130047208E-5	1.715096710015736E-5	0.02437152424932361	0.0
32-33	5.145290130047208E-5	5.145290130047208E-5	1.715096710015736E-5	0.05352816831959112	0.0
34-35	5.145290130047208E-5	6.860386840062943E-5	1.715096710015736E-5	0.10300013291999502	0.0
36-37	5.145290130047208E-5	8.575483550078681E-5	1.715096710015736E-5	0.17596034696406443	0.0
38-39	5.145290130047208E-5	1.0290580260094417E-4	1.715096710015736E-5	0.26812106867676	0.0
40-41	6.860386840062943E-5	1.0290580260094417E-4	1.715096710015736E-5	0.3730335344284226	0.0
42-43	6.860386840062943E-5	1.0290580260094417E-4	1.715096710015736E-5	0.4836229702902372	0.0
44-45	6.860386840062943E-5	1.0290580260094417E-4	1.715096710015736E-5	0.6192613936018316	0.0
46-47	6.860386840062943E-5	1.0290580260094417E-4	1.715096710015736E-5	0.778979774722047	0.0
48-49	6.860386840062943E-5	1.4578322035133757E-4	1.715096710015736E-5	0.9453613065606736	0.0
50-51	6.860386840062943E-5	2.2296257230204568E-4	1.715096710015736E-5	1.1133550293067151	0.0
52-53	6.860386840062943E-5	2.2296257230204568E-4	1.715096710015736E-5	1.2865454950841042	1.715096710015736E-5
54-55	8.575483550078681E-5	2.2296257230204568E-4	1.715096710015736E-5	1.4798626207535277	1.715096710015736E-5
56-57	8.575483550078681E-5	2.2296257230204568E-4	1.715096710015736E-5	1.7084078328466745	1.715096710015736E-5
58-59	8.575483550078681E-5	2.572645065023604E-4	1.715096710015736E-5	1.9491731089986837	1.715096710015736E-5
60-61	8.575483550078681E-5	2.572645065023604E-4	1.715096710015736E-5	2.195881195250897	1.715096710015736E-5
62-63	8.575483550078681E-5	2.572645065023604E-4	1.715096710015736E-5	2.449175252869571	1.715096710015736E-5
64-65	8.575483550078681E-5	2.572645065023604E-4	1.715096710015736E-5	2.734155722205786	1.715096710015736E-5
66-67	8.575483550078681E-5	3.0014192425275384E-4	1.715096710015736E-5	3.06375443245306	1.715096710015736E-5
68-69	8.575483550078681E-5	3.2586837490298983E-4	1.715096710015736E-5	3.4098523730506853	1.715096710015736E-5
70-71	8.575483550078681E-5	3.601703091033046E-4	1.715096710015736E-5	3.779987394039181	1.715096710015736E-5
72-73	8.575483550078681E-5	3.601703091033046E-4	1.715096710015736E-5	4.179510597153797	1.715096710015736E-5
74-75	8.575483550078681E-5	3.601703091033046E-4	1.715096710015736E-5	4.576769872611192	1.715096710015736E-5
76-77	8.575483550078681E-5	3.601703091033046E-4	1.715096710015736E-5	5.000184372896326	1.715096710015736E-5
78-79	8.575483550078681E-5	3.687457926533832E-4	1.715096710015736E-5	5.463869343932631	1.715096710015736E-5
80-81	8.575483550078681E-5	3.773212762034619E-4	1.715096710015736E-5	5.95743987514096	1.715096710015736E-5
82-83	8.575483550078681E-5	3.773212762034619E-4	1.715096710015736E-5	6.469919347577211	1.715096710015736E-5
84-85	8.575483550078681E-5	3.773212762034619E-4	1.715096710015736E-5	6.98533163538759	1.715096710015736E-5
86-87	8.575483550078681E-5	3.773212762034619E-4	1.715096710015736E-5	7.524429408763286	1.715096710015736E-5
88	8.575483550078681E-5	3.773212762034619E-4	1.715096710015736E-5	7.962027758840252	1.715096710015736E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	800	0.0	45.236404	2
TACCTGG	6265	0.0	42.535282	2
GTACCTG	7645	0.0	35.422085	1
TAACGCA	1140	0.0	34.633415	4
GTATAAC	1490	0.0	31.868689	1
ATAACGC	1255	0.0	31.086117	3
ACCTGGG	8680	0.0	30.59573	3
GGTATCA	6700	0.0	28.980398	1
GTATCAA	9450	0.0	27.760769	1
GCTACGC	1280	0.0	26.069681	2
ATCAACG	10025	0.0	25.037388	3
TCAACGC	10130	0.0	24.870026	4
CAACGCA	10490	0.0	23.88231	5
AACGCAG	11215	0.0	22.836843	6
GTACATG	20210	0.0	22.588219	1
TACGCCC	1665	0.0	22.019186	4
TATCAAC	11675	0.0	21.899256	2
GGCTACG	1975	0.0	21.186148	1
TACATGG	20680	0.0	21.113125	2
ACATGGG	20275	0.0	20.51703	3
>>END_MODULE