FastQCFastQC Report
Wed 25 May 2016
SRR2049537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830575
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258390.44316383890096606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219650.37672099235495643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190960.327514867744605No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123190.21128276370683852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117320.20121514601904614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79780.13683041552505543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8500.055.8459972
GGTATCA118950.046.8488921
TACCTGG60900.044.7610552
GTACCTG74400.037.703821
TAACGCA13600.036.6316574
GTATCAA191450.035.843811
GTATAAC15400.035.4526981
ATAACGC14050.034.7893753
ACCTGGG85550.031.1496143
ATCAACG227100.029.9461523
TCAACGC230900.029.5550924
CAACGCA236700.028.9095635
TATCAAC241150.028.1819232
AACGCAG247300.028.0505056
ACGCAGA275250.025.2192177
CGCAGAG284550.024.4449428
GCTACGC11900.024.091982
TATCACG10450.023.8368682
GTCACGC18550.023.5626938
GCAGAGT313900.021.9946179
Produced by FastQC (version 0.11.5)