FastQCFastQC Report
Wed 25 May 2016
SRR2049536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122590.1299743497068076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100050.041.9487342
TATAACG13450.039.83522
GTACCTG121550.035.233911
ATAACGC18200.032.7976073
ACCTGGG126100.032.6511763
TAACGCA17850.032.1253284
GTATCAA145450.029.6382851
GGTATCA101950.026.6986181
ATCAACG156350.025.9732153
GTATAAC28050.025.809841
TCAACGC158550.025.5841244
CAACGCA164700.024.9425875
AACGCAG176500.023.6979796
GTACATG325100.023.1077291
TATCAAC181950.022.9632932
TACATGG338150.021.5708262
TATCACG15800.021.4170632
ACATGGG338100.020.6716733
CCTGGGG142800.020.1112464
ACGCAGA207900.019.827227
Produced by FastQC (version 0.11.5)