##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9431861 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.069430518537114 31.0 30.0 33.0 26.0 34.0 2 30.256959151539657 31.0 30.0 34.0 26.0 34.0 3 30.33860412065021 31.0 31.0 34.0 26.0 34.0 4 34.01456859892231 37.0 35.0 37.0 30.0 37.0 5 33.90852335504096 37.0 35.0 37.0 30.0 37.0 6 34.010125997403904 37.0 35.0 37.0 30.0 37.0 7 33.92028699320314 37.0 35.0 37.0 30.0 37.0 8 33.91673700449996 37.0 35.0 37.0 30.0 37.0 9 35.37010257042592 39.0 35.0 39.0 29.0 39.0 10-11 35.29331173349565 39.0 35.0 39.0 29.0 39.0 12-13 35.206376663099675 39.0 35.0 39.0 29.0 39.0 14-15 36.24747624037292 40.0 36.0 41.0 28.5 41.0 16-17 36.216214965424115 40.0 36.0 41.0 28.0 41.0 18-19 36.196995799662446 40.0 36.0 41.0 28.0 41.0 20-21 36.195070516836495 40.0 36.0 41.0 28.0 41.0 22-23 36.121362051455165 40.0 36.0 41.0 27.5 41.0 24-25 36.09517777032549 39.5 36.0 41.0 27.5 41.0 26-27 36.05077651165554 39.0 36.0 41.0 27.5 41.0 28-29 35.972203258720626 39.0 36.0 41.0 27.0 41.0 30-31 35.89521156005162 39.0 36.0 41.0 27.0 41.0 32-33 35.850991230680776 39.0 35.5 41.0 27.0 41.0 34-35 35.78899959403558 39.0 35.0 41.0 27.0 41.0 36-37 35.682589363859364 39.0 35.0 41.0 27.0 41.0 38-39 35.58393465510147 39.0 35.0 41.0 27.0 41.0 40-41 35.50908097564203 39.0 35.0 41.0 27.0 41.0 42-43 35.30049806713649 39.0 35.0 41.0 26.0 41.0 44-45 35.15154692165205 39.0 35.0 40.0 26.0 41.0 46-47 34.99808712193702 39.0 34.0 40.0 26.0 41.0 48-49 34.83974154199261 38.0 34.0 40.0 25.0 41.0 50-51 34.66615490834735 38.0 34.0 40.0 24.0 41.0 52-53 34.50590673463063 38.0 34.0 40.0 24.0 41.0 54-55 34.29925403904913 38.0 33.0 40.0 24.0 41.0 56-57 34.08111267755112 38.0 33.0 40.0 23.0 41.0 58-59 33.858957633069444 37.5 33.0 40.0 23.0 41.0 60-61 33.60335388742476 37.0 33.0 40.0 22.5 41.0 62-63 33.313078087134656 36.5 33.0 40.0 22.0 41.0 64-65 32.99234424680347 36.0 32.0 39.0 21.0 41.0 66-67 32.71765132034919 35.5 32.0 39.0 20.0 41.0 68-69 32.40041922797632 35.0 32.0 39.0 20.0 40.0 70-71 32.075693227455325 35.0 31.5 38.0 18.5 40.0 72-73 31.726262028246598 35.0 31.0 37.5 18.0 40.0 74-75 31.38418563420305 35.0 31.0 37.0 15.5 39.0 76-77 31.081732703652015 35.0 31.0 36.5 13.0 39.0 78-79 30.75676321989902 35.0 31.0 36.0 9.5 39.0 80-81 30.425048460743856 34.5 30.5 35.5 7.0 37.5 82-83 30.122778685987846 34.0 30.0 35.0 4.5 37.0 84-85 29.816204087401204 34.0 30.0 35.0 2.0 36.5 86-87 29.5556691834199 34.0 30.0 35.0 2.0 36.0 88-89 29.253556429637797 34.0 30.0 35.0 2.0 36.0 90-91 28.992108927389832 34.0 29.0 35.0 2.0 36.0 92-93 28.75416537627092 34.0 29.0 35.0 2.0 35.0 94-95 28.49614169462421 34.0 29.0 35.0 2.0 35.0 96-97 28.215293408161976 34.0 29.0 35.0 2.0 35.0 98-99 27.90801258627539 34.0 29.0 35.0 2.0 35.0 100 27.669046861483647 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5253845783911579 1101 2 0.5940600208789775 1101 3 0.5555138144929401 1101 4 0.6021809288203315 1101 5 0.5465472800224944 1101 6 0.5861869604535812 1101 7 0.587290023988615 1101 8 0.6342701275846281 1101 9 0.7814080628821145 1101 10-11 0.7775251984867211 1101 12-13 0.784279627075783 1101 14-15 0.9765743733335199 1101 16-17 0.895803239917079 1101 18-19 0.8785709697325004 1101 20-21 0.8984323057264305 1101 22-23 0.9183428307706478 1101 24-25 0.9080591567345877 1101 26-27 0.9407803893204374 1101 28-29 0.9652544211938405 1101 30-31 1.0036642188467368 1101 32-33 1.0285747376904695 1101 34-35 0.979511374725277 1101 36-37 1.051561183693984 1101 38-39 1.0445331059357414 1101 40-41 1.0078230799262187 1101 42-43 0.9983828561866446 1101 44-45 0.9326092798191894 1101 46-47 0.8863208500596613 1101 48-49 0.9693164140747825 1101 50-51 0.934558432207325 1101 52-53 0.982311845728411 1101 54-55 0.9151641604356442 1101 56-57 0.9241019620459383 1101 58-59 0.920310869099616 1101 60-61 0.9100494996755657 1101 62-63 0.8960615238111487 1101 64-65 0.8497326701160475 1101 66-67 0.9165243936664211 1101 68-69 0.958976298296335 1101 70-71 0.9353106027496381 1101 72-73 0.9504622072118458 1101 74-75 0.9645222787867347 1101 76-77 0.9416855986257993 1101 78-79 0.9606537407206979 1101 80-81 0.9535828438586691 1101 82-83 0.9916293062333246 1101 84-85 0.9746415252664207 1101 86-87 1.0212832955669597 1101 88-89 1.0445619247558042 1101 90-91 0.9495234985250356 1101 92-93 0.9537238204573768 1101 94-95 0.9877824428769237 1101 96-97 0.9858893072165493 1101 98-99 0.9880208947209752 1101 100 1.0174049626926553 1102 1 0.40863502308721067 1102 2 0.46920842031739696 1102 3 0.4743253601666453 1102 4 0.5270531818800279 1102 5 0.5259936329107404 1102 6 0.5188261544273232 1102 7 0.43007056394044696 1102 8 0.5024536616991142 1102 9 0.4975211559617989 1102 10-11 0.5992845581075414 1102 12-13 0.6769045357025263 1102 14-15 0.8074281377711259 1102 16-17 0.800756594072439 1102 18-19 0.7536522696260235 1102 20-21 0.7376527483839297 1102 22-23 0.8504280601180483 1102 24-25 0.746468588435441 1102 26-27 0.7931886181186485 1102 28-29 0.8144717687864826 1102 30-31 0.8777722943528232 1102 32-33 0.8276486066184816 1102 34-35 0.8334990841005094 1102 36-37 0.7738935728662852 1102 38-39 0.8577524889936399 1102 40-41 0.7928594037365997 1102 42-43 0.8215621242255864 1102 44-45 0.7997671574897964 1102 46-47 0.738001829937815 1102 48-49 0.7412835701400908 1102 50-51 0.7834934025485154 1102 52-53 0.7142858185230239 1102 54-55 0.7319387656743785 1102 56-57 0.7616024028433088 1102 58-59 0.7003028834670175 1102 60-61 0.6790959007810784 1102 62-63 0.7072844425205247 1102 64-65 0.5932106322848867 1102 66-67 0.6278383519593334 1102 68-69 0.6637659076451996 1102 70-71 0.6485782873643586 1102 72-73 0.693395561600429 1102 74-75 0.6920694237334182 1102 76-77 0.6352735599334132 1102 78-79 0.668020304157654 1102 80-81 0.7085310208401836 1102 82-83 0.7122172336820825 1102 84-85 0.6458358423733053 1102 86-87 0.673867913558265 1102 88-89 0.6585544277584958 1102 90-91 0.6184289568678913 1102 92-93 0.5907437043172798 1102 94-95 0.5972283616412355 1102 96-97 0.5882004762982724 1102 98-99 0.5019903708907307 1102 100 0.5159387617702613 1103 1 0.33806777878565697 1103 2 0.40425690479916554 1103 3 0.4093852663590454 1103 4 0.3937816164326051 1103 5 0.3766713278735665 1103 6 0.40494449286756407 1103 7 0.4390567579477178 1103 8 0.4633813493533978 1103 9 0.6094786943927986 1103 10-11 0.6285564580479175 1103 12-13 0.5839524518182202 1103 14-15 0.7193373922801527 1103 16-17 0.7865555869223115 1103 18-19 0.725406855136896 1103 20-21 0.7464971772066207 1103 22-23 0.7261689994041731 1103 24-25 0.7783551490932226 1103 26-27 0.7711161079325919 1103 28-29 0.7658533538615728 1103 30-31 0.7331143983814954 1103 32-33 0.7554455286034383 1103 34-35 0.6698449661319188 1103 36-37 0.713188744542073 1103 38-39 0.7651436139165639 1103 40-41 0.6953665236094935 1103 42-43 0.7671715438735092 1103 44-45 0.7204033331747439 1103 46-47 0.734423693539469 1103 48-49 0.648248529283677 1103 50-51 0.6546557587658342 1103 52-53 0.638288733778694 1103 54-55 0.6323749934368905 1103 56-57 0.6487193931137796 1103 58-59 0.6285259848285776 1103 60-61 0.6515328111267706 1103 62-63 0.6840296765058227 1103 64-65 0.6269684770624764 1103 66-67 0.6374353763317657 1103 68-69 0.5846746950878483 1103 70-71 0.5925314501923964 1103 72-73 0.6135892683738469 1103 74-75 0.6061426844890327 1103 76-77 0.5658512333445564 1103 78-79 0.5794572981265809 1103 80-81 0.617051956903758 1103 82-83 0.7020372669546155 1103 84-85 0.6505173296160045 1103 86-87 0.6385010488926213 1103 88-89 0.7162401172388932 1103 90-91 0.5937668729359977 1103 92-93 0.6106463342341399 1103 94-95 0.6494574376452427 1103 96-97 0.7021659378611567 1103 98-99 0.6853589732880678 1103 100 0.7274793941659041 1104 1 0.21658259417362302 1104 2 0.3065595127376035 1104 3 0.2732469456898947 1104 4 0.28497786360316724 1104 5 0.24287768628084194 1104 6 0.2891573245646555 1104 7 0.30115897717401907 1104 8 0.3320883175823468 1104 9 0.4216960584419098 1104 10-11 0.4384704010630358 1104 12-13 0.4218286593306928 1104 14-15 0.5463886618895231 1104 16-17 0.4858913665366913 1104 18-19 0.4872202811806119 1104 20-21 0.510207187986687 1104 22-23 0.5003352354799091 1104 24-25 0.5290957217747874 1104 26-27 0.5696415591350501 1104 28-29 0.5318571072155933 1104 30-31 0.6086242748696549 1104 32-33 0.5547674200210864 1104 34-35 0.46088892837281037 1104 36-37 0.5343121147444876 1104 38-39 0.5509177464616286 1104 40-41 0.5022134701733805 1104 42-43 0.47192419233557814 1104 44-45 0.4617498325360714 1104 46-47 0.4738797063811333 1104 48-49 0.46661158431976446 1104 50-51 0.4381669676815534 1104 52-53 0.33124007394377486 1104 54-55 0.346328972228207 1104 56-57 0.4185992516876098 1104 58-59 0.4146752560588922 1104 60-61 0.371901090957536 1104 62-63 0.42874460782547885 1104 64-65 0.38720385511256694 1104 66-67 0.34203146455526223 1104 68-69 0.38000840524288293 1104 70-71 0.3872297917397489 1104 72-73 0.4045087876507729 1104 74-75 0.4426195020854671 1104 76-77 0.349195521610536 1104 78-79 0.42575951000879186 1104 80-81 0.4437118521455723 1104 82-83 0.44499997734309105 1104 84-85 0.38783714370563516 1104 86-87 0.4313853086740309 1104 88-89 0.45057274938659475 1104 90-91 0.43551905452384077 1104 92-93 0.4376647915033445 1104 94-95 0.45138675603510237 1104 96-97 0.4687518938648161 1104 98-99 0.4187188939719064 1104 100 0.410712944062432 1105 1 0.2835160748743455 1105 2 0.3158805796918571 1105 3 0.3550229731809651 1105 4 0.34771474955869053 1105 5 0.2746910666536948 1105 6 0.27419853609354305 1105 7 0.32286806289954484 1105 8 0.2917681458179757 1105 9 0.38334703551065274 1105 10-11 0.4879349065353509 1105 12-13 0.4195443234885943 1105 14-15 0.4813631225292454 1105 16-17 0.4603387159796597 1105 18-19 0.482590298076218 1105 20-21 0.490513104433397 1105 22-23 0.5465040750744166 1105 24-25 0.5196697895746851 1105 26-27 0.5902283298591229 1105 28-29 0.5555148097321236 1105 30-31 0.5106796072905055 1105 32-33 0.5140255561518785 1105 34-35 0.5252186573577973 1105 36-37 0.5323696862061098 1105 38-39 0.5793643975813438 1105 40-41 0.49894451937307593 1105 42-43 0.5441394507288848 1105 44-45 0.5059822922411072 1105 46-47 0.4601582740001504 1105 48-49 0.49552497893726866 1105 50-51 0.48375081434204503 1105 52-53 0.4506602245188418 1105 54-55 0.5111196828842992 1105 56-57 0.5402078252654263 1105 58-59 0.5712746061451455 1105 60-61 0.5407104447526905 1105 62-63 0.5224908734256886 1105 64-65 0.4864859873478693 1105 66-67 0.5184135491019219 1105 68-69 0.5503751757171926 1105 70-71 0.534126809469015 1105 72-73 0.4828132688695952 1105 74-75 0.5387032392458586 1105 76-77 0.4907293980548424 1105 78-79 0.5508326877460696 1105 80-81 0.5157785239428883 1105 82-83 0.586805395734455 1105 84-85 0.5862155397694409 1105 86-87 0.5177285204350177 1105 88-89 0.5704731317180602 1105 90-91 0.4801005692436071 1105 92-93 0.540178516580653 1105 94-95 0.5229345867606554 1105 96-97 0.5496862301587306 1105 98-99 0.4024900516787362 1105 100 0.4318194340926418 1106 1 0.22608241728756084 1106 2 0.3620103077633168 1106 3 0.377470876631012 1106 4 0.3391133515142144 1106 5 0.25459092893686375 1106 6 0.30979491968348327 1106 7 0.2797312041795337 1106 8 0.3286632786093193 1106 9 0.3110117397617387 1106 10-11 0.4302651715658925 1106 12-13 0.4208267891061723 1106 14-15 0.45187045704865625 1106 16-17 0.42336589033658356 1106 18-19 0.4367619299349812 1106 20-21 0.44028588124943724 1106 22-23 0.5395376135657557 1106 24-25 0.5493979445294244 1106 26-27 0.5905871022673992 1106 28-29 0.5635210541505771 1106 30-31 0.5328301105471098 1106 32-33 0.5490558332429458 1106 34-35 0.5103536226764334 1106 36-37 0.5362397521196485 1106 38-39 0.5222340291539922 1106 40-41 0.5230894207606624 1106 42-43 0.525668958371206 1106 44-45 0.4562213719095496 1106 46-47 0.48345735535505696 1106 48-49 0.5235423126936851 1106 50-51 0.47621712239175906 1106 52-53 0.5273322842663148 1106 54-55 0.45181498420678423 1106 56-57 0.45722939435432863 1106 58-59 0.4944509993910131 1106 60-61 0.4611833382077961 1106 62-63 0.39145820826010436 1106 64-65 0.4319256091331667 1106 66-67 0.4365394228253763 1106 68-69 0.3956309199737049 1106 70-71 0.4226334765788664 1106 72-73 0.43381007369055524 1106 74-75 0.443967171536503 1106 76-77 0.4129070458875965 1106 78-79 0.49242336880123005 1106 80-81 0.44311539755921814 1106 82-83 0.4361700375644766 1106 84-85 0.4128031086641677 1106 86-87 0.4065463591248175 1106 88-89 0.4435325840311819 1106 90-91 0.44753083998328336 1106 92-93 0.4569904048853033 1106 94-95 0.40172521338833533 1106 96-97 0.4084296407170278 1106 98-99 0.31194878535224646 1106 100 0.32933165424053 1107 1 0.16199381715623318 1107 2 0.10004634843885896 1107 3 0.08104860725674357 1107 4 0.10201357924923116 1107 5 0.16738664375979795 1107 6 0.11804768062074089 1107 7 0.029104068441696995 1107 8 0.08199097668181565 1107 9 0.10600809104688125 1107 10-11 0.13720412291352346 1107 12-13 0.23688465150956972 1107 14-15 0.08490603300805333 1107 16-17 0.13569647312498034 1107 18-19 0.1651441055776246 1107 20-21 0.23140306327183424 1107 22-23 0.16836329928475635 1107 24-25 0.13702096396318098 1107 26-27 0.25455678790732605 1107 28-29 0.2041516650128088 1107 30-31 0.310315043989732 1107 32-33 0.10594069528844585 1107 34-35 0.13287019659777854 1107 36-37 0.11774099028779261 1107 38-39 0.15518127790485892 1107 40-41 0.15522871037263286 1107 42-43 0.09041875182568049 1107 44-45 0.13693456863643405 1107 46-47 0.23226520174051046 1107 48-49 0.2375540558475464 1107 50-51 0.26990560313142 1107 52-53 0.3082825399696887 1107 54-55 0.3033284178082951 1107 56-57 0.17964670326051646 1107 58-59 0.28994421319927 1107 60-61 0.17780270046817037 1107 62-63 0.16539620781362885 1107 64-65 0.08971863611670727 1107 66-67 0.2754553672482132 1107 68-69 0.3280542866647451 1107 70-71 0.37119361901466874 1107 72-73 0.2993758538383453 1107 74-75 0.3935619101300567 1107 76-77 0.31825762087286336 1107 78-79 0.3905564907479935 1107 80-81 0.434087693826946 1107 82-83 0.3461287085679352 1107 84-85 0.34771798574675117 1107 86-87 0.37019470249414965 1107 88-89 0.41179964679351855 1107 90-91 0.43782656289304 1107 92-93 0.49919207023266665 1107 94-95 0.47057539846811736 1107 96-97 0.503117992991708 1107 98-99 0.48042604175655157 1107 100 0.5211851320742653 1108 1 0.18822852084363006 1108 2 0.3031483055923694 1108 3 0.1947653313403812 1108 4 0.1921907024942726 1108 5 0.15950164187020022 1108 6 0.09901486174996421 1108 7 0.18648434993258434 1108 8 0.2144267471074528 1108 9 0.12180379215853776 1108 10-11 0.24914955711432896 1108 12-13 0.11898470113560933 1108 14-15 0.24862856586693027 1108 16-17 0.19310572756054967 1108 18-19 0.1473824284508254 1108 20-21 0.1547175868941224 1108 22-23 0.22643445024540654 1108 24-25 0.22689522684182606 1108 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-1.2087507565464186 2215 74-75 -1.2984981570344374 2215 76-77 -1.3151328306797048 2215 78-79 -1.2509986481693396 2215 80-81 -1.1054957943229873 2215 82-83 -1.2021979414993673 2215 84-85 -1.1635114448830812 2215 86-87 -1.1371724164748045 2215 88-89 -1.2734273688485303 2215 90-91 -1.2857359163312942 2215 92-93 -1.342808869017837 2215 94-95 -1.396419772188647 2215 96-97 -1.4574949933258985 2215 98-99 -1.588627372493523 2215 100 -1.51282341196017 2216 1 -0.8485922920057867 2216 2 -0.8934206105796285 2216 3 -0.9199915875908715 2216 4 -0.9422259942728317 2216 5 -0.8402285239146678 2216 6 -0.886911885553971 2216 7 -0.870789829325247 2216 8 -0.941604095250625 2216 9 -1.0952378323655765 2216 10-11 -1.1192111699801544 2216 12-13 -1.2668031050807187 2216 14-15 -1.4459032954974873 2216 16-17 -1.4575601662581903 2216 18-19 -1.3792271728044554 2216 20-21 -1.3410325485286592 2216 22-23 -1.4287403433569068 2216 24-25 -1.480067776619535 2216 26-27 -1.5551995398737333 2216 28-29 -1.6052396844587733 2216 30-31 -1.5492945587371594 2216 32-33 -1.6083487264708864 2216 34-35 -1.5706823295349253 2216 36-37 -1.6285380788691342 2216 38-39 -1.5716907021101179 2216 40-41 -1.4685281652604516 2216 42-43 -1.5260767900469787 2216 44-45 -1.5949578730730707 2216 46-47 -1.6111169562992913 2216 48-49 -1.4933037414206254 2216 50-51 -1.5682093065119602 2216 52-53 -1.6401441795518181 2216 54-55 -1.5751603189022134 2216 56-57 -1.7253512737766243 2216 58-59 -1.7193570909928937 2216 60-61 -1.7700250904781285 2216 62-63 -1.6076848324046011 2216 64-65 -1.6889500762989726 2216 66-67 -1.6209253515109907 2216 68-69 -1.5908384620937532 2216 70-71 -1.8118246650542318 2216 72-73 -1.667623683896128 2216 74-75 -1.5144336529084903 2216 76-77 -1.4295331661684934 2216 78-79 -1.373096793435355 2216 80-81 -1.471940023297151 2216 82-83 -1.4676639643912388 2216 84-85 -1.5288572964671836 2216 86-87 -1.5996614665591338 2216 88-89 -1.5935781174985237 2216 90-91 -1.572724523589887 2216 92-93 -1.5220664908780606 2216 94-95 -1.4872955078290886 2216 96-97 -1.4930439766929915 2216 98-99 -1.4643991767956308 2216 100 -1.5702585472122763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 223038.0 3 48087.0 4 28768.0 5 22130.0 6 20146.0 7 20343.0 8 21978.0 9 24699.0 10 27063.0 11 29528.0 12 32044.0 13 33804.0 14 35629.0 15 36396.0 16 37586.0 17 38471.0 18 38034.0 19 38249.0 20 39689.0 21 42407.0 22 48500.0 23 58510.0 24 72274.0 25 89529.0 26 107334.0 27 124632.0 28 149981.0 29 182896.0 30 217369.0 31 263674.0 32 330842.0 33 424645.0 34 574394.0 35 818068.0 36 1263843.0 37 1850805.0 38 1663751.0 39 352462.0 40 263.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.857236167490846 19.04562376058701 13.18995779460394 23.907182277318203 2 16.727839937543227 20.281394924538468 37.98065622043168 25.010108917486622 3 18.49641664231083 25.821806525737003 29.304614842755015 26.377161989197152 4 13.401216114702677 17.13564960993498 36.78291333063661 32.68022094472573 5 14.021577712853246 36.49010345221553 33.63067407359395 15.857644761337275 6 31.035569014362164 36.120220185246154 18.469145747154926 14.375065053236757 7 27.87576499115218 31.188800442507837 22.51198122652865 18.423453339811335 8 26.288632554688668 33.857545568317086 20.64646345886484 19.207358418129406 9 26.037632161603575 16.789540970919617 20.67610194721793 36.496724920258885 10-11 24.753832131661973 26.06814249229923 28.10493617783604 21.07308919820276 12-13 25.148528080290518 23.97409433621764 27.768650173516455 23.108727409975387 14-15 22.908057629247118 25.656681571636124 24.98008431917766 26.455176479939098 16-17 22.867827765386167 28.438417580462914 25.33705299256763 23.356701661583283 18-19 22.935463129327232 27.872536574781293 26.77378475816099 22.418215537730486 20-21 23.86211810056849 27.301071882387497 26.186905862421046 22.64990415462297 22-23 23.614625997911634 27.155917680665624 26.092607260988803 23.13684906043394 24-25 23.331017015382923 27.207383597857483 26.190390507639865 23.271208879119733 26-27 23.27114705675027 27.155084007314066 26.19045748592093 23.383311450014734 28-29 23.236645677606983 27.163000878303258 26.295550372416503 23.30480307167326 30-31 23.389593256005103 27.444451124934005 26.0641529004507 23.10180271861019 32-33 23.226853286899 27.26884432482371 26.206588919791578 23.29771346848571 34-35 23.35148779425555 26.889126783183453 26.335291105616065 23.42409431694493 36-37 23.55535384555621 27.092042204420935 26.1669882834959 23.185615666526953 38-39 23.45627959530516 27.22507563834176 26.106672345909164 23.211972420443917 40-41 23.327201125435515 26.922684887235455 26.350018276706848 23.40009571062219 42-43 23.17651346766847 27.118418448399375 26.296764960320928 23.408303123611223 44-45 23.2667005727409 27.229135954546752 26.175961697388605 23.328201775323745 46-47 23.280833602389656 27.080926179349095 26.229910276992573 23.408329941268672 48-49 23.28572698458187 26.95115314246746 26.3179337767323 23.44518609621837 50-51 23.191178586362074 26.980908671029336 26.266258176685447 23.561654565923142 52-53 23.110734869353518 26.995359079619075 26.255970438039792 23.637935612987615 54-55 23.128537115484313 27.259688442991965 26.266170049885297 23.345604391638428 56-57 23.031357267163344 26.994546712200503 26.267309008232864 23.70678701240329 58-59 22.987259303401625 26.693828016459587 26.457867968272247 23.861044711866537 60-61 23.223386104982456 26.991629774047777 26.22892383089609 23.55606029007368 62-63 23.03419029719006 27.091865219448664 26.194168686763312 23.67977579659796 64-65 23.044930896183267 26.79507002523988 26.322171887772573 23.83782719080428 66-67 22.916739751134056 26.882571407084882 26.328378414309505 23.872310427471557 68-69 23.19359846050262 26.84403037221501 26.202620889855293 23.759750277427074 70-71 23.088456002828543 26.58419793302652 26.405827400428578 23.92151866371636 72-73 22.847766222173636 26.76927845019872 26.547951806461644 23.835003521166 74-75 22.929258139535747 26.68441838279471 26.49040103621659 23.895922441452953 76-77 22.984314702786143 26.63360591203417 26.38552026173413 23.996559123445557 78-79 22.90116441931828 26.822479638047458 26.522578529864905 23.753777412769352 80-81 22.822260396743026 26.64835860083042 26.618955766454484 23.91042523597207 82-83 22.824514367096025 26.55850082605437 26.645226056214995 23.971758750634613 84-85 22.801209569675265 26.64025593571709 26.466681212567977 24.09185328203967 86-87 22.935190400401567 26.67021822165258 26.490993810159658 23.903597567786193 88-89 22.816553700616733 26.45767470063307 26.617099086151434 24.10867251259875 90-91 22.75813008895266 26.455970900810673 26.608028046767444 24.17787096346922 92-93 22.918360386542492 26.530339759234955 26.510338575707877 24.04096127851468 94-95 22.787925334527888 26.224935495941942 26.69840448200268 24.28873468752749 96-97 22.75670132284545 26.260706659405606 26.71505663997149 24.267535377777445 98-99 22.749405209720177 26.378034215208775 26.663376593186356 24.209183981884692 100 22.841624573277493 26.147137207657302 26.682344531960045 24.32889368710516 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8316.0 1 6099.5 2 5219.5 3 6320.5 4 5468.0 5 4526.0 6 4151.5 7 4127.5 8 4238.0 9 4215.5 10 3707.0 11 3136.5 12 2901.0 13 2763.0 14 2760.0 15 2927.5 16 3105.0 17 3402.5 18 4020.5 19 4816.5 20 5946.5 21 7712.5 22 10109.5 23 13986.5 24 19426.5 25 25811.0 26 34340.0 27 45966.5 28 60590.5 29 77506.5 30 93993.5 31 110401.0 32 127890.0 33 143793.0 34 160598.5 35 180092.5 36 200874.0 37 223618.5 38 244726.0 39 259885.0 40 275567.0 41 291451.5 42 301132.0 43 310018.5 44 318740.5 45 324715.0 46 332573.5 47 335743.0 48 335708.0 49 338736.5 50 341619.0 51 342348.0 52 333920.5 53 329040.5 54 325552.5 55 310939.0 56 292181.5 57 267482.5 58 242581.0 59 224926.0 60 206555.5 61 183327.0 62 157448.0 63 130164.5 64 104974.0 65 84123.0 66 65796.0 67 49692.0 68 37367.5 69 28443.0 70 21201.5 71 15673.0 72 11964.0 73 9162.0 74 7181.0 75 5188.5 76 3199.0 77 2035.5 78 1378.0 79 852.0 80 502.5 81 302.0 82 193.5 83 151.5 84 112.0 85 85.0 86 69.0 87 59.5 88 45.0 89 36.5 90 29.0 91 22.5 92 22.5 93 20.0 94 16.5 95 17.5 96 17.5 97 14.0 98 18.5 99 26.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026484699042956632 2 0.048145323600506836 3 0.05986093306506532 4 0.05950045277384813 5 0.05502625621815249 6 0.07302906605599892 7 0.09757353294328659 8 0.0835360063088292 9 0.10066942250315182 10-11 0.09996966664373022 12-13 0.09520390514660892 14-15 0.08886899414654223 16-17 0.10064291659938585 18-19 0.10058990479185391 20-21 0.11618067738699711 22-23 0.11263418746311042 24-25 0.12445051936197957 26-27 0.12940182218546264 28-29 0.10905589045470453 30-31 0.10316627863790613 32-33 0.08878417525449114 34-35 0.10435374312662156 36-37 0.10808047319611687 38-39 0.08555045499504287 40-41 0.09186416127209678 42-43 0.12157197821299529 44-45 0.12425967685486458 46-47 0.12288667103978737 48-49 0.10945347901119408 50-51 0.10954890026475157 52-53 0.10626746937852456 54-55 0.1171454922840784 56-57 0.10139568426633938 58-59 0.08278323864187566 60-61 0.07765699685353718 62-63 0.09151958452313917 64-65 0.12352281273017064 66-67 0.11328093151500006 68-69 0.10141158780859896 70-71 0.09903135765041492 72-73 0.10884384322457678 74-75 0.09260632657754392 76-77 0.08587382702098768 78-79 0.0786801247389036 80-81 0.08057264626779381 82-83 0.0831702248368588 84-85 0.06918571000993334 86-87 0.09286078325369723 88-89 0.05444312633530116 90-91 0.023139653987691296 92-93 0.025647112483952 94-95 0.025610004218679643 96-97 0.027205659625391003 98-99 0.026368073066386367 100 0.025774340822028653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9431861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.85323787980736 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88581913734689 38.83072193553078 2 14.164789947907003 14.689804453726518 3 4.436074940640466 6.900745474490471 4 1.9838631643610987 4.114789143304137 5 1.0871662930371897 2.8186546203882874 6 0.689024467180923 2.143688976104395 7 0.4575602788050139 1.6608187386859152 8 0.3384771703528746 1.4040909784953348 9 0.24335335436071562 1.135679343526372 >10 1.5100844936464686 14.688414465952269 >50 0.13574278078712954 4.832753222155738 >100 0.06611670685506844 5.900183203456285 >500 0.001640443727647777 0.5659500074141591 >1k 2.663337961390328E-4 0.18347471824466613 >5k 0.0 0.0 >10k+ 2.0487195483024552E-5 0.1302307185246233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12259 0.1299743497068076 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0602361506387763E-5 0.0 0.0 0.0 3 0.0 1.0602361506387763E-5 0.0 1.0602361506387763E-5 0.0 4 1.0602361506387763E-5 2.1204723012775527E-5 0.0 2.1204723012775527E-5 0.0 5 1.0602361506387763E-5 2.1204723012775527E-5 0.0 2.1204723012775527E-5 0.0 6 1.0602361506387763E-5 2.1204723012775527E-5 0.0 2.1204723012775527E-5 0.0 7 1.0602361506387763E-5 2.1204723012775527E-5 0.0 2.1204723012775527E-5 0.0 8 1.0602361506387763E-5 2.1204723012775527E-5 0.0 2.1204723012775527E-5 0.0 9 1.0602361506387763E-5 2.1204723012775527E-5 0.0 4.2409446025551054E-5 1.0602361506387763E-5 10-11 1.0602361506387763E-5 3.180708451916329E-5 0.0 6.361416903832658E-5 1.0602361506387763E-5 12-13 1.0602361506387763E-5 4.7710626778744936E-5 0.0 8.481889205110211E-5 1.0602361506387763E-5 14-15 1.0602361506387763E-5 9.0120072804296E-5 0.0 8.481889205110211E-5 1.0602361506387763E-5 16-17 1.0602361506387763E-5 1.0602361506387763E-4 0.0 1.0602361506387763E-4 2.1204723012775527E-5 18-19 1.0602361506387763E-5 1.0602361506387763E-4 0.0 1.166259765702654E-4 2.1204723012775527E-5 20-21 1.0602361506387763E-5 1.1132479581707152E-4 0.0 2.014448686213675E-4 2.1204723012775527E-5 22-23 1.0602361506387763E-5 1.2192715732345927E-4 0.0 5.036121715534188E-4 2.1204723012775527E-5 24-25 1.0602361506387763E-5 1.2722833807665316E-4 0.0 0.0014843306108942868 2.1204723012775527E-5 26-27 1.0602361506387763E-5 1.2722833807665316E-4 1.5903542259581645E-5 0.0031542025481503594 2.1204723012775527E-5 28-29 1.5903542259581645E-5 1.3783069958304094E-4 2.1204723012775527E-5 0.007172497559071322 2.1204723012775527E-5 30-31 2.1204723012775527E-5 1.6433660334901035E-4 2.1204723012775527E-5 0.018331483044544444 2.1204723012775527E-5 32-33 2.1204723012775527E-5 2.1204723012775527E-4 2.1204723012775527E-5 0.0438460660096666 2.1204723012775527E-5 34-35 2.1204723012775527E-5 2.1204723012775527E-4 2.1204723012775527E-5 0.08528009477662998 2.1204723012775527E-5 36-37 2.1204723012775527E-5 2.332519531405308E-4 2.1204723012775527E-5 0.14775450995301986 2.1204723012775527E-5 38-39 2.1204723012775527E-5 2.597578569065002E-4 2.1204723012775527E-5 0.2288413707538735 2.650590376596941E-5 40-41 2.1204723012775527E-5 2.756613991660819E-4 2.1204723012775527E-5 0.3189349376544035 3.180708451916329E-5 42-43 2.1204723012775527E-5 3.445767489576023E-4 2.1204723012775527E-5 0.4146212502495531 3.180708451916329E-5 44-45 2.1204723012775527E-5 3.498779297107962E-4 2.1204723012775527E-5 0.53068530165998 3.180708451916329E-5 46-47 2.1204723012775527E-5 3.60480291217184E-4 2.1204723012775527E-5 0.6663902277609901 3.180708451916329E-5 48-49 2.1204723012775527E-5 3.60480291217184E-4 2.1204723012775527E-5 0.8099515037382337 3.710826527235717E-5 50-51 2.1204723012775527E-5 3.9228737573634726E-4 2.1204723012775527E-5 0.9563595137799422 4.2409446025551054E-5 52-53 2.1204723012775527E-5 4.1349209874912276E-4 2.1204723012775527E-5 1.106017147623359 4.2409446025551054E-5 54-55 2.1204723012775527E-5 4.1349209874912276E-4 2.1204723012775527E-5 1.273603374774077 4.2409446025551054E-5 56-57 2.650590376596941E-5 4.187932795023167E-4 2.1204723012775527E-5 1.470685371635566 4.2409446025551054E-5 58-59 3.180708451916329E-5 4.2409446025551054E-4 2.1204723012775527E-5 1.68631619995248 4.2409446025551054E-5 60-61 3.180708451916329E-5 4.2409446025551054E-4 2.1204723012775527E-5 1.9135407105766296 4.2409446025551054E-5 62-63 3.180708451916329E-5 4.2409446025551054E-4 2.1204723012775527E-5 2.1457748370125476 4.2409446025551054E-5 64-65 3.180708451916329E-5 4.2409446025551054E-4 2.1204723012775527E-5 2.3944638285063786 4.2409446025551054E-5 66-67 3.180708451916329E-5 4.2409446025551054E-4 2.1204723012775527E-5 2.675744479270846 4.2409446025551054E-5 68-69 3.180708451916329E-5 4.293956410087044E-4 2.1204723012775527E-5 2.9853811458841477 5.301180753193882E-5 70-71 3.180708451916329E-5 4.3999800251509223E-4 2.1204723012775527E-5 3.320214324617379 5.301180753193882E-5 72-73 3.180708451916329E-5 4.452991832682861E-4 2.1204723012775527E-5 3.674396813099769 5.301180753193882E-5 74-75 3.180708451916329E-5 4.559015447746738E-4 2.1204723012775527E-5 4.030249173519414 5.301180753193882E-5 76-77 4.2409446025551054E-5 4.7710626778744937E-4 2.1204723012775527E-5 4.410105280389523 5.301180753193882E-5 78-79 4.2409446025551054E-5 4.8770862929383715E-4 2.1204723012775527E-5 4.824095690129445 5.301180753193882E-5 80-81 4.2409446025551054E-5 5.195157138130005E-4 2.1204723012775527E-5 5.271504743337502 5.301180753193882E-5 82-83 4.7710626778744936E-5 5.195157138130005E-4 2.1204723012775527E-5 5.741523332457932 5.301180753193882E-5 84-85 5.301180753193882E-5 5.354192560725821E-4 2.1204723012775527E-5 6.205827248726418 5.301180753193882E-5 86-87 5.301180753193882E-5 5.407204368257759E-4 2.1204723012775527E-5 6.690694445136543 5.301180753193882E-5 88 5.301180753193882E-5 5.407204368257759E-4 2.1204723012775527E-5 7.081030986355715 5.301180753193882E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 10005 0.0 41.948734 2 TATAACG 1345 0.0 39.8352 2 GTACCTG 12155 0.0 35.23391 1 ATAACGC 1820 0.0 32.797607 3 ACCTGGG 12610 0.0 32.651176 3 TAACGCA 1785 0.0 32.125328 4 GTATCAA 14545 0.0 29.638285 1 GGTATCA 10195 0.0 26.698618 1 ATCAACG 15635 0.0 25.973215 3 GTATAAC 2805 0.0 25.80984 1 TCAACGC 15855 0.0 25.584124 4 CAACGCA 16470 0.0 24.942587 5 AACGCAG 17650 0.0 23.697979 6 GTACATG 32510 0.0 23.107729 1 TATCAAC 18195 0.0 22.963293 2 TACATGG 33815 0.0 21.570826 2 TATCACG 1580 0.0 21.417063 2 ACATGGG 33810 0.0 20.671673 3 CCTGGGG 14280 0.0 20.111246 4 ACGCAGA 20790 0.0 19.82722 7 >>END_MODULE