FastQCFastQC Report
Wed 25 May 2016
SRR2049536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263550.2794252375008495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219490.23271123270370503No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212280.22506693005759945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136070.14426633301741829No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95750.10151761142366283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14550.048.1289832
GGTATCA165750.043.781921
TACCTGG99050.041.1859052
TAACGCA18350.039.698954
GTATCAA274450.038.118891
GTACCTG123050.033.8091771
ATAACGC20850.033.586413
TATCACG19700.032.445622
ACCTGGG123050.032.42723
ATCAACG322850.031.8223863
TCAACGC327700.031.394444
CAACGCA338400.030.429225
TATCAAC344600.029.8956992
AACGCAG353050.029.4464076
GTATAAC28850.028.0573481
ACGCAGA405650.025.570217
CGCAGAG421800.024.5245768
GCAGAGT464600.022.052889
TCACGCA29450.021.863454
GTACATG325900.021.5884171
Produced by FastQC (version 0.11.5)