FastQCFastQC Report
Wed 25 May 2016
SRR2049535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764247
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.042.282811
GTATCAA132600.036.8179471
ATCAACG140150.033.8783873
TCAACGC139950.033.853474
CAACGCA152100.031.081925
TATCAAC164600.029.1879542
AACGCAG167350.028.5550866
ACGCAGA194750.024.0376437
GTACATG230550.023.8615191
CGCAGAG198700.023.4287768
TACATGG230750.022.6608962
ACATGGG234500.021.4039553
GCAGAGT229050.019.3565469
GTGGTAT51050.019.347121
CATGGGG165850.018.5097644
AGAGTAC195600.017.32387710-11
CAGAGTA217800.016.25393710-11
TGGTATC54750.016.0735662
GTACCGT5252.7284841E-1115.5927596
GAGTACA177600.015.4734121
Produced by FastQC (version 0.11.5)