##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049535_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8764247 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89419159455456 31.0 31.0 34.0 28.0 34.0 2 31.070042297986355 31.0 31.0 34.0 28.0 34.0 3 31.160545794749964 31.0 31.0 34.0 28.0 34.0 4 34.80449136132288 37.0 35.0 37.0 32.0 37.0 5 34.83522383611507 37.0 35.0 37.0 32.0 37.0 6 34.90997743445615 37.0 35.0 37.0 32.0 37.0 7 30.968966187283403 37.0 35.0 37.0 2.0 37.0 8 32.73070236381973 37.0 33.0 37.0 17.0 37.0 9 35.58343403603299 39.0 35.0 39.0 30.0 39.0 10-11 36.13135429660985 39.0 36.0 39.0 32.0 39.0 12-13 36.294568375355006 39.0 37.0 39.0 32.0 39.0 14-15 37.511430074939696 40.0 38.0 41.0 32.0 41.0 16-17 37.52488747749807 40.0 38.0 41.0 32.0 41.0 18-19 37.524646840738285 40.0 38.0 41.0 32.0 41.0 20-21 37.59104803869631 40.0 38.0 41.0 32.0 41.0 22-23 37.55711146662115 40.0 38.0 41.0 32.0 41.0 24-25 37.532899860079254 40.0 38.0 41.0 32.0 41.0 26-27 37.55501670594177 40.0 38.0 41.0 32.0 41.0 28-29 37.54000697378794 40.0 38.0 41.0 32.0 41.0 30-31 37.46586449469076 40.0 38.0 41.0 32.0 41.0 32-33 37.417163505318825 40.0 38.0 41.0 31.0 41.0 34-35 37.36362542041547 40.0 37.0 41.0 31.0 41.0 36-37 37.196904252013894 40.0 37.0 41.0 31.0 41.0 38-39 37.09042442550969 40.0 37.0 41.0 30.0 41.0 40-41 37.01927193517024 40.0 37.0 41.0 30.0 41.0 42-43 36.91558014054145 40.0 37.0 41.0 30.0 41.0 44-45 36.81085248966626 40.0 37.0 41.0 30.0 41.0 46-47 36.660693668263804 40.0 36.0 41.0 30.0 41.0 48-49 36.58303229016708 40.0 36.0 41.0 30.0 41.0 50-51 36.45053077577572 40.0 36.0 41.0 30.0 41.0 52-53 36.33581989416775 39.5 35.5 41.0 30.0 41.0 54-55 36.21865689088863 39.0 35.0 41.0 30.0 41.0 56-57 36.023085611348016 39.0 35.0 41.0 29.0 41.0 58-59 35.84822210054098 39.0 35.0 41.0 29.0 41.0 60-61 35.63043271144686 39.0 35.0 41.0 28.5 41.0 62-63 35.40006648603126 38.5 35.0 40.0 28.0 41.0 64-65 35.0483863017553 38.0 34.5 40.0 28.0 41.0 66-67 31.52325915734689 36.5 31.0 40.0 7.0 41.0 68-69 31.895560679656796 36.0 31.0 39.5 11.0 41.0 70-71 32.06216706352525 36.0 31.0 39.0 15.5 41.0 72-73 33.31824930310613 35.5 32.5 39.0 26.0 41.0 74-75 33.39881361171131 35.0 33.0 39.0 26.0 40.0 76-77 33.097718777209266 35.0 33.0 37.5 26.0 39.5 78-79 29.647411922553072 35.0 30.0 37.0 6.5 39.0 80-81 31.482329115096825 35.0 30.5 36.0 21.5 39.0 82-83 31.892580503493342 35.0 32.0 36.0 24.5 38.0 84-85 30.03288040604059 35.0 30.5 36.0 13.0 37.0 86-87 29.660071367226415 35.0 29.0 35.0 13.5 37.0 88-89 30.912188291817884 35.0 30.5 35.0 22.5 36.5 90-91 31.060188855927954 35.0 32.0 35.0 22.0 36.0 92-93 30.911007699805815 35.0 32.0 35.0 20.0 36.0 94-95 30.708459095230886 35.0 32.0 35.0 19.0 36.0 96-97 30.517912947912123 35.0 32.0 35.0 13.5 35.5 98-99 30.334614884769906 35.0 32.0 35.0 2.0 35.0 100 30.13430075624295 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3012080114659206 1101 2 0.31048019982974395 1101 3 0.29692486205414426 1101 4 0.3605600487083862 1101 5 0.36192733067870364 1101 6 0.36296040588445067 1101 7 -12.190077291111688 1101 8 -6.5918714559473095 1101 9 -2.135205200082794 1101 10-11 -0.08558087152119498 1101 12-13 0.38984173725892646 1101 14-15 0.5898577480322515 1101 16-17 0.6462035253778922 1101 18-19 0.6539071311320797 1101 20-21 0.5985401132229526 1101 22-23 0.5658061927379876 1101 24-25 0.5933773101586937 1101 26-27 0.5516278796611616 1101 28-29 0.5236910883641457 1101 30-31 0.6128475950256203 1101 32-33 0.5920045643128589 1101 34-35 0.6189700253729455 1101 36-37 0.6675499155828675 1101 38-39 0.6526071949364507 1101 40-41 0.6348781869133688 1101 42-43 0.6062330668934024 1101 44-45 0.5234963245079456 1101 46-47 0.5739123155729757 1101 48-49 0.4976929713733256 1101 50-51 0.6229798057980602 1101 52-53 0.6418082869132462 1101 54-55 0.5518728059144706 1101 56-57 0.5265592867739244 1101 58-59 0.5023878098256205 1101 60-61 0.520076147664362 1101 62-63 0.5266874050527264 1101 64-65 0.4309151963929665 1101 66-67 -10.3676690939783 1101 68-69 -8.172564866780824 1101 70-71 -6.632687732435983 1101 72-73 -1.361089706720854 1101 74-75 -0.0861341604651713 1101 76-77 0.17485767697246501 1101 78-79 3.3826876074690944 1101 80-81 1.202200840376456 1101 82-83 0.510676806873203 1101 84-85 -5.338359604627158 1101 86-87 -5.856631871552249 1101 88-89 -1.2126301264198993 1101 90-91 0.08369246330407876 1101 92-93 0.4171760988355153 1101 94-95 0.4723750230377739 1101 96-97 0.6075063503845257 1101 98-99 0.6515771601309979 1101 100 0.7427350726810076 1102 1 0.17103879853109305 1102 2 0.22639107707343697 1102 3 0.2060517139940714 1102 4 0.24776902426936687 1102 5 0.25213254563551146 1102 6 0.30305570030015616 1102 7 -12.299459668945484 1102 8 -6.674591143321663 1102 9 -2.346593667139132 1102 10-11 -0.27645129127517265 1102 12-13 0.3281429829701281 1102 14-15 0.48755690515657335 1102 16-17 0.49234997439125294 1102 18-19 0.4840199453393552 1102 20-21 0.4709962177230693 1102 22-23 0.449426361624937 1102 24-25 0.5024690703676171 1102 26-27 0.4584359416185535 1102 28-29 0.45995668606440177 1102 30-31 0.4161152654144189 1102 32-33 0.4445226415529504 1102 34-35 0.4602361197613618 1102 36-37 0.5873776886094788 1102 38-39 0.5680287536234658 1102 40-41 0.44051572787562066 1102 42-43 0.4460241095841724 1102 44-45 0.3922708774896222 1102 46-47 0.4365616660138869 1102 48-49 0.37222819845234767 1102 50-51 0.46614005658174307 1102 52-53 0.45212603466799095 1102 54-55 0.36119447539137184 1102 56-57 0.4231449890152561 1102 58-59 0.37148712850294885 1102 60-61 0.2845163882162538 1102 62-63 0.33139304608867803 1102 64-65 0.26582449632584115 1102 66-67 -10.447686238467348 1102 68-69 -6.8172657918123605 1102 70-71 -6.362509314997531 1102 72-73 -1.1066284399062525 1102 74-75 -0.1954263805831502 1102 76-77 -0.027365566231111416 1102 78-79 3.19539725016476 1102 80-81 1.0097616716388345 1102 82-83 0.36041942540695615 1102 84-85 -5.580349493155964 1102 86-87 -6.039743144817102 1102 88-89 -1.4362985951736071 1102 90-91 -0.10198781513093991 1102 92-93 0.21984900997945545 1102 94-95 0.21456836930242673 1102 96-97 0.2962811757239656 1102 98-99 0.29705720264961144 1102 100 0.38570823488990413 1103 1 0.20237868637074996 1103 2 0.18990294093623916 1103 3 0.21747881080535336 1103 4 0.24622099747328718 1103 5 0.21649313895700573 1103 6 0.30490176223158727 1103 7 -12.404254660993704 1103 8 -6.752535953289577 1103 9 -2.3179943772585645 1103 10-11 -0.3080525182202152 1103 12-13 0.2730484910304014 1103 14-15 0.4552819096649259 1103 16-17 0.4099520550725444 1103 18-19 0.4766430985206256 1103 20-21 0.45409585935719576 1103 22-23 0.4665672092982689 1103 24-25 0.5341051615940486 1103 26-27 0.5243725727069304 1103 28-29 0.4887998909436604 1103 30-31 0.4791680686588222 1103 32-33 0.5012312285679315 1103 34-35 0.5018474735123846 1103 36-37 0.5748227777680057 1103 38-39 0.5888251994501985 1103 40-41 0.5328482481139716 1103 42-43 0.4778501177892025 1103 44-45 0.43577701768440136 1103 46-47 0.47667145189739557 1103 48-49 0.49432395303887233 1103 50-51 0.4539945961798324 1103 52-53 0.39782131237772234 1103 54-55 0.4105267715393808 1103 56-57 0.41378010689547295 1103 58-59 0.39029470670777755 1103 60-61 0.39693415379174013 1103 62-63 0.3379388735652853 1103 64-65 0.2030744264912201 1103 66-67 -10.605322080396007 1103 68-69 -8.463940064584712 1103 70-71 -6.9514528689772135 1103 72-73 -1.6562929284803154 1103 74-75 -0.47469114472418283 1103 76-77 -0.16660633973599204 1103 78-79 3.0688152669276008 1103 80-81 0.9272876287052938 1103 82-83 0.23666303969038083 1103 84-85 -5.706324164970567 1103 86-87 -6.225886342128984 1103 88-89 -1.5724419814448183 1103 90-91 -0.2203525035224203 1103 92-93 0.15213833038774993 1103 94-95 0.23574261809494246 1103 96-97 0.3428188533976311 1103 98-99 0.36031763620277957 1103 100 0.4606802647317494 1104 1 0.1298660245740315 1104 2 0.11682168408781024 1104 3 0.1376332307627841 1104 4 0.14971971307696919 1104 5 0.18436797886165124 1104 6 0.2120343120985524 1104 7 -12.309173569175325 1104 8 -6.700840333366791 1104 9 -2.3803312130654035 1104 10-11 -0.32298794941559805 1104 12-13 0.2373456933070628 1104 14-15 0.4002365197727187 1104 16-17 0.4317041516317701 1104 18-19 0.42171148287791027 1104 20-21 0.44021793885072213 1104 22-23 0.42021800627900774 1104 24-25 0.5308673245689022 1104 26-27 0.4745653064206081 1104 28-29 0.4571177611594379 1104 30-31 0.4296154057828545 1104 32-33 0.470583111349157 1104 34-35 0.4390657016435284 1104 36-37 0.49167287116883074 1104 38-39 0.5354909406359383 1104 40-41 0.4764466387248234 1104 42-43 0.4069720869762534 1104 44-45 0.4278665372304289 1104 46-47 0.41755274501217343 1104 48-49 0.37306553127704944 1104 50-51 0.4082631970942572 1104 52-53 0.3921014910511076 1104 54-55 0.36969671212856525 1104 56-57 0.35827319998774243 1104 58-59 0.32349424323407305 1104 60-61 0.3320889631238444 1104 62-63 0.25489243297522535 1104 64-65 0.1209424762759923 1104 66-67 -10.470376377663591 1104 68-69 -8.403274903753822 1104 70-71 -6.867885498589878 1104 72-73 -1.734664466973804 1104 74-75 -0.5260691026705402 1104 76-77 -0.2224739399231268 1104 78-79 2.9678224560215014 1104 80-81 0.8038833529097928 1104 82-83 0.14249762588869785 1104 84-85 -5.6183023212721395 1104 86-87 -6.075385025914915 1104 88-89 -1.5383559110808385 1104 90-91 -0.2512212121752988 1104 92-93 0.0852729915934809 1104 94-95 0.12136818595347165 1104 96-97 0.25837118567338635 1104 98-99 0.2666881557670635 1104 100 0.3425739150668079 1105 1 0.017711373481727577 1105 2 0.03469863690883557 1105 3 0.02197448912910005 1105 4 0.11667366098172494 1105 5 0.04924366565435179 1105 6 0.1335505856625332 1105 7 -12.399871447517267 1105 8 -6.787812670726165 1105 9 -2.4823736119261355 1105 10-11 -0.4288895665578778 1105 12-13 0.10255285799459557 1105 14-15 0.24801540231185726 1105 16-17 0.2833201911403691 1105 18-19 0.2796771035268151 1105 20-21 0.29949948558872563 1105 22-23 0.2719531353037681 1105 24-25 0.31905303884375513 1105 26-27 0.3002537458464971 1105 28-29 0.2819935158879048 1105 30-31 0.2979500420499548 1105 32-33 0.2726007783804363 1105 34-35 0.30945848908264395 1105 36-37 0.38750347677779473 1105 38-39 0.32725950107018065 1105 40-41 0.3434465121647463 1105 42-43 0.28402421656807064 1105 44-45 0.22248985520235465 1105 46-47 0.25225918668250813 1105 48-49 0.1491796251620201 1105 50-51 0.28537936684476506 1105 52-53 0.26055791882549784 1105 54-55 0.2184090346142824 1105 56-57 0.20927897253343986 1105 58-59 0.19161374162385414 1105 60-61 0.13149048812525876 1105 62-63 0.07903368853384052 1105 64-65 -0.04539050834410574 1105 66-67 -10.649663224342326 1105 68-69 -8.600764031297878 1105 70-71 -6.993278869896621 1105 72-73 -1.8316614140682717 1105 74-75 -0.5691309963912374 1105 76-77 -0.31804581830269285 1105 78-79 2.9227884532151336 1105 80-81 0.7398002474067695 1105 82-83 0.03565980742430597 1105 84-85 -5.7537193670093565 1105 86-87 -6.254765120979393 1105 88-89 -1.6908596389971962 1105 90-91 -0.3901362288893999 1105 92-93 -0.05101859662240571 1105 94-95 -0.018992808729866084 1105 96-97 0.038045535884307924 1105 98-99 0.07570470725012513 1105 100 0.13559068256663664 1106 1 0.12261502822960679 1106 2 0.12013995888473872 1106 3 0.13805932869049187 1106 4 0.14522463876995317 1106 5 0.1475329127472591 1106 6 0.17939023567254964 1106 7 -12.329707338636553 1106 8 -6.752853302334511 1106 9 -2.5412422901217866 1106 10-11 -0.3937200297385388 1106 12-13 0.1860615483193655 1106 14-15 0.31721111252138456 1106 16-17 0.34788612634256566 1106 18-19 0.3334965631214004 1106 20-21 0.32291256565536486 1106 22-23 0.3473345190810093 1106 24-25 0.40589557337879256 1106 26-27 0.3809355980982332 1106 28-29 0.3375499885635733 1106 30-31 0.3112457832693991 1106 32-33 0.34993180013591285 1106 34-35 0.40208921580297385 1106 36-37 0.4503963472696739 1106 38-39 0.36504036228020453 1106 40-41 0.4018880509741791 1106 42-43 0.39410672164746074 1106 44-45 0.27073040064072984 1106 46-47 0.3910821813027212 1106 48-49 0.3065604192262583 1106 50-51 0.35552569065746553 1106 52-53 0.30149667747264175 1106 54-55 0.2717817460121523 1106 56-57 0.32013318408220925 1106 58-59 0.23584255462046855 1106 60-61 0.2557764428390499 1106 62-63 0.19925964604789215 1106 64-65 0.14955251255814517 1106 66-67 -10.5502227748769 1106 68-69 -8.457297602211312 1106 70-71 -6.9053566196862946 1106 72-73 -1.6891714802678948 1106 74-75 -0.47841197838626925 1106 76-77 -0.206247575229213 1106 78-79 2.996297935679735 1106 80-81 0.8449503765139852 1106 82-83 0.15947639037289107 1106 84-85 -5.6578828273821316 1106 86-87 -6.085511966603466 1106 88-89 -1.509260386441877 1106 90-91 -0.2373303400919049 1106 92-93 0.10400421292773387 1106 94-95 0.19617922109105734 1106 96-97 0.25716586798248997 1106 98-99 0.3146513790958778 1106 100 0.42276914760464024 1107 1 0.08827791155401954 1107 2 0.11447046148704842 1107 3 0.07234852624205246 1107 4 0.08709932733060555 1107 5 0.1115377273451088 1107 6 0.1006683633248926 1107 7 -12.283427105033265 1107 8 -6.755360671387038 1107 9 -2.5140824706757527 1107 10-11 -0.4680408808148613 1107 12-13 0.14545398352605332 1107 14-15 0.2597547332302881 1107 16-17 0.30976810354670903 1107 18-19 0.3099628526902194 1107 20-21 0.24904110619728925 1107 22-23 0.32883078229719587 1107 24-25 0.37195375396104424 1107 26-27 0.27080464113436875 1107 28-29 0.2922825619832423 1107 30-31 0.3173918193525367 1107 32-33 0.31168016182816416 1107 34-35 0.2808931985951517 1107 36-37 0.33265521333423465 1107 38-39 0.3540139860520881 1107 40-41 0.35722980469292764 1107 42-43 0.33130296943888027 1107 44-45 0.2977251484934129 1107 46-47 0.30886755741067873 1107 48-49 0.3056046837512696 1107 50-51 0.30724490909629054 1107 52-53 0.3173128129835163 1107 54-55 0.26934592102421107 1107 56-57 0.23585635420617734 1107 58-59 0.2328776365112759 1107 60-61 0.21067819986694047 1107 62-63 0.193137091733945 1107 64-65 0.04971269967712999 1107 66-67 -10.502546953388997 1107 68-69 -8.4552168671886 1107 70-71 -6.952194647275839 1107 72-73 -1.8189424746676295 1107 74-75 -0.5152527155771978 1107 76-77 -0.26930854756434996 1107 78-79 2.976444689439873 1107 80-81 0.8257351557194355 1107 82-83 0.14143564093803818 1107 84-85 -5.65665833483196 1107 86-87 -6.153118283053434 1107 88-89 -1.5748241940695458 1107 90-91 -0.2909810940045823 1107 92-93 0.07766134984175466 1107 94-95 0.1653126810455916 1107 96-97 0.23919512268761522 1107 98-99 0.30036022398106255 1107 100 0.4115311330181619 1108 1 -0.015476178311050859 1108 2 0.007124375822982643 1108 3 0.02452882936384526 1108 4 -0.03649521499055908 1108 5 -0.021977838473702604 1108 6 0.029356245202549758 1108 7 -12.3688502161948 1108 8 -6.826161825891589 1108 9 -2.6816728217506665 1108 10-11 -0.5604578159221063 1108 12-13 0.022391874538648437 1108 14-15 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-0.6353541941324927 2216 3 -0.7082354103607926 2216 4 -0.805657238803569 2216 5 -0.5971631256353902 2216 6 -0.932537235662025 2216 7 3.1903438890945743 2216 8 1.2967211622046761 2216 9 0.038932387823713555 2216 10-11 -0.6682104909824105 2216 12-13 -1.0746767808085664 2216 14-15 -1.231507773367376 2216 16-17 -1.3270244079076647 2216 18-19 -1.8299426388184088 2216 20-21 -1.4242044350522178 2216 22-23 -1.2507122776343635 2216 24-25 -1.2901030709107744 2216 26-27 -1.6614895415701696 2216 28-29 -1.869273287028122 2216 30-31 -1.7655758290897055 2216 32-33 -2.6375268315596543 2216 34-35 -1.7818325677116462 2216 36-37 -1.459096016725681 2216 38-39 -1.711349749676451 2216 40-41 -1.6679268591270926 2216 42-43 -1.39969053256155 2216 44-45 -1.452193213434711 2216 46-47 -1.6725793754042613 2216 48-49 -1.747548437336519 2216 50-51 -2.194666278290839 2216 52-53 -1.988481590375308 2216 54-55 -1.6105794190814393 2216 56-57 -1.6520868013741392 2216 58-59 -1.901669166939989 2216 60-61 -2.0517844588172593 2216 62-63 -1.9335086278826594 2216 64-65 -1.639879666871991 2216 66-67 1.8369020943612782 2216 68-69 1.0359102575592516 2216 70-71 0.664493359833159 2216 72-73 -1.0868252042883668 2216 74-75 -1.8760268957401038 2216 76-77 -1.561230893492116 2216 78-79 1.0652794917200765 2216 80-81 -0.456018751678279 2216 82-83 -1.3116176887954758 2216 84-85 0.4912215038647929 2216 86-87 0.4429060915130272 2216 88-89 -0.9056901354252425 2216 90-91 -1.2002042418538181 2216 92-93 -1.2030749186705627 2216 94-95 -1.199361481501601 2216 96-97 -1.2055430504867068 2216 98-99 -1.2477266683109427 2216 100 -2.37282178695704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103228.0 3 19918.0 4 11855.0 5 11012.0 6 10261.0 7 11493.0 8 12701.0 9 14644.0 10 16629.0 11 19264.0 12 21276.0 13 24243.0 14 28122.0 15 30927.0 16 32567.0 17 33999.0 18 32198.0 19 30625.0 20 29618.0 21 30013.0 22 31888.0 23 36467.0 24 43794.0 25 55048.0 26 69324.0 27 84937.0 28 107442.0 29 136491.0 30 170597.0 31 210683.0 32 281137.0 33 399086.0 34 612163.0 35 862830.0 36 942239.0 37 1613091.0 38 1907441.0 39 673124.0 40 1872.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74974878787654 20.687656454796368 14.689081844653048 22.873512912674045 2 15.285911096942066 20.341697153063812 40.80887328163863 23.563518468355497 3 18.309763373901003 25.19773229993832 30.577928969952723 25.91457535620796 4 12.66106438102419 17.220684245922143 39.0072754101361 31.11097596291757 5 13.113286482209812 37.780025337534276 35.64109974894093 13.465588431314984 6 30.50589948875471 38.06531228409286 17.974211238267916 13.45457698888451 7 26.90539325175777 32.68693539375499 22.654960663856297 17.75271069063094 8 24.447801772748452 36.66171320652414 20.796865080433633 18.093619940293767 9 24.40604423859802 17.580289555965276 22.13044657458878 35.883219630847925 10-11 23.464622802164293 27.40028321885497 29.17706449852452 19.958029480456222 12-13 23.90705099936138 24.852688428338453 29.195274847913343 22.044985724386816 14-15 21.403025268457178 27.291882577020022 26.319397433687115 24.985694720835685 16-17 20.927454880048387 30.544800813169772 27.140174401114802 21.387569905667032 18-19 20.881263387487824 29.532679761307502 28.999022962269322 20.587033888935352 20-21 21.972553142195757 28.736181568624474 28.53447567163654 20.756789617543234 22-23 21.677592116880334 28.593748965969233 28.57436338505332 21.154295532097116 24-25 21.51974379544529 28.66208585860257 28.513693190070978 21.30447715588116 26-27 21.213827182412327 28.702876847303006 28.574331605533608 21.50896436475106 28-29 21.250325327435434 28.712010284511607 28.727516465476157 21.310147922576807 30-31 21.587972313876175 29.049623892346244 28.285222710544637 21.077181083232944 32-33 21.21905900948553 28.87237786124952 28.630360395623423 21.27820273364153 34-35 21.4301476385522 28.584970611263362 28.604162231029225 21.38071951915522 36-37 21.69727285029327 28.790162343760016 28.230467620588296 21.282097185358413 38-39 21.50056077287218 28.89891181138452 28.388285880007786 21.212241535735515 40-41 21.395537049051338 28.70959464168395 28.49605659174788 21.39881171751683 42-43 21.335656560908163 28.708665573436797 28.474515314726517 21.481162550928524 44-45 21.480023662086058 28.79195968294539 28.379835699228163 21.348180955740386 46-47 21.469723198245855 28.91580431462888 28.277083697862448 21.337388789262818 48-49 21.40786133385789 28.687475518265416 28.35331446031446 21.551348687562236 50-51 21.372042861563433 28.694568915234402 28.315732976001506 21.617655247200663 52-53 21.371683882211773 28.752779554097252 28.33496144922415 21.540575114466826 54-55 21.4937977310453 28.92875594520078 28.21494961555015 21.362496708203775 56-57 21.363044434979074 28.71618210134082 28.251405818891573 21.669367644788533 58-59 21.332529077907044 28.493176912384843 28.450646002025966 21.72364800768215 60-61 21.50479614391581 28.773337757430177 28.186473628168656 21.535392470485355 62-63 21.309057367034796 28.817017602002 28.244381595387924 21.629543435575282 64-65 21.32277525379794 28.61717333452607 28.345196235074294 21.714855176601695 66-67 21.259194443519306 28.592216930431963 28.267390494760527 21.88119813128821 68-69 21.738505591958976 28.71361685103408 28.084443410198123 21.463434146808826 70-71 21.408640823005072 28.556751612851695 28.238026558709628 21.796581005433605 72-73 21.309786894942658 28.62872952106587 28.41679742696592 21.644686157025546 74-75 21.33671255599003 28.6734911780917 28.27360293137277 21.716193334545498 76-77 21.343289338542252 28.648380835969878 28.160789366213244 21.84754045927463 78-79 21.50928337051877 28.622950169021877 28.26099610323487 21.60677035722448 80-81 21.309939188970613 28.55286272597747 28.325911790317626 21.81128629473429 82-83 21.22600721925762 28.554247469870887 28.488887560188054 21.730857750683438 84-85 21.340955789544054 28.434192732813806 28.309159518595482 21.915691959046658 86-87 21.434623045859425 28.513613416689005 28.27344612113501 21.778317416316565 88-89 21.301565645740368 28.359411454717424 28.37597336903511 21.9630495305071 90-91 21.24570867960663 28.257207313036382 28.400020264925104 22.09706374243189 92-93 21.354549660341632 28.412382895040945 28.27207973363851 21.96098771097891 94-95 21.31659218920935 28.173571223793232 28.40094308001489 22.10889350698253 96-97 21.305447736985293 28.20951988435353 28.384372527870134 22.10065985079104 98-99 21.319613485282048 28.17654980871384 28.371649996106097 22.132186709898015 100 21.38136829839277 28.138169464665612 28.288129914907543 22.192332322034076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1862.0 1 1541.5 2 1439.0 3 1693.0 4 1655.0 5 1490.5 6 1401.5 7 1448.5 8 1534.5 9 1550.5 10 1444.5 11 1356.0 12 1387.5 13 1516.0 14 1687.5 15 1938.5 16 2351.5 17 3065.5 18 4163.0 19 5767.5 20 8297.0 21 12053.5 22 18193.0 23 27581.5 24 38732.0 25 52400.0 26 71334.0 27 94763.0 28 123098.0 29 151481.5 30 176215.0 31 200821.5 32 223948.0 33 244507.5 34 264581.0 35 282396.0 36 295948.0 37 307218.5 38 314492.0 39 317861.5 40 321330.0 41 319828.5 42 312419.0 43 307918.0 44 302421.5 45 295323.0 46 288495.5 47 279123.0 48 269775.5 49 263780.0 50 256109.5 51 243477.0 52 229669.0 53 218065.5 54 209781.0 55 198492.0 56 180411.5 57 160333.5 58 143088.0 59 127930.0 60 111693.0 61 94147.0 62 78306.5 63 64412.5 64 51987.0 65 41314.5 66 32660.5 67 25186.0 68 18976.5 69 14394.5 70 10700.5 71 7946.0 72 5922.0 73 4146.5 74 2931.5 75 2024.0 76 1281.5 77 837.0 78 566.5 79 404.0 80 286.5 81 197.0 82 151.5 83 110.5 84 79.0 85 68.0 86 56.0 87 46.5 88 45.5 89 38.5 90 30.0 91 26.0 92 22.5 93 16.5 94 14.5 95 18.5 96 21.5 97 17.0 98 14.0 99 23.5 100 43.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.019762108484619387 2 0.0026128884774698842 3 0.0014262491689246092 4 0.0023390486370363592 5 0.0019853388431430564 6 0.0040163176596917 7 11.574034825809907 8 3.422998005419062E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.909548304606203E-4 18-19 0.0 20-21 1.711499002709531E-5 22-23 1.1409993351396875E-5 24-25 0.0 26-27 1.9967488364944528E-4 28-29 0.0 30-31 1.1409993351396875E-5 32-33 3.993497672988906E-5 34-35 5.704996675698437E-5 36-37 5.704996675698437E-5 38-39 3.993497672988906E-5 40-41 1.1409993351396875E-5 42-43 3.422998005419062E-5 44-45 1.7685489694665155E-4 46-47 9.869644248958296E-4 48-49 6.845996010838124E-4 50-51 3.537097938933031E-4 52-53 3.0236482381201715E-4 54-55 3.9934976729889056E-4 56-57 0.0015745790824927685 58-59 3.7652978059609683E-4 60-61 0.0014034291822218156 62-63 0.0024588535672260266 64-65 0.0010268994016257185 66-67 5.787399647682225 68-69 5.441944983978657 70-71 0.0030464682248229655 72-73 0.001357789208816228 74-75 0.003405883015391967 76-77 0.0063667762900794555 78-79 5.41901089734235 80-81 0.005231481951615467 82-83 0.005305646908399546 84-85 5.787616437555902 86-87 0.004061957633097287 88-89 0.001865533912953389 90-91 0.00383375776606935 92-93 0.004193172556638351 94-95 0.0046723922773970195 96-97 0.003377358032013475 98-99 0.006212741379835599 100 0.0068802259908923155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8764247.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.56062090379908 #Duplication Level Percentage of deduplicated Percentage of total 1 90.45036723275545 77.389895814112 2 6.732900219744437 11.521422465693185 3 1.4722459771108163 3.7789883977416547 4 0.5340925954280953 1.8278917633979757 5 0.29235499678878873 1.2507037524788476 6 0.15859733890352495 0.8141812074165508 7 0.09713888714256089 0.5817884448475102 8 0.06761407050266621 0.4628081483233094 9 0.043920734793458205 0.3382096805531438 >10 0.1487767373529793 1.8990940923381636 >50 0.001641232624159446 0.0924204773396934 >100 3.4997685309768104E-4 0.04259575575801396 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1409993351396875E-5 0.0 4 0.0 0.0 0.0 1.1409993351396875E-5 0.0 5 0.0 0.0 0.0 1.1409993351396875E-5 0.0 6 0.0 0.0 0.0 1.1409993351396875E-5 0.0 7 0.0 0.0 0.0 1.1409993351396875E-5 0.0 8 1.1409993351396875E-5 0.0 0.0 2.281998670279375E-5 0.0 9 1.1409993351396875E-5 0.0 0.0 2.281998670279375E-5 0.0 10-11 1.1409993351396875E-5 0.0 0.0 2.281998670279375E-5 0.0 12-13 2.281998670279375E-5 0.0 0.0 3.422998005419062E-5 0.0 14-15 2.281998670279375E-5 0.0 1.1409993351396875E-5 3.422998005419062E-5 0.0 16-17 2.281998670279375E-5 0.0 1.1409993351396875E-5 3.422998005419062E-5 0.0 18-19 2.8524983378492186E-5 0.0 2.281998670279375E-5 3.993497672988906E-5 0.0 20-21 3.422998005419062E-5 0.0 2.281998670279375E-5 7.416495678407969E-5 0.0 22-23 3.422998005419062E-5 0.0 2.281998670279375E-5 1.9396988697374686E-4 0.0 24-25 3.422998005419062E-5 0.0 4.56399734055875E-5 3.651197872447E-4 0.0 26-27 3.993497672988906E-5 0.0 4.56399734055875E-5 6.903045977595108E-4 0.0 28-29 4.56399734055875E-5 0.0 4.56399734055875E-5 0.002287703666955073 0.0 30-31 4.56399734055875E-5 0.0 4.56399734055875E-5 0.007462135651813556 0.0 32-33 5.134497008128593E-5 0.0 4.56399734055875E-5 0.019596663581024132 0.0 34-35 5.704996675698437E-5 0.0 4.56399734055875E-5 0.03911916220526418 0.0 36-37 6.275496343268281E-5 0.0 4.56399734055875E-5 0.07052516890498409 0.0 38-39 6.845996010838124E-5 0.0 4.56399734055875E-5 0.11391166862367069 0.0 40-41 6.845996010838124E-5 0.0 4.56399734055875E-5 0.15923786721209476 0.0 42-43 6.845996010838124E-5 0.0 4.56399734055875E-5 0.20711419931455605 0.0 44-45 6.845996010838124E-5 0.0 4.56399734055875E-5 0.2696808978569408 0.0 46-47 6.845996010838124E-5 0.0 4.56399734055875E-5 0.34629329821489513 0.0 48-49 7.416495678407969E-5 0.0 4.56399734055875E-5 0.4278062907172744 0.0 50-51 7.986995345977812E-5 0.0 4.56399734055875E-5 0.5148873599751354 0.0 52-53 7.986995345977812E-5 0.0 4.56399734055875E-5 0.6016603594125085 0.0 54-55 7.986995345977812E-5 0.0 4.56399734055875E-5 0.7008417266195259 0.0 56-57 7.986995345977812E-5 0.0 4.56399734055875E-5 0.8085064238833068 0.0 58-59 7.986995345977812E-5 0.0 4.56399734055875E-5 0.930051378059062 0.0 60-61 7.986995345977812E-5 0.0 4.56399734055875E-5 1.0573926088573269 0.0 62-63 7.986995345977812E-5 0.0 4.56399734055875E-5 1.1874950580466297 0.0 64-65 8.557495013547655E-5 0.0 4.56399734055875E-5 1.335197421980462 0.0 66-67 9.1279946811175E-5 0.0 4.56399734055875E-5 1.502211199661534 0.0 68-69 9.1279946811175E-5 0.0 4.56399734055875E-5 1.6715697309763178 0.0 70-71 9.1279946811175E-5 0.0 4.56399734055875E-5 1.8785584203640084 0.0 72-73 9.698494348687343E-5 0.0 4.56399734055875E-5 2.1041168739310976 0.0 74-75 1.0268994016257186E-4 0.0 4.56399734055875E-5 2.312269382640631 0.0 76-77 1.0268994016257186E-4 0.0 4.56399734055875E-5 2.5339142084881905 0.0 78-79 1.0268994016257186E-4 0.0 4.56399734055875E-5 2.795037611331584 0.0 80-81 1.0268994016257186E-4 0.0 4.56399734055875E-5 3.1171588386315445 0.0 82-83 1.0268994016257186E-4 0.0 4.56399734055875E-5 3.4488188203732735 0.0 84-85 1.0268994016257186E-4 0.0 4.56399734055875E-5 3.783114510579175 0.0 86-87 1.0268994016257186E-4 0.0 4.56399734055875E-5 4.18577317594997 0.0 88 1.0268994016257186E-4 0.0 4.56399734055875E-5 4.523354944241074 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8230 0.0 42.28281 1 GTATCAA 13260 0.0 36.817947 1 ATCAACG 14015 0.0 33.878387 3 TCAACGC 13995 0.0 33.85347 4 CAACGCA 15210 0.0 31.08192 5 TATCAAC 16460 0.0 29.187954 2 AACGCAG 16735 0.0 28.555086 6 ACGCAGA 19475 0.0 24.037643 7 GTACATG 23055 0.0 23.861519 1 CGCAGAG 19870 0.0 23.428776 8 TACATGG 23075 0.0 22.660896 2 ACATGGG 23450 0.0 21.403955 3 GCAGAGT 22905 0.0 19.356546 9 GTGGTAT 5105 0.0 19.34712 1 CATGGGG 16585 0.0 18.509764 4 AGAGTAC 19560 0.0 17.323877 10-11 CAGAGTA 21780 0.0 16.253937 10-11 TGGTATC 5475 0.0 16.073566 2 GTACCGT 525 2.7284841E-11 15.592759 6 GAGTACA 17760 0.0 15.473412 1 >>END_MODULE