FastQCFastQC Report
Wed 25 May 2016
SRR2049535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764247
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232970.265818615107493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231020.2635936664039706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133340.15214085134752592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161050.057.4499471
GTATCAA279400.039.384031
GTGGTAT61950.034.4306071
ATCAACG350200.031.0666163
TCAACGC353050.030.8824734
TATCAAC362150.030.1324562
CAACGCA366300.029.8566655
TGGTATC71250.029.1917212
AACGCAG385500.028.4424636
ACGCAGA435150.025.1211857
CGCAGAG434950.024.9814648
GCAGAGT468350.022.6580669
GTACATG226250.021.8518661
GAGTACT336600.021.14582312-13
CAGAGTA455550.020.79295210-11
TACATGG228500.020.6555632
AGAGTAC432900.020.11129610-11
ACATGGG231850.019.6264463
GTACTTT364850.019.32818414-15
AGTACTT353050.018.95584712-13
Produced by FastQC (version 0.11.5)