##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049535_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8764247 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7207432652229 31.0 31.0 34.0 30.0 34.0 2 31.863001693128915 31.0 31.0 34.0 30.0 34.0 3 31.982332309894964 33.0 31.0 34.0 30.0 34.0 4 35.657438568310546 37.0 35.0 37.0 33.0 37.0 5 35.730381514806695 37.0 35.0 37.0 33.0 37.0 6 35.832423709646704 37.0 35.0 37.0 35.0 37.0 7 35.82610611042797 37.0 35.0 37.0 35.0 37.0 8 35.82705359627587 37.0 35.0 37.0 35.0 37.0 9 37.56725272576184 39.0 37.0 39.0 35.0 39.0 10-11 37.521067468773985 39.0 37.0 39.0 35.0 39.0 12-13 37.4999555580759 39.0 37.0 39.0 35.0 39.0 14-15 38.78648713346395 40.0 38.0 41.0 35.0 41.0 16-17 38.79386694601373 40.0 38.0 41.0 35.0 41.0 18-19 38.86593075252215 40.0 38.0 41.0 35.0 41.0 20-21 38.894283787300836 40.0 38.0 41.0 35.0 41.0 22-23 38.87306639121421 40.0 38.0 41.0 35.0 41.0 24-25 38.837162508085406 40.0 38.0 41.0 35.0 41.0 26-27 38.75126237313941 40.0 38.0 41.0 35.0 41.0 28-29 38.66985914477307 40.0 38.0 41.0 34.0 41.0 30-31 38.563861219338065 40.0 38.0 41.0 34.0 41.0 32-33 38.43239481954354 40.0 38.0 41.0 34.0 41.0 34-35 38.33390352873441 40.0 38.0 41.0 34.0 41.0 36-37 38.20194210637834 40.0 38.0 41.0 34.0 41.0 38-39 38.11722341919391 40.0 38.0 41.0 33.5 41.0 40-41 37.998625666300825 40.0 38.0 41.0 33.0 41.0 42-43 37.86222056498408 40.0 37.0 41.0 33.0 41.0 44-45 37.690145656552126 40.0 37.0 41.0 33.0 41.0 46-47 37.46277700753984 40.0 37.0 41.0 33.0 41.0 48-49 37.24509538583292 40.0 37.0 41.0 32.0 41.0 50-51 37.04494379266125 40.0 37.0 41.0 31.0 41.0 52-53 36.94800677114645 40.0 36.5 41.0 31.0 41.0 54-55 36.82252052001729 40.0 36.0 41.0 31.0 41.0 56-57 36.65961844754033 39.5 36.0 41.0 31.0 41.0 58-59 36.49574989157654 39.0 35.0 41.0 31.0 41.0 60-61 36.31311469199807 39.0 35.0 41.0 31.0 41.0 62-63 36.12299385218148 39.0 35.0 41.0 30.0 41.0 64-65 35.85397764348723 38.5 35.0 40.5 30.0 41.0 66-67 35.60554009945179 38.0 35.0 40.0 30.0 41.0 68-69 35.30429876063511 37.0 35.0 40.0 29.0 41.0 70-71 35.01816830356333 37.0 34.5 39.5 29.0 41.0 72-73 34.70043136620865 36.0 34.0 39.0 29.0 41.0 74-75 34.36414006816558 36.0 34.0 39.0 29.0 41.0 76-77 34.06922933596006 35.5 34.0 38.5 29.0 40.0 78-79 33.74071252213682 35.0 34.0 37.0 29.0 39.0 80-81 33.42828117464056 35.0 34.0 37.0 28.0 39.0 82-83 33.12663546565952 35.0 34.0 36.5 28.0 39.0 84-85 32.84640928079732 35.0 34.0 36.0 27.5 37.5 86-87 32.56429183248715 35.0 33.0 36.0 27.0 37.0 88-89 32.352641019816076 35.0 33.0 35.5 27.0 37.0 90-91 32.20156894254578 35.0 33.0 35.0 27.0 36.0 92-93 32.04199556447919 35.0 33.0 35.0 26.5 36.0 94-95 31.85867433905046 35.0 33.0 35.0 26.0 36.0 96-97 31.695075058929763 35.0 33.0 35.0 26.0 36.0 98-99 31.519725083056194 35.0 33.0 35.0 25.0 35.5 100 31.410271869334583 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35394293533257226 1101 2 0.366695375772224 1101 3 0.45996973104428207 1101 4 0.3463836939077254 1101 5 0.2951625659484094 1101 6 0.2794972986591091 1101 7 0.28469932712594925 1101 8 0.3003776784647201 1101 9 0.3101481014608254 1101 10-11 0.32746122986474546 1101 12-13 0.29703089426610774 1101 14-15 0.44860798661974144 1101 16-17 0.4672373313347009 1101 18-19 0.44408291752294815 1101 20-21 0.4294670250979493 1101 22-23 0.45401932058494054 1101 24-25 0.48462682306990246 1101 26-27 0.5233704979890064 1101 28-29 0.46937658049249364 1101 30-31 0.47637832695274085 1101 32-33 0.4931527209869344 1101 34-35 0.5292047259324093 1101 36-37 0.5211562903594498 1101 38-39 0.5364994992533241 1101 40-41 0.5224092150623889 1101 42-43 0.440679941239118 1101 44-45 0.45837177566489373 1101 46-47 0.5644654144797983 1101 48-49 0.5757360363971031 1101 50-51 0.635179535435654 1101 52-53 0.6151815666444946 1101 54-55 0.513532361883982 1101 56-57 0.5316474361222348 1101 58-59 0.5185840901124266 1101 60-61 0.5675538981677946 1101 62-63 0.5419654031281524 1101 64-65 0.5529176977745394 1101 66-67 0.5316136799710662 1101 68-69 0.5177364867257026 1101 70-71 0.5289119834266813 1101 72-73 0.4926080434979667 1101 74-75 0.531389768061679 1101 76-77 0.5078150408230684 1101 78-79 0.4385251093363536 1101 80-81 0.46764071887643155 1101 82-83 0.5184934811154562 1101 84-85 0.5228971175157753 1101 86-87 0.5605218022935361 1101 88-89 0.5448804179324114 1101 90-91 0.5461896353422091 1101 92-93 0.54762383508249 1101 94-95 0.48121136328035874 1101 96-97 0.5238117738957051 1101 98-99 0.584897349254053 1101 100 0.6359918796145578 1102 1 0.23516489006506092 1102 2 0.31906429863251873 1102 3 0.36747264305001437 1102 4 0.34603116893035235 1102 5 0.2603261433336215 1102 6 0.23876263493104233 1102 7 0.272461519942091 1102 8 0.27795723040566145 1102 9 0.3341907331788079 1102 10-11 0.28796882016901293 1102 12-13 0.28999392479313 1102 14-15 0.35884600690734203 1102 16-17 0.4188055750514579 1102 18-19 0.40820990746558294 1102 20-21 0.38042700989238654 1102 22-23 0.38263074977326283 1102 24-25 0.3793265761254361 1102 26-27 0.3521825640648899 1102 28-29 0.3860513665548595 1102 30-31 0.4624303129440932 1102 32-33 0.49841047890403445 1102 34-35 0.4664821611036629 1102 36-37 0.48803287447753263 1102 38-39 0.4812212347959033 1102 40-41 0.4442460420392962 1102 42-43 0.4658403374694089 1102 44-45 0.49901445785282306 1102 46-47 0.5121221663375692 1102 48-49 0.532711105272476 1102 50-51 0.5730940797280866 1102 52-53 0.46852699139271436 1102 54-55 0.4334603473023506 1102 56-57 0.49037112021170515 1102 58-59 0.5052941896017771 1102 60-61 0.493134404901042 1102 62-63 0.4723378225163657 1102 64-65 0.473540391388255 1102 66-67 0.4869822770963381 1102 68-69 0.4919575945147088 1102 70-71 0.4733948655316951 1102 72-73 0.5131512967279974 1102 74-75 0.5101479799826905 1102 76-77 0.45603416553411336 1102 78-79 0.45469757432557856 1102 80-81 0.4096609382792593 1102 82-83 0.4216503987588567 1102 84-85 0.4165614990116211 1102 86-87 0.4365769606584351 1102 88-89 0.4018381598012084 1102 90-91 0.416231438881816 1102 92-93 0.41544945226767993 1102 94-95 0.43956993462715843 1102 96-97 0.4434662363270725 1102 98-99 0.4666351321675357 1102 100 0.5172266956859914 1103 1 0.24299924662172145 1103 2 0.28310252348484255 1103 3 0.35802678478845706 1103 4 0.3113155484121535 1103 5 0.2184549544391956 1103 6 0.21957820498425207 1103 7 0.2556311311585304 1103 8 0.24989561336551702 1103 9 0.2786331880514936 1103 10-11 0.27857023387528557 1103 12-13 0.2429473841493106 1103 14-15 0.3458792129356496 1103 16-17 0.34240384294484727 1103 18-19 0.3587186058997247 1103 20-21 0.3453737049159713 1103 22-23 0.3390085120677995 1103 24-25 0.3356434151047978 1103 26-27 0.31444264308151304 1103 28-29 0.32778750287572933 1103 30-31 0.35661011370872586 1103 32-33 0.3197632563406998 1103 34-35 0.32734851275498755 1103 36-37 0.35932505702832884 1103 38-39 0.3727607210160073 1103 40-41 0.39394325014209386 1103 42-43 0.38575564988364164 1103 44-45 0.4008375091657328 1103 46-47 0.43793485437539914 1103 48-49 0.4215099260480102 1103 50-51 0.4659937292522329 1103 52-53 0.42158921483804335 1103 54-55 0.405242151107835 1103 56-57 0.4185269617562781 1103 58-59 0.3817720009756087 1103 60-61 0.399066887753726 1103 62-63 0.40112981106249634 1103 64-65 0.3943035880859469 1103 66-67 0.4121077123471224 1103 68-69 0.43002424674248374 1103 70-71 0.43163104281302367 1103 72-73 0.42346741424265133 1103 74-75 0.382565357484566 1103 76-77 0.4238511109669858 1103 78-79 0.3418511281639951 1103 80-81 0.33130370851091584 1103 82-83 0.33693699819139056 1103 84-85 0.33317086194116285 1103 86-87 0.3554061361431522 1103 88-89 0.3809616569205829 1103 90-91 0.3544847687854329 1103 92-93 0.3359204786390819 1103 94-95 0.35541662814550534 1103 96-97 0.3537543537971466 1103 98-99 0.41021265142695995 1103 100 0.40351754093178727 1104 1 0.22395759737789334 1104 2 0.2677321238263488 1104 3 0.3227662501657278 1104 4 0.2602653550701888 1104 5 0.19162848768492324 1104 6 0.23425676489501512 1104 7 0.18902786226728097 1104 8 0.2096890874164714 1104 9 0.26145454687778624 1104 10-11 0.236694905386436 1104 12-13 0.24796866951488994 1104 14-15 0.3191599224205035 1104 16-17 0.3040294555839296 1104 18-19 0.3331517975278402 1104 20-21 0.3245992739136625 1104 22-23 0.34032754947467225 1104 24-25 0.33764160873528226 1104 26-27 0.34998376806891685 1104 28-29 0.340650146097353 1104 30-31 0.37963763636071235 1104 32-33 0.3988012859467247 1104 34-35 0.3360469797775565 1104 36-37 0.3195109960802469 1104 38-39 0.39908077666466824 1104 40-41 0.4142509378909054 1104 42-43 0.35874601929267413 1104 44-45 0.34532982584096317 1104 46-47 0.3833031934905975 1104 48-49 0.4403646306417741 1104 50-51 0.48468209953901464 1104 52-53 0.4523692247496669 1104 54-55 0.41608155587916684 1104 56-57 0.4298795106726345 1104 58-59 0.43803934238816566 1104 60-61 0.4190787667502107 1104 62-63 0.41588857209094243 1104 64-65 0.4063158030101661 1104 66-67 0.4099539468386695 1104 68-69 0.3534496318747955 1104 70-71 0.43947094603795733 1104 72-73 0.4331040784447424 1104 74-75 0.4594494282450938 1104 76-77 0.46851000807033216 1104 78-79 0.40496590621944506 1104 80-81 0.4090276967849036 1104 82-83 0.4105898913139896 1104 84-85 0.447605289944363 1104 86-87 0.4682750496228181 1104 88-89 0.43193427300480636 1104 90-91 0.4406832998854213 1104 92-93 0.4541207655586277 1104 94-95 0.46957642240640496 1104 96-97 0.46695981183826873 1104 98-99 0.5209733931842457 1104 100 0.5200129633488082 1105 1 0.14946435551493664 1105 2 0.21954984410881906 1105 3 0.31514232984574875 1105 4 0.249075709119154 1105 5 0.18341607369968926 1105 6 0.20493800562273634 1105 7 0.2191476936136212 1105 8 0.1996410475584085 1105 9 0.27556485519599505 1105 10-11 0.22176432304190996 1105 12-13 0.23865382830788207 1105 14-15 0.3025187094998998 1105 16-17 0.2693808050497353 1105 18-19 0.27973106667855774 1105 20-21 0.23995742170789214 1105 22-23 0.2900240760846984 1105 24-25 0.2720688539604339 1105 26-27 0.2912942228338551 1105 28-29 0.3083628932766871 1105 30-31 0.3051372327366835 1105 32-33 0.32740153589813303 1105 34-35 0.3292059123915365 1105 36-37 0.3696073395819681 1105 38-39 0.33037954374434975 1105 40-41 0.3262284345686979 1105 42-43 0.30817695205278994 1105 44-45 0.36103039845404794 1105 46-47 0.31397987610775857 1105 48-49 0.37249248736493 1105 50-51 0.4283302576338315 1105 52-53 0.34596814306483026 1105 54-55 0.3080115997732733 1105 56-57 0.3212871349765791 1105 58-59 0.3744349228190913 1105 60-61 0.3627498620773153 1105 62-63 0.34270158556426367 1105 64-65 0.33671256528606364 1105 66-67 0.35059404948439976 1105 68-69 0.3598743402450353 1105 70-71 0.348734064008859 1105 72-73 0.32048095256953957 1105 74-75 0.3327814297442373 1105 76-77 0.30264651073750315 1105 78-79 0.26332608586349693 1105 80-81 0.25679127298974436 1105 82-83 0.26547582702621497 1105 84-85 0.30616485277906236 1105 86-87 0.37364453604070036 1105 88-89 0.3310881465650226 1105 90-91 0.3275644952244434 1105 92-93 0.32878363426699053 1105 94-95 0.3221029111833147 1105 96-97 0.3202231425004136 1105 98-99 0.3914949141693711 1105 100 0.41216467443723914 1106 1 0.15625096225245372 1106 2 0.15535238505335514 1106 3 0.25419338847414963 1106 4 0.25242115714151225 1106 5 0.17247373330246774 1106 6 0.19563776066978278 1106 7 0.1927560727374953 1106 8 0.23731152797122945 1106 9 0.20285499076356217 1106 10-11 0.21051937309078994 1106 12-13 0.15866287956761482 1106 14-15 0.2683042411397807 1106 16-17 0.29360656996284007 1106 18-19 0.2429885648483463 1106 20-21 0.24759745690148804 1106 22-23 0.22754546426089917 1106 24-25 0.2172571152612619 1106 26-27 0.2372553930716279 1106 28-29 0.24452772624902508 1106 30-31 0.29110141567107206 1106 32-33 0.2759602937660759 1106 34-35 0.26792591165585833 1106 36-37 0.2934433526887261 1106 38-39 0.28126270865090675 1106 40-41 0.3011418832431829 1106 42-43 0.27955803484666575 1106 44-45 0.3274170235927514 1106 46-47 0.35140213572022816 1106 48-49 0.34867015318572925 1106 50-51 0.4234712150055344 1106 52-53 0.3608035065871533 1106 54-55 0.30510480619566493 1106 56-57 0.36384360760643375 1106 58-59 0.3419871112287609 1106 60-61 0.35541432316650656 1106 62-63 0.3491431929029005 1106 64-65 0.34414717607285183 1106 66-67 0.33225556008463286 1106 68-69 0.34477341748516466 1106 70-71 0.38774243274798437 1106 72-73 0.3676587886597389 1106 74-75 0.36473797320745405 1106 76-77 0.3251566321361068 1106 78-79 0.32144796012060795 1106 80-81 0.2761636239679959 1106 82-83 0.29725471735753217 1106 84-85 0.2861533043950075 1106 86-87 0.3465437484610874 1106 88-89 0.35570046758373763 1106 90-91 0.35116113482917655 1106 92-93 0.38038933388083507 1106 94-95 0.35405012693378524 1106 96-97 0.3791354330497434 1106 98-99 0.4527263180369765 1106 100 0.4926035366225676 1107 1 0.12017718797749666 1107 2 0.1575766854431322 1107 3 0.2184704173472909 1107 4 0.1866039250933298 1107 5 0.1587863951096935 1107 6 0.1503223411600274 1107 7 0.1279556459669564 1107 8 0.1462597207345837 1107 9 0.1918825202204104 1107 10-11 0.11709930054929885 1107 12-13 0.11472722695133086 1107 14-15 0.19940391356147558 1107 16-17 0.1905382621483085 1107 18-19 0.17226519961176479 1107 20-21 0.19890774073218154 1107 22-23 0.19944031423584363 1107 24-25 0.2193917309971951 1107 26-27 0.20237601206383005 1107 28-29 0.23172429922811233 1107 30-31 0.23013871685127185 1107 32-33 0.24052505824462145 1107 34-35 0.21092990322920713 1107 36-37 0.29887131221904184 1107 38-39 0.26565045083332706 1107 40-41 0.21651556539790562 1107 42-43 0.2777421724046505 1107 44-45 0.307935154448586 1107 46-47 0.3166689865075014 1107 48-49 0.3025271781952341 1107 50-51 0.3898955896579679 1107 52-53 0.364149893153801 1107 54-55 0.3675705574401391 1107 56-57 0.3380643153049476 1107 58-59 0.31382445681205695 1107 60-61 0.2983745115152132 1107 62-63 0.3401777241277628 1107 64-65 0.35796527189942395 1107 66-67 0.30813747740096886 1107 68-69 0.37992892223449104 1107 70-71 0.3533706608322831 1107 72-73 0.364568967404864 1107 74-75 0.38897496026744705 1107 76-77 0.32904802715285797 1107 78-79 0.3082613304929396 1107 80-81 0.3244651958986111 1107 82-83 0.35721596020221114 1107 84-85 0.31814962746570785 1107 86-87 0.36426691272501444 1107 88-89 0.34269435596427655 1107 90-91 0.2958007288777438 1107 92-93 0.288264767998065 1107 94-95 0.32560446212174554 1107 96-97 0.31068633667527834 1107 98-99 0.35775379339946056 1107 100 0.31559781063351977 1108 1 0.05928184420484328 1108 2 0.08752526602556188 1108 3 0.16087177256768825 1108 4 0.1379198640763235 1108 5 0.11299135358894574 1108 6 0.14084838658664722 1108 7 0.10650607183308836 1108 8 0.15171663785069 1108 9 0.14322251735206493 1108 10-11 0.11554386528209193 1108 12-13 0.08656259849450976 1108 14-15 0.07383933693039779 1108 16-17 0.13851740971293225 1108 18-19 0.16396081528998963 1108 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-0.6104434274354773 2215 20-21 -0.6683923235623581 2215 22-23 -0.6397468561657647 2215 24-25 -0.595356555447907 2215 26-27 -0.5540273756265321 2215 28-29 -0.5446514324437288 2215 30-31 -0.5157655573149356 2215 32-33 -0.6065906503694052 2215 34-35 -0.6390602919683559 2215 36-37 -0.7005058603783283 2215 38-39 -0.6579380089908753 2215 40-41 -0.6148462474785674 2215 42-43 -0.6074207773366354 2215 44-45 -0.6950460387809798 2215 46-47 -0.6835810977321515 2215 48-49 -0.7077826152743256 2215 50-51 -0.6600736480612994 2215 52-53 -0.7398777494445952 2215 54-55 -0.6697301669216884 2215 56-57 -0.6591711792109081 2215 58-59 -0.6827846062048479 2215 60-61 -0.8128874168105256 2215 62-63 -0.7840628578581956 2215 64-65 -0.7103605514185318 2215 66-67 -0.5935058962711182 2215 68-69 -0.5987592943366593 2215 70-71 -0.6503679713326846 2215 72-73 -0.5775956129018738 2215 74-75 -0.6543956263664299 2215 76-77 -0.6165155383087253 2215 78-79 -0.588092531248904 2215 80-81 -0.6245447842348071 2215 82-83 -0.6057397166113816 2215 84-85 -0.5525598826535045 2215 86-87 -0.5406676723894321 2215 88-89 -0.536173800243823 2215 90-91 -0.5492285302799402 2215 92-93 -0.7122669802764925 2215 94-95 -0.738868953375146 2215 96-97 -0.820760989979437 2215 98-99 -0.7920284014875847 2215 100 -0.846269153654017 2216 1 -0.5141094667811572 2216 2 -0.9363130233016506 2216 3 -0.6218610494801098 2216 4 -1.072988455756601 2216 5 -0.504182399557827 2216 6 -0.773452242573768 2216 7 -0.48957674161032116 2216 8 -0.5081372152790067 2216 9 -0.5308251676002982 2216 10-11 -0.7880969761002916 2216 12-13 -0.5874618555064899 2216 14-15 -0.853012695282942 2216 16-17 -0.7828505402852741 2216 18-19 -0.833629969408257 2216 20-21 -0.6449777652208795 2216 22-23 -0.6535334896244294 2216 24-25 -0.757746303745293 2216 26-27 -0.8475775190518178 2216 28-29 -1.0929416523912394 2216 30-31 -1.2347972554429418 2216 32-33 -0.9306168468523666 2216 34-35 -0.9370385696186645 2216 36-37 -0.9160161067707335 2216 38-39 -1.0648207136017191 2216 40-41 -0.9325067021758358 2216 42-43 -1.481226690598099 2216 44-45 -1.1661470263132756 2216 46-47 -1.050000519572805 2216 48-49 -0.9580065071135948 2216 50-51 -1.1161415457570598 2216 52-53 -1.0118061828062608 2216 54-55 -1.2471115580030272 2216 56-57 -1.2759124101591723 2216 58-59 -1.081401033406813 2216 60-61 -1.3073822709418508 2216 62-63 -1.2392047217710953 2216 64-65 -1.2539235097208206 2216 66-67 -1.102712768148038 2216 68-69 -1.3921481600934626 2216 70-71 -1.1965241135060012 2216 72-73 -1.0183816085463206 2216 74-75 -1.1867053353926238 2216 76-77 -1.2880319250079495 2216 78-79 -1.2812959507187287 2216 80-81 -1.0623824276845042 2216 82-83 -1.1526500110720121 2216 84-85 -1.2260197408021227 2216 86-87 -1.4263627789563316 2216 88-89 -1.2192278463380966 2216 90-91 -1.0950693444858146 2216 92-93 -1.1303451428453428 2216 94-95 -1.221891567551829 2216 96-97 -1.321817148658237 2216 98-99 -1.3513359747132299 2216 100 -1.3606496659167036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 632.0 3 54.0 4 73.0 5 135.0 6 422.0 7 1101.0 8 2528.0 9 4526.0 10 6522.0 11 9247.0 12 12868.0 13 18611.0 14 22684.0 15 29695.0 16 37940.0 17 53612.0 18 53475.0 19 29362.0 20 21824.0 21 21116.0 22 22494.0 23 24423.0 24 28516.0 25 35602.0 26 45563.0 27 59292.0 28 78149.0 29 103019.0 30 133805.0 31 173825.0 32 224535.0 33 293810.0 34 397680.0 35 576167.0 36 946037.0 37 1743227.0 38 2505985.0 39 1041944.0 40 3747.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.632275708249324 21.08552696549514 15.200992073662977 23.081205252592554 2 15.277400612946732 20.746854825643354 39.839164238232264 24.136580323177647 3 17.676101286978938 25.241395551882047 31.368448580744996 25.714054580394023 4 12.655852863861167 17.681037103328663 38.2640393095985 31.399070723211665 5 12.567628319230312 37.71424952761727 35.72014404250075 13.99797811065166 6 30.08980966649133 38.530902809076586 17.411585230183626 13.967702294248463 7 26.052460639231185 33.274564260911404 22.546181092340277 18.126794007517134 8 24.55020950459292 36.56241089508317 20.340617967521908 18.546761632801996 9 24.321872408147517 17.022462750062957 22.069527282618704 36.58613755917083 10-11 23.472872227357353 27.360559327002083 28.785462116711223 20.38110632892934 12-13 24.28205876730162 24.798264128756333 29.235680268846902 21.68399683509514 14-15 21.44883354735637 26.890792114573266 27.06015071352137 24.600223624549 16-17 20.351543036155874 30.22429080330575 27.863232289094547 21.560933871443833 18-19 20.206522020659616 28.44954050245275 30.867797313334506 20.47614016355313 20-21 21.23170421828595 27.59839493341527 30.773847428079105 20.396053420219673 22-23 20.926207351298977 27.472742381633015 30.87579571867384 20.725254548394176 24-25 20.84460878384646 27.545064624490845 30.7946706659454 20.81565592571729 26-27 20.606955737326892 27.609211607112393 30.820520005882994 20.963312649677718 28-29 20.605059187434932 27.587563704230384 30.999225431408234 20.808151676926453 30-31 20.9413280091125 27.88066417057208 30.596660320602236 20.581347499713182 32-33 20.61911879023948 27.699852594295894 30.900304384392633 20.78072423107199 34-35 20.823528821129756 27.480010547397853 30.80260061132463 20.893860020147766 36-37 21.081212110977702 27.691859893953243 30.378605258386713 20.84832273668234 38-39 20.936145455508044 27.788673687539845 30.477398685819786 20.79778217113233 40-41 20.906194222960625 27.646294085504437 30.46962848034748 20.977883211187454 42-43 20.883431286224592 27.70967659857145 30.300252834042674 21.10663928116129 44-45 21.06887220316817 27.827484779924617 30.079138572885956 21.02450444402126 46-47 21.133612505443995 28.023805125528757 29.718502913028356 21.124079455998903 48-49 21.198261527773006 27.983932903762298 29.42922535159039 21.38858021687431 50-51 21.251603246690788 28.02608141920236 29.227622179064554 21.4946931550423 52-53 21.21142866010052 28.157478902637045 29.205281412082524 21.425811025179918 54-55 21.33474216324574 28.351682694474494 29.073758418720967 21.239816723558796 56-57 21.163694952914952 28.132793382021298 29.098483874313448 21.605027790750306 58-59 21.099262194421346 27.84662959097505 29.42449156703159 21.62961664757201 60-61 21.253982230304555 28.067767829911684 29.165141055472304 21.513108884311453 62-63 21.047894930391625 28.09476387418109 29.266262121549065 21.591079073878223 64-65 21.044112517595636 27.905945599205502 29.3805103849766 21.669431498222266 66-67 20.99589388569263 27.93918861483479 29.2612759544545 21.80364154501807 68-69 21.32781059228477 28.06294710772072 29.036590365378796 21.572651934615717 70-71 21.22927959469878 27.901079237041127 29.02912822972698 21.84051293853311 72-73 21.085399578537668 27.98259793454018 29.18712811266045 21.744874374261702 74-75 21.160397464836397 28.026651918869927 28.977406729865095 21.835543886428578 76-77 21.146648422847964 28.008994954158638 28.881933610497285 21.96242301249611 78-79 21.21769050442888 27.98369190888632 28.993072244667918 21.80554534201688 80-81 21.091059640997265 27.93078747539856 28.983270964237217 21.994881919366954 82-83 20.97027579010316 27.918321762708022 29.123694357495268 21.987708089693555 84-85 21.036176867219737 27.813946822813186 28.952019494658245 22.197856815308832 86-87 21.088682357843446 27.885188298000763 28.87887902735729 22.1472503167985 88-89 20.94599879430497 27.76471364115506 28.930935764592007 22.358351799947958 90-91 20.84051450445384 27.71663346926071 28.916515170341654 22.526336855943796 92-93 20.890924978921312 27.820709139647747 28.811953788963912 22.476412092467026 94-95 20.765740077688886 27.607571859480878 28.95658445482913 22.670103608001106 96-97 20.749691416694603 27.62068180194851 28.914455390686207 22.715171390670687 98-99 20.73676883504408 27.56813581643089 28.848417743851535 22.84667760467349 100 20.7643354835294 27.52451403796507 28.758060382726836 22.953090095778695 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 950.0 1 758.0 2 918.0 3 1338.5 4 959.5 5 662.5 6 1687.5 7 4494.0 8 7617.0 9 8945.0 10 7418.0 11 5329.5 12 5145.0 13 5851.0 14 6611.5 15 7374.0 16 8323.5 17 9456.5 18 10720.0 19 12284.5 20 14537.5 21 17587.0 22 22562.5 23 30689.5 24 40820.0 25 52537.5 26 69666.5 27 91625.5 28 118728.5 29 146655.0 30 169972.0 31 193412.0 32 215726.5 33 235940.5 34 255900.5 35 273806.0 36 287326.5 37 297652.5 38 305666.5 39 311077.0 40 315989.0 41 315392.0 42 308746.0 43 305426.5 44 301299.5 45 294628.0 46 287877.5 47 278461.0 48 269008.5 49 264750.5 50 258113.0 51 244335.5 52 229419.5 53 217518.0 54 210033.0 55 199361.5 56 182125.0 57 162377.0 58 145583.5 59 132153.0 60 115599.0 61 96856.5 62 81230.0 63 66766.5 64 53157.0 65 42360.5 66 33411.0 67 25390.0 68 19029.5 69 14476.5 70 10733.5 71 7802.0 72 5721.0 73 3849.0 74 2650.0 75 1828.5 76 1084.0 77 615.5 78 364.0 79 201.0 80 102.0 81 53.0 82 34.5 83 21.0 84 10.0 85 5.0 86 3.0 87 4.0 88 5.5 89 3.5 90 1.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05956016529429168 2 3.080698204877156E-4 3 1.1409993351396875E-5 4 0.1204667098040482 5 0.0019054688896832778 6 0.001791368956169309 7 0.0 8 0.0 9 4.56399734055875E-5 10-11 0.0 12-13 2.8524983378492186E-5 14-15 3.993497672988906E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.540349102438578E-4 30-31 2.5672485040642963E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5973990691955624E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.698494348687343E-5 80-81 2.7383984043352497E-4 82-83 3.1377481716341404E-4 84-85 0.0 86-87 1.1409993351396875E-5 88-89 8.557495013547655E-5 90-91 9.1279946811175E-5 92-93 7.245345778137015E-4 94-95 6.731896077324156E-4 96-97 1.1409993351396875E-5 98-99 6.275496343268281E-5 100 9.698494348687343E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8764247.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.56035915088498 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8424607119587 39.19750458727809 2 16.152823807319464 17.626077364566306 3 5.266496344415147 8.620257960543396 4 2.3576141268242323 5.145290939949208 5 1.2439283534748062 3.393458886177722 6 0.7483880385277604 2.4499392099780573 7 0.4746070868910095 1.8126313181430125 8 0.3362507672436845 1.4676770100460848 9 0.24740630059763738 1.2148718955119016 >10 1.23040286192714 11.883397481375383 >50 0.06781072912129567 2.523520509303947 >100 0.029572222400284967 2.85342299805757 >500 0.0015485802692351285 0.5452534597002547 >1k 6.064243435835862E-4 0.5047779082135446 >5k 2.0911171497538684E-5 0.08041211399504448 >10k+ 6.273351449261604E-5 0.681506357160328 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23297 0.265818615107493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23102 0.2635936664039706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13334 0.15214085134752592 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.1409993351396875E-5 0.0 6 0.0 0.0 0.0 1.1409993351396875E-5 0.0 7 0.0 0.0 0.0 2.281998670279375E-5 0.0 8 0.0 0.0 0.0 4.56399734055875E-5 0.0 9 2.281998670279375E-5 0.0 0.0 4.56399734055875E-5 0.0 10-11 3.422998005419062E-5 0.0 0.0 4.56399734055875E-5 0.0 12-13 3.422998005419062E-5 1.1409993351396875E-5 0.0 6.845996010838124E-5 0.0 14-15 3.422998005419062E-5 1.1409993351396875E-5 0.0 6.845996010838124E-5 0.0 16-17 3.422998005419062E-5 1.1409993351396875E-5 0.0 6.845996010838124E-5 0.0 18-19 3.422998005419062E-5 1.1409993351396875E-5 0.0 7.416495678407969E-5 0.0 20-21 3.422998005419062E-5 1.1409993351396875E-5 0.0 1.0839493683827031E-4 0.0 22-23 3.422998005419062E-5 2.281998670279375E-5 0.0 2.1678987367654062E-4 0.0 24-25 3.422998005419062E-5 2.281998670279375E-5 0.0 3.5941479056900155E-4 0.0 26-27 3.422998005419062E-5 2.281998670279375E-5 0.0 6.78894604408114E-4 0.0 28-29 3.422998005419062E-5 2.281998670279375E-5 0.0 0.0022934086636307717 0.0 30-31 3.422998005419062E-5 2.281998670279375E-5 0.0 0.007479250641840651 0.0 32-33 3.422998005419062E-5 2.281998670279375E-5 0.0 0.019653713547781115 0.0 34-35 3.422998005419062E-5 2.281998670279375E-5 0.0 0.039313132092237926 0.0 36-37 3.422998005419062E-5 2.281998670279375E-5 0.0 0.07098156863903995 0.0 38-39 3.422998005419062E-5 2.281998670279375E-5 5.7049966756984374E-6 0.11465331819151149 0.0 40-41 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.16039027654058585 0.0 42-43 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.20855756347350776 0.0 44-45 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.27138098686629897 0.0 46-47 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.3482957520480653 0.0 48-49 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.4303564242313116 0.0 50-51 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.5180251081467695 0.0 52-53 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.6050548324345492 0.0 54-55 3.422998005419062E-5 2.281998670279375E-5 1.1409993351396875E-5 0.7079672674674733 0.0 56-57 3.993497672988906E-5 2.281998670279375E-5 1.1409993351396875E-5 0.8316458903999397 0.0 58-59 4.56399734055875E-5 2.281998670279375E-5 2.281998670279375E-5 0.970779349326873 0.0 60-61 4.56399734055875E-5 2.281998670279375E-5 2.281998670279375E-5 1.1173350089288903 0.0 62-63 5.134497008128593E-5 2.281998670279375E-5 2.281998670279375E-5 1.267644556343517 0.0 64-65 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 1.4371057775984633 0.0 66-67 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 1.6350520472551722 0.0 68-69 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 1.8589731667763356 0.0 70-71 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 2.1025137698652263 0.0 72-73 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 2.3598775798993343 0.0 74-75 5.704996675698437E-5 2.281998670279375E-5 2.281998670279375E-5 2.626072724787423 0.0 76-77 5.704996675698437E-5 2.281998670279375E-5 2.8524983378492186E-5 2.9189159091476995 0.0 78-79 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 3.251848105148109 0.0 80-81 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 3.6202425604846598 0.0 82-83 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 3.999961434222472 0.0 84-85 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 4.384170140344059 0.0 86-87 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 4.802956831317054 0.0 88 5.704996675698437E-5 2.281998670279375E-5 3.422998005419062E-5 5.144001532590307 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16105 0.0 57.449947 1 GTATCAA 27940 0.0 39.38403 1 GTGGTAT 6195 0.0 34.430607 1 ATCAACG 35020 0.0 31.066616 3 TCAACGC 35305 0.0 30.882473 4 TATCAAC 36215 0.0 30.132456 2 CAACGCA 36630 0.0 29.856665 5 TGGTATC 7125 0.0 29.191721 2 AACGCAG 38550 0.0 28.442463 6 ACGCAGA 43515 0.0 25.121185 7 CGCAGAG 43495 0.0 24.981464 8 GCAGAGT 46835 0.0 22.658066 9 GTACATG 22625 0.0 21.851866 1 GAGTACT 33660 0.0 21.145823 12-13 CAGAGTA 45555 0.0 20.792952 10-11 TACATGG 22850 0.0 20.655563 2 AGAGTAC 43290 0.0 20.111296 10-11 ACATGGG 23185 0.0 19.626446 3 GTACTTT 36485 0.0 19.328184 14-15 AGTACTT 35305 0.0 18.955847 12-13 >>END_MODULE