FastQCFastQC Report
Wed 25 May 2016
SRR2049534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066733
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72050.046.0952221
GTATCAA114950.034.8222581
TCAACGC118000.032.221024
ATCAACG118850.032.033693
CAACGCA133200.028.5441445
TATCAAC136800.028.0928632
AACGCAG143000.026.5875476
GTACATG196700.024.7533571
TACATGG196450.023.7361452
ACGCAGA166500.022.4035637
CGCAGAG166600.022.3889398
ACATGGG205700.021.9710923
GTGGTAT40250.019.2276381
CATGGGG140450.019.15384
GCAGAGT189000.018.300139
GAGTACA149050.018.2590181
CAGAGTA183300.016.61241510-11
TGGTATC43400.016.2933442
AGAGTAC162800.015.92709910-11
AGTACAT159050.015.7864062
Produced by FastQC (version 0.11.5)