FastQCFastQC Report
Wed 25 May 2016
SRR2049534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066733
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246620.3489872901664744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233040.32977048941851916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144230.20409714078627278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70940.10038585015168962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138400.058.1377451
GTATCAA259450.041.0660671
GTGGTAT56400.033.3912851
ATCAACG328350.032.286043
TCAACGC331700.031.9599694
TATCAAC335900.031.5741372
CAACGCA345100.030.7791045
TGGTATC59900.030.7140792
AACGCAG359400.029.5801856
ACGCAGA404050.026.1361777
CGCAGAG402650.026.0636448
GCAGAGT433100.023.7862769
GAGTACT318800.021.86957212-13
GTACATG201450.021.82891
CAGAGTA424500.020.99646410-11
TACATGG204750.020.9813982
AGAGTAC411150.020.9180810-11
AGTACTT330000.020.25147412-13
GTACTTT342500.020.20532214-15
ACATGGG213250.019.527423
Produced by FastQC (version 0.11.5)