##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7066733 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.649894371274534 31.0 31.0 34.0 30.0 34.0 2 31.93686262095936 33.0 31.0 34.0 30.0 34.0 3 31.916231588203487 33.0 31.0 34.0 30.0 34.0 4 35.585492900326074 37.0 35.0 37.0 33.0 37.0 5 35.72134322890082 37.0 35.0 37.0 33.0 37.0 6 35.806548938526475 37.0 35.0 37.0 35.0 37.0 7 35.79024791229554 37.0 35.0 37.0 33.0 37.0 8 35.77805755502578 37.0 35.0 37.0 33.0 37.0 9 37.501784063442045 39.0 37.0 39.0 35.0 39.0 10-11 37.461863777788125 39.0 37.0 39.0 34.5 39.0 12-13 37.446927738744336 39.0 37.0 39.0 34.0 39.0 14-15 38.70846542525379 40.0 38.0 41.0 34.0 41.0 16-17 38.71843919955657 40.0 38.0 41.0 34.5 41.0 18-19 38.79765168713746 40.0 38.0 41.0 35.0 41.0 20-21 38.82862165586276 40.0 38.0 41.0 35.0 41.0 22-23 38.80305029212226 40.0 38.0 41.0 35.0 41.0 24-25 38.766817353931444 40.0 38.0 41.0 34.0 41.0 26-27 38.67104466802411 40.0 38.0 41.0 34.0 41.0 28-29 38.58642734910177 40.0 38.0 41.0 34.0 41.0 30-31 38.46339333890215 40.0 38.0 41.0 34.0 41.0 32-33 38.3113602848728 40.0 38.0 41.0 34.0 41.0 34-35 38.20281981787058 40.0 38.0 41.0 34.0 41.0 36-37 38.05954901649744 40.0 38.0 41.0 33.5 41.0 38-39 37.96644899418161 40.0 37.5 41.0 33.0 41.0 40-41 37.83823076377726 40.0 37.0 41.0 33.0 41.0 42-43 37.69284752940291 40.0 37.0 41.0 33.0 41.0 44-45 37.49626170961886 40.0 37.0 41.0 33.0 41.0 46-47 37.23241687495481 40.0 37.0 41.0 32.0 41.0 48-49 36.983245298782336 40.0 37.0 41.0 31.0 41.0 50-51 36.76858740807103 40.0 36.5 41.0 31.0 41.0 52-53 36.667333405691146 40.0 36.0 41.0 31.0 41.0 54-55 36.544788942783036 40.0 36.0 41.0 31.0 41.0 56-57 36.37651889494056 39.0 35.5 41.0 30.0 41.0 58-59 36.21030036085982 39.0 35.0 41.0 30.0 41.0 60-61 36.0330144778358 39.0 35.0 41.0 30.0 41.0 62-63 35.8428597061754 39.0 35.0 41.0 29.5 41.0 64-65 35.58562372456976 38.0 35.0 40.0 29.0 41.0 66-67 35.3362247448715 38.0 35.0 40.0 29.0 41.0 68-69 35.03399130828913 37.0 34.5 40.0 28.5 41.0 70-71 34.751265259349694 37.0 34.0 39.5 28.0 41.0 72-73 34.44387668247831 36.0 34.0 39.0 28.0 41.0 74-75 34.10136954374815 36.0 34.0 39.0 28.0 41.0 76-77 33.807383270317416 35.0 34.0 38.5 28.0 40.0 78-79 33.47690127814366 35.0 34.0 37.0 27.5 39.0 80-81 33.16279877844543 35.0 34.0 37.0 27.0 39.0 82-83 32.867821594504846 35.0 33.5 36.5 27.0 39.0 84-85 32.58682682648403 35.0 33.0 36.0 26.0 37.5 86-87 32.31818500854638 35.0 33.0 36.0 26.0 37.0 88-89 32.09268639412299 35.0 33.0 35.0 26.0 37.0 90-91 31.933507393020225 35.0 33.0 35.0 26.0 36.0 92-93 31.772286712969063 35.0 33.0 35.0 26.0 36.0 94-95 31.58519842195821 35.0 33.0 35.0 25.0 36.0 96-97 31.413208196772118 35.0 33.0 35.0 25.0 36.0 98-99 31.234309828884154 35.0 33.0 35.0 24.5 35.0 100 31.12052443469988 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44154003321701296 1101 2 0.46017701760321117 1101 3 0.54326517291436 1101 4 0.42540341069777554 1101 5 0.3373452567713713 1101 6 0.3378239655822384 1101 7 0.3026765640593254 1101 8 0.3025305814527641 1101 9 0.35338482986340125 1101 10-11 0.34067928923827395 1101 12-13 0.3189933147951649 1101 14-15 0.49296287022776397 1101 16-17 0.4970753817537954 1101 18-19 0.46866373128158045 1101 20-21 0.46181746784744604 1101 22-23 0.5026682556633943 1101 24-25 0.5156720302719862 1101 26-27 0.49945661851221956 1101 28-29 0.540109253320459 1101 30-31 0.530321044634114 1101 32-33 0.497677012266017 1101 34-35 0.5798951888337953 1101 36-37 0.5971056783492159 1101 38-39 0.6107727434457502 1101 40-41 0.5934593700329742 1101 42-43 0.5662914313003284 1101 44-45 0.5843141325907766 1101 46-47 0.595697844108507 1101 48-49 0.6207853362529221 1101 50-51 0.6600178087778801 1101 52-53 0.6298431888366665 1101 54-55 0.5657660501034414 1101 56-57 0.5292916963758358 1101 58-59 0.5861002400996469 1101 60-61 0.6527024562618919 1101 62-63 0.6347478141291774 1101 64-65 0.6020451086724918 1101 66-67 0.6424922727722375 1101 68-69 0.609517488823414 1101 70-71 0.6118341379671435 1101 72-73 0.6024696045878315 1101 74-75 0.5934159086019619 1101 76-77 0.5366991053097436 1101 78-79 0.5527401368144069 1101 80-81 0.5317635603319175 1101 82-83 0.5611444212452739 1101 84-85 0.570326512710011 1101 86-87 0.6247732568414079 1101 88-89 0.6018001036001905 1101 90-91 0.6416040187699217 1101 92-93 0.6137931951339191 1101 94-95 0.5776496230778143 1101 96-97 0.6122572776176263 1101 98-99 0.6540762311283004 1101 100 0.6961412902655262 1102 1 0.3941759565997849 1102 2 0.4454739477056826 1102 3 0.5010244955115368 1102 4 0.43278146717263866 1102 5 0.35606768547386025 1102 6 0.3178281199350508 1102 7 0.34482111262795456 1102 8 0.35604280450729675 1102 9 0.4411319446975668 1102 10-11 0.3918005648963572 1102 12-13 0.3940397209150177 1102 14-15 0.4839500714296463 1102 16-17 0.5200541410622108 1102 18-19 0.49409152986127225 1102 20-21 0.4683708255830723 1102 22-23 0.4767283689015187 1102 24-25 0.46833028732520177 1102 26-27 0.3763343453677592 1102 28-29 0.42077003630104315 1102 30-31 0.522537379131613 1102 32-33 0.5268572137390279 1102 34-35 0.5208452870383056 1102 36-37 0.5427081566463912 1102 38-39 0.5141050889754553 1102 40-41 0.5100487399582505 1102 42-43 0.5359699947088075 1102 44-45 0.4980219302825546 1102 46-47 0.4798714799596837 1102 48-49 0.5430451329247106 1102 50-51 0.5928622398320016 1102 52-53 0.536892160338617 1102 54-55 0.47043887909755 1102 56-57 0.509816197642941 1102 58-59 0.4542456655818441 1102 60-61 0.5061543011999419 1102 62-63 0.48296389430451825 1102 64-65 0.532497417858913 1102 66-67 0.5527235139021656 1102 68-69 0.4962513269479274 1102 70-71 0.5063076386951693 1102 72-73 0.5395804919681453 1102 74-75 0.5473489723128822 1102 76-77 0.4913801902284547 1102 78-79 0.45828308991390543 1102 80-81 0.45297243919232955 1102 82-83 0.4326206284791212 1102 84-85 0.43903269440421866 1102 86-87 0.4582972584856293 1102 88-89 0.45277823133317696 1102 90-91 0.4896565479745867 1102 92-93 0.5130266734303852 1102 94-95 0.5402786590991404 1102 96-97 0.609094478009073 1102 98-99 0.6497287222482697 1102 100 0.6729246513974836 1103 1 0.4235394835010773 1103 2 0.4310067913204101 1103 3 0.5010112723244688 1103 4 0.42778713272426927 1103 5 0.2980944514157784 1103 6 0.30728271295513565 1103 7 0.3513028906660125 1103 8 0.3484044122185779 1103 9 0.3630463411905964 1103 10-11 0.3550783655994749 1103 12-13 0.3285995100502106 1103 14-15 0.4486384152660392 1103 16-17 0.4029757083923968 1103 18-19 0.43690991264141843 1103 20-21 0.4432549822611733 1103 22-23 0.4412662733500383 1103 24-25 0.44350386506131656 1103 26-27 0.4421001248296079 1103 28-29 0.4496742380110845 1103 30-31 0.4538763904402501 1103 32-33 0.4193186685499697 1103 34-35 0.4244024714820611 1103 36-37 0.506840364264157 1103 38-39 0.4982841475130755 1103 40-41 0.5088794820938034 1103 42-43 0.5244772128034754 1103 44-45 0.5655399737359232 1103 46-47 0.5295531114459493 1103 48-49 0.473718357210295 1103 50-51 0.4883304093113523 1103 52-53 0.4552984087447953 1103 54-55 0.4308363165633793 1103 56-57 0.5156718314696107 1103 58-59 0.438974119455132 1103 60-61 0.43233898017163597 1103 62-63 0.4223533878716097 1103 64-65 0.5010771771273639 1103 66-67 0.48401292265540263 1103 68-69 0.47655634387680834 1103 70-71 0.4912909888925938 1103 72-73 0.5443449409080969 1103 74-75 0.472028441545028 1103 76-77 0.49501809139569986 1103 78-79 0.45752197646270787 1103 80-81 0.4341009227397592 1103 82-83 0.4682257850049254 1103 84-85 0.4392592406789504 1103 86-87 0.47732896453177887 1103 88-89 0.48902562637423586 1103 90-91 0.441419106079028 1103 92-93 0.41215971206706215 1103 94-95 0.4412746914296939 1103 96-97 0.4493457702791339 1103 98-99 0.4831037343301432 1103 100 0.4901865903393521 1104 1 0.4199593969686113 1104 2 0.39496884862873927 1104 3 0.46715021997803063 1104 4 0.3946330497958357 1104 5 0.28613205653044815 1104 6 0.2948808320118701 1104 7 0.2632724874230732 1104 8 0.31483428798887303 1104 9 0.3478984157765481 1104 10-11 0.31904685130882626 1104 12-13 0.3409100441025146 1104 14-15 0.43779883343524517 1104 16-17 0.4453432627055136 1104 18-19 0.3989001352841086 1104 20-21 0.4300234938216363 1104 22-23 0.41004587579296015 1104 24-25 0.4238869290091287 1104 26-27 0.4996134216505368 1104 28-29 0.4641075280529705 1104 30-31 0.5160942627013938 1104 32-33 0.5031270217586368 1104 34-35 0.5131333212066664 1104 36-37 0.5249465229473458 1104 38-39 0.5117462849186012 1104 40-41 0.5116558294282214 1104 42-43 0.5225165934631377 1104 44-45 0.5460925738694442 1104 46-47 0.591810688536917 1104 48-49 0.6165822562727143 1104 50-51 0.646859685693407 1104 52-53 0.6406580863759572 1104 54-55 0.5872094745858476 1104 56-57 0.6040660925231975 1104 58-59 0.6257229247007032 1104 60-61 0.5959902139777356 1104 62-63 0.6364722878518805 1104 64-65 0.6974075656571657 1104 66-67 0.6828615943139908 1104 68-69 0.5873531613714107 1104 70-71 0.6091974116558774 1104 72-73 0.6541571559700827 1104 74-75 0.6830054863093906 1104 76-77 0.633133937369827 1104 78-79 0.6034733213735777 1104 80-81 0.626803183611834 1104 82-83 0.6269878846650769 1104 84-85 0.6529302237705394 1104 86-87 0.6909833008148425 1104 88-89 0.6467679134961983 1104 90-91 0.6112033954107083 1104 92-93 0.6395532429642934 1104 94-95 0.6871830915278743 1104 96-97 0.7077722352154758 1104 98-99 0.7528492555909629 1104 100 0.8145925132686678 1105 1 0.32832298547276295 1105 2 0.32323377287034205 1105 3 0.4046445492627839 1105 4 0.3162648459834969 1105 5 0.23727764910460536 1105 6 0.25769973230202226 1105 7 0.2546928224813598 1105 8 0.2837262730039356 1105 9 0.3220511421504497 1105 10-11 0.26077637315209756 1105 12-13 0.25588385067656816 1105 14-15 0.3351434935229989 1105 16-17 0.3305178423356878 1105 18-19 0.3194002993931093 1105 20-21 0.31657271875925375 1105 22-23 0.32316527751524404 1105 24-25 0.3160826301220112 1105 26-27 0.30957988214052534 1105 28-29 0.3298286333224567 1105 30-31 0.3481845671204482 1105 32-33 0.3168161043971125 1105 34-35 0.222661717734411 1105 36-37 0.28723243194359327 1105 38-39 0.30726534440179876 1105 40-41 0.32448344703668397 1105 42-43 0.2989539032759865 1105 44-45 0.34100757021587924 1105 46-47 0.38294559219662716 1105 48-49 0.4283531881960201 1105 50-51 0.543579698568422 1105 52-53 0.47473625986803114 1105 54-55 0.4256394136339381 1105 56-57 0.4552018027220228 1105 58-59 0.5059079058085274 1105 60-61 0.47317522794268996 1105 62-63 0.4328445013446327 1105 64-65 0.4890294757682341 1105 66-67 0.4912876559513819 1105 68-69 0.4554512142860716 1105 70-71 0.502120831776729 1105 72-73 0.4479497018965901 1105 74-75 0.4409393689787464 1105 76-77 0.4339923017499032 1105 78-79 0.4335329019688672 1105 80-81 0.3978717466537276 1105 82-83 0.412842611123061 1105 84-85 0.39437572321920555 1105 86-87 0.4642671377592791 1105 88-89 0.4152476104891747 1105 90-91 0.393272342694285 1105 92-93 0.4202529748632209 1105 94-95 0.43118586589455177 1105 96-97 0.43291417491107964 1105 98-99 0.5136661791032111 1105 100 0.5515695521903723 1106 1 0.2688577080122272 1106 2 0.27976562807733174 1106 3 0.38085874374953477 1106 4 0.3216709996522482 1106 5 0.19485928453458 1106 6 0.24589260514279943 1106 7 0.19694629853582057 1106 8 0.23509042875314634 1106 9 0.2401813810130662 1106 10-11 0.2528051448449773 1106 12-13 0.22010833410790553 1106 14-15 0.32088791501587366 1106 16-17 0.32878687810626417 1106 18-19 0.29594624672321146 1106 20-21 0.30358003115736665 1106 22-23 0.3108687549959086 1106 24-25 0.3372665663690526 1106 26-27 0.3270356102374379 1106 28-29 0.3196901128819789 1106 30-31 0.26441777035049796 1106 32-33 0.28346902514110184 1106 34-35 0.28358180473416894 1106 36-37 0.30492467256102174 1106 38-39 0.28750226654588573 1106 40-41 0.2952528733998179 1106 42-43 0.2576550248037037 1106 44-45 0.2872868245752187 1106 46-47 0.23016943508946497 1106 48-49 0.23769614118921112 1106 50-51 0.26554860420755944 1106 52-53 0.26572310096685214 1106 54-55 0.19862125165452227 1106 56-57 0.20423151603727518 1106 58-59 0.1448653972569005 1106 60-61 0.18402193976018566 1106 62-63 0.1937340152502287 1106 64-65 0.17033390763769063 1106 66-67 0.19749395913191847 1106 68-69 0.2025419993879609 1106 70-71 0.20909569909610326 1106 72-73 0.18347209027398748 1106 74-75 0.20015339900675855 1106 76-77 0.2122009998553125 1106 78-79 0.18901155827780514 1106 80-81 0.16158834966936553 1106 82-83 0.1921951883202695 1106 84-85 0.23064236723335796 1106 86-87 0.28110352509682457 1106 88-89 0.23957068906197065 1106 90-91 0.2614265687457724 1106 92-93 0.261269761459598 1106 94-95 0.24971007374964316 1106 96-97 0.28276857371858455 1106 98-99 0.27115421286710983 1106 100 0.3057752719863558 1107 1 0.21171135246396133 1107 2 0.26355256437683394 1107 3 0.3287803200231707 1107 4 0.2440051050324783 1107 5 0.1872914787500548 1107 6 0.18972851375976774 1107 7 0.1715790049728696 1107 8 0.1882607545854924 1107 9 0.16356338928026304 1107 10-11 0.21698826527955362 1107 12-13 0.18028697638992952 1107 14-15 0.279347977178972 1107 16-17 0.21290749077825666 1107 18-19 0.2029111636065366 1107 20-21 0.27072922989453474 1107 22-23 0.2751904613870835 1107 24-25 0.2765938276241968 1107 26-27 0.2931507272542646 1107 28-29 0.2992628856502222 1107 30-31 0.3504184509444528 1107 32-33 0.28798689580710146 1107 34-35 0.2690379581547262 1107 36-37 0.3877120776927825 1107 38-39 0.31762592469696926 1107 40-41 0.33038253603618273 1107 42-43 0.32933341905331304 1107 44-45 0.344860797090071 1107 46-47 0.3771968346776262 1107 48-49 0.3326791656604442 1107 50-51 0.40511901555777996 1107 52-53 0.36603775077781364 1107 54-55 0.3454542408534209 1107 56-57 0.31947428656246046 1107 58-59 0.33112246837140447 1107 60-61 0.33288376826523347 1107 62-63 0.34538331460454685 1107 64-65 0.3753362984473583 1107 66-67 0.3572387349791484 1107 68-69 0.3765295386528038 1107 70-71 0.33738989811923403 1107 72-73 0.3618121071736269 1107 74-75 0.3500933060872242 1107 76-77 0.2772182418788631 1107 78-79 0.27618369305974255 1107 80-81 0.32467175675368054 1107 82-83 0.3296208997466934 1107 84-85 0.3377034799091376 1107 86-87 0.3592981423780799 1107 88-89 0.3127918808849657 1107 90-91 0.2534925020458978 1107 92-93 0.26022798117609014 1107 94-95 0.2676108445184404 1107 96-97 0.27747198590301636 1107 98-99 0.3370830924216932 1107 100 0.30585776559713906 1108 1 0.18341170927059025 1108 2 0.21098215833427147 1108 3 0.2884858917960855 1108 4 0.24570829933413307 1108 5 0.1876095347944826 1108 6 0.19062717781281435 1108 7 0.14468616243320298 1108 8 0.21132190966910258 1108 9 0.20474379092898687 1108 10-11 0.18557906874620755 1108 12-13 0.1597508850709346 1108 14-15 0.24742160255116374 1108 16-17 0.24543527582331848 1108 18-19 0.25529948465553076 1108 20-21 0.20809585042866985 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2215 52-53 -0.8586195855359122 2215 54-55 -0.8234165776232345 2215 56-57 -0.7584297248657705 2215 58-59 -0.7302598615927707 2215 60-61 -0.9466051041888477 2215 62-63 -0.9306676066454997 2215 64-65 -0.8134564641290538 2215 66-67 -0.7185284822701021 2215 68-69 -0.7394710800924713 2215 70-71 -0.7468241587749347 2215 72-73 -0.6642069147830014 2215 74-75 -0.7766008183722093 2215 76-77 -0.7015265323485878 2215 78-79 -0.6933943277007657 2215 80-81 -0.7489442824939019 2215 82-83 -0.7438239945464247 2215 84-85 -0.7466207907921998 2215 86-87 -0.7366451487132792 2215 88-89 -0.6936345795175285 2215 90-91 -0.7695620148334363 2215 92-93 -0.8779697511750193 2215 94-95 -0.9317843339993317 2215 96-97 -1.0767365170172205 2215 98-99 -1.041970359728385 2215 100 -1.07979912780986 2216 1 -1.0821693682902378 2216 2 -1.5542974168946202 2216 3 -1.2284115465763037 2216 4 -1.5582201250984937 2216 5 -0.9077917193467897 2216 6 -1.1225054501745788 2216 7 -0.8675232824831198 2216 8 -0.8214835750785667 2216 9 -0.9553624427041569 2216 10-11 -1.1489658769338789 2216 12-13 -0.9496865455002421 2216 14-15 -1.2501445658131445 2216 16-17 -1.2380278874646322 2216 18-19 -1.328312025558155 2216 20-21 -1.1644372350793546 2216 22-23 -1.1114486174105025 2216 24-25 -1.15866757771785 2216 26-27 -1.3085820267306687 2216 28-29 -1.5436222026372164 2216 30-31 -1.6465245599869363 2216 32-33 -1.4600398918432589 2216 34-35 -1.4411138740029372 2216 36-37 -1.4305883349864814 2216 38-39 -1.4453343236791198 2216 40-41 -1.3764192066667604 2216 42-43 -1.8290622699020247 2216 44-45 -1.6312753478123199 2216 46-47 -1.5527404942192788 2216 48-49 -1.50878327263991 2216 50-51 -1.6723224444441058 2216 52-53 -1.6783044042436117 2216 54-55 -1.766162243597016 2216 56-57 -1.7475847963453575 2216 58-59 -1.6496912338377712 2216 60-61 -1.8244126067494832 2216 62-63 -1.783437798380767 2216 64-65 -1.8327646303183158 2216 66-67 -1.7221509515068547 2216 68-69 -2.0618584303411183 2216 70-71 -1.794803369247866 2216 72-73 -1.6763267985287413 2216 74-75 -1.7687702536600867 2216 76-77 -1.9532066400012482 2216 78-79 -1.8906258775609253 2216 80-81 -1.6774406047625092 2216 82-83 -1.8137062548149743 2216 84-85 -1.8757669231983307 2216 86-87 -2.0470522432953295 2216 88-89 -1.8563938189029159 2216 90-91 -1.7605801058135562 2216 92-93 -1.8313599390618442 2216 94-95 -1.8609887162710983 2216 96-97 -2.045491117887778 2216 98-99 -2.0968194943199627 2216 100 -2.1184692447530686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 399.0 3 50.0 4 65.0 5 137.0 6 435.0 7 1269.0 8 2845.0 9 4739.0 10 6332.0 11 8798.0 12 12356.0 13 17583.0 14 21809.0 15 27913.0 16 36110.0 17 51317.0 18 51481.0 19 27051.0 20 19244.0 21 18127.0 22 19054.0 23 20697.0 24 24574.0 25 29947.0 26 38417.0 27 49826.0 28 65212.0 29 85637.0 30 111355.0 31 143003.0 32 185350.0 33 240654.0 34 325573.0 35 471900.0 36 762961.0 37 1406304.0 38 1978099.0 39 797387.0 40 2723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.056216417661076 21.03828223805422 15.350219157770372 22.555282186514336 2 15.426193766930574 20.91174598933849 40.01716777795435 23.64489246577659 3 18.167107771016678 25.479015550750255 31.00503443387489 25.348842244358178 4 12.592930515778086 17.667420146166997 38.33122826603611 31.408421072018804 5 12.427840141556013 37.855308675937884 35.708222990342456 14.008628192163645 6 30.010068469840228 38.73945304788785 17.21152206537359 14.038956416898332 7 25.93055376508494 33.40954016516543 22.42909134956705 18.23081472018258 8 24.650400687276566 36.4915159522795 20.19525854450706 18.66282481593687 9 24.438513366364166 16.837488224753425 22.111298703742932 36.61269970513948 10-11 23.550536011478005 27.43907969920471 28.542666038182002 20.467718251135285 12-13 24.440979224909054 24.908960747344032 29.12634864319179 21.523711384555124 14-15 21.46321414124962 26.87031860065981 27.207589138994333 24.458878119096237 16-17 20.085702969108922 30.257914088447947 28.006322016128244 21.65006092631489 18-19 19.929980374240827 28.274260538780794 31.417820653475943 20.37793843350244 20-21 21.072106445793267 27.41568133393465 31.299845345791326 20.212366874480754 22-23 20.758963158789218 27.257227632627412 31.459990068961147 20.523819139622226 24-25 20.617072981248903 27.432202405269873 31.358040554241967 20.592684059239254 26-27 20.384002055829757 27.46002997424694 31.396311421416375 20.75965654850693 28-29 20.364222619766277 27.424561203907555 31.61380906168376 20.597407114642404 30-31 20.736696269544893 27.818858885069808 31.11793936967711 20.326505475708185 32-33 20.380264934380552 27.539770510579785 31.512441891448816 20.567522663590847 34-35 20.589712389020498 27.335644349376153 31.388804416411375 20.685838845191974 36-37 20.833693589385646 27.632004775049516 30.90938910526264 20.624912530302193 38-39 20.734998761096534 27.711949779339335 30.98798978254874 20.56506167701539 40-41 20.64214114216569 27.520659122114843 31.05225568873198 20.784944046987484 42-43 20.692008598598534 27.542748537407597 30.85549574322392 20.90974712076995 44-45 20.901843893069117 27.66978036385413 30.571863971654228 20.85651177142252 46-47 21.017583372684378 27.884066088247568 30.12692003504307 20.971430504024983 48-49 21.07454746061582 27.918091712252323 29.802385345533782 21.204975481598073 50-51 21.175166799141838 28.015145046515837 29.462688062503567 21.347000091838762 52-53 21.149603359855256 28.109467274340204 29.41404465118464 21.326884714619894 54-55 21.26758574294515 28.333856677477414 29.27423464279746 21.12432293677998 56-57 21.1207852341386 28.10503099522792 29.31277437537261 21.461409395260866 58-59 21.012792499602714 27.800341289925353 29.707237545457797 21.47962866501413 60-61 21.107073098700628 28.073623271177784 29.4063890626687 21.41291456745288 62-63 20.9369662048927 28.041324046062023 29.514232389988415 21.507477359056864 64-65 20.979149771188467 27.876509555405587 29.615672475527234 21.52866819787871 66-67 20.911112673989525 27.91358467908721 29.466862551620387 21.708440095302876 68-69 21.27026325605669 28.07533949214432 29.25517056540421 21.399226686394787 70-71 21.20162881489933 27.923440718646088 29.212989934669952 21.661940531784634 72-73 21.073868221708675 28.03835237584326 29.328227060510137 21.559552341937923 74-75 21.22429841342527 28.059408781964734 29.099500433934605 21.616792370675388 76-77 21.259066954984714 28.05456212934605 28.93665290594678 21.749718009722454 78-79 21.32205512454496 28.10515479235431 29.075788289483462 21.497001793617272 80-81 21.17288925690542 28.019135916331095 29.120496853451566 21.687477973311914 82-83 21.082916637893266 28.014824999235856 29.286922970682827 21.61533539218805 84-85 21.100408066924278 27.950405088178655 29.090670328141734 21.858516516755337 86-87 21.210204206102027 28.077040691929355 28.977400164970152 21.73535493699847 88-89 21.08818292149596 27.92238074055657 29.056420827439812 21.933015510507662 90-91 20.9268151347282 27.885361364224103 29.048318334052077 22.139505166995615 92-93 21.009323052404973 28.05492595741959 28.98716751876448 21.948583471410963 94-95 20.810981386604894 27.864111673295188 29.116558895719848 22.208348044380074 96-97 20.791580777142705 27.962454503375177 29.038298178238797 22.207666541243317 98-99 20.824952808805598 27.92960224963974 28.988826022327295 22.25661891922737 100 20.924335226684978 27.840009781145454 28.91928955940553 22.316365432764034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1446.0 1 1053.0 2 1119.5 3 1638.0 4 1141.5 5 771.5 6 2046.5 7 5567.0 8 9220.0 9 10339.0 10 7992.0 11 5096.0 12 4530.5 13 4909.0 14 5558.5 15 6337.0 16 7122.5 17 8115.0 18 9268.5 19 10627.5 20 12626.5 21 15388.5 22 19800.5 23 26703.0 24 35251.0 25 45234.5 26 59561.5 27 77378.5 28 98707.0 29 120558.0 30 138553.5 31 157081.5 32 175439.0 33 192181.0 34 208159.5 35 220803.5 36 231375.5 37 241385.0 38 249219.0 39 253607.0 40 255723.0 41 253524.0 42 248724.5 43 244774.0 44 239240.5 45 233471.5 46 227514.0 47 221232.0 48 215291.5 49 212297.0 50 208408.0 51 198496.0 52 186157.0 53 175972.5 54 168353.5 55 158852.0 56 144881.5 57 129247.0 58 115817.5 59 104042.5 60 89896.0 61 74665.0 62 61572.0 63 49891.5 64 40076.0 65 31837.5 66 24728.0 67 18870.0 68 14251.0 69 10655.0 70 7785.0 71 5748.5 72 4268.5 73 2957.5 74 2005.5 75 1306.0 76 796.0 77 472.5 78 297.0 79 191.5 80 112.5 81 56.0 82 29.5 83 16.5 84 9.5 85 5.0 86 5.5 87 5.5 88 2.0 89 1.0 90 0.5 91 0.5 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 1.0 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060013587608305 2 8.490486339302758E-5 3 0.0 4 0.11841398281214248 5 0.0018537561840811024 6 0.0016697956467295423 7 0.0 8 0.0 9 2.8301621131009194E-5 10-11 0.0 12-13 1.4150810565504597E-5 14-15 3.5377026413761495E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.5565891622055058E-4 30-31 3.042424271583488E-4 32-33 2.8301621131009194E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.9811134791706435E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.4150810565504597E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.1320648452403678E-4 80-81 3.042424271583488E-4 82-83 2.9716702187559653E-4 84-85 0.0 86-87 0.0 88-89 1.0613107924128448E-4 90-91 7.782945811027529E-5 92-93 7.924453916682574E-4 94-95 6.792389071442206E-4 96-97 0.0 98-99 2.1226215848256894E-5 100 0.0010754616029783493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7066733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.7820753738277 #Duplication Level Percentage of deduplicated Percentage of total 1 71.77040997726749 40.03502418962451 2 16.026756777008934 17.880115090662326 3 5.541203808970366 9.272995456011783 4 2.425309753727863 5.411552459493087 5 1.2807769992326195 3.5722199554129426 6 0.7441421811065289 2.4905877141197363 7 0.48861246709342876 1.9079072227598286 8 0.33397672040969123 1.490393167279774 9 0.2315773767031501 1.1626080013915567 >10 1.082180881404526 10.286814961595224 >50 0.04946286977722835 1.874393193413721 >100 0.023230393489688524 2.4426824432469423 >500 0.0015986263692181057 0.6098003072192538 >1k 6.596784501609687E-4 0.5795013982257032 >5k 2.5372247261801847E-5 0.10040256025389722 >10k+ 7.611674178540554E-5 0.8830018792895961 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24662 0.3489872901664744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23304 0.32977048941851916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14423 0.20409714078627278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7094 0.10038585015168962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8301621131009194E-5 0.0 5 0.0 0.0 0.0 2.8301621131009194E-5 0.0 6 0.0 0.0 0.0 4.2452431696513795E-5 0.0 7 0.0 0.0 0.0 4.2452431696513795E-5 0.0 8 0.0 0.0 0.0 4.2452431696513795E-5 0.0 9 0.0 0.0 0.0 5.660324226201839E-5 0.0 10-11 0.0 0.0 0.0 8.490486339302759E-5 0.0 12-13 0.0 0.0 0.0 1.1320648452403678E-4 0.0 14-15 0.0 0.0 0.0 1.6980972678605515E-4 0.0 16-17 0.0 0.0 0.0 2.3348837433082584E-4 0.0 18-19 0.0 0.0 0.0 2.4056377961357816E-4 0.0 20-21 0.0 0.0 0.0 3.0424242715834887E-4 0.0 22-23 0.0 0.0 0.0 4.6697674866165174E-4 0.0 24-25 0.0 0.0 0.0 7.712191758200006E-4 0.0 26-27 0.0 0.0 0.0 0.0011957434927851384 0.0 28-29 0.0 0.0 0.0 0.003007047245169727 0.0 30-31 0.0 0.0 0.0 0.008695673092502576 0.0 32-33 0.0 0.0 0.0 0.02182762529729084 0.0 34-35 0.0 7.075405282752299E-6 0.0 0.04193592711087288 0.0 36-37 0.0 1.4150810565504597E-5 0.0 0.07618796408467676 0.0 38-39 0.0 1.4150810565504597E-5 0.0 0.12218517382784944 0.0 40-41 0.0 1.4150810565504597E-5 0.0 0.17073660487809572 0.0 42-43 0.0 1.4150810565504597E-5 0.0 0.22146018535014694 0.0 44-45 0.0 2.1226215848256894E-5 0.0 0.2834265848164916 0.0 46-47 0.0 2.8301621131009194E-5 0.0 0.3572938159684256 0.0 48-49 0.0 2.8301621131009194E-5 0.0 0.43583789001225887 0.0 50-51 0.0 2.8301621131009194E-5 0.0 0.5159385532182976 0.0 52-53 0.0 2.8301621131009194E-5 0.0 0.5952821480590819 0.0 54-55 0.0 2.8301621131009194E-5 0.0 0.6855713948722839 0.0 56-57 0.0 2.8301621131009194E-5 0.0 0.793089253548988 0.0 58-59 0.0 2.8301621131009194E-5 0.0 0.9104206993528693 0.0 60-61 0.0 2.8301621131009194E-5 0.0 1.0306035334856998 0.0 62-63 0.0 2.8301621131009194E-5 0.0 1.1509915543717302 0.0 64-65 0.0 2.8301621131009194E-5 0.0 1.2811860869796554 0.0 66-67 0.0 2.8301621131009194E-5 0.0 1.4329040024577129 0.0 68-69 0.0 2.8301621131009194E-5 0.0 1.602713729243768 0.0 70-71 0.0 2.8301621131009194E-5 0.0 1.7831719409803655 0.0 72-73 0.0 2.8301621131009194E-5 0.0 1.9680522810186827 0.0 74-75 0.0 2.8301621131009194E-5 0.0 2.1553099572320056 0.0 76-77 7.075405282752299E-6 2.8301621131009194E-5 0.0 2.363496682271709 0.0 78-79 1.4150810565504597E-5 2.8301621131009194E-5 0.0 2.5939992355732135 0.0 80-81 1.4150810565504597E-5 2.8301621131009194E-5 0.0 2.842692655856674 0.0 82-83 1.4150810565504597E-5 4.2452431696513795E-5 0.0 3.1016029613684286 0.0 84-85 1.4150810565504597E-5 4.2452431696513795E-5 0.0 3.3637184254732704 0.0 86-87 1.4150810565504597E-5 5.660324226201839E-5 0.0 3.644873805193998 0.0 88 1.4150810565504597E-5 5.660324226201839E-5 0.0 3.8730343993469116 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13840 0.0 58.137745 1 GTATCAA 25945 0.0 41.066067 1 GTGGTAT 5640 0.0 33.391285 1 ATCAACG 32835 0.0 32.28604 3 TCAACGC 33170 0.0 31.959969 4 TATCAAC 33590 0.0 31.574137 2 CAACGCA 34510 0.0 30.779104 5 TGGTATC 5990 0.0 30.714079 2 AACGCAG 35940 0.0 29.580185 6 ACGCAGA 40405 0.0 26.136177 7 CGCAGAG 40265 0.0 26.063644 8 GCAGAGT 43310 0.0 23.786276 9 GAGTACT 31880 0.0 21.869572 12-13 GTACATG 20145 0.0 21.8289 1 CAGAGTA 42450 0.0 20.996464 10-11 TACATGG 20475 0.0 20.981398 2 AGAGTAC 41115 0.0 20.91808 10-11 AGTACTT 33000 0.0 20.251474 12-13 GTACTTT 34250 0.0 20.205322 14-15 ACATGGG 21325 0.0 19.52742 3 >>END_MODULE