FastQCFastQC Report
Wed 25 May 2016
SRR2049533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.044.3672181
GTATCAA99800.033.3914341
TCAACGC101250.031.6451444
ATCAACG103200.030.9976023
CAACGCA110450.029.0550775
AACGCAG119600.027.0887496
TATCAAC122050.026.671482
GTACATG160150.023.3974631
ACGCAGA139350.022.8450577
GTGGTAT37150.022.0468141
CGCAGAG142550.022.0239738
TACATGG163750.021.8173452
ACATGGG164450.020.3550263
GCAGAGT165950.017.9370949
TGGTATC43100.017.8131942
GTATAGG19750.017.6248931
CATGGGG118300.016.8735944
CAGAGTA158050.016.44367610-11
AGAGTAC140600.016.34488310-11
GTATAGA27300.014.8132091
Produced by FastQC (version 0.11.5)