FastQCFastQC Report
Wed 25 May 2016
SRR2049533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223050.34423001509797013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222220.34294908744707875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137120.21161542107255624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133600.059.3364641
GTATCAA231750.039.0408861
GTGGTAT51000.033.1367761
ATCAACG291500.030.74523
TCAACGC293650.030.5681574
TATCAAC300700.029.8832
CAACGCA304800.029.420395
TGGTATC57400.028.2768762
AACGCAG320900.028.0317696
CGCAGAG360150.024.7547688
ACGCAGA362600.024.6911987
GCAGAGT385700.022.7981249
GAGTACT289400.021.81795912-13
GTACATG168300.021.7331621
CAGAGTA378600.021.25191710-11
TACATGG171200.020.4452972
AGAGTAC361800.020.22530210-11
ACATGGG171800.019.7986723
GTACTTT321050.019.41822614-15
AGTACTT303500.019.0622612-13
Produced by FastQC (version 0.11.5)