##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049533_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6479679
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685700788573016	31.0	31.0	34.0	30.0	34.0
2	31.825419746873262	31.0	31.0	34.0	30.0	34.0
3	31.947030863720254	33.0	31.0	34.0	30.0	34.0
4	35.63447386822711	37.0	35.0	37.0	33.0	37.0
5	35.71078088899157	37.0	35.0	37.0	33.0	37.0
6	35.81234132122903	37.0	35.0	37.0	35.0	37.0
7	35.808065646461806	37.0	35.0	37.0	35.0	37.0
8	35.811788207409656	37.0	35.0	37.0	35.0	37.0
9	37.547765406280156	39.0	37.0	39.0	35.0	39.0
10-11	37.50136326197641	39.0	37.0	39.0	35.0	39.0
12-13	37.47897079469523	39.0	37.0	39.0	34.5	39.0
14-15	38.75159625962952	40.0	38.0	41.0	34.5	41.0
16-17	38.75629109096299	40.0	38.0	41.0	35.0	41.0
18-19	38.83370040398606	40.0	38.0	41.0	35.0	41.0
20-21	38.86264620515924	40.0	38.0	41.0	35.0	41.0
22-23	38.834966670416854	40.0	38.0	41.0	35.0	41.0
24-25	38.793465771992715	40.0	38.0	41.0	35.0	41.0
26-27	38.70185421222255	40.0	38.0	41.0	34.5	41.0
28-29	38.61049497976674	40.0	38.0	41.0	34.0	41.0
30-31	38.488430445397064	40.0	38.0	41.0	34.0	41.0
32-33	38.33614805609969	40.0	38.0	41.0	34.0	41.0
34-35	38.22652348982102	40.0	38.0	41.0	34.0	41.0
36-37	38.08168375316123	40.0	38.0	41.0	33.5	41.0
38-39	37.9870134770565	40.0	37.5	41.0	33.0	41.0
40-41	37.862366870334164	40.0	37.0	41.0	33.0	41.0
42-43	37.710204625877296	40.0	37.0	41.0	33.0	41.0
44-45	37.52145877905372	40.0	37.0	41.0	33.0	41.0
46-47	37.26466480824127	40.0	37.0	41.0	32.0	41.0
48-49	37.02687139285758	40.0	37.0	41.0	31.0	41.0
50-51	36.815412306689886	40.0	36.0	41.0	31.0	41.0
52-53	36.71175863804365	40.0	36.0	41.0	31.0	41.0
54-55	36.58234574583093	39.5	36.0	41.0	31.0	41.0
56-57	36.413428427550194	39.0	35.0	41.0	30.5	41.0
58-59	36.24423424370251	39.0	35.0	41.0	30.0	41.0
60-61	36.05839525383897	39.0	35.0	41.0	30.0	41.0
62-63	35.856161470344446	39.0	35.0	41.0	30.0	41.0
64-65	35.58122362234302	38.0	35.0	40.0	29.0	41.0
66-67	35.330605420422835	37.5	35.0	40.0	29.0	41.0
68-69	35.02669082835739	37.0	34.5	40.0	29.0	41.0
70-71	34.73789303451606	36.5	34.0	39.5	29.0	41.0
72-73	34.42189018931339	36.0	34.0	39.0	28.0	41.0
74-75	34.089936399627206	36.0	34.0	39.0	28.0	40.5
76-77	33.79866440914743	35.0	34.0	38.5	28.0	40.0
78-79	33.47616587179704	35.0	34.0	37.0	28.0	39.0
80-81	33.172282299786765	35.0	34.0	37.0	27.0	39.0
82-83	32.88013773521806	35.0	34.0	36.0	27.0	39.0
84-85	32.60584891628119	35.0	33.0	36.0	26.5	37.0
86-87	32.32891135502237	35.0	33.0	36.0	26.0	37.0
88-89	32.12245529446752	35.0	33.0	35.0	26.0	37.0
90-91	31.97300182617071	35.0	33.0	35.0	26.0	36.0
92-93	31.81490672608936	35.0	33.0	35.0	26.0	36.0
94-95	31.63377452802832	35.0	33.0	35.0	25.0	36.0
96-97	31.468571822770848	35.0	33.0	35.0	25.0	36.0
98-99	31.291146829958706	35.0	33.0	35.0	25.0	35.0
100	31.18101035560558	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3391424351809853
1101	2	0.39070678122411096
1101	3	0.46942942715212865
1101	4	0.36144350051544905
1101	5	0.302820129481006
1101	6	0.29558945613207044
1101	7	0.2945191532118443
1101	8	0.31853961364407724
1101	9	0.32391442805203496
1101	10-11	0.33343656948676426
1101	12-13	0.3002933154898244
1101	14-15	0.45288199057795
1101	16-17	0.44809281371846765
1101	18-19	0.4645977826838674
1101	20-21	0.44003440462973487
1101	22-23	0.4531587691329335
1101	24-25	0.49927285793612697
1101	26-27	0.4493301443517268
1101	28-29	0.4792610907496595
1101	30-31	0.45719307996779435
1101	32-33	0.5174592230340522
1101	34-35	0.5101255951462846
1101	36-37	0.5146136387136409
1101	38-39	0.514935345572141
1101	40-41	0.574590940207365
1101	42-43	0.4782857029568035
1101	44-45	0.5571155352444705
1101	46-47	0.5719446450651446
1101	48-49	0.5645327604577162
1101	50-51	0.5919362073088834
1101	52-53	0.5931614896387529
1101	54-55	0.5477476017453995
1101	56-57	0.5437114270558752
1101	58-59	0.5378914940719568
1101	60-61	0.6454257587337864
1101	62-63	0.589762348851167
1101	64-65	0.574213809591356
1101	66-67	0.6149735785540003
1101	68-69	0.5800207857895074
1101	70-71	0.6032720488120731
1101	72-73	0.5876365189293367
1101	74-75	0.511226628690352
1101	76-77	0.5274557026971465
1101	78-79	0.5080453391343269
1101	80-81	0.550957509208466
1101	82-83	0.536315359896129
1101	84-85	0.5110255664995691
1101	86-87	0.5660223015852992
1101	88-89	0.601582928607165
1101	90-91	0.5609376488718567
1101	92-93	0.5988439020704348
1101	94-95	0.5657700622679194
1101	96-97	0.6070903892479294
1101	98-99	0.6286451179639343
1101	100	0.7122587259943884
1102	1	0.24450961995825082
1102	2	0.34829594844662637
1102	3	0.3886476269553327
1102	4	0.3887640351437298
1102	5	0.33834170979334033
1102	6	0.30149910105186706
1102	7	0.3032951347138848
1102	8	0.289705286518533
1102	9	0.3948020141313222
1102	10-11	0.3455609619021729
1102	12-13	0.3113497226703572
1102	14-15	0.41362241385996157
1102	16-17	0.46848871835980077
1102	18-19	0.4463962580869776
1102	20-21	0.38858512592246797
1102	22-23	0.4099618653717201
1102	24-25	0.4022898717472785
1102	26-27	0.381349994326996
1102	28-29	0.4113744994242552
1102	30-31	0.4754193195442227
1102	32-33	0.46238934407397636
1102	34-35	0.5100862747678292
1102	36-37	0.522073643722031
1102	38-39	0.5475254840837351
1102	40-41	0.531938968086628
1102	42-43	0.5727718992079716
1102	44-45	0.5604987346553116
1102	46-47	0.5597150937776192
1102	48-49	0.5868385343770086
1102	50-51	0.648064442759356
1102	52-53	0.6232852631218364
1102	54-55	0.566779939132374
1102	56-57	0.5685430213970903
1102	58-59	0.5782977328444332
1102	60-61	0.5734523003138037
1102	62-63	0.5730602397498217
1102	64-65	0.5761931837388303
1102	66-67	0.6263402452783637
1102	68-69	0.5641657056783558
1102	70-71	0.5629606910384126
1102	72-73	0.5630755031263632
1102	74-75	0.5741897460551613
1102	76-77	0.520388468720725
1102	78-79	0.5028876979550532
1102	80-81	0.5100420649235318
1102	82-83	0.5467676685444829
1102	84-85	0.49953794543463914
1102	86-87	0.499412546688653
1102	88-89	0.5077882348844227
1102	90-91	0.5333339498183705
1102	92-93	0.553354515663564
1102	94-95	0.5782636166589548
1102	96-97	0.5630957301838606
1102	98-99	0.5859158033439726
1102	100	0.6565843157495763
1103	1	0.26711282983154305
1103	2	0.274045446449378
1103	3	0.39507503885674566
1103	4	0.3384346459838241
1103	5	0.24367228915485128
1103	6	0.26575583457992735
1103	7	0.2585296321359607
1103	8	0.27681991690148067
1103	9	0.29588183059784967
1103	10-11	0.2823949886924453
1103	12-13	0.27281961818889755
1103	14-15	0.3766475600146464
1103	16-17	0.35557257204558823
1103	18-19	0.3516044694852525
1103	20-21	0.37165909689448284
1103	22-23	0.30829970438581
1103	24-25	0.37713625954889807
1103	26-27	0.34563803558300776
1103	28-29	0.33136017775488824
1103	30-31	0.3719450275525631
1103	32-33	0.37119354586660336
1103	34-35	0.3622292841285173
1103	36-37	0.43014127118500056
1103	38-39	0.4594149170098163
1103	40-41	0.4782914215296685
1103	42-43	0.3697086365871556
1103	44-45	0.4439873829487766
1103	46-47	0.4494693973780741
1103	48-49	0.43600670928653784
1103	50-51	0.4152832544556091
1103	52-53	0.47389903081064233
1103	54-55	0.44403344330062566
1103	56-57	0.44944316013464913
1103	58-59	0.43570878691976134
1103	60-61	0.4274329186137251
1103	62-63	0.3510331174679777
1103	64-65	0.42122928113357716
1103	66-67	0.4353022440228216
1103	68-69	0.438039268172254
1103	70-71	0.4708140591242511
1103	72-73	0.5092794411121275
1103	74-75	0.45280899213459236
1103	76-77	0.4057517018577883
1103	78-79	0.3654191817312551
1103	80-81	0.33408938992521797
1103	82-83	0.3308137490870777
1103	84-85	0.4152889995884621
1103	86-87	0.43967669756234784
1103	88-89	0.46087840691299675
1103	90-91	0.43228337467739664
1103	92-93	0.43633333977208366
1103	94-95	0.4503825149132261
1103	96-97	0.4283747787798724
1103	98-99	0.47553450938819
1103	100	0.4831543948431509
1104	1	0.25092038179788645
1104	2	0.2606711656320684
1104	3	0.3508762127277052
1104	4	0.265190456455592
1104	5	0.21422330433356507
1104	6	0.2065062192199676
1104	7	0.18958796897069163
1104	8	0.21035735604375105
1104	9	0.25343129619991345
1104	10-11	0.2403182210833279
1104	12-13	0.27644889503036296
1104	14-15	0.3626504630747718
1104	16-17	0.3491527352611925
1104	18-19	0.3435565747216245
1104	20-21	0.3664041306147894
1104	22-23	0.3485943266519911
1104	24-25	0.3684933542325908
1104	26-27	0.3345445275666492
1104	28-29	0.3612509340333503
1104	30-31	0.41016336473100523
1104	32-33	0.37641334757455525
1104	34-35	0.36700289677489906
1104	36-37	0.34500547446619834
1104	38-39	0.36887069458641264
1104	40-41	0.36728654101319336
1104	42-43	0.32854430809277346
1104	44-45	0.3748203889093418
1104	46-47	0.43281090860418914
1104	48-49	0.4217931276977893
1104	50-51	0.45188567380458977
1104	52-53	0.4028025902100154
1104	54-55	0.35842357483506504
1104	56-57	0.36972648611265413
1104	58-59	0.4096313926938464
1104	60-61	0.39408766178785726
1104	62-63	0.3903204708470298
1104	64-65	0.40939500365205106
1104	66-67	0.4473914646494421
1104	68-69	0.39349669513470786
1104	70-71	0.3803452995192629
1104	72-73	0.43390066733775967
1104	74-75	0.4464870146518152
1104	76-77	0.3727551493518533
1104	78-79	0.35600478201539687
1104	80-81	0.38060231367648356
1104	82-83	0.3959091660373204
1104	84-85	0.3952738255028976
1104	86-87	0.46811838044709475
1104	88-89	0.4338354080888962
1104	90-91	0.38252779709380036
1104	92-93	0.4129184941095261
1104	94-95	0.4134554181650394
1104	96-97	0.42606071420705405
1104	98-99	0.5236118379559436
1104	100	0.5455209440815914
1105	1	0.1954772257416515
1105	2	0.21081939104846725
1105	3	0.28484151978819483
1105	4	0.2346075845348281
1105	5	0.1723278788603011
1105	6	0.19460863067557455
1105	7	0.16933141102689575
1105	8	0.1791294295592607
1105	9	0.20794677217413238
1105	10-11	0.19393584398753205
1105	12-13	0.1995378918316959
1105	14-15	0.26701094994136554
1105	16-17	0.24833588695494058
1105	18-19	0.25344711871574077
1105	20-21	0.25115718689570343
1105	22-23	0.2829236496157961
1105	24-25	0.24163085373630366
1105	26-27	0.22505371539989483
1105	28-29	0.26911821308488015
1105	30-31	0.2503317151398292
1105	32-33	0.2655891680270983
1105	34-35	0.2848197959408978
1105	36-37	0.2579789547745506
1105	38-39	0.27597457032516814
1105	40-41	0.24687431146497119
1105	42-43	0.2371908062111885
1105	44-45	0.2881481336574012
1105	46-47	0.3448524426972668
1105	48-49	0.33629539028034827
1105	50-51	0.3583202166877726
1105	52-53	0.31641763336082107
1105	54-55	0.2935476368007883
1105	56-57	0.27854002690838797
1105	58-59	0.34852399387700217
1105	60-61	0.3709233822083533
1105	62-63	0.39118247170495124
1105	64-65	0.40403398069794605
1105	66-67	0.37113988965072764
1105	68-69	0.3660490085675008
1105	70-71	0.38932826222267636
1105	72-73	0.3820917750636639
1105	74-75	0.3892544121979
1105	76-77	0.3627074546580715
1105	78-79	0.3413376457430317
1105	80-81	0.3377113884350109
1105	82-83	0.3142992938457283
1105	84-85	0.33267468518263854
1105	86-87	0.40432105148602204
1105	88-89	0.34033533226413226
1105	90-91	0.37495989008814945
1105	92-93	0.38381718962993006
1105	94-95	0.3398745450724512
1105	96-97	0.36814942362018144
1105	98-99	0.44490479053832743
1105	100	0.4556969693043058
1106	1	0.17631464829900523
1106	2	0.25732575751335673
1106	3	0.3078691160728795
1106	4	0.2720718754720579
1106	5	0.20476572617617705
1106	6	0.22658759688305707
1106	7	0.22242097575954034
1106	8	0.22856572421462573
1106	9	0.21730331836344874
1106	10-11	0.2382811970110481
1106	12-13	0.1737151054046393
1106	14-15	0.33257598976994984
1106	16-17	0.31831141773114524
1106	18-19	0.30483661865457634
1106	20-21	0.24586130811949403
1106	22-23	0.27185229462185845
1106	24-25	0.2889270866962974
1106	26-27	0.276129522563096
1106	28-29	0.31830908981648065
1106	30-31	0.33312217102690056
1106	32-33	0.4143823935159503
1106	34-35	0.33176651754433095
1106	36-37	0.32924210186723
1106	38-39	0.3688219512975479
1106	40-41	0.3794498108309483
1106	42-43	0.38426177668377903
1106	44-45	0.434963532746508
1106	46-47	0.4047323485583121
1106	48-49	0.3820825704074693
1106	50-51	0.48344780023150236
1106	52-53	0.46990026799692686
1106	54-55	0.4491638388074719
1106	56-57	0.463421155892874
1106	58-59	0.43904820799513544
1106	60-61	0.46145185934660304
1106	62-63	0.503479751535771
1106	64-65	0.49566542857475326
1106	66-67	0.5050078722385223
1106	68-69	0.4561105340298397
1106	70-71	0.4808701252646159
1106	72-73	0.46137971211484086
1106	74-75	0.505477288745098
1106	76-77	0.4680297938416018
1106	78-79	0.5052445519052426
1106	80-81	0.4491077022460246
1106	82-83	0.4654488282277214
1106	84-85	0.415373196988412
1106	86-87	0.4822268232447655
1106	88-89	0.5195801959236448
1106	90-91	0.4938974609269593
1106	92-93	0.49465660869318384
1106	94-95	0.519635490051666
1106	96-97	0.5404726932372768
1106	98-99	0.5614070202872128
1106	100	0.5872400552649708
1107	1	0.13484270960839595
1107	2	0.15994320872415813
1107	3	0.22802968584981897
1107	4	0.20961379718917073
1107	5	0.18726708943201942
1107	6	0.17753598772914359
1107	7	0.18357618467689463
1107	8	0.2023256094195247
1107	9	0.1630094144851526
1107	10-11	0.18365567954985096
1107	12-13	0.12004434705038136
1107	14-15	0.16859559313290617
1107	16-17	0.1397444236270644
1107	18-19	0.2094852730634642
1107	20-21	0.22388928814554987
1107	22-23	0.22872956831498925
1107	24-25	0.24631502130610272
1107	26-27	0.23395211497647495
1107	28-29	0.2702364807624136
1107	30-31	0.276954030379855
1107	32-33	0.2515297230705684
1107	34-35	0.23786339127227762
1107	36-37	0.2712120449560942
1107	38-39	0.25953299482189607
1107	40-41	0.27820670391329116
1107	42-43	0.2426077345342108
1107	44-45	0.30513413493437014
1107	46-47	0.315181480554763
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2216	94-95	-1.2406227959940281
2216	96-97	-1.3584476940646866
2216	98-99	-1.3673894305746046
2216	100	-1.3636694255172763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	444.0
3	36.0
4	55.0
5	120.0
6	385.0
7	1101.0
8	2274.0
9	3862.0
10	5610.0
11	8110.0
12	11483.0
13	16309.0
14	19318.0
15	24470.0
16	31633.0
17	43616.0
18	44067.0
19	24390.0
20	17755.0
21	16942.0
22	17639.0
23	19490.0
24	22453.0
25	27632.0
26	35381.0
27	45676.0
28	59748.0
29	78572.0
30	101676.0
31	130875.0
32	169079.0
33	220391.0
34	301789.0
35	439844.0
36	713989.0
37	1300449.0
38	1795764.0
39	724629.0
40	2623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30419776901592	20.739640232700314	14.892915883558425	23.063246114725338
2	15.64505169176341	20.76873181134995	39.50911614330322	24.077100353583422
3	17.72659347063133	25.253326670449777	31.3874132923601	25.632666566558797
4	12.769191595505426	17.780226558197917	37.894404893825175	31.556176952471482
5	12.530451419481778	37.37243584965267	35.42984170431956	14.667271026545997
6	29.951856558936168	38.30250193106205	17.467434307236797	14.278207202764987
7	25.944464224230863	33.161488400891464	22.580593884357544	18.313453490520132
8	24.62719218035338	36.14114217695043	20.4825269893771	18.749138653319093
9	24.372032741755493	16.89574958751802	22.124467006611596	36.60775066411489
10-11	23.553713077453374	27.066441099937204	28.758044958708602	20.62180086390082
12-13	24.457407367682062	24.65244023737603	29.164354951643425	21.725797443298486
14-15	21.69893151334592	26.66980313915266	27.12447075885015	24.506794588651275
16-17	20.420903566364938	29.879273340546654	27.90365078270081	21.7961723103876
18-19	20.249745396338305	27.968545972724883	31.092319542372394	20.68938908856442
20-21	21.32124137630892	27.24851802072294	31.02438407828536	20.40585652468278
22-23	21.02691352457429	27.156916260820946	31.08035135691135	20.735818857693413
24-25	20.86847975030862	27.14155284544188	31.123509359028432	20.866458045221066
26-27	20.692259601131475	27.111211836265348	31.19024105978089	21.006287502822286
28-29	20.73912263110971	27.149349758452168	31.284545553175636	20.826982057262494
30-31	21.090041340887737	27.50334604618682	30.783509171966905	20.623103440958538
32-33	20.658153227248306	27.30661013709558	31.174951403823943	20.860285231832176
34-35	20.82891760533199	27.047651588913585	31.082048971870368	21.041381833884053
36-37	21.25961023686513	27.284623204328483	30.59364514816243	20.86212141064395
38-39	21.099517429798606	27.40709840718962	30.67697489335506	20.81640926965672
40-41	20.964526174830574	27.23593252073135	30.68699853804486	21.11254276639321
42-43	20.938398337325044	27.388555821978215	30.496989125541557	21.17605671515518
44-45	21.224963458838005	27.49971873606702	30.221497083420335	21.053820721674636
46-47	21.242742117317846	27.79947123923886	29.738818851983257	21.218967791460038
48-49	21.301062907591565	27.649170583913186	29.58500722026508	21.464759288230173
50-51	21.365425663833037	27.66844623012961	29.382412307770185	21.583715798267168
52-53	21.338248391625573	27.843894736143564	29.271550334515027	21.546306537715836
54-55	21.414016033818957	28.145136510620354	29.118957898994687	21.321889556565996
56-57	21.216969235667385	27.893465092946734	29.175473044266543	21.71409262711934
58-59	21.11103857651944	27.543791988075935	29.532320102252168	21.81284933315246
60-61	21.39605218097995	27.823446192319096	29.189185143276386	21.591316483424563
62-63	21.19258531171066	27.837366634982995	29.302500941790484	21.667547111515866
64-65	21.155847380711297	27.594893203814575	29.483026859818214	21.76623255565592
66-67	21.09965632556798	27.614570104475856	29.330056319147907	21.955717250808256
68-69	21.427567741208147	27.820955931687042	29.087654580393146	21.663821746711662
70-71	21.393335997045533	27.567993723145857	29.179570469463073	21.859099810345544
72-73	21.239454917442668	27.729197696367365	29.284166700233143	21.747180685956817
74-75	21.2788241516285	27.747292728544114	29.106040592442927	21.86784252738446
76-77	21.283091338320926	27.79729520551867	28.92827715693941	21.991336299220997
78-79	21.37665631480364	27.80791648217942	28.992336184969886	21.82309101804706
80-81	21.23085933521203	27.683998851791607	29.073986906720418	22.011154906275944
82-83	21.11700348359462	27.705523015684086	29.16691198570295	22.01056151501835
84-85	21.20193762684849	27.63101382028338	28.963996518963363	22.203052033904765
86-87	21.280685453761325	27.733158365804723	28.903841448306423	22.08231473212753
88-89	21.096728183659884	27.606238771617026	28.96055094138238	22.336482103340707
90-91	20.96507030524534	27.51084815545476	28.927563165610366	22.596518373689538
92-93	21.039651233414478	27.69312837012643	28.80929472179334	22.457925674665756
94-95	20.964068266504846	27.437836192766735	28.961294035952186	22.636801504776233
96-97	20.877949422953623	27.447009909519434	28.97859824372459	22.696442423802353
98-99	20.885257225824255	27.438084867206324	28.897455675252896	22.779202231716525
100	20.95763553488214	27.375603914127485	28.81206493297045	22.854695618019928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1046.0
1	871.0
2	2000.0
3	3238.5
4	2040.0
5	1036.5
6	2226.5
7	5703.5
8	9247.0
9	10408.0
10	8156.5
11	5218.5
12	4718.0
13	5159.5
14	5796.5
15	6538.0
16	7318.5
17	8189.0
18	9249.0
19	10399.5
20	12055.0
21	14628.0
22	18474.0
23	24380.5
24	31612.5
25	40258.0
26	52318.0
27	67564.0
28	86092.5
29	105632.0
30	122569.0
31	138481.5
32	153459.5
33	167577.5
34	181508.5
35	193059.0
36	202275.0
37	210349.0
38	217461.5
39	221731.5
40	223464.5
41	223156.0
42	220331.0
43	217614.5
44	214469.5
45	210584.5
46	206858.5
47	201373.0
48	195643.5
49	193387.5
50	190040.0
51	183133.0
52	173751.5
53	166530.0
54	161968.0
55	154407.0
56	143451.5
57	129327.0
58	116559.5
59	106590.5
60	94202.5
61	79241.0
62	66122.0
63	54262.0
64	43238.5
65	34136.0
66	26705.5
67	20823.5
68	15775.5
69	12001.0
70	8937.5
71	6433.0
72	4899.5
73	3605.5
74	2572.0
75	1783.5
76	1123.0
77	666.0
78	397.5
79	245.0
80	157.0
81	69.0
82	35.5
83	23.0
84	16.0
85	10.5
86	8.5
87	5.0
88	2.0
89	2.0
90	1.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.5
99	5.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05991037518988209
2	3.086572652750237E-4
3	3.086572652750237E-5
4	0.11610143033320014
5	0.0014815548733201134
6	0.0012963605141550994
7	0.0
8	0.0
9	3.086572652750237E-5
10-11	0.0
12-13	3.086572652750237E-5
14-15	7.716431631875592E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.5432863263751184E-4
30-31	2.5464224385189453E-4
32-33	3.858215815937796E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8519435916501418E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	7.716431631875592E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.2346290611000947E-4
80-81	2.932244020112725E-4
82-83	3.3180656017065045E-4
84-85	0.0
86-87	7.716431631875592E-6
88-89	9.259717958250709E-5
90-91	9.259717958250709E-5
92-93	6.867624152369277E-4
94-95	6.867624152369277E-4
96-97	7.716431631875592E-6
98-99	4.6298589791253545E-5
100	0.0011420318815175874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6479679.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.4048953482896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64444271642495	44.93650203248911
2	14.55877264240692	17.297247304434492
3	4.473109248535918	7.971737603722278
4	1.8646256924224336	4.430715764883468
5	0.9821543567878035	2.9172388390423074
6	0.5628897316156074	2.0063043359551185
7	0.37037778601728316	1.5401577532381532
8	0.2706409161691737	1.2861916241595983
9	0.20983154527278125	1.1218518888929516
>10	0.9874071018506339	10.365566713457785
>50	0.05258122290194642	2.1133243210816626
>100	0.02179087570771967	2.2231892350553695
>500	8.828396220979438E-4	0.35170187570413836
>1k	3.8946652528894216E-4	0.44452387067976373
>5k	2.5964434864818276E-5	0.09546003562821329
>10k+	7.789330459445482E-5	0.8982868015756201
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22305	0.34423001509797013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22222	0.34294908744707875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13712	0.21161542107255624	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5432863263751184E-5	0.0	0.0	0.0
3	0.0	4.6298589791253545E-5	0.0	0.0	0.0
4	0.0	4.6298589791253545E-5	0.0	0.0	0.0
5	0.0	6.173145305500474E-5	0.0	0.0	0.0
6	0.0	6.173145305500474E-5	0.0	0.0	0.0
7	0.0	6.173145305500474E-5	0.0	0.0	0.0
8	0.0	6.173145305500474E-5	0.0	0.0	0.0
9	0.0	6.173145305500474E-5	0.0	1.5432863263751184E-5	0.0
10-11	0.0	6.173145305500474E-5	0.0	4.6298589791253545E-5	0.0
12-13	0.0	6.173145305500474E-5	0.0	4.6298589791253545E-5	0.0
14-15	0.0	1.003136112143827E-4	0.0	5.4015021423129144E-5	0.0
16-17	0.0	1.3117933774188506E-4	0.0	6.173145305500474E-5	0.0
18-19	7.716431631875592E-6	1.3889576937376065E-4	0.0	6.173145305500474E-5	0.0
20-21	1.5432863263751184E-5	1.3889576937376065E-4	0.0	1.0803004284625829E-4	0.0
22-23	1.5432863263751184E-5	1.3889576937376065E-4	0.0	2.4692581222001894E-4	0.0
24-25	1.5432863263751184E-5	1.3889576937376065E-4	0.0	6.018816672862962E-4	0.0
26-27	1.5432863263751184E-5	1.3889576937376065E-4	0.0	0.0010725839968307072	0.0
28-29	1.5432863263751184E-5	1.5432863263751184E-4	0.0	0.002947676883376476	0.0
30-31	1.5432863263751184E-5	1.5432863263751184E-4	0.0	0.00782446167472185	0.0
32-33	1.5432863263751184E-5	1.5432863263751184E-4	0.0	0.020332797349992184	0.0
34-35	3.086572652750237E-5	1.5432863263751184E-4	0.0	0.04018717593880808	0.0
36-37	3.086572652750237E-5	1.6976149590126302E-4	0.0	0.07031984146128226	0.0
38-39	3.086572652750237E-5	1.9291079079688977E-4	0.0	0.11360130648447245	0.0
40-41	3.086572652750237E-5	2.0062722242876536E-4	0.0	0.161389167580678	0.0
42-43	3.086572652750237E-5	2.0062722242876536E-4	0.0	0.21206297410720498	0.0
44-45	3.086572652750237E-5	2.0062722242876536E-4	0.0	0.2740413529744298	0.0
46-47	3.086572652750237E-5	2.0062722242876536E-4	0.0	0.3519387302982139	0.0
48-49	3.086572652750237E-5	2.1606008569251655E-4	0.0	0.43759112141203294	0.0
50-51	3.086572652750237E-5	2.3149294895626773E-4	0.0	0.5260445772082228	0.0
52-53	3.086572652750237E-5	2.3149294895626773E-4	0.0	0.6144594508462533	0.0
54-55	3.086572652750237E-5	2.3149294895626773E-4	0.0	0.7167098864002368	0.0
56-57	3.086572652750237E-5	2.3149294895626773E-4	0.0	0.8403425540061475	0.0
58-59	3.086572652750237E-5	2.3149294895626773E-4	0.0	0.9795083984870239	0.0
60-61	3.086572652750237E-5	2.3149294895626773E-4	0.0	1.124075745110213	0.0
62-63	3.086572652750237E-5	2.3149294895626773E-4	0.0	1.273496727229852	0.0
64-65	3.086572652750237E-5	2.3149294895626773E-4	0.0	1.4460129892236946	0.0
66-67	3.086572652750237E-5	2.3149294895626773E-4	0.0	1.6453129854117774	0.0
68-69	3.086572652750237E-5	2.3149294895626773E-4	0.0	1.870362713955429	0.0
70-71	3.086572652750237E-5	2.3149294895626773E-4	0.0	2.1155909112164353	0.0
72-73	3.858215815937796E-5	2.700751071156457E-4	0.0	2.37854375193586	0.0
74-75	4.6298589791253545E-5	2.855079703793969E-4	0.0	2.6486883069361924	0.0
76-77	4.6298589791253545E-5	2.932244020112725E-4	0.0	2.9423062469606904	0.0
78-79	4.6298589791253545E-5	3.009408336431481E-4	0.0	3.2825082847468217	0.0
80-81	4.6298589791253545E-5	3.2409012853877486E-4	0.0	3.6507518350831885	0.0
82-83	4.6298589791253545E-5	3.2409012853877486E-4	0.0	4.035878937830099	0.0
84-85	6.173145305500474E-5	3.3952299180252605E-4	0.0	4.4257439295989816	0.0
86-87	6.173145305500474E-5	3.626722866981528E-4	0.0	4.845857333364816	0.0
88	6.173145305500474E-5	4.012544448575307E-4	0.0	5.187988479058916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13360	0.0	59.336464	1
GTATCAA	23175	0.0	39.040886	1
GTGGTAT	5100	0.0	33.136776	1
ATCAACG	29150	0.0	30.7452	3
TCAACGC	29365	0.0	30.568157	4
TATCAAC	30070	0.0	29.883	2
CAACGCA	30480	0.0	29.42039	5
TGGTATC	5740	0.0	28.276876	2
AACGCAG	32090	0.0	28.031769	6
CGCAGAG	36015	0.0	24.754768	8
ACGCAGA	36260	0.0	24.691198	7
GCAGAGT	38570	0.0	22.798124	9
GAGTACT	28940	0.0	21.817959	12-13
GTACATG	16830	0.0	21.733162	1
CAGAGTA	37860	0.0	21.251917	10-11
TACATGG	17120	0.0	20.445297	2
AGAGTAC	36180	0.0	20.225302	10-11
ACATGGG	17180	0.0	19.798672	3
GTACTTT	32105	0.0	19.418226	14-15
AGTACTT	30350	0.0	19.06226	12-13
>>END_MODULE