FastQCFastQC Report
Wed 25 May 2016
SRR2049532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8740983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86400.046.096521
GTATCAA139450.037.2973021
ATCAACG147050.033.657743
TCAACGC147900.033.4643064
CAACGCA162900.030.4142995
TATCAAC171300.029.5503372
AACGCAG177300.028.000756
ACGCAGA201450.024.1865277
GTACATG238050.023.891761
CGCAGAG204350.023.4836048
TACATGG237550.023.0112272
GTGGTAT49600.022.3965071
ACATGGG244100.021.5340733
TAGACCG4300.021.4253335
GCAGAGT234250.019.5593269
CATGGGG168600.018.0019954
TGGTATC60800.017.4257052
CAGAGTA225750.017.03011910-11
GAGTACA184400.017.0177361
AGAGTAC203300.016.87503610-11
Produced by FastQC (version 0.11.5)