##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049532_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8740983 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.842423901293483 31.0 31.0 34.0 28.0 34.0 2 31.042878815803668 31.0 31.0 34.0 28.0 34.0 3 31.153724586811347 31.0 31.0 34.0 28.0 34.0 4 34.749962675822616 37.0 35.0 37.0 32.0 37.0 5 34.77114622005328 37.0 35.0 37.0 32.0 37.0 6 34.76297185339452 37.0 35.0 37.0 32.0 37.0 7 30.7956904846972 37.0 33.0 37.0 2.0 37.0 8 32.56424843750411 37.0 33.0 37.0 17.0 37.0 9 35.36560441771824 39.0 34.0 39.0 28.0 39.0 10-11 35.898112717986066 39.0 36.0 39.0 31.0 39.0 12-13 36.050704709069905 39.0 37.0 39.0 31.0 39.0 14-15 37.23991563649077 40.0 37.0 41.0 31.0 41.0 16-17 37.2607388093536 40.0 37.0 41.0 31.0 41.0 18-19 37.271472842356516 40.0 37.0 41.0 31.0 41.0 20-21 37.33787138128515 40.0 37.0 41.0 31.0 41.0 22-23 37.30087874555986 40.0 37.0 41.0 31.0 41.0 24-25 37.276188959525484 40.0 37.0 41.0 31.0 41.0 26-27 37.29121026776966 40.0 37.0 41.0 31.0 41.0 28-29 37.26395320755114 40.0 37.0 41.0 31.0 41.0 30-31 37.17485327451158 40.0 37.0 41.0 31.0 41.0 32-33 37.10411986844042 40.0 37.0 41.0 31.0 41.0 34-35 37.02807132790443 40.0 37.0 41.0 30.0 41.0 36-37 36.858994749217565 40.0 37.0 41.0 30.0 41.0 38-39 36.738406767293796 40.0 37.0 41.0 30.0 41.0 40-41 36.65108152023634 40.0 36.5 41.0 30.0 41.0 42-43 36.53772253074969 40.0 36.0 41.0 30.0 41.0 44-45 36.43047984420059 40.0 36.0 41.0 30.0 41.0 46-47 36.274454486411884 40.0 36.0 41.0 29.5 41.0 48-49 36.190210242944076 40.0 35.5 41.0 29.0 41.0 50-51 36.05335040692792 39.0 35.0 41.0 29.0 41.0 52-53 35.92652885836753 39.0 35.0 41.0 28.5 41.0 54-55 35.80172224336782 39.0 35.0 41.0 28.0 41.0 56-57 35.599733920086564 39.0 35.0 41.0 28.0 41.0 58-59 35.41121833780022 39.0 35.0 41.0 28.0 41.0 60-61 35.18278550593223 38.5 34.5 40.0 27.5 41.0 62-63 34.93369143950972 38.0 34.0 40.0 27.0 41.0 64-65 34.56892971877419 37.5 34.0 40.0 26.5 41.0 66-67 31.08188146573446 36.0 30.0 40.0 6.5 41.0 68-69 31.422541034572426 36.0 30.0 39.0 11.0 41.0 70-71 31.563543882879078 35.5 30.0 39.0 15.5 41.0 72-73 32.7672673656956 35.0 31.5 39.0 22.5 40.5 74-75 32.81957126561166 35.0 33.0 38.5 24.0 40.0 76-77 32.4843609122681 35.0 33.0 37.5 22.5 39.5 78-79 29.077491227245265 35.0 29.0 37.0 6.0 39.0 80-81 30.808255661863203 35.0 29.5 36.0 17.0 39.0 82-83 31.14770621336296 35.0 31.5 36.0 18.5 38.0 84-85 29.28130600414164 34.5 29.5 35.5 8.5 37.0 86-87 28.859032273601265 34.0 26.5 35.0 7.0 37.0 88-89 29.986446032442807 34.0 30.5 35.0 3.5 36.0 90-91 30.03821217819552 35.0 31.0 35.0 2.0 36.0 92-93 29.793169143562 35.0 31.0 35.0 2.0 36.0 94-95 29.498117774625577 34.5 31.0 35.0 2.0 36.0 96-97 29.217571353244825 34.0 31.0 35.0 2.0 35.0 98-99 28.918890758625203 34.0 30.5 35.0 2.0 35.0 100 28.632351990617074 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34037146592052636 1101 2 0.35356965598573353 1101 3 0.4007944969491959 1101 4 0.3922585486000898 1101 5 0.3708720016316107 1101 6 0.40796372208637877 1101 7 -12.059218947163828 1101 8 -6.485085917204717 1101 9 -2.1429506095856468 1101 10-11 -0.08156520999192196 1101 12-13 0.37842377785633374 1101 14-15 0.5840515420629231 1101 16-17 0.6377854279491189 1101 18-19 0.6614225697577325 1101 20-21 0.6685475968357082 1101 22-23 0.5894623294654835 1101 24-25 0.6387161545197415 1101 26-27 0.595242112868938 1101 28-29 0.5300237689745728 1101 30-31 0.6077549780512328 1101 32-33 0.6378851430884609 1101 34-35 0.6441131549632146 1101 36-37 0.6716664305980231 1101 38-39 0.6966105593565217 1101 40-41 0.6970190710120079 1101 42-43 0.6660821677217683 1101 44-45 0.5695596811454635 1101 46-47 0.5736461252963707 1101 48-49 0.5314004706726507 1101 50-51 0.5975565250372483 1101 52-53 0.6375551979269218 1101 54-55 0.5697877696232183 1101 56-57 0.5508762101679707 1101 58-59 0.47113634968000895 1101 60-61 0.5336404776565047 1101 62-63 0.556532952711585 1101 64-65 0.45309828427450327 1101 66-67 -10.10968532407572 1101 68-69 -7.9760817050607 1101 70-71 -6.521660160723005 1101 72-73 -1.4069395283960233 1101 74-75 -0.1464090087183152 1101 76-77 0.12770075089649424 1101 78-79 3.2396454304962745 1101 80-81 1.1492671467068334 1101 82-83 0.5288482511676875 1101 84-85 -5.132943391441447 1101 86-87 -5.619609586935699 1101 88-89 -1.160758259249281 1101 90-91 0.1319976343715652 1101 92-93 0.5201168839004353 1101 94-95 0.5388037221368762 1101 96-97 0.6495860619839142 1101 98-99 0.6100811173814868 1101 100 0.644264196695314 1102 1 0.2733596785039829 1102 2 0.2917493658763881 1102 3 0.29157336730072814 1102 4 0.2479479788260619 1102 5 0.28442071011367176 1102 6 0.32965837983716284 1102 7 -12.174412882283153 1102 8 -6.593531497687593 1102 9 -2.239253262837103 1102 10-11 -0.20648006369673766 1102 12-13 0.38017865753581503 1102 14-15 0.5118965119716634 1102 16-17 0.563614303410688 1102 18-19 0.5517484316731398 1102 20-21 0.5420121653737198 1102 22-23 0.5392910649674363 1102 24-25 0.559936345733945 1102 26-27 0.5003756082245445 1102 28-29 0.5167104266911764 1102 30-31 0.5301112237282268 1102 32-33 0.496682307947232 1102 34-35 0.5379817523341899 1102 36-37 0.6321101292269873 1102 38-39 0.6777402720450212 1102 40-41 0.5875864793045906 1102 42-43 0.5592108228666604 1102 44-45 0.5846098924251777 1102 46-47 0.5698634029364555 1102 48-49 0.4899545425746865 1102 50-51 0.5618546572173528 1102 52-53 0.5701591662302334 1102 54-55 0.498960068862182 1102 56-57 0.554202568769341 1102 58-59 0.48576533138710687 1102 60-61 0.48985056367101265 1102 62-63 0.45428317924342565 1102 64-65 0.41939788930915967 1102 66-67 -10.183252923422152 1102 68-69 -6.556200124796053 1102 70-71 -6.089102362638709 1102 72-73 -0.92908992427434 1102 74-75 -0.013867608429350753 1102 76-77 0.11568989996179369 1102 78-79 3.303756834838758 1102 80-81 1.245366749229138 1102 82-83 0.572255019871605 1102 84-85 -5.280205079343368 1102 86-87 -5.736043468720073 1102 88-89 -1.2066810286048337 1102 90-91 0.09933314685737571 1102 92-93 0.4808335148320495 1102 94-95 0.5065772776453947 1102 96-97 0.5634638836980308 1102 98-99 0.5940352348913542 1102 100 0.7297170265288209 1103 1 0.29792268238120556 1103 2 0.2752296957153355 1103 3 0.256323248819033 1103 4 0.2664128609406333 1103 5 0.31317490757787425 1103 6 0.30370329858974543 1103 7 -12.20539986007812 1103 8 -6.599358772723093 1103 9 -2.2125516479217424 1103 10-11 -0.17282202232120625 1103 12-13 0.2841149779719174 1103 14-15 0.48774054826115787 1103 16-17 0.43601480189187924 1103 18-19 0.5223489105283932 1103 20-21 0.5307133904106109 1103 22-23 0.5327826027123805 1103 24-25 0.593889656246624 1103 26-27 0.5824330356514835 1103 28-29 0.5597268715539485 1103 30-31 0.5376112138272475 1103 32-33 0.5796329135129881 1103 34-35 0.5797557345907549 1103 36-37 0.6151679073685301 1103 38-39 0.6364189844603629 1103 40-41 0.5970007683066285 1103 42-43 0.5902987906273225 1103 44-45 0.5673047447532014 1103 46-47 0.5919009976062952 1103 48-49 0.5075267966047079 1103 50-51 0.5448103452846595 1103 52-53 0.519342951686177 1103 54-55 0.542045160499022 1103 56-57 0.5508122542130991 1103 58-59 0.5530095770492736 1103 60-61 0.5241301663822071 1103 62-63 0.437648437727141 1103 64-65 0.3575918102852782 1103 66-67 -10.25873401189596 1103 68-69 -8.138609806983492 1103 70-71 -6.643740794359154 1103 72-73 -1.4423791256527352 1103 74-75 -0.2471682905406638 1103 76-77 0.07000052133390966 1103 78-79 3.191400547520324 1103 80-81 1.090487525747367 1103 82-83 0.41712431157331054 1103 84-85 -5.390741787105689 1103 86-87 -5.854243312680676 1103 88-89 -1.3025232563158653 1103 90-91 -0.0347802074484278 1103 92-93 0.3876312176293588 1103 94-95 0.49249335784418946 1103 96-97 0.5488857951322537 1103 98-99 0.62800537706498 1103 100 0.7045274008165556 1104 1 0.22312426859205914 1104 2 0.23853573748706935 1104 3 0.2872956635633841 1104 4 0.2854110202718303 1104 5 0.2769023891213038 1104 6 0.3180788967598147 1104 7 -12.17356651413747 1104 8 -6.5636820510896605 1104 9 -2.242013433449138 1104 10-11 -0.23126105356131887 1104 12-13 0.3773820436297157 1104 14-15 0.5283550602075593 1104 16-17 0.5231850284713815 1104 18-19 0.5384150488779653 1104 20-21 0.4920403250846874 1104 22-23 0.5111888636853905 1104 24-25 0.6684100399770188 1104 26-27 0.5948261182488537 1104 28-29 0.568383896400249 1104 30-31 0.5140811783798043 1104 32-33 0.5355717066212193 1104 34-35 0.5687599998791413 1104 36-37 0.6742179726416992 1104 38-39 0.6953801237155233 1104 40-41 0.6639229806313978 1104 42-43 0.6473729554223269 1104 44-45 0.6173865816045065 1104 46-47 0.6603695618119687 1104 48-49 0.6223502234100806 1104 50-51 0.6155717520087904 1104 52-53 0.5942755013678394 1104 54-55 0.6055853985330799 1104 56-57 0.6033145408903664 1104 58-59 0.588677734240342 1104 60-61 0.5256232399341485 1104 62-63 0.4903377291475053 1104 64-65 0.351856754014527 1104 66-67 -10.260985993085058 1104 68-69 -8.175793218767115 1104 70-71 -6.596671253555602 1104 72-73 -1.5381372444384205 1104 74-75 -0.3370017126558267 1104 76-77 -0.019395318333359057 1104 78-79 3.1237087522837967 1104 80-81 1.0355894905953882 1104 82-83 0.36077758801982895 1104 84-85 -5.349013257482145 1104 86-87 -5.786883696694353 1104 88-89 -1.358064362859352 1104 90-91 -0.02303704202914858 1104 92-93 0.3138285572378834 1104 94-95 0.37607998412775245 1104 96-97 0.44470503462315136 1104 98-99 0.4691870961687563 1104 100 0.5528424641469449 1105 1 0.1850386181612187 1105 2 0.20530381374731022 1105 3 0.19006956772868477 1105 4 0.22077469000015526 1105 5 0.24546090720671287 1105 6 0.27891699581295626 1105 7 -12.204614698958554 1105 8 -6.634095530457351 1105 9 -2.3978399446805057 1105 10-11 -0.3425251284813342 1105 12-13 0.18592579834032819 1105 14-15 0.3613933404225236 1105 16-17 0.41284519948020915 1105 18-19 0.4235454854298979 1105 20-21 0.43456437836944417 1105 22-23 0.4501438371625497 1105 24-25 0.47601096624981665 1105 26-27 0.44265150419921895 1105 28-29 0.44640486782322597 1105 30-31 0.4806138332429626 1105 32-33 0.488408419330689 1105 34-35 0.5120449276346974 1105 36-37 0.591258638678255 1105 38-39 0.5322657227763941 1105 40-41 0.5323986320432965 1105 42-43 0.49613239675984033 1105 44-45 0.447548830605065 1105 46-47 0.4449916051202294 1105 48-49 0.44019861626563284 1105 50-51 0.5300486424488255 1105 52-53 0.5225835319383094 1105 54-55 0.5149814601571379 1105 56-57 0.4500487856678532 1105 58-59 0.46360021094851334 1105 60-61 0.4432127874594016 1105 62-63 0.35266058131086453 1105 64-65 0.2378348599758553 1105 66-67 -10.278683225045278 1105 68-69 -8.285469220258467 1105 70-71 -6.689986535170078 1105 72-73 -1.6184355877682535 1105 74-75 -0.4158046261960422 1105 76-77 -0.1688062869727176 1105 78-79 3.0314511162675544 1105 80-81 0.9581610048517177 1105 82-83 0.23880539413106305 1105 84-85 -5.457163218744025 1105 86-87 -5.928305827925836 1105 88-89 -1.4454417009333724 1105 90-91 -0.16764930882006013 1105 92-93 0.17085320948782723 1105 94-95 0.19897406877901958 1105 96-97 0.25185654653013856 1105 98-99 0.302918262513014 1105 100 0.34555909765827764 1106 1 0.06705802085590662 1106 2 0.08381931630785289 1106 3 0.08730822070797828 1106 4 0.07799248929064362 1106 5 0.06460823537189953 1106 6 0.11671019016144157 1106 7 -12.281415875297725 1106 8 -6.724225909310679 1106 9 -2.603251422964746 1106 10-11 -0.5082681339425363 1106 12-13 0.05197097167219766 1106 14-15 0.20530851453376187 1106 16-17 0.23931656338638874 1106 18-19 0.2435765018216003 1106 20-21 0.20392565136090468 1106 22-23 0.2165637781140859 1106 24-25 0.29034623022400297 1106 26-27 0.2679408838352728 1106 28-29 0.2577928979137738 1106 30-31 0.28635858564991423 1106 32-33 0.3072952232520052 1106 34-35 0.3304886798765807 1106 36-37 0.3934982126715312 1106 38-39 0.33405659890242845 1106 40-41 0.35084572956986193 1106 42-43 0.28089562697456927 1106 44-45 0.21779358512252855 1106 46-47 0.33961330988426397 1106 48-49 0.26638355852895756 1106 50-51 0.3075276974854262 1106 52-53 0.34080348191503873 1106 54-55 0.3086538906205831 1106 56-57 0.28030760474123895 1106 58-59 0.25882268858971713 1106 60-61 0.21631368076682378 1106 62-63 0.14954435452393255 1106 64-65 -0.04237753198558636 1106 66-67 -10.430909186177658 1106 68-69 -8.40788694333521 1106 70-71 -6.9045356939272615 1106 72-73 -1.8894988491360074 1106 74-75 -0.6244693421610279 1106 76-77 -0.3479872085410989 1106 78-79 2.7366122367096857 1106 80-81 0.6227352983917775 1106 82-83 0.013398327923752618 1106 84-85 -5.593089059950945 1106 86-87 -6.03399417937694 1106 88-89 -1.6317389242230647 1106 90-91 -0.4083691192195502 1106 92-93 -0.08498440475826996 1106 94-95 -0.04653250675607623 1106 96-97 -0.007717272033890055 1106 98-99 0.0021687544711248563 1106 100 0.09638034057067912 1107 1 0.07594834782717896 1107 2 0.11094921468784946 1107 3 0.14576975154496452 1107 4 0.06128091245616929 1107 5 0.07185946458307058 1107 6 0.11788991117474268 1107 7 -12.250790520303177 1107 8 -6.76637522383222 1107 9 -2.5696495770924415 1107 10-11 -0.5502626335124106 1107 12-13 0.10107068125945062 1107 14-15 0.18106708002650862 1107 16-17 0.2588248334175063 1107 18-19 0.2757132051382172 1107 20-21 0.20448714861913686 1107 22-23 0.29188325211003274 1107 24-25 0.3573331094739416 1107 26-27 0.2382797991797858 1107 28-29 0.2587276194195738 1107 30-31 0.2599114346113751 1107 32-33 0.2386150457885421 1107 34-35 0.2404637080416947 1107 36-37 0.2972479537387329 1107 38-39 0.2767097795093605 1107 40-41 0.2804457811716432 1107 42-43 0.29999651509724856 1107 44-45 0.1902818269512494 1107 46-47 0.2540023719219491 1107 48-49 0.21139971121468193 1107 50-51 0.22002114819721896 1107 52-53 0.21933500006883122 1107 54-55 0.16889752211269382 1107 56-57 0.09654030783261192 1107 58-59 0.09213320208488085 1107 60-61 0.05891804198022044 1107 62-63 -0.0015439034244693062 1107 64-65 -0.11770804473778895 1107 66-67 -10.368296442131694 1107 68-69 -8.378285495733973 1107 70-71 -6.925176966519938 1107 72-73 -1.9267915541640548 1107 74-75 -0.6393902212480569 1107 76-77 -0.40079171057634255 1107 78-79 2.74981026340944 1107 80-81 0.6934172931538889 1107 82-83 0.025854573701174388 1107 84-85 -5.563203328327919 1107 86-87 -6.003939897107472 1107 88-89 -1.6025185834065105 1107 90-91 -0.41319247359199807 1107 92-93 0.0014573024164121762 1107 94-95 0.11802694839099814 1107 96-97 0.20640615063892298 1107 98-99 0.2925160013796777 1107 100 0.329916267925217 1108 1 0.011850620885414997 1108 2 0.032393672370840676 1108 3 0.035130594575534246 1108 4 0.02148253550183199 1108 5 0.0068701216120743425 1108 6 0.03105630645122659 1108 7 -12.218778393795638 1108 8 -6.738784771471124 1108 9 -2.6651848776736173 1108 10-11 -0.6276541767296351 1108 12-13 -0.044761493701166444 1108 14-15 0.1355281388453662 1108 16-17 0.1643327302098001 1108 18-19 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2216 9 -0.041131274968329024 2216 10-11 -0.7169603331706256 2216 12-13 -1.119123744467906 2216 14-15 -1.2924021444219989 2216 16-17 -1.4542827990798841 2216 18-19 -1.947130948305876 2216 20-21 -1.5312647227244227 2216 22-23 -1.3487423234707094 2216 24-25 -1.3395041975195383 2216 26-27 -1.795955422317057 2216 28-29 -1.9703143818495192 2216 30-31 -1.8604725488537284 2216 32-33 -2.7772658582353458 2216 34-35 -1.8440906027006392 2216 36-37 -1.545113017242997 2216 38-39 -1.801566873231259 2216 40-41 -1.8263784764875766 2216 42-43 -1.4542624340900616 2216 44-45 -1.6346372705815 2216 46-47 -1.8434935475768626 2216 48-49 -1.9163461336479557 2216 50-51 -2.405079722844796 2216 52-53 -2.172030077217798 2216 54-55 -1.7740600086579477 2216 56-57 -1.8437590929863177 2216 58-59 -2.106721044831417 2216 60-61 -2.291645378730607 2216 62-63 -2.205280794113733 2216 64-65 -1.8291902869900625 2216 66-67 1.5493089863489047 2216 68-69 0.6888421927214132 2216 70-71 0.32126608549728175 2216 72-73 -1.4270244784898587 2216 74-75 -2.1396533770364172 2216 76-77 -1.8382975119879283 2216 78-79 0.6612274016073201 2216 80-81 -0.7837260517608229 2216 82-83 -1.6313816787056794 2216 84-85 0.13924756713142372 2216 86-87 0.1174918008369552 2216 88-89 -1.0481800252539166 2216 90-91 -1.3147275276499855 2216 92-93 -1.38407335687468 2216 94-95 -1.339397307340132 2216 96-97 -1.3179186402305554 2216 98-99 -1.3306429970756817 2216 100 -2.4559083634565724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 110426.0 3 22359.0 4 13202.0 5 12031.0 6 11615.0 7 14088.0 8 17127.0 9 19569.0 10 21961.0 11 24623.0 12 27404.0 13 30963.0 14 34481.0 15 36372.0 16 37897.0 17 38643.0 18 36958.0 19 35159.0 20 34266.0 21 35156.0 22 37844.0 23 43580.0 24 52162.0 25 64753.0 26 81085.0 27 100372.0 28 125054.0 29 158100.0 30 194294.0 31 237700.0 32 310812.0 33 428155.0 34 630580.0 35 854618.0 36 943014.0 37 1543091.0 38 1732299.0 39 587598.0 40 1572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.981376522573235 20.750683199153627 14.442766602142779 22.825173676130362 2 15.46981192192692 20.573987347818303 40.2996183981187 23.65658233213607 3 18.26353768930456 25.421779856135686 30.571870665122198 25.74281178943756 4 12.814128675791302 17.29459700794149 38.874131158400175 31.01714315786704 5 13.06655439649827 37.738370084271274 35.522616871185996 13.672458648044463 6 30.35758802411843 38.06238195311795 18.005020421130983 13.575009601632637 7 26.756464224089214 32.77972024577237 22.696803206437938 17.767012323700477 8 24.45273870893195 36.3619411095781 21.02491940262991 18.160400778860037 9 24.394933613301845 17.566502531809068 22.179416205248312 35.859147649640775 10-11 23.375940669373225 27.42641760085793 29.14403334270299 20.05360838706585 12-13 23.89724359376972 24.973581346628862 29.10688649091298 22.02228856868844 14-15 21.41512573585831 27.413438511435157 26.292037177054343 24.87939857565219 16-17 20.88219312890829 30.601738059613513 27.057434059403924 21.458634752074275 18-19 20.82082186866168 29.579236111087276 28.924487097160583 20.67545492309046 20-21 21.987718313571634 28.84088423932231 28.39024265237018 20.78115479473588 22-23 21.673743293373672 28.739333864318677 28.39254788535201 21.194374956955635 24-25 21.443932564563966 28.809637314247148 28.44100028566581 21.305429835523075 26-27 21.19242555026819 28.82099974087529 28.47643824223069 21.510136466625823 28-29 21.16307170486432 28.86151935085562 28.64097779391631 21.334431150363752 30-31 21.52979370454065 29.215828998628012 28.154415135827122 21.099962161004214 32-33 21.15350998320326 29.04036411469558 28.535718298012743 21.27040760408842 34-35 21.33256675266579 28.654125243729805 28.5375078917062 21.475800111898202 36-37 21.626849248745735 28.90160968184124 28.138165711815905 21.33337535759712 38-39 21.40753341244728 29.009233405091123 28.33015043280621 21.253082749655388 40-41 21.281709464582498 28.712647577088728 28.530328255433528 21.47531470289524 42-43 21.216527486969994 28.745725020701347 28.50249136881479 21.535256123513868 44-45 21.340485401616846 28.844139459153723 28.36737304152126 21.448002097708173 46-47 21.250877840572542 28.945706393596687 28.319919111392238 21.483496654438532 48-49 21.091639240135603 28.707134986125045 28.47147350944888 21.72975226429047 50-51 21.09830649947809 28.63723705941278 28.4152645685571 21.84919187255204 52-53 21.030725340154248 28.655882345202087 28.463798202508855 21.84959411213481 54-55 21.11265817144257 28.878421173990176 28.35499411648653 21.65392653808073 56-57 21.018522517410354 28.628499696396716 28.310498495712228 22.0424792904807 58-59 20.941975737869136 28.313728478161465 28.56597803418783 22.178317749781574 60-61 21.11962208791826 28.5466925542529 28.251372947014765 22.082312410814072 62-63 20.954074711359894 28.512428392341754 28.29939230199619 22.234104594302163 64-65 20.954420682989312 28.258419186115454 28.40484031162296 22.382319819272272 66-67 20.84375632188962 28.253680535433645 28.26671983608014 22.63584330659659 68-69 21.32389312171668 28.30535262612251 28.083615181261933 22.28713907089887 70-71 21.008031395898723 28.059603973773672 28.184484426095924 22.74788020423168 72-73 20.78819510366317 28.055900311656213 28.415074612325995 22.740829972354618 74-75 20.826406002237796 28.025321654688273 28.341301766212013 22.80697057686191 76-77 20.857612380470606 28.02730812528449 28.163913965550275 22.951165528694634 78-79 21.02743435147766 27.969866706485107 28.23507009798471 22.767628844052528 80-81 20.778808757431165 27.841324474398306 28.305351125340568 23.07451564282996 82-83 20.748807990665625 27.761937583564787 28.448069088175142 23.041185337594445 84-85 20.836538502416253 27.596539206915754 28.321894326288945 23.245027964379048 86-87 20.99405830966864 27.646733334519357 28.21260942136806 23.14659893444394 88-89 20.95406200337336 27.45410141602982 28.273209678650137 23.318626901946686 90-91 20.979447725737202 27.280937026693998 28.226123239509736 23.51349200805907 92-93 21.099880100861718 27.31413917452647 28.10943770965188 23.476543014959937 94-95 21.10619962855074 27.043604473065063 28.24146455829807 23.608731340086127 96-97 21.160180234590527 27.019183031785886 28.19860538940759 23.622031344215998 98-99 21.290953899929853 26.94116683326604 28.075629060895512 23.69225020590859 100 21.41708140266575 26.861712716663806 27.95184486013386 23.769361020536582 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2776.0 1 2185.5 2 1927.0 3 2181.5 4 1958.5 5 1703.5 6 1563.5 7 1564.0 8 1633.5 9 1699.5 10 1594.0 11 1450.0 12 1503.5 13 1615.5 14 1759.0 15 2046.5 16 2471.0 17 3106.0 18 4212.5 19 5875.5 20 8449.0 21 12233.0 22 18138.0 23 26734.5 24 37264.0 25 50104.0 26 67356.0 27 89010.5 28 115404.0 29 142197.0 30 164598.0 31 187182.5 32 209589.5 33 229546.5 34 248221.5 35 265532.5 36 280511.0 37 293408.5 38 303950.0 39 310579.5 40 315498.5 41 316774.5 42 312404.0 43 309892.0 44 306866.5 45 300738.0 46 295292.0 47 287996.5 48 279421.5 49 274078.5 50 267512.5 51 255584.0 52 242450.5 53 231835.5 54 222904.0 55 210654.5 56 192199.0 57 170618.0 58 150671.5 59 132746.5 60 113900.0 61 94437.0 62 78198.5 63 63537.5 64 50276.5 65 39620.0 66 30378.5 67 23147.0 68 17647.5 69 13332.5 70 9977.5 71 7216.0 72 5366.0 73 3999.5 74 2952.5 75 2120.5 76 1423.0 77 958.0 78 724.0 79 571.5 80 398.5 81 231.0 82 166.5 83 131.0 84 101.5 85 86.5 86 63.0 87 45.5 88 41.5 89 36.5 90 32.5 91 25.0 92 19.5 93 20.0 94 22.0 95 20.0 96 21.5 97 22.0 98 20.5 99 26.0 100 49.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01812153164009128 2 0.00226519145501141 3 0.0012355589754607691 4 0.00203638423733349 5 0.0018647788240750496 6 0.0032261817692586753 7 11.626541316920534 8 3.432108265168803E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.260502851910363E-4 18-19 0.0 20-21 1.1440360883896011E-5 22-23 1.1440360883896011E-5 24-25 0.0 26-27 2.0592649591012817E-4 28-29 0.0 30-31 5.720180441948006E-6 32-33 1.1440360883896011E-5 34-35 2.8600902209740026E-5 36-37 1.1440360883896011E-5 38-39 4.5761443535584045E-5 40-41 5.720180441948006E-6 42-43 5.720180441948005E-5 44-45 1.7160541325844016E-4 46-47 8.408665249663569E-4 48-49 6.292198486142806E-4 50-51 4.0041263093636034E-4 52-53 2.516879394457122E-4 54-55 3.718117287266204E-4 56-57 0.0015501688997679093 58-59 4.1185299182025634E-4 60-61 0.0013042011407641452 62-63 0.00240819596606011 64-65 0.001138315907947653 66-67 5.813756873797833 68-69 5.451091713597887 70-71 0.0025569206575507585 72-73 0.0011611966297154451 74-75 0.0027800076947867305 76-77 0.005805983148577225 78-79 5.493129319665763 80-81 0.0049079148191913885 82-83 0.004398818759858016 84-85 5.813854116865345 86-87 0.003300544115003999 88-89 0.0015558890802098574 90-91 0.0031060579799777666 92-93 0.003466429347820491 94-95 0.004055607933341135 96-97 0.0030145350929065987 98-99 0.005193923841288789 100 0.005525694306921773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8740983.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.37441526823052 #Duplication Level Percentage of deduplicated Percentage of total 1 91.14143339759536 78.72288016425676 2 6.240975142559443 10.78121157284267 3 1.3637545235258448 3.533804986168475 4 0.5024583906778467 1.7359819876566058 5 0.2733637115453048 1.1805815370139467 6 0.1397172577318515 0.72407978636816 7 0.08719839214540269 0.5272197093722316 8 0.062033947209873645 0.42865167336264925 9 0.03908393534247521 0.3038266855428862 >10 0.14789363023291724 1.9246042817307758 >50 0.0018277228380508476 0.10198072049729892 >100 2.461050598892934E-4 0.027524240417567094 >500 1.3843535775000718E-5 0.007652654769987312 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.144036088389601E-5 0.0 5 0.0 0.0 0.0 3.432108265168803E-5 0.0 6 0.0 0.0 0.0 3.432108265168803E-5 1.144036088389601E-5 7 0.0 0.0 0.0 4.576144353558404E-5 1.144036088389601E-5 8 0.0 0.0 0.0 5.720180441948005E-5 1.144036088389601E-5 9 0.0 0.0 0.0 8.008252618727207E-5 1.144036088389601E-5 10-11 0.0 0.0 0.0 9.724306751311609E-5 1.144036088389601E-5 12-13 0.0 1.144036088389601E-5 0.0 1.258439697228561E-4 1.144036088389601E-5 14-15 0.0 4.0041263093636034E-5 0.0 1.5444487193259614E-4 1.144036088389601E-5 16-17 0.0 4.576144353558404E-5 0.0 1.7732559370038816E-4 1.144036088389601E-5 18-19 0.0 4.576144353558404E-5 0.0 2.0592649591012817E-4 1.144036088389601E-5 20-21 0.0 4.576144353558404E-5 0.0 3.031695634232443E-4 2.288072176779202E-5 22-23 0.0 4.576144353558404E-5 0.0 6.921418334757087E-4 2.288072176779202E-5 24-25 0.0 5.720180441948005E-5 0.0 0.0017961366587716738 2.288072176779202E-5 26-27 0.0 6.864216530337606E-5 0.0 0.003552232054449711 2.288072176779202E-5 28-29 0.0 6.864216530337606E-5 0.0 0.010588053998045757 3.432108265168803E-5 30-31 0.0 6.864216530337606E-5 0.0 0.03295395952606246 3.432108265168803E-5 32-33 0.0 6.864216530337606E-5 0.0 0.08499616118690541 3.432108265168803E-5 34-35 0.0 6.864216530337606E-5 0.0 0.17221175238528663 3.432108265168803E-5 36-37 0.0 7.436234574532407E-5 0.0 0.313763337601732 3.432108265168803E-5 38-39 0.0 8.008252618727207E-5 0.0 0.5065219781344958 3.432108265168803E-5 40-41 0.0 8.008252618727207E-5 0.0 0.7131406158780997 3.432108265168803E-5 42-43 0.0 8.008252618727207E-5 0.0 0.9309021651226184 3.432108265168803E-5 44-45 0.0 8.008252618727207E-5 0.0 1.200065255818482 3.432108265168803E-5 46-47 0.0 8.580270662922007E-5 0.0 1.5262013437161472 3.432108265168803E-5 48-49 0.0 1.0296324795506408E-4 0.0 1.8741828007216124 3.432108265168803E-5 50-51 0.0 1.144036088389601E-4 0.0 2.2270893330875943 3.432108265168803E-5 52-53 0.0 1.144036088389601E-4 0.0 2.573377616682243 3.432108265168803E-5 54-55 0.0 1.144036088389601E-4 0.0 2.9495652834469532 3.432108265168803E-5 56-57 0.0 1.144036088389601E-4 0.0 3.3527121606345647 3.432108265168803E-5 58-59 0.0 1.144036088389601E-4 0.0 3.7901629599325384 3.432108265168803E-5 60-61 0.0 1.144036088389601E-4 0.0 4.2369662542530975 3.432108265168803E-5 62-63 0.0 1.144036088389601E-4 0.0 4.672672398516276 3.432108265168803E-5 64-65 0.0 1.144036088389601E-4 0.0 5.134199437294409 3.432108265168803E-5 66-67 0.0 1.144036088389601E-4 0.0 5.642134299998066 3.432108265168803E-5 68-69 0.0 1.144036088389601E-4 0.0 6.136049000438509 3.432108265168803E-5 70-71 0.0 1.2012378928090812E-4 0.0 6.709805979487662 3.432108265168803E-5 72-73 0.0 1.2584396972285613E-4 0.0 7.313016167632405 3.432108265168803E-5 74-75 0.0 1.2584396972285613E-4 0.0 7.8440719996824155 3.432108265168803E-5 76-77 0.0 1.3156415016480412E-4 0.0 8.386516710992346 3.432108265168803E-5 78-79 0.0 1.3728433060675212E-4 0.0 8.999611370940773 3.432108265168803E-5 80-81 0.0 1.3728433060675212E-4 0.0 9.706923122948528 3.432108265168803E-5 82-83 0.0 1.4872469149064814E-4 0.0 10.415819364938702 3.432108265168803E-5 84-85 0.0 1.4872469149064814E-4 0.0 11.099695537675796 3.432108265168803E-5 86-87 0.0 1.4872469149064814E-4 0.0 11.879642140935408 3.432108265168803E-5 88 0.0 1.4872469149064814E-4 0.0 12.511212983711328 3.432108265168803E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8640 0.0 46.09652 1 GTATCAA 13945 0.0 37.297302 1 ATCAACG 14705 0.0 33.65774 3 TCAACGC 14790 0.0 33.464306 4 CAACGCA 16290 0.0 30.414299 5 TATCAAC 17130 0.0 29.550337 2 AACGCAG 17730 0.0 28.00075 6 ACGCAGA 20145 0.0 24.186527 7 GTACATG 23805 0.0 23.89176 1 CGCAGAG 20435 0.0 23.483604 8 TACATGG 23755 0.0 23.011227 2 GTGGTAT 4960 0.0 22.396507 1 ACATGGG 24410 0.0 21.534073 3 TAGACCG 430 0.0 21.425333 5 GCAGAGT 23425 0.0 19.559326 9 CATGGGG 16860 0.0 18.001995 4 TGGTATC 6080 0.0 17.425705 2 CAGAGTA 22575 0.0 17.030119 10-11 GAGTACA 18440 0.0 17.017736 1 AGAGTAC 20330 0.0 16.875036 10-11 >>END_MODULE