FastQCFastQC Report
Wed 25 May 2016
SRR2049532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8740983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268650.30734529514586634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263040.30092725269000065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147620.16888260736807292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169900.059.0187151
GTATCAA299600.040.115531
GTGGTAT65000.033.1707761
ATCAACG379450.031.3476223
TCAACGC385250.030.8390394
TATCAAC393900.030.2575552
CAACGCA400150.029.7270495
AACGCAG418550.028.5209436
TGGTATC74950.027.9993422
ACGCAGA471300.025.1090817
CGCAGAG470250.025.0452168
GCAGAGT504450.022.7882469
GTACATG237700.022.240991
GAGTACT370050.021.59041812-13
TACATGG239200.021.0871662
CAGAGTA494100.021.0826610-11
AGAGTAC470200.020.3151710-11
ACATGGG243900.019.9090273
GTACTTT402000.019.58803714-15
AGTACTT385700.018.92928112-13
Produced by FastQC (version 0.11.5)