##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049532_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8740983
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793330452650462	31.0	31.0	34.0	30.0	34.0
2	31.887489542080107	33.0	31.0	34.0	30.0	34.0
3	31.910523335876526	33.0	31.0	34.0	30.0	34.0
4	35.574669119022424	37.0	35.0	37.0	33.0	37.0
5	35.70706280975492	37.0	35.0	37.0	33.0	37.0
6	35.77724244515748	37.0	35.0	37.0	33.0	37.0
7	35.763180296769825	37.0	35.0	37.0	33.0	37.0
8	35.74414159139767	37.0	35.0	37.0	33.0	37.0
9	37.45489048542939	39.0	37.0	39.0	34.0	39.0
10-11	37.42881561490281	39.0	37.0	39.0	34.0	39.0
12-13	37.41906585334853	39.0	37.0	39.0	34.0	39.0
14-15	38.672678004293104	40.0	38.0	41.0	34.0	41.0
16-17	38.67882479579242	40.0	38.0	41.0	34.0	41.0
18-19	38.76106966459035	40.0	38.0	41.0	34.5	41.0
20-21	38.79810571648521	40.0	38.0	41.0	34.0	41.0
22-23	38.772649597877034	40.0	38.0	41.0	34.0	41.0
24-25	38.73730609017315	40.0	38.0	41.0	34.0	41.0
26-27	38.64308064665038	40.0	38.0	41.0	34.0	41.0
28-29	38.55542231348579	40.0	38.0	41.0	34.0	41.0
30-31	38.4332472674984	40.0	38.0	41.0	34.0	41.0
32-33	38.28211260678576	40.0	38.0	41.0	34.0	41.0
34-35	38.1786446101085	40.0	38.0	41.0	34.0	41.0
36-37	38.038182719266246	40.0	37.5	41.0	33.0	41.0
38-39	37.94308082969616	40.0	37.0	41.0	33.0	41.0
40-41	37.81506433544145	40.0	37.0	41.0	33.0	41.0
42-43	37.67033587641115	40.0	37.0	41.0	33.0	41.0
44-45	37.478201765179044	40.0	37.0	41.0	33.0	41.0
46-47	37.225010505111385	40.0	37.0	41.0	32.0	41.0
48-49	36.98679284698299	40.0	37.0	41.0	31.0	41.0
50-51	36.770961458225	40.0	36.0	41.0	31.0	41.0
52-53	36.66914922497847	40.0	36.0	41.0	31.0	41.0
54-55	36.543992020119475	39.5	36.0	41.0	31.0	41.0
56-57	36.37482157327156	39.0	35.0	41.0	30.0	41.0
58-59	36.20495440844582	39.0	35.0	41.0	30.0	41.0
60-61	36.022110556673084	39.0	35.0	41.0	30.0	41.0
62-63	35.828762851958416	39.0	35.0	41.0	29.5	41.0
64-65	35.55671255738628	38.0	35.0	40.0	29.0	41.0
66-67	35.30691044702867	37.5	35.0	40.0	29.0	41.0
68-69	34.999667199901886	37.0	34.5	40.0	28.5	41.0
70-71	34.71058878618115	37.0	34.0	39.5	28.0	41.0
72-73	34.39393652864901	36.0	34.0	39.0	28.0	41.0
74-75	34.05240228701966	36.0	34.0	39.0	28.0	40.5
76-77	33.759584648545825	35.0	34.0	38.5	28.0	40.0
78-79	33.42730943419063	35.0	34.0	37.0	27.0	39.0
80-81	33.11400045052141	35.0	34.0	37.0	27.0	39.0
82-83	32.81382980609847	35.0	33.0	36.0	26.5	39.0
84-85	32.535000411280976	35.0	33.0	36.0	26.0	37.0
86-87	32.25355574996542	35.0	33.0	36.0	26.0	37.0
88-89	32.03282880197799	35.0	33.0	35.0	26.0	37.0
90-91	31.87316460860295	35.0	33.0	35.0	26.0	36.0
92-93	31.7073938937989	35.0	33.0	35.0	25.0	36.0
94-95	31.518830490804067	35.0	33.0	35.0	25.0	36.0
96-97	31.343885350194597	35.0	33.0	35.0	25.0	36.0
98-99	31.15451768982962	35.0	33.0	35.0	24.0	35.0
100	31.032454015755437	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34863874390902083
1101	2	0.396721823462137
1101	3	0.4671658741783382
1101	4	0.34601737464574
1101	5	0.278038120041991
1101	6	0.2723183441840078
1101	7	0.2938288780333451
1101	8	0.28658994070935506
1101	9	0.3370703067282079
1101	10-11	0.37333962485049454
1101	12-13	0.2934081185210289
1101	14-15	0.4480049574419027
1101	16-17	0.4517629663294258
1101	18-19	0.4051795835187235
1101	20-21	0.4134145812289347
1101	22-23	0.4625088127915902
1101	24-25	0.48529054576773945
1101	26-27	0.46927098805790024
1101	28-29	0.43848099315324873
1101	30-31	0.4439199321430394
1101	32-33	0.4788457686084371
1101	34-35	0.5047740512491217
1101	36-37	0.48535572373248215
1101	38-39	0.5288855468912672
1101	40-41	0.5484391992111526
1101	42-43	0.4530942607942734
1101	44-45	0.473277902490544
1101	46-47	0.5436221899802547
1101	48-49	0.5490443219100882
1101	50-51	0.5822826077176657
1101	52-53	0.5368964471618654
1101	54-55	0.5021105282632945
1101	56-57	0.5412911802250022
1101	58-59	0.5492193450915082
1101	60-61	0.5703411377719831
1101	62-63	0.5704210011463715
1101	64-65	0.5591579164212988
1101	66-67	0.549680320038938
1101	68-69	0.5028716052256783
1101	70-71	0.5267951166071327
1101	72-73	0.5018532837399547
1101	74-75	0.4797340712361162
1101	76-77	0.48765538934515007
1101	78-79	0.500867814220527
1101	80-81	0.4997535247405125
1101	82-83	0.4876814937560141
1101	84-85	0.4730422777351819
1101	86-87	0.5453708219666353
1101	88-89	0.5520048507615201
1101	90-91	0.5514240667990151
1101	92-93	0.5471558811685711
1101	94-95	0.48524518910037173
1101	96-97	0.527859041823934
1101	98-99	0.5667482477272934
1101	100	0.6477902623214113
1102	1	0.30558114669587866
1102	2	0.29973515104842363
1102	3	0.4026919261726114
1102	4	0.37567765135000286
1102	5	0.2883758162686476
1102	6	0.26100716239671584
1102	7	0.26512056125304184
1102	8	0.2709285603649505
1102	9	0.3953216573268392
1102	10-11	0.32746484305236123
1102	12-13	0.28051797050041216
1102	14-15	0.3954447923971216
1102	16-17	0.41990675542130873
1102	18-19	0.40798667143813105
1102	20-21	0.3859256985203672
1102	22-23	0.39069592121425956
1102	24-25	0.36616006532744194
1102	26-27	0.351062386293151
1102	28-29	0.3706756950051897
1102	30-31	0.41802054517916787
1102	32-33	0.45728048816125266
1102	34-35	0.5149286843992797
1102	36-37	0.57715786427962
1102	38-39	0.5345703779003443
1102	40-41	0.4919690551402951
1102	42-43	0.522328361450036
1102	44-45	0.5285323791690999
1102	46-47	0.5556991435297078
1102	48-49	0.5989859386858001
1102	50-51	0.6579946019190785
1102	52-53	0.610021665818067
1102	54-55	0.6187070157877486
1102	56-57	0.6543777426197508
1102	58-59	0.6009272398195193
1102	60-61	0.5828997842340584
1102	62-63	0.5906895952903426
1102	64-65	0.5942765506148575
1102	66-67	0.6091739073945917
1102	68-69	0.5939154244841518
1102	70-71	0.6047115177387639
1102	72-73	0.625780684164944
1102	74-75	0.5675714148315834
1102	76-77	0.5406311324084712
1102	78-79	0.5271361792079432
1102	80-81	0.493515596610429
1102	82-83	0.5108757510108575
1102	84-85	0.5286254347999986
1102	86-87	0.5808375961886227
1102	88-89	0.5385373079281806
1102	90-91	0.5045493191005406
1102	92-93	0.557416254557701
1102	94-95	0.5792571548248269
1102	96-97	0.5815878015124518
1102	98-99	0.6372466909743402
1102	100	0.7252925804409251
1103	1	0.2757226602604348
1103	2	0.3191016821377133
1103	3	0.42968683002972696
1103	4	0.34880331939092457
1103	5	0.26401611245307777
1103	6	0.2942718049516415
1103	7	0.3092947603630094
1103	8	0.2721620020809823
1103	9	0.33337234416861605
1103	10-11	0.2783643578164785
1103	12-13	0.3096196922583161
1103	14-15	0.40553036764168127
1103	16-17	0.37411638682773685
1103	18-19	0.3627640688717051
1103	20-21	0.37068787485192445
1103	22-23	0.36392400936199465
1103	24-25	0.39487575899208593
1103	26-27	0.39278884385997515
1103	28-29	0.39811185240520075
1103	30-31	0.3918089186158582
1103	32-33	0.38823338459307166
1103	34-35	0.4051108975800801
1103	36-37	0.4202556511692279
1103	38-39	0.40358221198813027
1103	40-41	0.4059625680201506
1103	42-43	0.3737618909107283
1103	44-45	0.41839567087872354
1103	46-47	0.4054167236605224
1103	48-49	0.3979466001238734
1103	50-51	0.36331479291552427
1103	52-53	0.3491444524546523
1103	54-55	0.3554195745830526
1103	56-57	0.43328430511933647
1103	58-59	0.35842965015737605
1103	60-61	0.3328229966566596
1103	62-63	0.35316592645470735
1103	64-65	0.40999824603176904
1103	66-67	0.41191410179698096
1103	68-69	0.39278972740036977
1103	70-71	0.3915142194293608
1103	72-73	0.43370877050850254
1103	74-75	0.38290488193811
1103	76-77	0.35432444766482263
1103	78-79	0.33508010711474867
1103	80-81	0.30160693906410074
1103	82-83	0.2952674547841809
1103	84-85	0.36142290525437915
1103	86-87	0.3963623721735203
1103	88-89	0.4248044547702321
1103	90-91	0.3481323638754006
1103	92-93	0.3570617335735662
1103	94-95	0.36435589836883864
1103	96-97	0.3476228738533891
1103	98-99	0.41449847017991104
1103	100	0.41939463443161173
1104	1	0.22897545392385865
1104	2	0.25589285630280045
1104	3	0.3100532432880918
1104	4	0.2679247859298215
1104	5	0.19024795782499382
1104	6	0.19843527970543562
1104	7	0.16183239944349737
1104	8	0.17594579232414276
1104	9	0.20923065412650033
1104	10-11	0.23917226421438187
1104	12-13	0.2520923040944183
1104	14-15	0.31138344827217423
1104	16-17	0.3160657487231404
1104	18-19	0.29871675240966766
1104	20-21	0.2935782266206246
1104	22-23	0.2995464976013693
1104	24-25	0.3280219756966858
1104	26-27	0.34481989242617317
1104	28-29	0.3006601194684322
1104	30-31	0.3619039736602545
1104	32-33	0.35534094298509444
1104	34-35	0.3587425164262399
1104	36-37	0.37168366494920235
1104	38-39	0.4070241027715511
1104	40-41	0.45627994722188703
1104	42-43	0.43714637720717064
1104	44-45	0.4574031474004485
1104	46-47	0.48441326560975995
1104	48-49	0.4706705329545571
1104	50-51	0.5215879023589949
1104	52-53	0.4960851782778235
1104	54-55	0.4391548715117466
1104	56-57	0.44936031708866153
1104	58-59	0.4479252237652034
1104	60-61	0.40885673753749074
1104	62-63	0.4107411140113797
1104	64-65	0.4521915163248096
1104	66-67	0.4682335979212482
1104	68-69	0.4116868897520476
1104	70-71	0.42068841847982696
1104	72-73	0.43865822807190114
1104	74-75	0.46128352099850645
1104	76-77	0.40443851219552585
1104	78-79	0.39365603678467664
1104	80-81	0.38795070560688316
1104	82-83	0.4025019551678355
1104	84-85	0.45485016923905164
1104	86-87	0.508540360963778
1104	88-89	0.42755488650463747
1104	90-91	0.4256441833249127
1104	92-93	0.45375402828145894
1104	94-95	0.4455475443822259
1104	96-97	0.48523447084425086
1104	98-99	0.5221956234700009
1104	100	0.5724154741594418
1105	1	0.22808310133945753
1105	2	0.25023977360518046
1105	3	0.34523232489332756
1105	4	0.2403961966529451
1105	5	0.20589456482355928
1105	6	0.20937860158532118
1105	7	0.19641219093306717
1105	8	0.18949252539021444
1105	9	0.22327166960610612
1105	10-11	0.21290930794236118
1105	12-13	0.24098418779679776
1105	14-15	0.30127170627327615
1105	16-17	0.2733132667117104
1105	18-19	0.3271025796594884
1105	20-21	0.30373438288875576
1105	22-23	0.29556437293298643
1105	24-25	0.28673594887844445
1105	26-27	0.30072999637662434
1105	28-29	0.34464081176543715
1105	30-31	0.3575649886298322
1105	32-33	0.37856688312335507
1105	34-35	0.310055749113225
1105	36-37	0.3957401205497888
1105	38-39	0.38776431541953826
1105	40-41	0.40508648516366463
1105	42-43	0.41686395802869924
1105	44-45	0.45087175684255953
1105	46-47	0.44797345852992976
1105	48-49	0.46324446489782645
1105	50-51	0.48490159272359534
1105	52-53	0.47458344511292694
1105	54-55	0.46567634146362025
1105	56-57	0.46614298372765006
1105	58-59	0.4597712230905131
1105	60-61	0.459399139517636
1105	62-63	0.44730162616750846
1105	64-65	0.42916542919369505
1105	66-67	0.43001555061543684
1105	68-69	0.4192146994262771
1105	70-71	0.4338317312707005
1105	72-73	0.44105084514753656
1105	74-75	0.4279331162572291
1105	76-77	0.4320664565512118
1105	78-79	0.3881002307483783
1105	80-81	0.3755015282366827
1105	82-83	0.3881712889614164
1105	84-85	0.430219779095772
1105	86-87	0.4533106681559005
1105	88-89	0.413646968175442
1105	90-91	0.4417015494852379
1105	92-93	0.4168626948050971
1105	94-95	0.474449127291539
1105	96-97	0.521616440775297
1105	98-99	0.5337584594201381
1105	100	0.5208451854859639
1106	1	0.17840399192538925
1106	2	0.16916218114662485
1106	3	0.26298370873153587
1106	4	0.21856843710421714
1106	5	0.18686080880144829
1106	6	0.20177689633651852
1106	7	0.18226925199897437
1106	8	0.1697307592678925
1106	9	0.1729793123947161
1106	10-11	0.2122470014091462
1106	12-13	0.1732120272929336
1106	14-15	0.25231683195524823
1106	16-17	0.22011156164566614
1106	18-19	0.22483992483373783
1106	20-21	0.22090018416200508
1106	22-23	0.19113591008901665
1106	24-25	0.20494954227032736
1106	26-27	0.21865120644757496
1106	28-29	0.2376278744453444
1106	30-31	0.23442057724828658
1106	32-33	0.2310619454831624
1106	34-35	0.21669538094784002
1106	36-37	0.22723828328680895
1106	38-39	0.23414455094237496
1106	40-41	0.26184617083440287
1106	42-43	0.2627782851464957
1106	44-45	0.2617569117724301
1106	46-47	0.21533276086042008
1106	48-49	0.2006036186215283
1106	50-51	0.2512732636648991
1106	52-53	0.20978901334635225
1106	54-55	0.17280965681327842
1106	56-57	0.1819936431697542
1106	58-59	0.19987117671562515
1106	60-61	0.22261038674987077
1106	62-63	0.24889019688217218
1106	64-65	0.2514980776012905
1106	66-67	0.2203786622841477
1106	68-69	0.17881735815193167
1106	70-71	0.20359720168387696
1106	72-73	0.21865460586979424
1106	74-75	0.2057736361033804
1106	76-77	0.1864090222755621
1106	78-79	0.20025618589689032
1106	80-81	0.12893885448586673
1106	82-83	0.13212787171362095
1106	84-85	0.18579868044727732
1106	86-87	0.19689598958195376
1106	88-89	0.2167185117500594
1106	90-91	0.2109471680978956
1106	92-93	0.24538019800906596
1106	94-95	0.22100987546386008
1106	96-97	0.24134887609424638
1106	98-99	0.314175996205325
1106	100	0.32340444902638055
1107	1	0.1557898901177417
1107	2	0.14667539619875214
1107	3	0.230750305482875
1107	4	0.1796952994043508
1107	5	0.14462251133034698
1107	6	0.15498298373154995
1107	7	0.1432564182245244
1107	8	0.18058131727995175
1107	9	0.13911483277535552
1107	10-11	0.13447502231620234
1107	12-13	0.13470225532875446
1107	14-15	0.199680672288018
1107	16-17	0.16720773216600548
1107	18-19	0.133999206535087
1107	20-21	0.17919413418231755
1107	22-23	0.18077896659170278
1107	24-25	0.18759320487389886
1107	26-27	0.19781481969920378
1107	28-29	0.22844325006671795
1107	30-31	0.2245312882298478
1107	32-33	0.22696231055571303
1107	34-35	0.19476095036961283
1107	36-37	0.24241576138718557
1107	38-39	0.18991568320731034
1107	40-41	0.17004468554652163
1107	42-43	0.13968031839034012
1107	44-45	0.20752291483414353
1107	46-47	0.21959579821900377
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2216	66-67	-1.3396068440202953
2216	68-69	-1.6238310878540432
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2216	72-73	-1.2661634974936433
2216	74-75	-1.3339118205003757
2216	76-77	-1.411406790143161
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2216	80-81	-1.2338181022407682
2216	82-83	-1.3302356668863382
2216	84-85	-1.3454111426575608
2216	86-87	-1.523776177154705
2216	88-89	-1.371114726646951
2216	90-91	-1.2326881490770916
2216	92-93	-1.3879623802374468
2216	94-95	-1.4840588608962335
2216	96-97	-1.5716078689823796
2216	98-99	-1.582798055063673
2216	100	-1.5525463600765121
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	533.0
3	62.0
4	87.0
5	173.0
6	528.0
7	1478.0
8	3048.0
9	5229.0
10	7599.0
11	10953.0
12	15707.0
13	22065.0
14	26733.0
15	34337.0
16	43601.0
17	60559.0
18	61048.0
19	33582.0
20	24144.0
21	23082.0
22	24498.0
23	26535.0
24	31291.0
25	38298.0
26	49029.0
27	63519.0
28	82392.0
29	108568.0
30	141912.0
31	182671.0
32	235302.0
33	304933.0
34	414176.0
35	592647.0
36	960883.0
37	1743486.0
38	2406161.0
39	956841.0
40	3293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37346168692109	20.86837850571918	14.948772119725252	22.809387687634477
2	15.67777161522614	20.919925573460514	39.73706344651988	23.665239364793464
3	17.915279522433412	25.572395772556394	31.150923580651142	25.361401124359052
4	12.691234373439405	17.865145863170753	38.17931956482497	31.26430019856487
5	12.519992348564967	37.59325758473667	35.60206542983721	14.284684636861156
6	29.88491295252116	38.52090331594298	17.475621969549458	14.118561761986403
7	25.9632583657925	33.42406683550351	22.556479059620642	18.05619573908335
8	24.685690385166062	36.10139729135728	20.49295828627055	18.71995403720611
9	24.502978957274575	16.888716571532385	22.149615294764498	36.45868917642854
10-11	23.462155228994266	27.28580984541441	28.74314593678995	20.508888988801374
12-13	24.427522747591073	24.819469623573696	29.173832386423925	21.579175242411306
14-15	21.609294012961016	26.886635849381683	27.14471325990604	24.359356877751264
16-17	20.2932610668617	30.117653815366076	27.933608840104142	21.655476277668086
18-19	20.188089829256047	28.19281881683101	31.12607014565753	20.49302120825541
20-21	21.263003257185147	27.445597365879788	30.956546878079962	20.334852498855106
22-23	20.94817024584077	27.333682035533073	31.049333925028797	20.66881379359736
24-25	20.789709807237926	27.387663378363737	31.080102775626035	20.7425240387723
26-27	20.574791187673057	27.431617244879664	31.091554576870816	20.902036990576462
28-29	20.590141462528425	27.42040587705807	31.25122698024888	20.738225680164632
30-31	20.974990440120546	27.724554203586965	30.789779037365182	20.510676318927302
32-33	20.570345593932764	27.59066173464969	31.127461551512354	20.711531119905192
34-35	20.78904054612622	27.265823534950247	31.0710706107082	20.874065308215332
36-37	21.08226843594136	27.509114249507178	30.582755966920427	20.82586134763104
38-39	20.915702501652277	27.539105155564307	30.749693712938235	20.79549862984518
40-41	20.82542089373701	27.330718982064145	30.82851779942828	21.015342324770568
42-43	20.825398013015242	27.389093423474225	30.637629657900035	21.1478789056105
44-45	20.97253821452347	27.546140977507903	30.38687410786636	21.094446700102264
46-47	21.025821695340216	27.739620360776357	29.998771305241068	21.23578663864236
48-49	21.030912655933548	27.666224725525723	29.753032353454984	21.549830265085745
50-51	21.082171192873844	27.721372985166543	29.48674651352142	21.709709308438192
52-53	20.988926531489653	27.818484488529492	29.474396643947255	21.7181923360336
54-55	21.051877117253287	28.05038632382651	29.338736844586016	21.55899971433419
56-57	20.854977066080554	27.779695944952643	29.35026300817654	22.015063980790263
58-59	20.74835634651282	27.462016656161538	29.64531565977893	22.14431133754671
60-61	20.924860510539833	27.6823670747329	29.34472015332829	22.048052261398976
62-63	20.71205263755804	27.648503606516567	29.410914081402517	22.228529674522875
64-65	20.64600171399487	27.47253369558092	29.50433034819997	22.377134242224244
66-67	20.601967764952754	27.52764763413909	29.312669982312055	22.5577146185961
68-69	20.981241009305364	27.68134060909861	29.021161466755107	22.316256914840917
70-71	20.884435995356586	27.4944305463127	28.95392886589529	22.66720459243543
72-73	20.7249058830111	27.602181585297668	28.996978943901393	22.67593358778984
74-75	20.81345427625245	27.62145859338704	28.804614995819122	22.76047213454139
76-77	20.822211872509076	27.693876077782097	28.59724701443762	22.886665035271207
78-79	20.907785197437832	27.79150909684873	28.56188729244072	22.73881841327272
80-81	20.771341170080117	27.714425319790497	28.53360335716101	22.980630152968377
82-83	20.63039822554777	27.666371771218028	28.633840871548188	23.069389131686012
84-85	20.675208955331453	27.576229126632555	28.40170264602963	23.346859272006363
86-87	20.7701687030323	27.731303843355796	28.28084742433372	23.217680029278178
88-89	20.663103258543252	27.630219575581744	28.29849731320065	23.408179852674355
90-91	20.55291635827728	27.504689549345073	28.22113107817391	23.721263014203732
92-93	20.61272237278791	27.548847950808863	28.118318605772192	23.72011107063103
94-95	20.516735485601796	27.36088512295456	28.245024200793225	23.877355190650427
96-97	20.486015159051057	27.41485296418337	28.17461881430377	23.9245130624618
98-99	20.50075269007262	27.469718360575413	28.02397533961146	24.005553609740506
100	20.586197539010964	27.43520214678712	27.886186693416082	24.092413620785834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1134.0
1	882.0
2	1201.0
3	1831.5
4	1264.5
5	774.0
6	1766.5
7	4559.0
8	7754.0
9	9050.5
10	7379.0
11	5263.5
12	5089.5
13	5761.5
14	6722.0
15	7648.5
16	8658.5
17	9833.5
18	11054.5
19	12672.0
20	15163.5
21	18494.5
22	23487.0
23	31133.0
24	40441.5
25	51507.0
26	66931.0
27	86673.5
28	111510.0
29	137484.0
30	159435.5
31	181034.0
32	202004.5
33	220774.5
34	239470.0
35	256582.5
36	270476.5
37	284025.0
38	296236.0
39	303540.0
40	309142.5
41	311590.5
42	308683.0
43	308261.0
44	307022.0
45	302393.5
46	296813.0
47	288102.5
48	278703.0
49	274060.0
50	268225.5
51	255990.5
52	241347.0
53	230616.0
54	222901.5
55	210934.0
56	193769.5
57	171648.5
58	151220.0
59	135047.0
60	116598.0
61	96451.0
62	80061.0
63	64967.5
64	51163.5
65	40228.0
66	30809.0
67	23438.5
68	17834.5
69	13446.0
70	9802.0
71	6938.5
72	5085.5
73	3756.0
74	2710.5
75	1855.0
76	1146.5
77	712.5
78	507.0
79	381.0
80	253.5
81	99.0
82	49.5
83	31.0
84	16.0
85	7.0
86	6.0
87	5.5
88	4.0
89	2.0
90	0.5
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05851744592112809
2	1.7160541325844016E-4
3	3.432108265168803E-5
4	0.11823612973506525
5	0.0015902101628615453
6	0.0013842836669514173
7	0.0
8	0.0
9	5.720180441948005E-5
10-11	0.0
12-13	2.8600902209740026E-5
14-15	1.1440360883896011E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.258439697228561E-4
30-31	2.9172920253934826E-4
32-33	2.2880721767792022E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.5444487193259614E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.1440360883896011E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.8876595458428418E-4
80-81	2.974493829812963E-4
82-83	3.6609154828467236E-4
84-85	0.0
86-87	2.2880721767792022E-5
88-89	7.436234574532407E-5
90-91	5.148162397753204E-5
92-93	6.692611117079166E-4
94-95	5.777382246367485E-4
96-97	5.720180441948006E-6
98-99	1.7160541325844015E-5
100	0.001224118614576873
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8740983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.621637704972606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01738029877482	41.90985291148405
2	15.243008944297992	17.26168259955396
3	4.790742227167058	8.137790121737003
4	2.0975883587723585	4.750755524183063
5	1.0828784450041136	3.065717549577351
6	0.6339376385841999	2.1536752379676263
7	0.4384690675874477	1.737878568284153
8	0.2823251795670133	1.2788571225947811
9	0.22414390764207281	1.142225561905766
>10	1.0945287507102088	11.096217951293754
>50	0.06157914820717794	2.3744995399367816
>100	0.03131881196235626	3.1173441895909657
>500	0.0014132738009468657	0.5205753584306152
>1k	6.052482147198807E-4	0.5835290387423686
>5k	2.0174926863780235E-5	0.093397505791728
>10k+	6.052478059134071E-5	0.7760012189258653
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26865	0.30734529514586634	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26304	0.30092725269000065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14762	0.16888260736807292	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.288072176779202E-5	0.0
6	0.0	0.0	0.0	2.288072176779202E-5	0.0
7	0.0	0.0	0.0	3.432108265168803E-5	0.0
8	0.0	0.0	0.0	4.576144353558404E-5	0.0
9	0.0	0.0	0.0	6.864216530337606E-5	0.0
10-11	0.0	0.0	0.0	8.008252618727207E-5	0.0
12-13	0.0	1.7160541325844015E-5	0.0	1.144036088389601E-4	0.0
14-15	0.0	4.5761443535584045E-5	0.0	1.6588523281649216E-4	0.0
16-17	0.0	5.720180441948005E-5	0.0	1.8304577414233615E-4	0.0
18-19	0.0	5.720180441948005E-5	0.0	2.0592649591012817E-4	0.0
20-21	0.0	5.720180441948005E-5	0.0	2.9172920253934826E-4	0.0
22-23	0.0	5.720180441948005E-5	0.0	6.521005703820726E-4	0.0
24-25	0.0	5.720180441948005E-5	0.0	0.0017274944934682975	0.0
26-27	0.0	5.720180441948005E-5	0.0	0.003466429347820491	0.0
28-29	0.0	5.720180441948005E-5	0.0	0.010530852193626278	0.0
30-31	0.0	5.720180441948005E-5	0.0	0.033154165841530636	0.0
32-33	0.0	5.720180441948005E-5	0.0	0.08574550482480059	0.0
34-35	0.0	5.720180441948005E-5	0.0	0.17407653120936167	0.0
36-37	0.0	5.720180441948005E-5	0.0	0.3175901383173952	0.0
38-39	0.0	5.720180441948005E-5	0.0	0.5122421585764438	0.0
40-41	0.0	5.720180441948005E-5	0.0	0.7208342585725198	0.0
42-43	0.0	5.720180441948005E-5	0.0	0.9404319857389037	0.0
44-45	0.0	5.720180441948005E-5	0.0	1.211597139589449	0.0
46-47	0.0	5.720180441948005E-5	0.0	1.5402100656184778	0.0
48-49	0.0	5.720180441948005E-5	0.0	1.8904109526354187	0.0
50-51	0.0	5.720180441948005E-5	0.0	2.2451536629232662	0.0
52-53	0.0	5.720180441948005E-5	0.0	2.5942848761975625	0.0
54-55	0.0	5.720180441948005E-5	0.0	2.9868723002893383	0.0
56-57	0.0	5.720180441948005E-5	0.0	3.449194444148902	0.0
58-59	0.0	5.720180441948005E-5	0.0	3.952478800153255	0.0
60-61	0.0	5.720180441948005E-5	0.0	4.465527504172014	0.0
62-63	0.0	5.720180441948005E-5	0.0	4.9670500446002475	0.0
64-65	0.0	5.720180441948005E-5	0.0	5.498792298303291	0.0
66-67	0.0	5.720180441948005E-5	0.0	6.103832944189458	0.0
68-69	0.0	5.720180441948005E-5	0.0	6.758627719559688	0.0
70-71	0.0	5.720180441948005E-5	0.0	7.436783711854834	0.0
72-73	0.0	5.720180441948005E-5	0.0	8.128776820639052	0.0
74-75	0.0	5.720180441948005E-5	0.0	8.81154899855085	0.0
76-77	0.0	6.292198486142805E-5	0.0	9.531508069515752	0.0
78-79	0.0	6.864216530337606E-5	0.0	10.317489463141618	0.0
80-81	0.0	6.864216530337606E-5	0.0	11.131276653895792	0.0
82-83	0.0	6.864216530337606E-5	0.0	11.945990513881561	0.0
84-85	0.0	6.864216530337606E-5	0.0	12.733436273700566	0.0
86-87	5.720180441948005E-6	6.864216530337606E-5	0.0	13.546691487673641	0.0
88	1.144036088389601E-5	6.864216530337606E-5	0.0	14.186825440571157	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16990	0.0	59.018715	1
GTATCAA	29960	0.0	40.11553	1
GTGGTAT	6500	0.0	33.170776	1
ATCAACG	37945	0.0	31.347622	3
TCAACGC	38525	0.0	30.839039	4
TATCAAC	39390	0.0	30.257555	2
CAACGCA	40015	0.0	29.727049	5
AACGCAG	41855	0.0	28.520943	6
TGGTATC	7495	0.0	27.999342	2
ACGCAGA	47130	0.0	25.109081	7
CGCAGAG	47025	0.0	25.045216	8
GCAGAGT	50445	0.0	22.788246	9
GTACATG	23770	0.0	22.24099	1
GAGTACT	37005	0.0	21.590418	12-13
TACATGG	23920	0.0	21.087166	2
CAGAGTA	49410	0.0	21.08266	10-11
AGAGTAC	47020	0.0	20.31517	10-11
ACATGGG	24390	0.0	19.909027	3
GTACTTT	40200	0.0	19.588037	14-15
AGTACTT	38570	0.0	18.929281	12-13
>>END_MODULE