FastQCFastQC Report
Wed 25 May 2016
SRR2049531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794756
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79500.043.4745331
GTATCAA127350.037.271691
TCAACGC132100.034.4131624
ATCAACG133950.034.0525363
CAACGCA149750.030.5280515
TATCAAC154450.029.8973772
AACGCAG164600.027.7423936
GTACATG245700.024.486741
ACGCAGA191850.023.721567
CGCAGAG193300.023.4703838
TACATGG253600.022.8715252
ACATGGG259050.021.955583
GCAGAGT226900.019.0977469
CATGGGG188500.018.739254
GAGTACA189000.018.3681831
GTGGTAT50600.018.3158191
GTCGTAC4251.8189894E-1118.0717681
CAGAGTA214450.016.64186710-11
GTATAGG28450.016.3778691
AGAGTAC196500.016.15941610-11
Produced by FastQC (version 0.11.5)