##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049531_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8794756 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.798955764093968 31.0 31.0 34.0 28.0 34.0 2 31.048207249865715 31.0 31.0 34.0 28.0 34.0 3 31.144034012995927 31.0 31.0 34.0 28.0 34.0 4 34.73466301964489 37.0 35.0 37.0 32.0 37.0 5 34.70773504119955 37.0 35.0 37.0 32.0 37.0 6 34.76924055653164 37.0 35.0 37.0 32.0 37.0 7 30.815003622613293 37.0 33.0 37.0 2.0 37.0 8 32.6139617744938 37.0 33.0 37.0 17.0 37.0 9 35.406787408314685 39.0 34.0 39.0 29.0 39.0 10-11 35.953434467084705 39.0 36.0 39.0 31.0 39.0 12-13 36.10160497914894 39.0 37.0 39.0 31.0 39.0 14-15 37.29890630280135 40.0 38.0 41.0 31.0 41.0 16-17 37.31533262548728 40.0 38.0 41.0 31.0 41.0 18-19 37.316540674920375 40.0 37.5 41.0 31.0 41.0 20-21 37.37752155943838 40.0 38.0 41.0 31.0 41.0 22-23 37.34076846475331 40.0 37.5 41.0 31.0 41.0 24-25 37.31723870451892 40.0 37.0 41.0 31.0 41.0 26-27 37.34054082910316 40.0 37.0 41.0 31.0 41.0 28-29 37.326163966345405 40.0 37.0 41.0 31.0 41.0 30-31 37.25311111530553 40.0 37.0 41.0 31.0 41.0 32-33 37.20832283465283 40.0 37.0 41.0 31.0 41.0 34-35 37.14867154927323 40.0 37.0 41.0 31.0 41.0 36-37 36.98460918074362 40.0 37.0 41.0 30.0 41.0 38-39 36.87375238153281 40.0 37.0 41.0 30.0 41.0 40-41 36.801086181356254 40.0 37.0 41.0 30.0 41.0 42-43 36.68801112844973 40.0 36.5 41.0 30.0 41.0 44-45 36.567924056107984 40.0 36.0 41.0 30.0 41.0 46-47 36.4174831001565 40.0 36.0 41.0 30.0 41.0 48-49 36.33725961243268 40.0 36.0 41.0 30.0 41.0 50-51 36.20100580391315 39.5 35.0 41.0 29.0 41.0 52-53 36.07663123343046 39.0 35.0 41.0 29.0 41.0 54-55 35.95236769502189 39.0 35.0 41.0 29.0 41.0 56-57 35.7505352621494 39.0 35.0 41.0 28.0 41.0 58-59 35.571391576980645 39.0 35.0 41.0 28.0 41.0 60-61 35.349078246173065 38.5 35.0 40.0 28.0 41.0 62-63 35.10916135706323 38.0 34.0 40.0 28.0 41.0 64-65 34.75458722220378 37.5 34.0 40.0 27.0 41.0 66-67 31.253993629840327 36.0 31.0 40.0 6.5 41.0 68-69 31.614535525488144 36.0 30.5 39.0 11.0 41.0 70-71 31.779555964940926 35.5 30.0 39.0 15.5 41.0 72-73 33.01958775206498 35.0 32.0 39.0 25.0 40.5 74-75 33.1042868045458 35.0 33.0 38.5 26.0 40.0 76-77 32.80647342575507 35.0 33.0 37.5 25.5 39.5 78-79 29.436213977965963 35.0 29.0 37.0 6.5 39.0 80-81 31.228321342854763 35.0 30.0 36.0 21.5 39.0 82-83 31.64337299408875 35.0 32.0 36.0 23.0 37.5 84-85 29.812715611439362 35.0 30.5 35.5 12.5 37.0 86-87 29.452983743949236 34.5 29.0 35.0 13.5 37.0 88-89 30.705520084923336 34.0 30.5 35.0 20.0 36.0 90-91 30.871119790020327 35.0 32.0 35.0 20.0 36.0 92-93 30.73383036436713 35.0 32.0 35.0 19.5 36.0 94-95 30.536467015116735 35.0 32.0 35.0 16.5 36.0 96-97 30.36046645296356 35.0 32.0 35.0 9.5 35.0 98-99 30.180621327072632 35.0 32.0 35.0 2.0 35.0 100 29.97788159216697 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32734304228963396 1101 2 0.35708251814207514 1101 3 0.3601552565824164 1101 4 0.34510235897000285 1101 5 0.3620578837063775 1101 6 0.3513882530582322 1101 7 -12.154663279234065 1101 8 -6.555542058607269 1101 9 -2.2034570477980964 1101 10-11 -0.0916102428680361 1101 12-13 0.3654253733854418 1101 14-15 0.5682131932439773 1101 16-17 0.6195293475447272 1101 18-19 0.5985804402075701 1101 20-21 0.6097952155765185 1101 22-23 0.5501431935557193 1101 24-25 0.6266603571883351 1101 26-27 0.5830131335017654 1101 28-29 0.5531052233865168 1101 30-31 0.6168262638592168 1101 32-33 0.5855624120893097 1101 34-35 0.650839112194312 1101 36-37 0.6936509223941911 1101 38-39 0.6663680797211669 1101 40-41 0.6765107413251101 1101 42-43 0.6402725042287756 1101 44-45 0.5392061672697821 1101 46-47 0.5780681224967026 1101 48-49 0.48133591484006644 1101 50-51 0.5804397765074896 1101 52-53 0.563289330589626 1101 54-55 0.4700689125381672 1101 56-57 0.5077258080663754 1101 58-59 0.47961005243173815 1101 60-61 0.49005203747397985 1101 62-63 0.4958739534999381 1101 64-65 0.38563636226842846 1101 66-67 -10.282281234060129 1101 68-69 -8.189953260361268 1101 70-71 -6.638790160418772 1101 72-73 -1.4587916001101071 1101 74-75 -0.21304749662129296 1101 76-77 0.06606513538351066 1101 78-79 3.2385828722434447 1101 80-81 1.1726461660830445 1101 82-83 0.44172309186827974 1101 84-85 -5.339763056345877 1101 86-87 -5.869357333383039 1101 88-89 -1.2896405960107522 1101 90-91 0.011702981588417316 1101 92-93 0.3850264009882878 1101 94-95 0.4047047066227485 1101 96-97 0.526916811203133 1101 98-99 0.5663609845090001 1101 100 0.6125694752404414 1102 1 0.11671676148407073 1102 2 0.17434985533457237 1102 3 0.16469257809694682 1102 4 0.14103613141359972 1102 5 0.1287467640115949 1102 6 0.14244849521047342 1102 7 -12.27854736495123 1102 8 -6.720871985097453 1102 9 -2.4282234784121712 1102 10-11 -0.3779469275488694 1102 12-13 0.196843321892608 1102 14-15 0.3455719511196449 1102 16-17 0.3214587684118868 1102 18-19 0.3457246412751971 1102 20-21 0.3228544646258271 1102 22-23 0.32609848510921324 1102 24-25 0.33284019348463545 1102 26-27 0.323121300412879 1102 28-29 0.36453746567543277 1102 30-31 0.3344776815525492 1102 32-33 0.293972042324512 1102 34-35 0.36564922646945774 1102 36-37 0.4885091815139546 1102 38-39 0.45211183571115043 1102 40-41 0.3388381546699435 1102 42-43 0.3473852345225765 1102 44-45 0.3482788641062413 1102 46-47 0.3551055118748394 1102 48-49 0.2656637788659495 1102 50-51 0.33698553289892175 1102 52-53 0.31698080680946106 1102 54-55 0.268199604478653 1102 56-57 0.3156682809457223 1102 58-59 0.2976340336771415 1102 60-61 0.20088054095581498 1102 62-63 0.221520304120304 1102 64-65 0.10955136726342118 1102 66-67 -10.338472514891713 1102 68-69 -6.831193564739895 1102 70-71 -6.366925061441329 1102 72-73 -1.2281724646574332 1102 74-75 -0.2766758061067449 1102 76-77 -0.1514360083010331 1102 78-79 3.023673070868906 1102 80-81 0.9179370720844595 1102 82-83 0.27121273006204305 1102 84-85 -5.557050641118678 1102 86-87 -6.0313721703812995 1102 88-89 -1.5259381857386884 1102 90-91 -0.24380248455335618 1102 92-93 0.09748087885768797 1102 94-95 0.09550284142559562 1102 96-97 0.2229997656857563 1102 98-99 0.25245012143737 1102 100 0.3578222584240258 1103 1 0.1567590380946129 1103 2 0.18545865203868672 1103 3 0.18995175024939925 1103 4 0.12406797980338524 1103 5 0.15993112983709068 1103 6 0.16439733108015986 1103 7 -12.253187896359425 1103 8 -6.679224128628032 1103 9 -2.350643776462178 1103 10-11 -0.3724394100907702 1103 12-13 0.18767758718291816 1103 14-15 0.360784275112934 1103 16-17 0.30527193221741555 1103 18-19 0.33134403319671435 1103 20-21 0.35117665927042196 1103 22-23 0.33888771481237967 1103 24-25 0.447955327151611 1103 26-27 0.38191583000141804 1103 28-29 0.3817840414319349 1103 30-31 0.3398208912916161 1103 32-33 0.3704024147642002 1103 34-35 0.40050028244206004 1103 36-37 0.4784202457691933 1103 38-39 0.47642548317932665 1103 40-41 0.4212584458284141 1103 42-43 0.3945747153926362 1103 44-45 0.36988282174712594 1103 46-47 0.35044465634695143 1103 48-49 0.2813479514577395 1103 50-51 0.30003158671028984 1103 52-53 0.24034168240648057 1103 54-55 0.25811495748348534 1103 56-57 0.2621379299633233 1103 58-59 0.21721479800639543 1103 60-61 0.21543453265810797 1103 62-63 0.15154173088551204 1103 64-65 0.0388519210622178 1103 66-67 -10.438590346594431 1103 68-69 -8.382629381491828 1103 70-71 -6.8954896473519085 1103 72-73 -1.763544785222674 1103 74-75 -0.5732789835274801 1103 76-77 -0.24598619390728516 1103 78-79 2.8919397770929756 1103 80-81 0.796410067040501 1103 82-83 0.14424380020068028 1103 84-85 -5.67155988584145 1103 86-87 -6.119345438512841 1103 88-89 -1.5805693547511623 1103 90-91 -0.3061711485196241 1103 92-93 0.09722066189938161 1103 94-95 0.21370521025274414 1103 96-97 0.2899105723452813 1103 98-99 0.2882550150077101 1103 100 0.3574895942094649 1104 1 0.13975594041951567 1104 2 0.17495867472717563 1104 3 0.1595791127265862 1104 4 0.16158954508147616 1104 5 0.12272025046859625 1104 6 0.18881460642080583 1104 7 -12.231123424317236 1104 8 -6.656232969961181 1104 9 -2.4260836250305715 1104 10-11 -0.388225238506962 1104 12-13 0.18839825950255573 1104 14-15 0.336699612771703 1104 16-17 0.3882800678228904 1104 18-19 0.38917956866698944 1104 20-21 0.42704489765829834 1104 22-23 0.444876473457434 1104 24-25 0.4968215201286057 1104 26-27 0.445285727529253 1104 28-29 0.43011745039355986 1104 30-31 0.38245339265234435 1104 32-33 0.3890326087458149 1104 34-35 0.3737067421119633 1104 36-37 0.4507522719843422 1104 38-39 0.46636890526721686 1104 40-41 0.4704033282805611 1104 42-43 0.45205432373030874 1104 44-45 0.4219281240236583 1104 46-47 0.4092776530674911 1104 48-49 0.3878149508511157 1104 50-51 0.3905764142756567 1104 52-53 0.34102668823395277 1104 54-55 0.3672828077211392 1104 56-57 0.3518533055952062 1104 58-59 0.32790166140956245 1104 60-61 0.3173077034430207 1104 62-63 0.24473170327260618 1104 64-65 0.16791784686750333 1104 66-67 -10.41967535424454 1104 68-69 -8.36328493690625 1104 70-71 -6.842465906977338 1104 72-73 -1.7291306574670813 1104 74-75 -0.533180722542248 1104 76-77 -0.23253443485062064 1104 78-79 2.9398138622168766 1104 80-81 0.8189649031802766 1104 82-83 0.13943331541828385 1104 84-85 -5.577448804514717 1104 86-87 -6.083644063684897 1104 88-89 -1.539676725176168 1104 90-91 -0.25081608926300447 1104 92-93 0.07378343008569033 1104 94-95 0.14654151302360674 1104 96-97 0.2809248096194601 1104 98-99 0.3503062179437926 1104 100 0.3911842537171175 1105 1 0.043713590940075875 1105 2 0.024304632664261305 1105 3 0.039122290434686846 1105 4 0.09632703748820859 1105 5 0.07031011819201893 1105 6 0.1660918457878111 1105 7 -12.328089487184723 1105 8 -6.781063364001302 1105 9 -2.5248952806066356 1105 10-11 -0.4692869018495074 1105 12-13 0.040104492515354195 1105 14-15 0.19967874932531515 1105 16-17 0.259979187823717 1105 18-19 0.23540515507724535 1105 20-21 0.2800192977891598 1105 22-23 0.26090481745386995 1105 24-25 0.2842263422136426 1105 26-27 0.2335895483764432 1105 28-29 0.2515083134586362 1105 30-31 0.2631676111498322 1105 32-33 0.2798778086469511 1105 34-35 0.2721860669178966 1105 36-37 0.3442736367877899 1105 38-39 0.3342029996857434 1105 40-41 0.34452608925773376 1105 42-43 0.2546242915947303 1105 44-45 0.20882678875911154 1105 46-47 0.17754211256145425 1105 48-49 0.137731127172664 1105 50-51 0.22295253795901004 1105 52-53 0.22599278470336515 1105 54-55 0.14384760016402254 1105 56-57 0.0806640291932581 1105 58-59 0.13649227290397903 1105 60-61 0.15819757987033967 1105 62-63 0.07118338129947688 1105 64-65 -0.08573323813514122 1105 66-67 -10.522613537202602 1105 68-69 -8.532997193449642 1105 70-71 -6.982922556278179 1105 72-73 -1.8531359475518023 1105 74-75 -0.6509134790696507 1105 76-77 -0.3530919044279699 1105 78-79 2.8704749018134486 1105 80-81 0.7102282591543023 1105 82-83 0.047804264495241 1105 84-85 -5.703946139501397 1105 86-87 -6.21458889919078 1105 88-89 -1.664284302655556 1105 90-91 -0.37143145650264486 1105 92-93 -0.028170220899681908 1105 94-95 0.0456871477662979 1105 96-97 0.10502882461515739 1105 98-99 0.1287715119067805 1105 100 0.21569938287214896 1106 1 0.0935586724557993 1106 2 0.1512196210144623 1106 3 0.11866251519365889 1106 4 0.08841949867011323 1106 5 0.12083441517506799 1106 6 0.1355272484239265 1106 7 -12.258017288803817 1106 8 -6.737905401917828 1106 9 -2.532652145763052 1106 10-11 -0.456650913498045 1106 12-13 0.126801151616327 1106 14-15 0.29851378670497297 1106 16-17 0.26452448071669465 1106 18-19 0.2970816490782937 1106 20-21 0.27937713681662757 1106 22-23 0.2877893443782611 1106 24-25 0.35779009355105273 1106 26-27 0.29747655676799667 1106 28-29 0.2959349808813059 1106 30-31 0.2701165403209771 1106 32-33 0.2737210563379122 1106 34-35 0.31580142179186055 1106 36-37 0.37070622494866257 1106 38-39 0.3106572362226103 1106 40-41 0.313577122768983 1106 42-43 0.3245901501532984 1106 44-45 0.2236218098716165 1106 46-47 0.23707060792838774 1106 48-49 0.200620722844981 1106 50-51 0.2589957136370913 1106 52-53 0.2639685496166777 1106 54-55 0.18728768618131397 1106 56-57 0.2153210115690385 1106 58-59 0.17069143932255315 1106 60-61 0.16182780214776216 1106 62-63 0.11275709380597476 1106 64-65 0.02782192204091416 1106 66-67 -10.485683964290953 1106 68-69 -8.382666823028792 1106 70-71 -6.942843121088664 1106 72-73 -1.8360770667317325 1106 74-75 -0.5879846144031475 1106 76-77 -0.2999633561586137 1106 78-79 2.8296135235993596 1106 80-81 0.7385302575404786 1106 82-83 0.06804178512242842 1106 84-85 -5.693885581055522 1106 86-87 -6.10487969409564 1106 88-89 -1.6416233961429967 1106 90-91 -0.4148771500270705 1106 92-93 -0.052552007216625896 1106 94-95 0.023145139041695018 1106 96-97 0.10071076457451156 1106 98-99 0.13468363877295175 1106 100 0.22632547184126395 1107 1 0.10153165028556188 1107 2 0.1413230690974423 1107 3 0.14475666870758275 1107 4 0.13456287037463 1107 5 0.14201951194913676 1107 6 0.1317279991312006 1107 7 -12.291843093365191 1107 8 -6.730229815064909 1107 9 -2.502240824241902 1107 10-11 -0.4299647587373343 1107 12-13 0.1435111908811919 1107 14-15 0.27503663199627226 1107 16-17 0.30470305932091435 1107 18-19 0.3089904362342537 1107 20-21 0.27829589189061466 1107 22-23 0.36113362308828556 1107 24-25 0.42965818658856136 1107 26-27 0.3532232845832084 1107 28-29 0.31898086884587684 1107 30-31 0.3229828576279843 1107 32-33 0.3520058957503238 1107 34-35 0.28806616900567406 1107 36-37 0.4234933644396506 1107 38-39 0.4325832933364637 1107 40-41 0.4194027905131392 1107 42-43 0.41530913744362863 1107 44-45 0.39254779285472097 1107 46-47 0.43597076280572367 1107 48-49 0.3686544525303077 1107 50-51 0.39472553732612425 1107 52-53 0.36317729223630835 1107 54-55 0.3205648942410093 1107 56-57 0.30920502195146327 1107 58-59 0.2822147915704818 1107 60-61 0.2831559590585826 1107 62-63 0.2569353640571208 1107 64-65 0.11022190226414352 1107 66-67 -10.378664762497205 1107 68-69 -8.300074331091718 1107 70-71 -6.771725876777552 1107 72-73 -1.7063383644545311 1107 74-75 -0.35836337047111044 1107 76-77 -0.09725429184494772 1107 78-79 3.041370252701096 1107 80-81 0.929984483022313 1107 82-83 0.2590884521992862 1107 84-85 -5.5697500192476035 1107 86-87 -6.042521023925477 1107 88-89 -1.4856178150552992 1107 90-91 -0.2203015730175295 1107 92-93 0.1614996408938829 1107 94-95 0.23700560953473016 1107 96-97 0.3059064720117419 1107 98-99 0.3284061334830177 1107 100 0.4249326559405837 1108 1 -0.035150672398305716 1108 2 0.0052733620294667105 1108 3 0.018223350679107853 1108 4 -0.048096369406728456 1108 5 -0.04472440147367962 1108 6 -0.02987015940198745 1108 7 -12.291119699532345 1108 8 -6.797151359275066 1108 9 -2.685203360811208 1108 10-11 -0.6122828486302367 1108 12-13 -0.03504693448979168 1108 14-15 0.11132883175291397 1108 16-17 0.08371174986416463 1108 18-19 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1.0141650818267358 2214 82-83 0.2784933731751842 2214 84-85 1.8539044236860782 2214 86-87 2.020446615925426 2214 88-89 0.5769172437276637 2214 90-91 0.1790700026515033 2214 92-93 0.09246762518990437 2214 94-95 0.1729007011127699 2214 96-97 0.06801619661058211 2214 98-99 0.10170748016160402 2214 100 0.09977781158651666 2215 1 -0.2769095181764101 2215 2 -0.45059222859977766 2215 3 -0.34986572788076487 2215 4 -0.2877025893691538 2215 5 -0.25320281383145016 2215 6 -0.37244454558653217 2215 7 3.571614466564302 2215 8 1.6915732456766008 2215 9 0.34539799845001085 2215 10-11 -0.29122278292119574 2215 12-13 -0.5370993386853513 2215 14-15 -0.6234734602274798 2215 16-17 -0.6927918168837266 2215 18-19 -0.6292611895292879 2215 20-21 -0.6842312794320051 2215 22-23 -0.5973920140695057 2215 24-25 -0.5381515204484799 2215 26-27 -0.5961611710770214 2215 28-29 -0.5067518072420825 2215 30-31 -0.49622580815667305 2215 32-33 -0.5295570322939582 2215 34-35 -0.5738591677501574 2215 36-37 -0.5998108813850038 2215 38-39 -0.5501201813224128 2215 40-41 -0.5614506983340561 2215 42-43 -0.5411209309318536 2215 44-45 -0.5532049409532505 2215 46-47 -0.476481682139422 2215 48-49 -0.5138947961308915 2215 50-51 -0.4429467937748939 2215 52-53 -0.42712316669810946 2215 54-55 -0.5253831046651101 2215 56-57 -0.3558057585165315 2215 58-59 -0.40363288500176964 2215 60-61 -0.3843846719259645 2215 62-63 -0.452330348818073 2215 64-65 -0.3287820669654451 2215 66-67 2.9764127679581236 2215 68-69 2.2935874978358335 2215 70-71 1.8473538643829528 2215 72-73 0.20135278435305537 2215 74-75 -0.13856244651449146 2215 76-77 -0.22812900752565213 2215 78-79 2.835613954381568 2215 80-81 0.6329958701309657 2215 82-83 -0.07623073642966816 2215 84-85 1.6151468160958977 2215 86-87 1.7951983727707663 2215 88-89 0.3595037215109116 2215 90-91 -0.06687987531625339 2215 92-93 -0.32559581771403145 2215 94-95 -0.23327339133235725 2215 96-97 -0.281753552115223 2215 98-99 -0.3799993488728468 2215 100 -0.2842138852196605 2216 1 -0.6649258435867154 2216 2 -0.4909455322848508 2216 3 -0.6198162824355542 2216 4 -0.5979040444093044 2216 5 -0.4385533300821365 2216 6 -0.6086011095271644 2216 7 3.453096736060335 2216 8 1.5292887814941025 2216 9 0.25748660845306404 2216 10-11 -0.4528080967178525 2216 12-13 -0.7812178951088455 2216 14-15 -1.010768822025824 2216 16-17 -1.1140554701688359 2216 18-19 -1.5412230214020184 2216 20-21 -1.1582667274639746 2216 22-23 -0.9640081575922963 2216 24-25 -1.027497446464615 2216 26-27 -1.3581535219723762 2216 28-29 -1.5125188413473083 2216 30-31 -1.384629400805018 2216 32-33 -2.258218791171352 2216 34-35 -1.397018098975657 2216 36-37 -1.1118160632958194 2216 38-39 -1.2615180688224257 2216 40-41 -1.2893737666044345 2216 42-43 -0.9850030452800667 2216 44-45 -1.0735122347262376 2216 46-47 -1.2388014413613888 2216 48-49 -1.3088487961157327 2216 50-51 -1.723204867432699 2216 52-53 -1.4676684157764583 2216 54-55 -1.1119830894058538 2216 56-57 -1.1640407642178516 2216 58-59 -1.4042863120865263 2216 60-61 -1.5079064434750222 2216 62-63 -1.406602801550605 2216 64-65 -1.119106179715473 2216 66-67 2.2415522490436643 2216 68-69 1.415239275092926 2216 70-71 1.0879796979366034 2216 72-73 -0.6470450441852691 2216 74-75 -1.4084897457909769 2216 76-77 -1.0724393978770337 2216 78-79 1.4892864729176658 2216 80-81 3.972323119505461E-4 2216 82-83 -0.7944685373629632 2216 84-85 0.927120470797167 2216 86-87 0.895310948811403 2216 88-89 -0.35021874877316606 2216 90-91 -0.69062273070994 2216 92-93 -0.8128036951797633 2216 94-95 -0.7628332368179365 2216 96-97 -0.745722441216401 2216 98-99 -0.7983611183089678 2216 100 -1.8463775859040474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118882.0 3 23483.0 4 13639.0 5 12189.0 6 11085.0 7 12068.0 8 13709.0 9 15814.0 10 17951.0 11 20149.0 12 22670.0 13 25835.0 14 29348.0 15 32279.0 16 34268.0 17 35682.0 18 33981.0 19 32199.0 20 31529.0 21 31445.0 22 33634.0 23 38405.0 24 46348.0 25 58327.0 26 74224.0 27 90818.0 28 112645.0 29 142717.0 30 176441.0 31 217912.0 32 290977.0 33 410767.0 34 626270.0 35 862841.0 36 969229.0 37 1616932.0 38 1839199.0 39 617338.0 40 1527.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83771690802181 20.473738128229225 14.597831397427626 23.090713566321337 2 15.315598661166879 20.19178070353779 40.591906963924366 23.90071367137096 3 18.537950833400703 24.8902483549903 30.27691105559996 26.294889756009038 4 12.7304238509423 16.770276241410308 38.64730377458596 31.851996133061437 5 13.301528907055626 37.7060742959615 35.349316732811545 13.64308006417132 6 30.91203863895386 37.61204378993503 17.789624150073895 13.68629342103722 7 27.30299697029281 32.5306316471086 22.298614035069658 17.867757347528933 8 24.817681085792735 36.553609117435236 20.507839104993728 18.120870691778304 9 24.6713496087896 17.436924912982235 21.842584376417037 36.04914110181113 10-11 23.81273568021671 27.28384391789835 28.76469796319534 20.1387224386896 12-13 24.105216790551097 24.66195764839866 28.921103666775977 22.31172189427427 14-15 21.541774439222646 26.999293670000625 26.102600231319663 25.356331659457066 16-17 21.137688757840774 30.33856396338991 26.896782779331357 21.62696449943796 18-19 21.130938709385457 29.370826205980016 28.682745718016513 20.815489366618017 20-21 22.180535764721615 28.54704553486191 28.284775609465456 20.987643090951018 22-23 21.9276988427933 28.301929485134636 28.367087635352682 21.403284036719384 24-25 21.732257267853708 28.42084533101316 28.2873623782172 21.55953502291593 26-27 21.458728242542165 28.501151199074265 28.342744068814746 21.697376489568825 28-29 21.442283333386396 28.54802907550818 28.532275369549765 21.477412221555664 30-31 21.78087714997437 28.825757076148562 28.06280810974176 21.33055766413531 32-33 21.459916817462048 28.612276783848827 28.4471215199924 21.480684878696728 34-35 21.672515609308824 28.42994890589297 28.37659322286746 21.52094226193075 36-37 21.848729367529778 28.549490980645963 28.096505030157754 21.505274621666505 38-39 21.646882996777567 28.674868488355017 28.251155646374855 21.42709286849256 40-41 21.602827866969818 28.438759415269736 28.350621665910914 21.607791051849535 42-43 21.548307181093822 28.464146266293877 28.31229436959205 21.675252183020252 44-45 21.65925835804744 28.573468751359123 28.19219550771384 21.575077382879602 46-47 21.637730114452815 28.644113808672238 28.168024448892215 21.550131627982736 48-49 21.583867916783497 28.497124062702323 28.184799523531108 21.73420849698307 50-51 21.604062115394587 28.50192183317096 28.11934604458489 21.774670006849558 52-53 21.59615629079856 28.535786301211612 28.175138668207588 21.69291873978224 54-55 21.679962045419853 28.70097947945487 28.016677046944917 21.60238142818037 56-57 21.588416480824158 28.56353414633675 28.020703081072284 21.82734629176681 58-59 21.555544412516227 28.297333356983867 28.298663699434478 21.84845853106543 60-61 21.69104060906202 28.562778820458558 28.02555943746633 21.720621133013093 62-63 21.529242187671894 28.611745769011034 28.061177237641218 21.79783480567586 64-65 21.62936725677628 28.404349204640017 28.0968105040493 21.8694730345344 66-67 21.476424826271323 28.445848651563153 28.06920461428485 22.00852190788067 68-69 21.92148608616431 28.56509508177122 27.888068921151294 21.62534991091318 70-71 21.678216837259427 28.378488858587232 27.996632225186314 21.94666207896703 72-73 21.564864577835195 28.471470028469593 28.177359270628973 21.786306123066236 74-75 21.61887512478686 28.551251177512114 28.032790510837145 21.797083186863876 76-77 21.6588608834602 28.498080132677938 27.878304262654108 21.964754721207747 78-79 21.778007983990662 28.47478873297006 28.052483089380736 21.69472019365854 80-81 21.607900349033095 28.44610625411133 28.085179084849894 21.86081431200568 82-83 21.567060151659234 28.44178078102903 28.222269803433374 21.76888926387836 84-85 21.67256136614565 28.321311526869025 28.006607617050385 21.999519489934944 86-87 21.75254008310813 28.45138690649832 27.978537364460625 21.81753564593292 88-89 21.599264732009633 28.318596950759595 28.08462995515495 21.99750836207582 90-91 21.575247585940783 28.2475530223732 28.04599457738876 22.131204814297256 92-93 21.7192897952816 28.343192992103404 27.98054266670396 21.956974545911034 94-95 21.624006900322552 28.165509041777458 28.13717842144831 22.07330563645168 96-97 21.593759668719056 28.23758887546252 28.06681108319331 22.101840372625112 98-99 21.64167601888801 28.241182185213727 28.024933091273834 22.092208704624433 100 21.729365141906065 28.113548958732387 27.968533469604456 22.18855242975709 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1913.0 1 1637.5 2 1574.5 3 1819.0 4 1738.0 5 1509.5 6 1374.0 7 1403.5 8 1464.5 9 1444.0 10 1365.0 11 1332.5 12 1370.5 13 1429.5 14 1590.5 15 1823.0 16 2255.5 17 2962.0 18 3997.0 19 5633.0 20 8224.5 21 12039.0 22 18555.5 23 28776.0 24 40506.5 25 53742.0 26 71410.0 27 93556.0 28 120101.5 29 146251.0 30 167596.0 31 189188.5 32 211501.5 33 232024.5 34 252016.0 35 269280.5 36 281369.5 37 292944.5 38 302495.5 39 306728.0 40 310280.5 41 310515.0 42 306098.0 43 303257.0 44 298889.0 45 293647.5 46 289561.5 47 282301.0 48 274332.0 49 270299.0 50 265372.5 51 256164.0 52 245439.5 53 235205.0 54 226964.5 55 216417.5 56 198191.0 57 176034.5 58 156885.5 59 140119.5 60 120859.5 61 100387.0 62 83108.0 63 67989.5 64 54698.0 65 43008.0 66 33322.0 67 25727.0 68 19298.5 69 14426.0 70 10691.0 71 8005.0 72 6192.5 73 4383.0 74 2977.5 75 1996.5 76 1299.0 77 903.5 78 658.5 79 486.5 80 329.0 81 192.0 82 137.5 83 104.5 84 89.0 85 80.0 86 57.5 87 43.5 88 33.5 89 28.5 90 29.5 91 23.5 92 18.5 93 18.0 94 18.0 95 20.5 96 19.5 97 15.5 98 19.5 99 25.5 100 45.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018511030891590396 2 0.002637935606172587 3 0.0014440423361375802 4 0.0022968232433054426 5 0.001967081292533869 6 0.0035816798101050216 7 11.639981825533306 8 2.2740824191142993E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.501490661025729E-4 18-19 0.0 20-21 0.0 22-23 5.685206047785748E-6 24-25 0.0 26-27 1.4781535724242947E-4 28-29 0.0 30-31 0.0 32-33 1.7055618143357246E-5 34-35 4.5481648382285986E-5 36-37 2.2740824191142993E-5 38-39 2.842603023892874E-5 40-41 0.0 42-43 2.842603023892874E-5 44-45 1.5350056329021522E-4 46-47 9.494294099802201E-4 48-49 6.594839015431468E-4 50-51 3.1837153867600193E-4 52-53 2.501490661025729E-4 54-55 3.8090880520164517E-4 56-57 0.0013985606877552942 58-59 3.1837153867600193E-4 60-61 0.0013417086272774369 62-63 0.0023934717461178002 64-65 0.0010290222946492205 66-67 5.820161468948086 68-69 5.465478519244877 70-71 0.002558342721503587 72-73 0.0012507453305128646 74-75 0.002961992350896375 76-77 0.006009262792509537 78-79 5.419252108870332 80-81 0.00524175997605846 82-83 0.004946129261573602 84-85 5.820411618014189 86-87 0.003519142543579379 88-89 0.0015861724873322237 90-91 0.003234882241190091 92-93 0.0037977176399208805 94-95 0.0043719234507472405 96-97 0.0032633082714290194 98-99 0.005679520841737963 100 0.0059467255259838935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8794756.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.26108940969743 #Duplication Level Percentage of deduplicated Percentage of total 1 89.62689933295907 75.52060178208416 2 7.174239359559091 12.09018448244758 3 1.679441715106693 4.24534765544942 4 0.6381007068169562 2.150682428379787 5 0.3063452007728048 1.290649017627451 6 0.1771613968358113 0.8956687379237511 7 0.11186390306630781 0.6598042036591523 8 0.06969635218341222 0.4698152450285007 9 0.050078254753546124 0.379768347114314 >10 0.163201519636953 2.095699125047586 >50 0.0024047146936564154 0.13318400108438355 >100 5.675436156577368E-4 0.0685949741538658 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.1370412095571498E-5 0.0 8 0.0 0.0 0.0 1.1370412095571498E-5 0.0 9 1.1370412095571498E-5 1.1370412095571498E-5 0.0 1.1370412095571498E-5 0.0 10-11 1.1370412095571498E-5 1.1370412095571498E-5 0.0 2.2740824191142996E-5 0.0 12-13 1.1370412095571498E-5 1.1370412095571498E-5 0.0 4.548164838228599E-5 0.0 14-15 1.1370412095571498E-5 2.8426030238928747E-5 0.0 5.685206047785749E-5 0.0 16-17 1.1370412095571498E-5 4.548164838228599E-5 0.0 6.822247257342898E-5 0.0 18-19 1.1370412095571498E-5 4.548164838228599E-5 0.0 6.822247257342898E-5 0.0 20-21 1.1370412095571498E-5 4.548164838228599E-5 0.0 7.959288466900048E-5 0.0 22-23 1.1370412095571498E-5 4.548164838228599E-5 0.0 2.103526237680727E-4 0.0 24-25 2.2740824191142996E-5 5.1166854430071743E-5 0.0 4.207052475361454E-4 0.0 26-27 2.2740824191142996E-5 5.685206047785749E-5 0.0 8.755217313590052E-4 0.0 28-29 2.2740824191142996E-5 5.685206047785749E-5 0.0 0.0029108254964663035 0.0 30-31 2.2740824191142996E-5 5.685206047785749E-5 0.0 0.008931458701071412 0.0 32-33 2.2740824191142996E-5 5.685206047785749E-5 0.0 0.02183687642954506 0.0 34-35 2.2740824191142996E-5 5.685206047785749E-5 0.0 0.0426845270067754 0.0 36-37 2.2740824191142996E-5 5.685206047785749E-5 0.0 0.0775518956978454 0.0 38-39 2.2740824191142996E-5 7.390767862121473E-5 0.0 0.12430134502878762 0.0 40-41 2.2740824191142996E-5 9.664850281235774E-5 0.0 0.17223331721766927 0.0 42-43 2.2740824191142996E-5 1.0233370886014347E-4 0.0 0.22039269764846234 0.0 44-45 2.2740824191142996E-5 1.0233370886014347E-4 0.0 0.27989406414458795 0.0 46-47 3.411123628671449E-5 1.0233370886014347E-4 0.0 0.35364824220251245 0.0 48-49 3.411123628671449E-5 1.0233370886014347E-4 0.0 0.4311660266640712 0.0 50-51 3.411123628671449E-5 1.1370412095571497E-4 0.0 0.5077969189821753 0.0 52-53 3.411123628671449E-5 1.2507453305128646E-4 0.0 0.5850588691715837 0.0 54-55 4.548164838228599E-5 1.2507453305128646E-4 0.0 0.6696831611928744 0.0 56-57 5.685206047785749E-5 1.2507453305128646E-4 0.0 0.7624600386866902 0.0 58-59 6.822247257342898E-5 1.2507453305128646E-4 0.0 0.8642365973541506 0.0 60-61 6.822247257342898E-5 1.2507453305128646E-4 0.0 0.9682815532346776 0.0 62-63 6.822247257342898E-5 1.2507453305128646E-4 0.0 1.0733669018219494 0.0 64-65 6.822247257342898E-5 1.3075973909907221E-4 0.0 1.18787832203645 0.0 66-67 6.822247257342898E-5 1.4213015119464372E-4 0.0 1.314556083193212 0.0 68-69 6.822247257342898E-5 1.5350056329021522E-4 0.0 1.4412850112044042 0.0 70-71 6.822247257342898E-5 1.7055618143357247E-4 0.0 1.5914483585445691 0.0 72-73 6.822247257342898E-5 1.989822116725012E-4 0.0 1.7522487264001412 0.0 74-75 6.822247257342898E-5 2.0466741772028695E-4 0.0 1.900029972406284 0.0 76-77 6.822247257342898E-5 2.0466741772028695E-4 0.0 2.054707373348391 0.0 78-79 6.822247257342898E-5 2.0466741772028695E-4 0.0 2.2361620947755685 0.0 80-81 6.822247257342898E-5 2.0466741772028695E-4 0.0 2.454377358507729 0.0 82-83 6.822247257342898E-5 2.2740824191142995E-4 0.0 2.6784029028207264 0.0 84-85 6.822247257342898E-5 2.501490661025729E-4 0.0 2.9037872113791448 0.0 86-87 6.822247257342898E-5 2.6151947819814443E-4 0.0 3.167955995595557 0.0 88 6.822247257342898E-5 2.6151947819814443E-4 0.0 3.3917370760485 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7950 0.0 43.474533 1 GTATCAA 12735 0.0 37.27169 1 TCAACGC 13210 0.0 34.413162 4 ATCAACG 13395 0.0 34.052536 3 CAACGCA 14975 0.0 30.528051 5 TATCAAC 15445 0.0 29.897377 2 AACGCAG 16460 0.0 27.742393 6 GTACATG 24570 0.0 24.48674 1 ACGCAGA 19185 0.0 23.72156 7 CGCAGAG 19330 0.0 23.470383 8 TACATGG 25360 0.0 22.871525 2 ACATGGG 25905 0.0 21.95558 3 GCAGAGT 22690 0.0 19.097746 9 CATGGGG 18850 0.0 18.73925 4 GAGTACA 18900 0.0 18.368183 1 GTGGTAT 5060 0.0 18.315819 1 GTCGTAC 425 1.8189894E-11 18.071768 1 CAGAGTA 21445 0.0 16.641867 10-11 GTATAGG 2845 0.0 16.377869 1 AGAGTAC 19650 0.0 16.159416 10-11 >>END_MODULE