FastQCFastQC Report
Wed 25 May 2016
SRR2049531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794756
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257960.29331115041736233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245950.27965528549058094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145880.165871571650197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159000.057.410071
GTATCAA286500.040.5534631
ATCAACG358350.032.195343
TCAACGC359250.032.1408774
TATCAAC372500.031.0228752
CAACGCA373850.030.9637015
GTGGTAT62400.030.3283541
AACGCAG397200.029.2021186
TGGTATC70600.025.9253622
CGCAGAG451700.025.4181778
ACGCAGA454550.025.3725387
GCAGAGT488600.023.1041819
GTACATG251400.022.845571
GAGTACT341500.021.67508712-13
TACATGG259750.021.5380442
CAGAGTA476550.020.71008310-11
ACATGGG264100.020.363753
AGAGTAC458350.020.32251410-11
GTACTTT373650.019.6214314-15
AGTACTT359400.019.42520512-13
Produced by FastQC (version 0.11.5)