##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049531_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8794756
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.785343675253753	31.0	31.0	34.0	30.0	34.0
2	31.982144814478083	33.0	31.0	34.0	30.0	34.0
3	32.00680450941447	34.0	31.0	34.0	30.0	34.0
4	35.61467845156818	37.0	35.0	37.0	33.0	37.0
5	35.73015908570971	37.0	35.0	37.0	33.0	37.0
6	35.79864955889623	37.0	35.0	37.0	35.0	37.0
7	35.775866664180334	37.0	35.0	37.0	33.0	37.0
8	35.776939235153314	37.0	35.0	37.0	33.0	37.0
9	37.5200205668014	39.0	37.0	39.0	35.0	39.0
10-11	37.474034470086494	39.0	37.0	39.0	34.5	39.0
12-13	37.45178774715296	39.0	37.0	39.0	34.0	39.0
14-15	38.72399154678084	40.0	38.0	41.0	34.0	41.0
16-17	38.736004671420105	40.0	38.0	41.0	34.5	41.0
18-19	38.80613436006638	40.0	38.0	41.0	35.0	41.0
20-21	38.83628459959549	40.0	38.0	41.0	35.0	41.0
22-23	38.81451657101118	40.0	38.0	41.0	35.0	41.0
24-25	38.777817713191816	40.0	38.0	41.0	35.0	41.0
26-27	38.685353010362086	40.0	38.0	41.0	34.0	41.0
28-29	38.60229078555449	40.0	38.0	41.0	34.0	41.0
30-31	38.49274488115418	40.0	38.0	41.0	34.0	41.0
32-33	38.35439874625288	40.0	38.0	41.0	34.0	41.0
34-35	38.257665931834836	40.0	38.0	41.0	34.0	41.0
36-37	38.126791976946265	40.0	38.0	41.0	33.5	41.0
38-39	38.040356491982266	40.0	38.0	41.0	33.0	41.0
40-41	37.923690208119474	40.0	37.5	41.0	33.0	41.0
42-43	37.78449839881857	40.0	37.0	41.0	33.0	41.0
44-45	37.60102764647478	40.0	37.0	41.0	33.0	41.0
46-47	37.36144271654609	40.0	37.0	41.0	32.5	41.0
48-49	37.13583190937872	40.0	37.0	41.0	31.5	41.0
50-51	36.9258970914031	40.0	36.5	41.0	31.0	41.0
52-53	36.82250138605323	40.0	36.0	41.0	31.0	41.0
54-55	36.69307727241097	39.5	36.0	41.0	31.0	41.0
56-57	36.52172396823744	39.0	35.5	41.0	31.0	41.0
58-59	36.35217935551594	39.0	35.0	41.0	30.5	41.0
60-61	36.16612422220696	39.0	35.0	41.0	30.0	41.0
62-63	35.97301562431067	39.0	35.0	41.0	30.0	41.0
64-65	35.704010946977945	38.0	35.0	40.0	29.5	41.0
66-67	35.45152219117847	37.5	35.0	40.0	29.0	41.0
68-69	35.14943819930877	37.0	35.0	40.0	29.0	41.0
70-71	34.86350326262604	37.0	34.0	39.5	29.0	41.0
72-73	34.54893057863117	36.0	34.0	39.0	29.0	41.0
74-75	34.21011424307849	36.0	34.0	39.0	28.0	40.5
76-77	33.91750851302754	35.0	34.0	38.5	28.0	40.0
78-79	33.59201301320923	35.0	34.0	37.0	28.0	39.0
80-81	33.284796928988136	35.0	34.0	37.0	28.0	39.0
82-83	32.99248688650373	35.0	34.0	36.0	27.0	39.0
84-85	32.71704774981819	35.0	33.0	36.0	27.0	37.0
86-87	32.44507357566259	35.0	33.0	36.0	26.5	37.0
88-89	32.23480071533537	35.0	33.0	35.0	26.0	37.0
90-91	32.084866538650985	35.0	33.0	35.0	26.0	36.0
92-93	31.927939785936076	35.0	33.0	35.0	26.0	36.0
94-95	31.74578333952642	35.0	33.0	35.0	26.0	36.0
96-97	31.580608489877378	35.0	33.0	35.0	25.0	36.0
98-99	31.401833490320826	35.0	33.0	35.0	25.0	35.0
100	31.291075954807614	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.403189730703609
1101	2	0.43724850234700696
1101	3	0.5193919744145461
1101	4	0.39933971282389535
1101	5	0.32613157411451255
1101	6	0.3258728162510707
1101	7	0.31878125764637133
1101	8	0.35262365957328257
1101	9	0.37741813097484567
1101	10-11	0.3725885081443252
1101	12-13	0.35196005171705025
1101	14-15	0.48835273620395725
1101	16-17	0.5047293511875637
1101	18-19	0.4600211969821757
1101	20-21	0.43065459160763453
1101	22-23	0.44694666477082734
1101	24-25	0.4961405444551801
1101	26-27	0.48316869987981903
1101	28-29	0.4598676071104464
1101	30-31	0.5083316387996106
1101	32-33	0.5086068185298771
1101	34-35	0.5500741753593346
1101	36-37	0.5785095534260449
1101	38-39	0.5378594356131998
1101	40-41	0.5618143350522402
1101	42-43	0.45341543296900255
1101	44-45	0.5154894470034819
1101	46-47	0.561836469381646
1101	48-49	0.5520716785224025
1101	50-51	0.6318852097335395
1101	52-53	0.6304307784797913
1101	54-55	0.5384530886850598
1101	56-57	0.5174164459543746
1101	58-59	0.5086930690604845
1101	60-61	0.5466289939103532
1101	62-63	0.5578913618389052
1101	64-65	0.5659993471291216
1101	66-67	0.6166163430452158
1101	68-69	0.5709092776937084
1101	70-71	0.5544225918482013
1101	72-73	0.5421859474678499
1101	74-75	0.5502772528682982
1101	76-77	0.5555234509717408
1101	78-79	0.5587396991216096
1101	80-81	0.5107421294294312
1101	82-83	0.49748310354899417
1101	84-85	0.4915598140007873
1101	86-87	0.5470259892929334
1101	88-89	0.539267398199847
1101	90-91	0.5526744459582886
1101	92-93	0.5427281941539981
1101	94-95	0.515468557127253
1101	96-97	0.5601854989328316
1101	98-99	0.6034437685092051
1101	100	0.6649643687021758
1102	1	0.315709116691572
1102	2	0.36848451933446214
1102	3	0.42208342100251883
1102	4	0.3921903419458772
1102	5	0.2999626147086403
1102	6	0.27268462851357356
1102	7	0.2783672834477713
1102	8	0.294585024144844
1102	9	0.36351875281061297
1102	10-11	0.3227744204833911
1102	12-13	0.2998523885731146
1102	14-15	0.40381691601969294
1102	16-17	0.4159984958719889
1102	18-19	0.3867492788739568
1102	20-21	0.3792373173789869
1102	22-23	0.37620777048147147
1102	24-25	0.38691410865655484
1102	26-27	0.3548422901731172
1102	28-29	0.41036248152994403
1102	30-31	0.4544634531619849
1102	32-33	0.48178853442399117
1102	34-35	0.5049624337852094
1102	36-37	0.4881848291721198
1102	38-39	0.5001758824799367
1102	40-41	0.4960286771575042
1102	42-43	0.5144922564002243
1102	44-45	0.5376966228136126
1102	46-47	0.48596146580263166
1102	48-49	0.541916417339003
1102	50-51	0.5847584186628154
1102	52-53	0.5222018096445353
1102	54-55	0.522216222345051
1102	56-57	0.5056849033386825
1102	58-59	0.4960623175358734
1102	60-61	0.5506596792605691
1102	62-63	0.5517036645137523
1102	64-65	0.5207614747158473
1102	66-67	0.5839907663294568
1102	68-69	0.5412578030436492
1102	70-71	0.5514667117076186
1102	72-73	0.60282422712433
1102	74-75	0.5501465433178794
1102	76-77	0.5172123324304536
1102	78-79	0.47531468582636904
1102	80-81	0.43847500614509727
1102	82-83	0.44059820082824785
1102	84-85	0.46901942079581005
1102	86-87	0.4555582309435877
1102	88-89	0.442178100654516
1102	90-91	0.42826638457092514
1102	92-93	0.4964984267709873
1102	94-95	0.5450353816912106
1102	96-97	0.569069913009919
1102	98-99	0.5325471794305763
1102	100	0.6169139435888589
1103	1	0.322672999980675
1103	2	0.34418751615269016
1103	3	0.4282739633657897
1103	4	0.3665289379995187
1103	5	0.2725740445450171
1103	6	0.2736354510649832
1103	7	0.26959043433708274
1103	8	0.2506134283918229
1103	9	0.320912849984893
1103	10-11	0.28658230266611184
1103	12-13	0.294870658277155
1103	14-15	0.42122220012345224
1103	16-17	0.3793262411553613
1103	18-19	0.40113984275850356
1103	20-21	0.40014777129039203
1103	22-23	0.3724694391415184
1103	24-25	0.39972773562242025
1103	26-27	0.39384883068236576
1103	28-29	0.3987128901167267
1103	30-31	0.42621768071229127
1103	32-33	0.3893891413515931
1103	34-35	0.3436138596756919
1103	36-37	0.39702850278530377
1103	38-39	0.4057726096212946
1103	40-41	0.4217973687588241
1103	42-43	0.4052457935172171
1103	44-45	0.4287828302983243
1103	46-47	0.41029695205133976
1103	48-49	0.4214107253991628
1103	50-51	0.4222626994132952
1103	52-53	0.39845083258492053
1103	54-55	0.3745801589647897
1103	56-57	0.4020560747897761
1103	58-59	0.3892087184885966
1103	60-61	0.40032018381244683
1103	62-63	0.38990322725573634
1103	64-65	0.38381108012873
1103	66-67	0.4267529494671578
1103	68-69	0.36894761571647905
1103	70-71	0.37593141545852404
1103	72-73	0.4063070757440528
1103	74-75	0.3753211735710522
1103	76-77	0.3976430646574656
1103	78-79	0.3390345975714055
1103	80-81	0.315670507508905
1103	82-83	0.3391830192995684
1103	84-85	0.3554652258424653
1103	86-87	0.3946247351866319
1103	88-89	0.4062814573782134
1103	90-91	0.3630232000400966
1103	92-93	0.42026806881336043
1103	94-95	0.44452589546937915
1103	96-97	0.41547516432965637
1103	98-99	0.44978480357603345
1103	100	0.46077788618946514
1104	1	0.3137923692397635
1104	2	0.32072257888553324
1104	3	0.37460580854521197
1104	4	0.30812642051698447
1104	5	0.24113487101227093
1104	6	0.26685965568237435
1104	7	0.23535755478452813
1104	8	0.25059530829336296
1104	9	0.3026428682145408
1104	10-11	0.30192442476266734
1104	12-13	0.3240906804689061
1104	14-15	0.40824335792950706
1104	16-17	0.3822925425885231
1104	18-19	0.3965426498320852
1104	20-21	0.38913233915213397
1104	22-23	0.39712564598233513
1104	24-25	0.38712434540984475
1104	26-27	0.4310377534494805
1104	28-29	0.4200954385188709
1104	30-31	0.40468579200685184
1104	32-33	0.42875696169049604
1104	34-35	0.40870988250413376
1104	36-37	0.4193743675616304
1104	38-39	0.46192894317294986
1104	40-41	0.4610865230807306
1104	42-43	0.4523349419462548
1104	44-45	0.5036889976998893
1104	46-47	0.5527649630538178
1104	48-49	0.5022997007346319
1104	50-51	0.5299167305201777
1104	52-53	0.538515209576282
1104	54-55	0.49684200550741764
1104	56-57	0.4581387356805422
1104	58-59	0.4551436310477328
1104	60-61	0.4978564831505281
1104	62-63	0.5101048776272208
1104	64-65	0.5059502707215202
1104	66-67	0.4745960238427642
1104	68-69	0.44315358591943976
1104	70-71	0.49192946269033655
1104	72-73	0.48746668980082575
1104	74-75	0.5282968525978333
1104	76-77	0.46179397974486136
1104	78-79	0.46807248739374785
1104	80-81	0.46444301426481616
1104	82-83	0.4593570940684515
1104	84-85	0.46315052149707725
1104	86-87	0.5030619677085966
1104	88-89	0.47664689337186417
1104	90-91	0.45518066914940647
1104	92-93	0.46041979294250623
1104	94-95	0.46861828393727833
1104	96-97	0.4814134530113705
1104	98-99	0.5303381557328599
1104	100	0.5827782780085577
1105	1	0.2623441940554301
1105	2	0.27656914686518164
1105	3	0.3517830119826115
1105	4	0.29334911208941605
1105	5	0.2191459638149098
1105	6	0.24601042898410697
1105	7	0.2019745370904431
1105	8	0.24464477812105656
1105	9	0.26484110636852165
1105	10-11	0.23255291532148448
1105	12-13	0.24960707010382777
1105	14-15	0.29828564382793843
1105	16-17	0.3128054868936516
1105	18-19	0.34506366910627406
1105	20-21	0.31849804025998907
1105	22-23	0.30457842915941313
1105	24-25	0.3088038544738936
1105	26-27	0.32971015348669397
1105	28-29	0.3686176465405282
1105	30-31	0.30617993888603934
1105	32-33	0.3129769776074838
1105	34-35	0.31301882488298105
1105	36-37	0.3856110412496889
1105	38-39	0.3533285975349969
1105	40-41	0.36027431873353777
1105	42-43	0.320098413443894
1105	44-45	0.3609558970017943
1105	46-47	0.35189886202866205
1105	48-49	0.34729814879650434
1105	50-51	0.4084926866829761
1105	52-53	0.3507086768904273
1105	54-55	0.37196508049505184
1105	56-57	0.3936217312480821
1105	58-59	0.38368002795914435
1105	60-61	0.37562323060975444
1105	62-63	0.36396465687069934
1105	64-65	0.39481727030389635
1105	66-67	0.40833694962388734
1105	68-69	0.38026733922495026
1105	70-71	0.3429453164605434
1105	72-73	0.3467376022236053
1105	74-75	0.36531084621600485
1105	76-77	0.3613840701829574
1105	78-79	0.3596567032909377
1105	80-81	0.33480944646368016
1105	82-83	0.36152751372031133
1105	84-85	0.3552824103339205
1105	86-87	0.35706215500991334
1105	88-89	0.32256811514522354
1105	90-91	0.3090922438181849
1105	92-93	0.327825632595367
1105	94-95	0.35564899700552033
1105	96-97	0.40257775892369096
1105	98-99	0.40528986994293703
1105	100	0.3947673600084016
1106	1	0.18286959302599826
1106	2	0.21604866539880518
1106	3	0.34450599157093365
1106	4	0.2535913055295751
1106	5	0.19365240561823782
1106	6	0.21427050251163138
1106	7	0.21666777345237875
1106	8	0.20531089471484165
1106	9	0.23170424736797912
1106	10-11	0.2457450280551967
1106	12-13	0.20606285328214113
1106	14-15	0.3372235265060439
1106	16-17	0.3225684089470846
1106	18-19	0.2683323449772814
1106	20-21	0.24099972645876022
1106	22-23	0.2793977512235344
1106	24-25	0.2820365425371918
1106	26-27	0.2762164375562719
1106	28-29	0.2923571666414091
1106	30-31	0.30141400730600054
1106	32-33	0.3637172266514028
1106	34-35	0.39394637321596804
1106	36-37	0.3989484174695832
1106	38-39	0.38487713425300285
1106	40-41	0.42895900717167734
1106	42-43	0.43868466957658114
1106	44-45	0.3904424278244889
1106	46-47	0.41821676587036904
1106	48-49	0.41483479470520024
1106	50-51	0.4560109268128514
1106	52-53	0.42037897652009093
1106	54-55	0.36770951976648547
1106	56-57	0.4176303196029423
1106	58-59	0.3872295580445666
1106	60-61	0.423038463629247
1106	62-63	0.4555082357411564
1106	64-65	0.4164970275123281
1106	66-67	0.42243303855046577
1106	68-69	0.4331775236024171
1106	70-71	0.5083423678699432
1106	72-73	0.48539238636889337
1106	74-75	0.49154177899252716
1106	76-77	0.49713627050736164
1106	78-79	0.4621171776560047
1106	80-81	0.375172956977579
1106	82-83	0.3696911506229199
1106	84-85	0.3895503413438064
1106	86-87	0.46450260547680244
1106	88-89	0.45528163860187476
1106	90-91	0.4713531858679758
1106	92-93	0.47750622833166645
1106	94-95	0.47255214041505766
1106	96-97	0.5143927501286143
1106	98-99	0.5374948588285626
1106	100	0.5538427556440695
1107	1	0.21086038152453312
1107	2	0.21064287475190113
1107	3	0.292121974212634
1107	4	0.24408009850925794
1107	5	0.2101215842308548
1107	6	0.17390183928455372
1107	7	0.18054780533439185
1107	8	0.20574516719415925
1107	9	0.20778020252151208
1107	10-11	0.19437995492967985
1107	12-13	0.1544636703534863
1107	14-15	0.21797934064269242
1107	16-17	0.21551003615503816
1107	18-19	0.21791325869377687
1107	20-21	0.24816446691529137
1107	22-23	0.25735854344917897
1107	24-25	0.2671361969644366
1107	26-27	0.24687187878244288
1107	28-29	0.24652523410926364
1107	30-31	0.28676140182222554
1107	32-33	0.2919162241953117
1107	34-35	0.28585630994508193
1107	36-37	0.3151159713585585
1107	38-39	0.30788053365914436
1107	40-41	0.2812716776989319
1107	42-43	0.2890527097967279
1107	44-45	0.3644074085340989
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2216	94-95	-1.4977182059087752
2216	96-97	-1.662258735018078
2216	98-99	-1.7419233069279976
2216	100	-1.7494649097170232
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	576.0
3	76.0
4	101.0
5	185.0
6	431.0
7	1295.0
8	3056.0
9	5163.0
10	6939.0
11	9423.0
12	13474.0
13	19174.0
14	23297.0
15	30484.0
16	39445.0
17	55326.0
18	56450.0
19	30911.0
20	22638.0
21	22225.0
22	23283.0
23	25759.0
24	30198.0
25	37322.0
26	47501.0
27	61916.0
28	81410.0
29	107239.0
30	139860.0
31	179567.0
32	231334.0
33	301556.0
34	409417.0
35	592048.0
36	974318.0
37	1781479.0
38	2452781.0
39	973823.0
40	3276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.016890144940895	20.7084065034823	15.023019087098218	23.251684264478587
2	15.38158647728535	20.589367989490995	39.92223536264789	24.106810170575763
3	18.07515751431876	25.199721288458715	30.820241061832753	25.904880135389767
4	12.681770772737384	17.464827502043992	37.90062199761893	31.952779727599694
5	12.769078805160763	37.70798157821815	35.39585533786741	14.127084278753674
6	30.491695425755612	38.17477543918656	17.21077828229128	14.122750852766542
7	26.488421054546595	33.1761335959747	22.096781309225634	18.238664040253077
8	24.944046202077693	36.35503929841828	20.039032350641676	18.661882148862343
9	24.64993303956703	16.86596747649108	21.809043717692358	36.67505576624953
10-11	23.78215495688567	27.25698700452861	28.38216318906403	20.578694849521693
12-13	24.51756328161292	24.63913006326669	28.97237211112601	21.870934543994377
14-15	21.634405195916248	26.615409210469014	26.86316488933799	24.887020704276743
16-17	20.517499291623327	29.99822848979551	27.688528254791834	21.79574396378933
18-19	20.418929189166818	28.25398453351065	30.714041412860116	20.613044864462413
20-21	21.424562546135448	27.364704603516003	30.65633657147509	20.554396278873455
22-23	21.174851240898555	27.15546059492725	30.773150500138946	20.89653766403525
24-25	21.016523937673767	27.276066556024976	30.68479671295031	21.022612793350948
26-27	20.77681290987493	27.37838320926698	30.709481877609797	21.135322003248298
28-29	20.785897069492506	27.388793039293546	30.88157415009279	20.943735741121156
30-31	21.095239305377632	27.66447040959902	30.468340975120856	20.7719493099025
32-33	20.840917162610964	27.44586560091018	30.807880993153365	20.905336243325497
34-35	21.02576808270747	27.309143084810994	30.66957173115434	20.9955171013272
36-37	21.203265900725388	27.4570152941139	30.320960581510164	21.01875822365055
38-39	21.067963681994133	27.55347618512668	30.43004262994903	20.948517502930155
40-41	21.037542144432432	27.399145581753494	30.416273060901293	21.147039212912784
42-43	21.059282372359164	27.478334816793097	30.232601109115475	21.229781701732257
44-45	21.210565705290744	27.61124356377823	29.983418528041028	21.194772202889993
46-47	21.293774381006138	27.755693279040372	29.64323285375967	21.307299486193816
48-49	21.33589607261418	27.767575359680247	29.330546521131456	21.565982046574117
50-51	21.4194174346622	27.880426699728794	29.095070971838215	21.605084893770787
52-53	21.406159534158764	28.00437556198262	29.0153928090785	21.574072094780117
54-55	21.46325037329063	28.162276474753817	28.90029012743503	21.47418302452052
56-57	21.361661426422746	27.97129334799055	28.951115869502235	21.71592935608447
58-59	21.350085391950188	27.693638470267455	29.236202548341396	21.72007358944096
60-61	21.454995454109245	27.914805140699755	29.023119004097442	21.60708040109356
62-63	21.23799113926526	27.956244607582065	29.09506528663217	21.710698966520503
64-65	21.277781896393716	27.790867648858026	29.175755416068395	21.755595038679868
66-67	21.185522372650247	27.78471057070827	29.14317918541458	21.886587871226897
68-69	21.481114511938394	27.96768483217072	28.885805864642855	21.665394791248037
70-71	21.392031797130016	27.781731522739232	28.88668542936268	21.939551250768073
72-73	21.329187529477796	27.889722011616925	28.976443462445122	21.804646996460164
74-75	21.43697903614381	27.998724467171122	28.726493378554217	21.83780311813085
76-77	21.447280629502398	27.94855820900546	28.61331798176095	21.990843179731197
78-79	21.471065960411586	27.951015066383388	28.77704242704024	21.800876546164783
80-81	21.376799791193264	27.91763322501285	28.789126176676945	21.91644080711694
82-83	21.294665321391104	27.917416666131306	28.931069333180666	21.856848679296924
84-85	21.339676734635958	27.84944801197441	28.689795373515764	22.12107987987387
86-87	21.423459616162177	27.950673105655234	28.64838433266369	21.977482945518897
88-89	21.310233797409385	27.821776875775107	28.698407720696128	22.169581606119376
90-91	21.171879748038332	27.800441865860776	28.695362079913465	22.332316306187426
92-93	21.252205091895387	27.908013705752268	28.632927450692897	22.20685375165945
94-95	21.144009590322536	27.71485414111387	28.74386604930993	22.397270219253667
96-97	21.122622453802155	27.80755530952509	28.669688429655604	22.40013380701715
98-99	21.108030044051837	27.780986446179128	28.676582996508433	22.434400513260606
100	21.181405659514915	27.719076859681923	28.580006813212904	22.519510667590257
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	841.0
1	668.5
2	861.5
3	1279.5
4	902.0
5	647.0
6	1755.0
7	4840.5
8	8168.5
9	9517.0
10	7820.0
11	5571.5
12	5371.0
13	5944.5
14	6758.5
15	7586.5
16	8489.5
17	9640.5
18	11159.0
19	12770.0
20	14829.5
21	18309.5
22	24242.0
23	33433.5
24	44318.0
25	56125.5
26	71532.5
27	91500.0
28	117414.0
29	142799.5
30	162445.0
31	182888.0
32	204113.5
33	224233.0
34	244405.5
35	262595.0
36	275248.5
37	285497.0
38	295555.5
39	301688.0
40	306159.0
41	306647.0
42	302081.0
43	300348.0
44	297597.0
45	292685.0
46	288347.5
47	280544.0
48	271054.5
49	267335.0
50	264394.0
51	255551.5
52	242858.5
53	232779.0
54	225941.5
55	215042.0
56	198048.0
57	176450.0
58	157529.5
59	142532.5
60	123837.5
61	102072.5
62	84491.0
63	69479.0
64	55604.5
65	43734.0
66	33761.0
67	25861.0
68	19297.5
69	14371.5
70	10483.0
71	7620.5
72	5610.0
73	3911.5
74	2687.0
75	1778.5
76	1061.0
77	656.0
78	435.5
79	316.0
80	210.5
81	76.5
82	42.0
83	25.0
84	17.0
85	12.5
86	6.5
87	5.5
88	7.5
89	5.0
90	2.0
91	0.5
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.5
98	0.5
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06038825863958023
2	1.4781535724242947E-4
3	0.0
4	0.11913917793739816
5	0.0018988588199604403
6	0.0016487097538578671
7	0.0
8	0.0
9	1.1370412095571496E-4
10-11	0.0
12-13	1.7055618143357246E-5
14-15	3.411123628671449E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.876117995769297E-4
30-31	2.3309344795921573E-4
32-33	1.7055618143357246E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.8192659352914394E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	5.685206047785748E-6
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.2507453305128646E-4
80-81	2.3309344795921573E-4
82-83	3.1268633262821616E-4
84-85	0.0
86-87	0.0
88-89	5.685206047785748E-5
90-91	6.253726652564323E-5
92-93	6.196874592086466E-4
94-95	6.481134894475753E-4
96-97	5.685206047785748E-6
98-99	3.979644233450024E-5
100	9.096329676457197E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8794756.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.904136508706614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48566605570431	36.58497632862384
2	16.325349539446748	16.947063420955892
3	5.680409233832124	8.845102088944202
4	2.5529561680348634	5.3003594178570435
5	1.4129206361412983	3.666821278712326
6	0.8251927342898122	2.5698549795942762
7	0.5412717267941746	1.966596911707973
8	0.37690791031536275	1.565046370257595
9	0.27303251921726	1.275436543389185
>10	1.4069981767621218	12.924706184162577
>50	0.08074936460809443	2.856029299044751
>100	0.03565009295144025	3.3331576198846378
>500	0.002018644201638759	0.7040365042000234
>1k	7.894779584429263E-4	0.6395397584983745
>5k	2.192993561058767E-5	0.08164698607193342
>10k+	6.578980683176301E-5	0.7396263080953802
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25796	0.29331115041736233	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24595	0.27965528549058094	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14588	0.165871571650197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.2740824191142996E-5	0.0	0.0	0.0
6	0.0	2.2740824191142996E-5	0.0	0.0	0.0
7	0.0	2.2740824191142996E-5	0.0	1.1370412095571498E-5	0.0
8	0.0	2.2740824191142996E-5	0.0	1.1370412095571498E-5	0.0
9	4.548164838228599E-5	2.2740824191142996E-5	0.0	2.2740824191142996E-5	0.0
10-11	4.548164838228599E-5	3.411123628671449E-5	0.0	5.116685443007174E-5	0.0
12-13	4.548164838228599E-5	3.411123628671449E-5	0.0	9.096329676457199E-5	0.0
14-15	4.548164838228599E-5	4.548164838228599E-5	0.0	1.0233370886014347E-4	0.0
16-17	4.548164838228599E-5	6.253726652564323E-5	0.0	1.1370412095571497E-4	0.0
18-19	4.548164838228599E-5	7.959288466900048E-5	0.0	1.1370412095571497E-4	0.0
20-21	4.548164838228599E-5	7.959288466900048E-5	0.0	1.1938932700350071E-4	0.0
22-23	4.548164838228599E-5	7.959288466900048E-5	0.0	2.2740824191142992E-4	0.0
24-25	4.548164838228599E-5	7.959288466900048E-5	0.0	4.548164838228599E-4	0.0
26-27	4.548164838228599E-5	1.0233370886014347E-4	0.0	9.03947761597934E-4	0.0
28-29	4.548164838228599E-5	1.0233370886014347E-4	0.0	0.002933566320657446	0.0
30-31	4.548164838228599E-5	1.1370412095571497E-4	0.0	0.009056533234122697	0.0
32-33	4.548164838228599E-5	1.1370412095571497E-4	0.0	0.02206428467145649	0.0
34-35	5.685206047785749E-5	1.1370412095571497E-4	0.0	0.04307680622407262	0.0
36-37	5.685206047785749E-5	1.3075973909907221E-4	0.0	0.07827391686591419	0.0
38-39	5.685206047785749E-5	1.3644494514685796E-4	0.0	0.12543270103229698	0.0
40-41	5.685206047785749E-5	1.4781535724242947E-4	0.0	0.17358639625704225	0.0
42-43	5.685206047785749E-5	1.4781535724242947E-4	0.0	0.222126685493037	0.0
44-45	5.685206047785749E-5	1.4781535724242947E-4	0.0	0.28212266491532	0.0
46-47	5.685206047785749E-5	1.6487097538578672E-4	0.0	0.3561952145119205	0.0
48-49	5.685206047785749E-5	1.8192659352914397E-4	0.0	0.4338835551549127	0.0
50-51	5.685206047785749E-5	1.9329700562471544E-4	0.0	0.5112023574047989	0.0
52-53	5.685206047785749E-5	2.0466741772028695E-4	0.0	0.5888054199570745	1.1370412095571498E-5
54-55	5.685206047785749E-5	2.0466741772028695E-4	0.0	0.6767953539586544	1.1370412095571498E-5
56-57	5.685206047785749E-5	2.0466741772028695E-4	0.0	0.7833531709123027	1.1370412095571498E-5
58-59	5.685206047785749E-5	2.1603782981585845E-4	0.0	0.8997748203588594	1.1370412095571498E-5
60-61	5.685206047785749E-5	2.1603782981585845E-4	0.0	1.0192209994228378	1.1370412095571498E-5
62-63	6.822247257342898E-5	2.2740824191142995E-4	0.0	1.1395256446000321	1.1370412095571498E-5
64-65	6.822247257342898E-5	2.2740824191142995E-4	0.0	1.2710813125457943	1.1370412095571498E-5
66-67	6.822247257342898E-5	2.2740824191142995E-4	0.0	1.4224556087741376	1.1370412095571498E-5
68-69	6.822247257342898E-5	2.330934479592157E-4	0.0	1.5892424985980282	1.1370412095571498E-5
70-71	6.822247257342898E-5	2.501490661025729E-4	0.0	1.7673941153114425	1.1370412095571498E-5
72-73	6.822247257342898E-5	2.6151947819814443E-4	0.0	1.9515663652294617	1.1370412095571498E-5
74-75	7.959288466900048E-5	2.6720468424593015E-4	0.0	2.1402640391615186	1.1370412095571498E-5
76-77	7.959288466900048E-5	2.7288989029371593E-4	0.0	2.344476640397983	1.1370412095571498E-5
78-79	7.959288466900048E-5	2.785750963415017E-4	0.0	2.575421080471135	1.1370412095571498E-5
80-81	7.959288466900048E-5	2.9563071448485893E-4	0.0	2.825365479155988	1.1370412095571498E-5
82-83	7.959288466900048E-5	2.9563071448485893E-4	0.0	3.081825123971603	1.1370412095571498E-5
84-85	7.959288466900048E-5	3.0700112658043043E-4	0.0	3.341383206083261	1.1370412095571498E-5
86-87	7.959288466900048E-5	3.1268633262821616E-4	0.0	3.6160753066941256	1.1370412095571498E-5
88	7.959288466900048E-5	3.1837153867600193E-4	0.0	3.84188032050008	1.1370412095571498E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15900	0.0	57.41007	1
GTATCAA	28650	0.0	40.553463	1
ATCAACG	35835	0.0	32.19534	3
TCAACGC	35925	0.0	32.140877	4
TATCAAC	37250	0.0	31.022875	2
CAACGCA	37385	0.0	30.963701	5
GTGGTAT	6240	0.0	30.328354	1
AACGCAG	39720	0.0	29.202118	6
TGGTATC	7060	0.0	25.925362	2
CGCAGAG	45170	0.0	25.418177	8
ACGCAGA	45455	0.0	25.372538	7
GCAGAGT	48860	0.0	23.104181	9
GTACATG	25140	0.0	22.84557	1
GAGTACT	34150	0.0	21.675087	12-13
TACATGG	25975	0.0	21.538044	2
CAGAGTA	47655	0.0	20.710083	10-11
ACATGGG	26410	0.0	20.36375	3
AGAGTAC	45835	0.0	20.322514	10-11
GTACTTT	37365	0.0	19.62143	14-15
AGTACTT	35940	0.0	19.425205	12-13
>>END_MODULE