FastQCFastQC Report
Wed 25 May 2016
SRR2049530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978313
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60650.044.640911
GTATCAA93400.032.385361
ATCAACG96600.030.0885523
TCAACGC97050.029.8963134
CAACGCA104550.027.8492955
TATCAAC108250.027.1340162
AACGCAG118400.024.6343386
GTACATG157550.024.5265621
TACATGG159650.023.2380222
TACCTGG41000.021.5919742
ACATGGG163000.021.567473
GTGGTAT37050.020.8591611
ACGCAGA141650.020.4097337
CGCAGAG138950.020.3494038
GTACCTG47650.019.4412331
TGGTATC41300.017.8419592
CATGGGG109700.017.6325554
GCAGAGT157950.016.9277049
GTACGAC3109.506439E-716.507013
CAGAGTA152100.015.764379510-11
Produced by FastQC (version 0.11.5)