##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049530_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5978313 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.63059946844536 31.0 31.0 34.0 27.0 34.0 2 30.807076009569922 31.0 31.0 34.0 27.0 34.0 3 30.895139481656447 31.0 31.0 34.0 27.0 34.0 4 34.53411539342286 37.0 35.0 37.0 32.0 37.0 5 34.573528184288776 37.0 35.0 37.0 32.0 37.0 6 34.65737156953809 37.0 35.0 37.0 32.0 37.0 7 30.744084995215207 37.0 33.0 37.0 2.0 37.0 8 32.489978025573436 37.0 33.0 37.0 17.0 37.0 9 35.28803493560809 39.0 34.0 39.0 28.0 39.0 10-11 35.81867593750946 39.0 36.0 39.0 30.0 39.0 12-13 35.975387370985764 39.0 37.0 39.0 31.0 39.0 14-15 37.16274147907612 40.0 37.0 41.0 31.0 41.0 16-17 37.174307869126295 40.0 37.0 41.0 31.0 41.0 18-19 37.16929341103418 40.0 37.0 41.0 31.0 41.0 20-21 37.24413567840961 40.0 37.0 41.0 31.0 41.0 22-23 37.20955501995295 40.0 37.0 41.0 31.0 41.0 24-25 37.18559541797159 40.0 37.0 41.0 31.0 41.0 26-27 37.21107267217357 40.0 37.0 41.0 31.0 41.0 28-29 37.1984527240377 40.0 37.0 41.0 31.0 41.0 30-31 37.12615883444042 40.0 37.0 41.0 31.0 41.0 32-33 37.078023097820406 40.0 37.0 41.0 30.0 41.0 34-35 37.025845334628684 40.0 37.0 41.0 30.0 41.0 36-37 36.857246935715814 40.0 37.0 41.0 30.0 41.0 38-39 36.74617103520676 40.0 37.0 41.0 30.0 41.0 40-41 36.67486580244293 40.0 36.5 41.0 30.0 41.0 42-43 36.56143422065723 40.0 36.0 41.0 30.0 41.0 44-45 36.44639599833599 40.0 36.0 41.0 30.0 41.0 46-47 36.289195212763204 40.0 36.0 41.0 29.5 41.0 48-49 36.20993999477779 40.0 36.0 41.0 29.0 41.0 50-51 36.07447769964537 39.5 35.0 41.0 29.0 41.0 52-53 35.95131904267976 39.0 35.0 41.0 29.0 41.0 54-55 35.827741036643616 39.0 35.0 41.0 28.0 41.0 56-57 35.631520714957546 39.0 35.0 41.0 28.0 41.0 58-59 35.448742479692854 39.0 35.0 41.0 28.0 41.0 60-61 35.221389964024965 38.5 34.5 40.0 28.0 41.0 62-63 34.972608827941926 38.0 34.0 40.0 27.0 41.0 64-65 34.60496004474841 37.5 34.0 40.0 26.5 41.0 66-67 31.121918842322238 36.0 30.0 40.0 6.5 41.0 68-69 31.468699196579372 36.0 30.0 39.0 11.0 41.0 70-71 31.622834485246926 35.5 30.0 39.0 15.5 41.0 72-73 32.84234766563745 35.0 31.5 39.0 23.5 40.5 74-75 32.92215496244509 35.0 33.0 38.5 25.0 40.0 76-77 32.624662091128386 35.0 33.0 37.5 24.5 39.5 78-79 29.268274260648447 35.0 29.0 37.0 6.5 39.0 80-81 31.041408838914926 35.0 30.0 36.0 19.0 39.0 82-83 31.439986916041363 35.0 32.0 36.0 21.0 37.5 84-85 29.610441524222637 35.0 30.0 35.5 11.0 37.0 86-87 29.242025969533547 34.0 28.0 35.0 10.5 37.0 88-89 30.461821587461213 34.0 30.5 35.0 18.0 36.0 90-91 30.602124127659422 35.0 31.5 35.0 17.5 36.0 92-93 30.44599989997178 35.0 32.0 35.0 10.0 36.0 94-95 30.229317283989644 35.0 32.0 35.0 4.5 36.0 96-97 30.028972052818247 35.0 31.5 35.0 2.0 35.0 98-99 29.826125447095194 35.0 31.0 35.0 2.0 35.0 100 29.602246486592456 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3032183002218538 1101 2 0.3318207115403382 1101 3 0.306609573437882 1101 4 0.387817242536741 1101 5 0.36983584625356514 1101 6 0.3925754358245328 1101 7 -12.054033768751289 1101 8 -6.484479325557903 1101 9 -2.1072156939023188 1101 10-11 -0.040813675937428684 1101 12-13 0.43489130002081566 1101 14-15 0.6114450129718918 1101 16-17 0.6285634705603584 1101 18-19 0.6924061496775664 1101 20-21 0.6212966565476066 1101 22-23 0.5773311855605243 1101 24-25 0.6313307554566094 1101 26-27 0.5373869192600367 1101 28-29 0.5373408935959603 1101 30-31 0.6119512617799643 1101 32-33 0.6031524931977899 1101 34-35 0.6244775460000582 1101 36-37 0.6745754084493072 1101 38-39 0.6630225737836355 1101 40-41 0.6698421282219158 1101 42-43 0.5943783758916936 1101 44-45 0.559553651803931 1101 46-47 0.5895036142848014 1101 48-49 0.5140908871147261 1101 50-51 0.6223033259449195 1101 52-53 0.6330257116219826 1101 54-55 0.5743513807068084 1101 56-57 0.5114917327370421 1101 58-59 0.5166011589598511 1101 60-61 0.5087365765460916 1101 62-63 0.4852613420627705 1101 64-65 0.33848942722323727 1101 66-67 -10.2346278083647 1101 68-69 -8.113901987233767 1101 70-71 -6.58955280398466 1101 72-73 -1.5027836473147786 1101 74-75 -0.22016118436231125 1101 76-77 0.053570524689092736 1101 78-79 3.2011076199860398 1101 80-81 1.0449403369035153 1101 82-83 0.3611213246636247 1101 84-85 -5.366697887824085 1101 86-87 -5.8397314456610445 1101 88-89 -1.297464204409053 1101 90-91 -0.006377681230144816 1101 92-93 0.3717893273810269 1101 94-95 0.4215029169450446 1101 96-97 0.5087295338034572 1101 98-99 0.5432095197797864 1101 100 0.5945792588978556 1102 1 0.22111373459899752 1102 2 0.22911979435709995 1102 3 0.1925377361299354 1102 4 0.27706295924208746 1102 5 0.18719301765887764 1102 6 0.2875918929810979 1102 7 -12.175243496844516 1102 8 -6.596831327289664 1102 9 -2.3158981447276332 1102 10-11 -0.24796575275970412 1102 12-13 0.2980326061690377 1102 14-15 0.4439735540369938 1102 16-17 0.48633443070722393 1102 18-19 0.5012469034847697 1102 20-21 0.46559636098092483 1102 22-23 0.4727167374497796 1102 24-25 0.5440737754036675 1102 26-27 0.4711438579400351 1102 28-29 0.45908681961411446 1102 30-31 0.49643928466633724 1102 32-33 0.4883232344151267 1102 34-35 0.530498247850467 1102 36-37 0.6425957522430465 1102 38-39 0.631456438179633 1102 40-41 0.5355556850186645 1102 42-43 0.5176120645196676 1102 44-45 0.47814436184049924 1102 46-47 0.5475161849194166 1102 48-49 0.43566923328058493 1102 50-51 0.532925575843187 1102 52-53 0.4501302243946128 1102 54-55 0.41991919530626376 1102 56-57 0.42104544326775084 1102 58-59 0.3874150143934898 1102 60-61 0.32914033296145107 1102 62-63 0.39052977785540577 1102 64-65 0.2788974802034119 1102 66-67 -10.211468321739389 1102 68-69 -6.630729865804746 1102 70-71 -6.178572310210349 1102 72-73 -1.0646485067860318 1102 74-75 -0.12791910332036593 1102 76-77 0.016885564068559233 1102 78-79 3.205427528181609 1102 80-81 1.0759908516085446 1102 82-83 0.39193456778349756 1102 84-85 -5.387415722896886 1102 86-87 -5.8459587573262475 1102 88-89 -1.3445951270290983 1102 90-91 -0.017361304256144905 1102 92-93 0.2983245061462618 1102 94-95 0.2989775998876212 1102 96-97 0.3756115773738742 1102 98-99 0.3921475226075444 1102 100 0.49358946492415967 1103 1 0.30578854788343435 1103 2 0.3454284661751963 1103 3 0.33763418742093876 1103 4 0.34645502514623416 1103 5 0.3552468226300789 1103 6 0.40796499219674587 1103 7 -12.096679397436567 1103 8 -6.5128238348164125 1103 9 -2.139918508269865 1103 10-11 -0.09389532931538014 1103 12-13 0.4167863974802586 1103 14-15 0.6111197585060211 1103 16-17 0.6531462033738222 1103 18-19 0.6799742137849449 1103 20-21 0.6419857759485623 1103 22-23 0.6507748922652112 1103 24-25 0.7394314216642641 1103 26-27 0.6568396754013577 1103 28-29 0.6636846116345154 1103 30-31 0.6635409566043506 1103 32-33 0.6880276184676717 1103 34-35 0.6604427556467414 1103 36-37 0.7158231406836322 1103 38-39 0.7181161597729258 1103 40-41 0.7182977405902591 1103 42-43 0.7320844449268833 1103 44-45 0.6748551215504719 1103 46-47 0.7240890463947238 1103 48-49 0.635308319633161 1103 50-51 0.6476961449105545 1103 52-53 0.58283320558904 1103 54-55 0.5681535429972513 1103 56-57 0.5992139703768089 1103 58-59 0.5944229003208292 1103 60-61 0.47660739098066784 1103 62-63 0.3997416946606336 1103 64-65 0.3346503743592635 1103 66-67 -10.236793175711881 1103 68-69 -8.158149374922825 1103 70-71 -6.624966067516727 1103 72-73 -1.3781319856339955 1103 74-75 -0.1750743001916817 1103 76-77 0.09627655919049971 1103 78-79 3.253286781032479 1103 80-81 1.104794872112187 1103 82-83 0.4147724174993819 1103 84-85 -5.411569346285436 1103 86-87 -5.886085507417189 1103 88-89 -1.31060089841176 1103 90-91 -0.07086142776303817 1103 92-93 0.35926477301120485 1103 94-95 0.4887165166663259 1103 96-97 0.5732469083164631 1103 98-99 0.5990240834352321 1103 100 0.6896602779864338 1104 1 0.15367691277621987 1104 2 0.1985055469837853 1104 3 0.19614141706320254 1104 4 0.19612233598706297 1104 5 0.25831169145558164 1104 6 0.2507773875396282 1104 7 -12.26298960765002 1104 8 -6.669334219874113 1104 9 -2.361554774194957 1104 10-11 -0.32354167113270194 1104 12-13 0.16991448688425947 1104 14-15 0.4228488439757072 1104 16-17 0.4348452757503196 1104 18-19 0.41248208378844 1104 20-21 0.40639250022867657 1104 22-23 0.41955531763198195 1104 24-25 0.526595859564587 1104 26-27 0.530116175881254 1104 28-29 0.4806433226337603 1104 30-31 0.43862037686031385 1104 32-33 0.44277284239603887 1104 34-35 0.4425440132162137 1104 36-37 0.4506801492710011 1104 38-39 0.4914889739285755 1104 40-41 0.4509957277631287 1104 42-43 0.4314223764478413 1104 44-45 0.41043539622158676 1104 46-47 0.37963727440738637 1104 48-49 0.3454206253932597 1104 50-51 0.397504465497029 1104 52-53 0.4038442190395912 1104 54-55 0.35052596908086286 1104 56-57 0.37522128422138223 1104 58-59 0.340361436234339 1104 60-61 0.34154643274353447 1104 62-63 0.2528212115109483 1104 64-65 0.20130817595234873 1104 66-67 -10.43699007672788 1104 68-69 -8.385975199210499 1104 70-71 -6.779869936014535 1104 72-73 -1.6793754472450502 1104 74-75 -0.4551961679451182 1104 76-77 -0.18251025178304303 1104 78-79 2.9725215599553394 1104 80-81 0.9110347876938079 1104 82-83 0.22855087602725277 1104 84-85 -5.559126536601514 1104 86-87 -6.067602363872691 1104 88-89 -1.5134155116117576 1104 90-91 -0.19075672880801164 1104 92-93 0.17090499591537878 1104 94-95 0.22681287821559337 1104 96-97 0.2723108910582681 1104 98-99 0.2549861737539132 1104 100 0.3578623923435522 1105 1 0.11090004733020464 1105 2 0.17285229537664648 1105 3 0.15483085698966903 1105 4 0.2069558422401343 1105 5 0.23705700134532748 1105 6 0.2648814579352532 1105 7 -12.135530769978697 1105 8 -6.630777288480832 1105 9 -2.473603727233474 1105 10-11 -0.38268708724649514 1105 12-13 0.1854558850862702 1105 14-15 0.3476102147321214 1105 16-17 0.3624138735522351 1105 18-19 0.37693456382228874 1105 20-21 0.40611323653444487 1105 22-23 0.3947775735375316 1105 24-25 0.4006601172160771 1105 26-27 0.34457831679247164 1105 28-29 0.3901284247198973 1105 30-31 0.40420987174283596 1105 32-33 0.40238741138413303 1105 34-35 0.39698289507123263 1105 36-37 0.49643570080527155 1105 38-39 0.4787271423680721 1105 40-41 0.44595694949784104 1105 42-43 0.3926928714900413 1105 44-45 0.2942272252369307 1105 46-47 0.33802006886605795 1105 48-49 0.34230632226927327 1105 50-51 0.39241880132716034 1105 52-53 0.38404296664134563 1105 54-55 0.355705050439326 1105 56-57 0.29546610615322777 1105 58-59 0.2790999485544603 1105 60-61 0.2325210506246762 1105 62-63 0.17520181178021232 1105 64-65 0.09136526378396326 1105 66-67 -10.30604813054591 1105 68-69 -8.385799358070784 1105 70-71 -6.816633601875232 1105 72-73 -1.7501421338650331 1105 74-75 -0.5277455574708867 1105 76-77 -0.2676588540106266 1105 78-79 2.9589960783400855 1105 80-81 0.8369298550636088 1105 82-83 0.14660219245984507 1105 84-85 -5.517314872340865 1105 86-87 -5.976559055988279 1105 88-89 -1.4460193161432855 1105 90-91 -0.15700242371436346 1105 92-93 0.12618625359038305 1105 94-95 0.20780394947771086 1105 96-97 0.3102759607300669 1105 98-99 0.3092394978657609 1105 100 0.3883445121720719 1106 1 0.14588809502603794 1106 2 0.18170137037223455 1106 3 0.14638157003358288 1106 4 0.18124165663455472 1106 5 0.17192028997693143 1106 6 0.19380776623226126 1106 7 -12.27625655357113 1106 8 -6.69225016194703 1106 9 -2.5209873694265212 1106 10-11 -0.44937748005055056 1106 12-13 0.13139513305170425 1106 14-15 0.3177814208597667 1106 16-17 0.30625881090893614 1106 18-19 0.34768498034198103 1106 20-21 0.34630624498767304 1106 22-23 0.3139393886815256 1106 24-25 0.43311144976414084 1106 26-27 0.37661785388083757 1106 28-29 0.33940652287051876 1106 30-31 0.3375800081471283 1106 32-33 0.3441092990608112 1106 34-35 0.39036109809978115 1106 36-37 0.47637317167251325 1106 38-39 0.44138987243811556 1106 40-41 0.4222088313164818 1106 42-43 0.37424661985231467 1106 44-45 0.2829395613061152 1106 46-47 0.37279582143604273 1106 48-49 0.30347432536837005 1106 50-51 0.34737355500576683 1106 52-53 0.34555721649647353 1106 54-55 0.2930246438720161 1106 56-57 0.3028087420221013 1106 58-59 0.259994076177982 1106 60-61 0.31294488106258456 1106 62-63 0.23390914016465558 1106 64-65 0.08440558588201696 1106 66-67 -10.429219656346511 1106 68-69 -8.364050770615826 1106 70-71 -6.839150172115097 1106 72-73 -1.7164759850265625 1106 74-75 -0.5036375119481917 1106 76-77 -0.19010834017178269 1106 78-79 2.9347143189105154 1106 80-81 0.8284869655035294 1106 82-83 0.18168275634404552 1106 84-85 -5.589030961024125 1106 86-87 -6.061596196316831 1106 88-89 -1.5216265963405675 1106 90-91 -0.25749724753204717 1106 92-93 0.09378098229354848 1106 94-95 0.1534135839167199 1106 96-97 0.22017838174092574 1106 98-99 0.2058315951723202 1106 100 0.2689732364407611 1107 1 0.1636924081580311 1107 2 0.18726942900064003 1107 3 0.14761394530059135 1107 4 0.20937321243169293 1107 5 0.21055864470390873 1107 6 0.1969034585244387 1107 7 -12.14451000326692 1107 8 -6.602388040913169 1107 9 -2.362789872719219 1107 10-11 -0.38021272202965406 1107 12-13 0.2217049152368915 1107 14-15 0.33887953263739234 1107 16-17 0.37508277968634474 1107 18-19 0.4149973675612557 1107 20-21 0.36646021215692315 1107 22-23 0.447678951451401 1107 24-25 0.48142559764229276 1107 26-27 0.3979032093473265 1107 28-29 0.46609677735577293 1107 30-31 0.4632588769364503 1107 32-33 0.45023309160999503 1107 34-35 0.4149504464048377 1107 36-37 0.5332899649986373 1107 38-39 0.5679479724621075 1107 40-41 0.5454388854588643 1107 42-43 0.5757682308728533 1107 44-45 0.4716717575182088 1107 46-47 0.5429305390086725 1107 48-49 0.5326291346209331 1107 50-51 0.5296226503726089 1107 52-53 0.513757603866253 1107 54-55 0.4582382640204443 1107 56-57 0.4448266459852661 1107 58-59 0.3925074709177565 1107 60-61 0.4544346565974351 1107 62-63 0.3420694913338309 1107 64-65 0.2585476956053441 1107 66-67 -10.227679340056277 1107 68-69 -8.144965890184395 1107 70-71 -6.660577050195798 1107 72-73 -1.6562418533025038 1107 74-75 -0.40356917743198295 1107 76-77 -0.1342163855338896 1107 78-79 3.015520224533013 1107 80-81 0.898608891393323 1107 82-83 0.2073254250051768 1107 84-85 -5.497470766773898 1107 86-87 -5.917420537432957 1107 88-89 -1.4852026136971475 1107 90-91 -0.22181625921005477 1107 92-93 0.13821302871069463 1107 94-95 0.198896933337938 1107 96-97 0.23614031533533364 1107 98-99 0.31502357717935325 1107 100 0.3782315642607941 1108 1 0.03405913850808773 1108 2 0.08405437797318527 1108 3 0.07117643379924488 1108 4 0.0443843946203728 1108 5 -0.034488471425987655 1108 6 0.06636995478262975 1108 7 -12.389838658001938 1108 8 -6.804557838514004 1108 9 -2.7146511716576924 1108 10-11 -0.5762967719804237 1108 12-13 0.01337170336610427 1108 14-15 0.16400490164637915 1108 16-17 0.1471069578117863 1108 18-19 0.2135091020690396 1108 20-21 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3.285211392804186 2216 8 1.4458464977562855 2216 9 0.14116180072897322 2216 10-11 -0.5746264030084305 2216 12-13 -0.9029964744765735 2216 14-15 -1.0672799118976215 2216 16-17 -1.3037979168407716 2216 18-19 -1.7358628715543603 2216 20-21 -1.3000616722624585 2216 22-23 -1.1060758687447745 2216 24-25 -1.1244866475150772 2216 26-27 -1.5969557603571403 2216 28-29 -1.6876102559403634 2216 30-31 -1.6546546984894093 2216 32-33 -2.5381667695065815 2216 34-35 -1.6319733567352515 2216 36-37 -1.3268560963840415 2216 38-39 -1.589405186103697 2216 40-41 -1.5901944847539085 2216 42-43 -1.23942541326673 2216 44-45 -1.3231398857925925 2216 46-47 -1.5607918496409496 2216 48-49 -1.6615822595713468 2216 50-51 -2.1340554860387684 2216 52-53 -1.8953934685087575 2216 54-55 -1.5986465287218365 2216 56-57 -1.561017906939398 2216 58-59 -1.8845537120684526 2216 60-61 -2.012389787115829 2216 62-63 -1.8621812060854381 2216 64-65 -1.5308136895872408 2216 66-67 1.8143021225573364 2216 68-69 0.9937642145909393 2216 70-71 0.6530286732417494 2216 72-73 -1.130142924812688 2216 74-75 -2.0095742675036163 2216 76-77 -1.6130622763727693 2216 78-79 0.9976299652716776 2216 80-81 -0.4946166038344515 2216 82-83 -1.3700167406787145 2216 84-85 0.4335299281059726 2216 86-87 0.36736109540496287 2216 88-89 -0.8302211620783986 2216 90-91 -1.1276309243900862 2216 92-93 -1.1996272995417705 2216 94-95 -1.1960641751365806 2216 96-97 -1.1966716843941825 2216 98-99 -1.216169207245521 2216 100 -2.373957653266835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89267.0 3 16763.0 4 10117.0 5 9088.0 6 8690.0 7 9529.0 8 10160.0 9 11333.0 10 12637.0 11 14785.0 12 16400.0 13 18566.0 14 21490.0 15 23295.0 16 24455.0 17 25306.0 18 23759.0 19 22373.0 20 22488.0 21 22496.0 22 24231.0 23 28207.0 24 33375.0 25 41829.0 26 51719.0 27 63008.0 28 77541.0 29 99125.0 30 122563.0 31 149908.0 32 199350.0 33 281957.0 34 426850.0 35 583805.0 36 648146.0 37 1093166.0 38 1234507.0 39 405151.0 40 878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52042762497491 20.61871779428495 14.123301880479156 22.73755270026099 2 15.815974484503705 20.785052819666753 40.102800672647206 23.29617202318234 3 18.228508639065172 25.55929819607775 30.714601979742906 25.497591185114178 4 12.718493861028405 17.72811214077816 38.8489511893212 30.704442808872233 5 12.841332845116582 37.53708253243985 35.19223701908545 14.429347603358122 6 29.738857670212234 38.216477829591135 18.361929187643995 13.68273531255264 7 26.415415959497086 32.8562134027326 22.816565239740182 17.911805398030133 8 24.29441621648385 36.182420723441666 21.242885951753607 18.280277108320877 9 24.206661645183182 17.921393543630117 22.341252457005847 35.53069235418086 10-11 23.182819300361153 27.462864523821352 29.255828525538895 20.0984876502786 12-13 23.738887542355176 25.128635787386845 29.121618423123714 22.010858247134266 14-15 21.399757757748716 27.633447763608228 26.33078094104474 24.636013537598316 16-17 20.77928573280509 30.74025286473819 26.969846753965804 21.510614648490918 18-19 20.67920331371074 29.65048835683244 28.87255986764159 20.79774846181523 20-21 21.973807661124468 29.08199185957644 28.235482150232016 20.70871832906708 22-23 21.629565199209644 29.01167344463843 28.308080248397854 21.05068110775407 24-25 21.398018136554576 28.977070956304896 28.348064077608516 21.27684682953201 26-27 21.156059124819983 28.88904984133035 28.387703430481046 21.567187603368616 28-29 21.2182851583716 28.937528028391956 28.520964024466434 21.32322278877001 30-31 21.60760903619466 29.448123575998782 27.97662986196942 20.967637525837137 32-33 21.099597829688744 29.1174784592242 28.51692442332812 21.265999287758937 34-35 21.26010172438336 28.679095149114858 28.544625731247503 21.516177395254278 36-37 21.728641797216337 29.056270398734625 28.00114815018018 21.213939653868852 38-39 21.46262009555372 29.13194149686889 28.25172655327068 21.15371185430671 40-41 21.24816817051901 28.889537901411316 28.423302694255053 21.438991233814622 42-43 21.135371032950783 28.959890002358524 28.340250003763607 21.564488960927083 44-45 21.463420672242776 29.09161573734613 28.217914402610845 21.227049187800247 46-47 21.346454117304052 29.37084185415114 27.988154728544746 21.29454930000006 48-49 21.22042603273554 28.949221623033942 28.354635193140226 21.47571715109029 50-51 21.23275343526211 28.805995090236475 28.328977574196777 21.632273900304636 52-53 21.25685197623703 28.94089258080086 28.220728875221667 21.581526567740443 54-55 21.366474131152195 29.371838875022977 28.04862627128723 21.213060722537595 56-57 21.157659840131224 29.084163995091846 28.01720680694766 21.74096935782927 58-59 21.020652294730546 28.700392729063445 28.40327621866069 21.875678757545316 60-61 21.385640668619196 29.081587847642552 27.95912369394893 21.573647789789323 62-63 21.23102688643427 29.075819020339605 28.057220248325425 21.635933844900695 64-65 21.178361462103336 28.839432205124226 28.24059480277717 21.74161152999527 66-67 21.099610271973326 28.853812172912857 28.07188808176556 21.974689473348256 68-69 21.6550006346404 29.03167143052368 27.811640498990986 21.501687435844932 70-71 21.40863871291232 28.664155576623024 28.042006323865376 21.88519938659928 72-73 21.061847471112486 28.822152504465574 28.40375117624669 21.71224884817525 74-75 21.11034293908935 28.858376308125116 28.204734032472096 21.826546720313438 76-77 21.16712592522582 28.864628364405252 27.99951320962557 21.96873250074336 78-79 21.289881194362074 28.932922478644883 28.114242760162632 21.662953566830403 80-81 21.072330121083127 28.720840689123396 28.238221124270563 21.96860806552291 82-83 20.954458012713282 28.726095684175306 28.391753094680496 21.927693208430913 84-85 21.089948474704595 28.672639924318062 28.144478568946578 22.092933032030768 86-87 21.19674893143235 28.691668718602042 28.144462718564572 21.967119631401037 88-89 20.981272396449942 28.562098254368053 28.279998645078813 22.176630704103196 90-91 20.84698383618761 28.477392592671897 28.248674705889336 22.426948865251155 92-93 21.035408582799388 28.629346072307943 28.083243052185026 22.25200229270764 94-95 20.997806719486174 28.38008632105736 28.261284672053 22.36082228740347 96-97 20.90938440410096 28.351134388627848 28.349662351478976 22.389818855792214 98-99 20.945105756103256 28.32228430473087 28.247058180974783 22.485551758191086 100 21.098791575505242 28.392999003504375 28.014073054152856 22.494136366837523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4359.0 1 3351.0 2 2859.5 3 3304.5 4 2910.5 5 2421.0 6 2213.0 7 2116.0 8 2159.5 9 2194.0 10 1893.0 11 1664.0 12 1671.0 13 1705.5 14 1754.0 15 1950.5 16 2242.0 17 2704.0 18 3442.0 19 4481.5 20 6286.0 21 8954.5 22 13121.0 23 19571.5 24 27177.5 25 36257.0 26 49238.5 27 65421.5 28 85352.0 29 104810.5 30 119678.5 31 134752.0 32 149804.5 33 164222.5 34 177604.0 35 188694.5 36 197606.5 37 204469.0 38 210654.0 39 213894.5 40 214869.0 41 213253.5 42 208408.5 43 204914.5 44 201428.5 45 197887.5 46 194527.5 47 190335.0 48 185946.0 49 183022.0 50 178739.5 51 169673.0 52 159664.0 53 151241.5 54 145563.0 55 138639.5 56 126920.0 57 114447.0 58 103258.0 59 92335.0 60 79737.5 61 65549.5 62 53286.0 63 42693.5 64 33453.0 65 25941.5 66 19822.0 67 14993.0 68 11111.0 69 8198.0 70 5984.5 71 4460.5 72 3396.0 73 2394.5 74 1614.0 75 1097.0 76 733.0 77 490.0 78 352.0 79 274.5 80 197.0 81 119.0 82 88.0 83 82.0 84 60.5 85 50.0 86 40.5 87 28.0 88 29.0 89 25.0 90 16.5 91 16.5 92 16.5 93 8.5 94 6.0 95 8.5 96 9.5 97 10.5 98 9.5 99 14.0 100 24.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018617292202666537 2 0.002375252015075156 3 0.0010370818657370398 4 0.0018901653359400888 5 0.0015723499254722863 6 0.0038806934330805364 7 11.584706254088738 8 1.6727126866726452E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.010882836010761E-4 18-19 0.0 20-21 0.0 22-23 8.363563433363226E-6 24-25 0.0 26-27 1.923619589673542E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.3454253733452904E-5 36-37 1.6727126866726452E-5 38-39 8.363563433363226E-6 40-41 0.0 42-43 5.018138060017935E-5 44-45 1.923619589673542E-4 46-47 0.001045445429170403 48-49 6.69085074669058E-4 50-51 3.5126966420125544E-4 52-53 3.2617897390116576E-4 54-55 3.679967910679819E-4 56-57 0.001630894869505829 58-59 3.094518470344393E-4 60-61 0.0013716244030715689 62-63 0.0025007054665756043 64-65 0.001045445429170403 66-67 5.792746214525737 68-69 5.4447717943172265 70-71 0.002768339496443227 72-73 0.0013632608396382058 74-75 0.003278516865878384 76-77 0.006364671772789415 78-79 5.404701961907983 80-81 0.0052272271458520154 82-83 0.005235590709285379 84-85 5.792972030738437 86-87 0.0036297865300796398 88-89 0.0017647118844396404 90-91 0.003445788134545649 92-93 0.003822148489046994 94-95 0.0047086862129834955 96-97 0.0033956067539454692 98-99 0.00562867819065345 100 0.006088674179488427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5978313.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.77202044514237 #Duplication Level Percentage of deduplicated Percentage of total 1 92.41631192108042 82.03982731322806 2 5.4848017447932405 9.737938652526763 3 1.1521140586333523 3.0682647830440772 4 0.4111300967370919 1.4598739741263393 5 0.20549521749053792 0.9121112824224505 6 0.10872822736972025 0.5791214653817336 7 0.06532052863309352 0.405904471231314 8 0.04126800227338119 0.29307551532342213 9 0.02738485281284711 0.2187907842410351 >10 0.08559540275635229 1.1420346723226162 >50 0.0014600772808080077 0.08385597864892336 >100 3.700580737006478E-4 0.046186305070319386 >500 1.9812065521487662E-5 0.013014802432951623 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.672712686672645E-5 0.0 0.0 0.0 3 0.0 1.672712686672645E-5 0.0 0.0 0.0 4 0.0 1.672712686672645E-5 0.0 0.0 0.0 5 0.0 5.018138060017935E-5 0.0 0.0 0.0 6 0.0 5.018138060017935E-5 0.0 0.0 0.0 7 0.0 5.018138060017935E-5 0.0 0.0 0.0 8 0.0 6.69085074669058E-5 0.0 0.0 0.0 9 0.0 6.69085074669058E-5 0.0 0.0 0.0 10-11 1.672712686672645E-5 8.363563433363225E-5 0.0 0.0 0.0 12-13 1.672712686672645E-5 1.1708988806708514E-4 0.0 0.0 0.0 14-15 1.672712686672645E-5 2.090890858340806E-4 0.0 0.0 0.0 16-17 1.672712686672645E-5 2.9272472016771286E-4 0.0 1.672712686672645E-5 0.0 18-19 1.672712686672645E-5 3.2617897390116576E-4 0.0 1.672712686672645E-5 0.0 20-21 1.672712686672645E-5 3.429061007678922E-4 0.0 5.8544944033542575E-5 0.0 22-23 1.672712686672645E-5 3.763603545013451E-4 0.0 2.3417977613417027E-4 0.0 24-25 1.672712686672645E-5 4.09814608234798E-4 0.0 5.519951866019728E-4 0.0 26-27 1.672712686672645E-5 4.599959888349774E-4 0.0 0.0010705361194704927 0.0 28-29 1.672712686672645E-5 4.683595522683406E-4 0.0 0.0028519751307768595 0.0 30-31 1.672712686672645E-5 4.683595522683406E-4 0.0 0.008581016082630668 0.0 32-33 1.672712686672645E-5 4.683595522683406E-4 0.0 0.021092906978942054 0.0 34-35 1.672712686672645E-5 4.683595522683406E-4 0.0 0.0412323677264807 0.0 36-37 1.672712686672645E-5 5.519951866019728E-4 0.0 0.07194337265379047 0.0 38-39 1.672712686672645E-5 6.356308209356051E-4 0.0 0.11422954937287491 0.0 40-41 1.672712686672645E-5 6.523579478023315E-4 0.0 0.16435238502902072 0.0 42-43 1.672712686672645E-5 6.774486381024211E-4 0.0 0.2173941043234103 0.0 44-45 1.672712686672645E-5 7.025393284025109E-4 0.0 0.2835749817716135 0.0 46-47 1.672712686672645E-5 7.359935821359638E-4 0.0 0.36545426778423945 0.0 48-49 1.672712686672645E-5 7.359935821359638E-4 0.0 0.451030248834412 0.0 50-51 1.672712686672645E-5 7.861749627361431E-4 0.0 0.5428621753327402 0.0 52-53 1.672712686672645E-5 8.029020896028696E-4 0.0 0.6356977294430719 0.0 54-55 1.672712686672645E-5 8.029020896028696E-4 0.0 0.7411455372109155 0.0 56-57 1.672712686672645E-5 8.19629216469596E-4 0.0 0.8555590849793244 0.0 58-59 1.672712686672645E-5 8.19629216469596E-4 0.0 0.9838561480471162 0.0 60-61 1.672712686672645E-5 8.279927799029593E-4 0.0 1.1191116958914664 0.0 62-63 1.672712686672645E-5 8.363563433363225E-4 0.0 1.2587497509748986 0.0 64-65 1.672712686672645E-5 8.363563433363225E-4 0.0 1.4123549569920477 0.0 66-67 1.672712686672645E-5 8.865377239365018E-4 0.0 1.588533420715844 0.0 68-69 1.672712686672645E-5 8.865377239365018E-4 0.0 1.765489695838943 0.0 70-71 1.672712686672645E-5 8.949012873698651E-4 0.0 1.9792038322516736 0.0 72-73 1.672712686672645E-5 0.001078899682903856 0.0 2.2209944511102044 0.0 74-75 1.672712686672645E-5 0.0011207175000706722 0.0 2.446517939090844 0.0 76-77 1.672712686672645E-5 0.0011290810635040355 0.0 2.679744268993611 0.0 78-79 1.672712686672645E-5 0.0011374446269373985 0.0 2.9556749537871303 0.0 80-81 1.672712686672645E-5 0.0011374446269373985 0.0 3.291731296103098 0.0 82-83 1.672712686672645E-5 0.0011458081903707618 0.0 3.6420893318901166 0.0 84-85 1.672712686672645E-5 0.001154171753804125 0.0 3.996804784225918 0.0 86-87 1.672712686672645E-5 0.0011792624441042147 0.0 4.422627252872173 0.0 88 1.672712686672645E-5 0.0012043531344043043 0.0 4.779709593659616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6065 0.0 44.64091 1 GTATCAA 9340 0.0 32.38536 1 ATCAACG 9660 0.0 30.088552 3 TCAACGC 9705 0.0 29.896313 4 CAACGCA 10455 0.0 27.849295 5 TATCAAC 10825 0.0 27.134016 2 AACGCAG 11840 0.0 24.634338 6 GTACATG 15755 0.0 24.526562 1 TACATGG 15965 0.0 23.238022 2 TACCTGG 4100 0.0 21.591974 2 ACATGGG 16300 0.0 21.56747 3 GTGGTAT 3705 0.0 20.859161 1 ACGCAGA 14165 0.0 20.409733 7 CGCAGAG 13895 0.0 20.349403 8 GTACCTG 4765 0.0 19.441233 1 TGGTATC 4130 0.0 17.841959 2 CATGGGG 10970 0.0 17.632555 4 GCAGAGT 15795 0.0 16.927704 9 GTACGAC 310 9.506439E-7 16.50701 3 CAGAGTA 15210 0.0 15.7643795 10-11 >>END_MODULE