FastQCFastQC Report
Wed 25 May 2016
SRR2049530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978313
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230050.384807553569042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224790.37600908483714385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138150.23108525766382587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.11128557504433106No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.10737142735751706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121600.058.5031781
GTATCAA225450.041.5785751
GTGGTAT48950.035.1065981
ATCAACG279250.032.9937483
TCAACGC282750.032.635264
TGGTATC51100.032.3220062
TATCAAC288000.032.1710622
CAACGCA292100.031.6973825
AACGCAG308450.030.1078686
CGCAGAG345750.026.6010768
ACGCAGA349650.026.4791037
GCAGAGT372650.024.1511739
GTACATG161200.022.2263531
GAGTACT279100.021.84832412-13
CAGAGTA360300.021.48976910-11
AGAGTAC344950.021.0086810-11
TACATGG165800.020.4626962
TACCTGG45400.020.4184362
AGTACTT290750.019.85756512-13
ACATGGG164950.019.769383
Produced by FastQC (version 0.11.5)