##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049530_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5978313
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831317965452797	33.0	31.0	34.0	30.0	34.0
2	31.909838109848046	33.0	31.0	34.0	30.0	34.0
3	31.930211248557914	33.0	31.0	34.0	30.0	34.0
4	35.59330299367062	37.0	35.0	37.0	33.0	37.0
5	35.721585838680575	37.0	35.0	37.0	33.0	37.0
6	35.790244839974086	37.0	35.0	37.0	35.0	37.0
7	35.78144988393883	37.0	35.0	37.0	33.0	37.0
8	35.75782800264891	37.0	35.0	37.0	33.0	37.0
9	37.467344048396264	39.0	37.0	39.0	34.0	39.0
10-11	37.44281463683818	39.0	37.0	39.0	34.5	39.0
12-13	37.43303462030175	39.0	37.0	39.0	34.0	39.0
14-15	38.690370678149506	40.0	38.0	41.0	34.0	41.0
16-17	38.69412307451952	40.0	38.0	41.0	34.0	41.0
18-19	38.779140954981784	40.0	38.0	41.0	34.5	41.0
20-21	38.810204651379074	40.0	38.0	41.0	35.0	41.0
22-23	38.77693021425944	40.0	38.0	41.0	35.0	41.0
24-25	38.73823309351651	40.0	38.0	41.0	34.0	41.0
26-27	38.63800389842419	40.0	38.0	41.0	34.0	41.0
28-29	38.546488700073084	40.0	38.0	41.0	34.0	41.0
30-31	38.41615904018408	40.0	38.0	41.0	34.0	41.0
32-33	38.246497715994465	40.0	38.0	41.0	34.0	41.0
34-35	38.13188377724619	40.0	38.0	41.0	34.0	41.0
36-37	37.98540884359852	40.0	37.0	41.0	33.0	41.0
38-39	37.890062798652394	40.0	37.0	41.0	33.0	41.0
40-41	37.76366418084834	40.0	37.0	41.0	33.0	41.0
42-43	37.615196460941405	40.0	37.0	41.0	33.0	41.0
44-45	37.41492959635937	40.0	37.0	41.0	32.5	41.0
46-47	37.14435652666563	40.0	37.0	41.0	31.5	41.0
48-49	36.892497599239114	40.0	37.0	41.0	31.0	41.0
50-51	36.6791995501072	40.0	36.0	41.0	31.0	41.0
52-53	36.57588311284471	40.0	36.0	41.0	31.0	41.0
54-55	36.452593649747016	39.5	36.0	41.0	30.0	41.0
56-57	36.2872562042168	39.0	35.0	41.0	30.0	41.0
58-59	36.1178998991856	39.0	35.0	41.0	30.0	41.0
60-61	35.93140623450127	39.0	35.0	41.0	29.5	41.0
62-63	35.727923496143475	39.0	35.0	41.0	29.0	41.0
64-65	35.44885906776711	38.0	35.0	40.0	29.0	41.0
66-67	35.20007584079321	37.5	35.0	40.0	29.0	41.0
68-69	34.89457753382936	37.0	34.5	40.0	28.0	41.0
70-71	34.59998288814921	36.5	34.0	39.5	28.0	41.0
72-73	34.277188230191356	36.0	34.0	39.0	28.0	41.0
74-75	33.94581230524397	36.0	34.0	39.0	28.0	40.5
76-77	33.655349176264274	35.0	34.0	38.5	27.5	40.0
78-79	33.32749029701188	35.0	34.0	37.0	27.0	39.0
80-81	33.018791337957715	35.0	34.0	37.0	26.5	39.0
82-83	32.72567001761199	35.0	33.0	36.0	26.0	39.0
84-85	32.44947780418991	35.0	33.0	36.0	26.0	37.0
86-87	32.16727235593051	35.0	33.0	36.0	26.0	37.0
88-89	31.958658905948887	35.0	33.0	35.0	25.5	37.0
90-91	31.807605004957082	35.0	33.0	35.0	25.0	36.0
92-93	31.64613838385511	35.0	33.0	35.0	25.0	36.0
94-95	31.459667384427682	35.0	33.0	35.0	25.0	36.0
96-97	31.287433929939766	35.0	33.0	35.0	24.5	36.0
98-99	31.107752722214443	35.0	33.0	35.0	24.0	35.0
100	30.99368567687908	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3755098211547008
1101	2	0.4195184718686136
1101	3	0.4984655939851912
1101	4	0.39301144267827226
1101	5	0.3206145544859922
1101	6	0.2992994773262154
1101	7	0.3363395080441265
1101	8	0.3337283981638919
1101	9	0.35777266887485837
1101	10-11	0.3593516580411915
1101	12-13	0.331066841372909
1101	14-15	0.4926690642606033
1101	16-17	0.5097938984155519
1101	18-19	0.47966499944848096
1101	20-21	0.4328922021732353
1101	22-23	0.4876790634479278
1101	24-25	0.5222834189578478
1101	26-27	0.49219288553121743
1101	28-29	0.46176923871585984
1101	30-31	0.48793389656424324
1101	32-33	0.5484120273702331
1101	34-35	0.5630230040111854
1101	36-37	0.5876379638274685
1101	38-39	0.6285052134650186
1101	40-41	0.6130249961823324
1101	42-43	0.49916235316167956
1101	44-45	0.5192945503948678
1101	46-47	0.6479851057796893
1101	48-49	0.6678408863742717
1101	50-51	0.6520139889105891
1101	52-53	0.6259031333853926
1101	54-55	0.571345582432194
1101	56-57	0.5042157408331747
1101	58-59	0.5035454771505101
1101	60-61	0.6170887917695751
1101	62-63	0.5837638997535066
1101	64-65	0.5940773813327027
1101	66-67	0.6004301939408023
1101	68-69	0.527115634934141
1101	70-71	0.5537676681669694
1101	72-73	0.5131312108902719
1101	74-75	0.53027015855141
1101	76-77	0.5189093888817311
1101	78-79	0.4976058723103165
1101	80-81	0.4798215047146428
1101	82-83	0.49083464360912643
1101	84-85	0.5046580659857511
1101	86-87	0.5426973402163355
1101	88-89	0.5389347785366496
1101	90-91	0.5097656546346201
1101	92-93	0.5622997357526032
1101	94-95	0.49129975381950786
1101	96-97	0.5392347718291184
1101	98-99	0.5898687750661651
1101	100	0.643191198773664
1102	1	0.26879866640783234
1102	2	0.3377096658922696
1102	3	0.39857076449110096
1102	4	0.3643364519429042
1102	5	0.3025506646997087
1102	6	0.2465511375072822
1102	7	0.28710020709677764
1102	8	0.29327450373456543
1102	9	0.3611512958582921
1102	10-11	0.3122401546383742
1102	12-13	0.3077920274942656
1102	14-15	0.3953470254377791
1102	16-17	0.43241831900356686
1102	18-19	0.397878183197534
1102	20-21	0.3659246031623766
1102	22-23	0.40542676200787753
1102	24-25	0.3422471072537334
1102	26-27	0.32312096484376696
1102	28-29	0.34940433404109683
1102	30-31	0.4385567674166424
1102	32-33	0.3990360099494197
1102	34-35	0.39169930376164075
1102	36-37	0.4250427517006372
1102	38-39	0.4231937068998306
1102	40-41	0.4230488389777065
1102	42-43	0.4106141499699376
1102	44-45	0.4476476184627032
1102	46-47	0.48263708100569147
1102	48-49	0.5062893797699175
1102	50-51	0.5551407089729778
1102	52-53	0.528104736915914
1102	54-55	0.4441236013241152
1102	56-57	0.4417546890722406
1102	58-59	0.4665970214412596
1102	60-61	0.4601587204499964
1102	62-63	0.48981774459867466
1102	64-65	0.49574085581702576
1102	66-67	0.48378726523311144
1102	68-69	0.462467044714586
1102	70-71	0.5217940962167447
1102	72-73	0.48955414269180153
1102	74-75	0.4793428113656688
1102	76-77	0.4215850315613423
1102	78-79	0.4227055770662105
1102	80-81	0.3830998991599657
1102	82-83	0.4395098612980419
1102	84-85	0.4736533092063695
1102	86-87	0.4534525725109333
1102	88-89	0.4114070653623898
1102	90-91	0.402189484285131
1102	92-93	0.3708317693678822
1102	94-95	0.3958485337267241
1102	96-97	0.3739163907026217
1102	98-99	0.4274343241046772
1102	100	0.48468319544965865
1103	1	0.3000323576192585
1103	2	0.33741411666022714
1103	3	0.43836087283191105
1103	4	0.32227130668762527
1103	5	0.23510856491247978
1103	6	0.2721010934167367
1103	7	0.2634547674364356
1103	8	0.280788757897227
1103	9	0.3741559207367686
1103	10-11	0.3224116474138441
1103	12-13	0.29549756722437337
1103	14-15	0.42359972653593303
1103	16-17	0.4275797427569543
1103	18-19	0.40663030578975423
1103	20-21	0.422230151031755
1103	22-23	0.40286265976452285
1103	24-25	0.3994744743454035
1103	26-27	0.42582275933798286
1103	28-29	0.44071380644402325
1103	30-31	0.4462380035596425
1103	32-33	0.4259882193717317
1103	34-35	0.43112921147589134
1103	36-37	0.4890886205411604
1103	38-39	0.4678229543984571
1103	40-41	0.5014725650095784
1103	42-43	0.48564149911515386
1103	44-45	0.6010869975958215
1103	46-47	0.6240112715185262
1103	48-49	0.5750761076412871
1103	50-51	0.5660087313966926
1103	52-53	0.5222399735396053
1103	54-55	0.4888266944336337
1103	56-57	0.509383466894441
1103	58-59	0.49496780880142666
1103	60-61	0.5076278341375513
1103	62-63	0.472001208785926
1103	64-65	0.51417548898322
1103	66-67	0.551226571312867
1103	68-69	0.5497795348219441
1103	70-71	0.574615515596939
1103	72-73	0.6126699329432483
1103	74-75	0.5850774255012965
1103	76-77	0.5960394496804398
1103	78-79	0.5385884586778928
1103	80-81	0.543269152799752
1103	82-83	0.5105529249226848
1103	84-85	0.5477734956692544
1103	86-87	0.5736644497872874
1103	88-89	0.5476158784003147
1103	90-91	0.5017710411123915
1103	92-93	0.5148758060366632
1103	94-95	0.5485702844656366
1103	96-97	0.5402318216249107
1103	98-99	0.5418318706791183
1103	100	0.5610008204747388
1104	1	0.22192682278418374
1104	2	0.2661396664801359
1104	3	0.35766667296252663
1104	4	0.27322404260608835
1104	5	0.23182679305011789
1104	6	0.2360077459924952
1104	7	0.211857973860468
1104	8	0.23911014933037222
1104	9	0.26807477682240943
1104	10-11	0.278935822586007
1104	12-13	0.2539850331542084
1104	14-15	0.3478816449844544
1104	16-17	0.3759991011967685
1104	18-19	0.39164033486761696
1104	20-21	0.3385094076280808
1104	22-23	0.39903690919358326
1104	24-25	0.38766925727607315
1104	26-27	0.43029906544293084
1104	28-29	0.3831972990838963
1104	30-31	0.4077780416000252
1104	32-33	0.3965309938283781
1104	34-35	0.3457580498038624
1104	36-37	0.39000783970293185
1104	38-39	0.45131445568436845
1104	40-41	0.41936408404681913
1104	42-43	0.4111476609238238
1104	44-45	0.3807201477176321
1104	46-47	0.4226315663304234
1104	48-49	0.38673698394268996
1104	50-51	0.4422924194706539
1104	52-53	0.40003321312133977
1104	54-55	0.40856881082007845
1104	56-57	0.4017727006067986
1104	58-59	0.40816994751895663
1104	60-61	0.3710780307472987
1104	62-63	0.35110739542104596
1104	64-65	0.37046036012615247
1104	66-67	0.38961487657558536
1104	68-69	0.362264561108951
1104	70-71	0.3987705586375583
1104	72-73	0.3660908239710494
1104	74-75	0.35400012100053146
1104	76-77	0.34379243949799587
1104	78-79	0.3145378106166561
1104	80-81	0.28616035938816964
1104	82-83	0.3051983675228698
1104	84-85	0.31822962647664355
1104	86-87	0.32708613517651486
1104	88-89	0.31739974112787905
1104	90-91	0.3106535059121214
1104	92-93	0.3454896959652842
1104	94-95	0.30906681755460497
1104	96-97	0.32320293144103474
1104	98-99	0.33962126343323007
1104	100	0.4112294800607863
1105	1	0.24654757128890026
1105	2	0.23336892685107102
1105	3	0.3425560374257337
1105	4	0.2795743343676236
1105	5	0.23967910554095084
1105	6	0.24477204125403063
1105	7	0.22614720685064071
1105	8	0.2469720365764303
1105	9	0.2436067178005814
1105	10-11	0.23647325227994997
1105	12-13	0.2586130848161403
1105	14-15	0.323055647163244
1105	16-17	0.26486907136001037
1105	18-19	0.2521771858207984
1105	20-21	0.2367526832116269
1105	22-23	0.2800756921195955
1105	24-25	0.30228148880804184
1105	26-27	0.30067088713079926
1105	28-29	0.31187981073773585
1105	30-31	0.2851525555672083
1105	32-33	0.2598397110908195
1105	34-35	0.2506529939683446
1105	36-37	0.3524542058756168
1105	38-39	0.34484535590184606
1105	40-41	0.33458644025932927
1105	42-43	0.3448072468648036
1105	44-45	0.3773794193634714
1105	46-47	0.3636820274182071
1105	48-49	0.4021721479557172
1105	50-51	0.4220628107610125
1105	52-53	0.41038532195864974
1105	54-55	0.36807681632865297
1105	56-57	0.41334665459771713
1105	58-59	0.416031556134584
1105	60-61	0.4033543786499294
1105	62-63	0.396097042526975
1105	64-65	0.39736440912295734
1105	66-67	0.42212066398319337
1105	68-69	0.40711311989044674
1105	70-71	0.4183271228465699
1105	72-73	0.41696917351426066
1105	74-75	0.4587699550300002
1105	76-77	0.3530460351478766
1105	78-79	0.37401353045586916
1105	80-81	0.3656913919297722
1105	82-83	0.39142706799859184
1105	84-85	0.41594327118168906
1105	86-87	0.4543436496875941
1105	88-89	0.41150053685590393
1105	90-91	0.354703991114107
1105	92-93	0.3634934527142164
1105	94-95	0.38272356045185774
1105	96-97	0.4241372147792184
1105	98-99	0.47823735060261185
1105	100	0.44583061906151045
1106	1	0.1414399296879516
1106	2	0.17486129564343145
1106	3	0.2758243076013507
1106	4	0.23305286431294547
1106	5	0.17356132888998843
1106	6	0.1884353909856955
1106	7	0.16636482067310965
1106	8	0.2138536032573768
1106	9	0.24192528528779178
1106	10-11	0.2263217658794332
1106	12-13	0.19623955641407065
1106	14-15	0.29647682888235494
1106	16-17	0.2667016792614447
1106	18-19	0.23429075554415135
1106	20-21	0.23938555614241608
1106	22-23	0.23025316572690002
1106	24-25	0.2785881913893675
1106	26-27	0.3037940958599137
1106	28-29	0.24898228639220576
1106	30-31	0.3117721203395618
1106	32-33	0.2993572135789151
1106	34-35	0.34399454483886416
1106	36-37	0.3620487251378819
1106	38-39	0.38061920066991206
1106	40-41	0.3922277656265578
1106	42-43	0.37248484251473
1106	44-45	0.3755156118538352
1106	46-47	0.33305188588025203
1106	48-49	0.3351888528361613
1106	50-51	0.3687369718950819
1106	52-53	0.3814573798651182
1106	54-55	0.27670517131996064
1106	56-57	0.3429707711665415
1106	58-59	0.33826884559135806
1106	60-61	0.36533790210731354
1106	62-63	0.38742729146244415
1106	64-65	0.3696614207319655
1106	66-67	0.35862503232202414
1106	68-69	0.38648998202479135
1106	70-71	0.3650368141338376
1106	72-73	0.39217158712381917
1106	74-75	0.3675970229833396
1106	76-77	0.3761813812135344
1106	78-79	0.3086274190648055
1106	80-81	0.22761275238229217
1106	82-83	0.28494398625679906
1106	84-85	0.31749088985436913
1106	86-87	0.3677230062158543
1106	88-89	0.3086763719424468
1106	90-91	0.3013391773432872
1106	92-93	0.33125973005176945
1106	94-95	0.3283821103649309
1106	96-97	0.37921903058460416
1106	98-99	0.4399007089953635
1106	100	0.4616776579135866
1107	1	0.1785120552979791
1107	2	0.22032639340072535
1107	3	0.2708609532181647
1107	4	0.22195332200595175
1107	5	0.207117007041127
1107	6	0.15534964841680932
1107	7	0.18130224981511134
1107	8	0.180822317255398
1107	9	0.1580758759111589
1107	10-11	0.1663588187864775
1107	12-13	0.17200560823322064
1107	14-15	0.24373752987231256
1107	16-17	0.23286149479382345
1107	18-19	0.21942407696595012
1107	20-21	0.2246825098788321
1107	22-23	0.2520299672389399
1107	24-25	0.2882992106100275
1107	26-27	0.2537677413644204
1107	28-29	0.28091165653528094
1107	30-31	0.2773264557221111
1107	32-33	0.3366565121229428
1107	34-35	0.2914577569330774
1107	36-37	0.33409672560543413
1107	38-39	0.29301355349766567
1107	40-41	0.3182931041482533
1107	42-43	0.2920001733394457
1107	44-45	0.34253960607136236
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2216	4	-1.139876435488091
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2216	40-41	-0.8841547714334155
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2216	60-61	-1.3945633784178213
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2216	64-65	-1.2348283321258364
2216	66-67	-1.1324317768226777
2216	68-69	-1.4507789753201195
2216	70-71	-1.3152909841911011
2216	72-73	-1.1089111707530321
2216	74-75	-1.1347080956843882
2216	76-77	-1.2857865163515996
2216	78-79	-1.3973813594364053
2216	80-81	-1.1194732130651133
2216	82-83	-1.196580261449661
2216	84-85	-1.2982670798818923
2216	86-87	-1.3778709646814846
2216	88-89	-1.1761081452104847
2216	90-91	-1.0829056186069366
2216	92-93	-1.137742036814025
2216	94-95	-1.233504488309574
2216	96-97	-1.3157352375881537
2216	98-99	-1.3575045485310575
2216	100	-1.3248834211381606
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	367.0
3	52.0
4	80.0
5	137.0
6	469.0
7	1155.0
8	2498.0
9	4078.0
10	5742.0
11	8272.0
12	12131.0
13	17460.0
14	19366.0
15	23875.0
16	30622.0
17	43554.0
18	43455.0
19	23630.0
20	17371.0
21	16701.0
22	17323.0
23	18647.0
24	21822.0
25	26556.0
26	33782.0
27	43498.0
28	56809.0
29	73806.0
30	96306.0
31	123954.0
32	159467.0
33	207012.0
34	280590.0
35	406305.0
36	647928.0
37	1185966.0
38	1651731.0
39	653908.0
40	1888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.232802529008346	20.5390544872224	14.652462194752925	22.575680789016328
2	15.909573715363514	20.979387806512413	39.72615319456715	23.384885283556915
3	17.920891729824113	25.7598924646468	31.259888868314523	25.05932693721456
4	12.664542489734988	18.022495777268897	38.17407532823089	31.13888640476523
5	12.241864837581119	37.31282665477906	35.3834045781224	15.061903929517422
6	29.2806986965012	38.53041157080588	17.837004271172447	14.351885461520473
7	25.53474533702066	33.34693248747598	22.87203764674081	18.246284528762548
8	24.5662948728178	35.61561597728322	20.969126240128276	18.8489629097707
9	24.29748540975627	16.892265539926836	22.6082789283259	36.201970121990996
10-11	23.22937925799469	27.111143227194695	29.02491890270717	20.634558612103447
12-13	24.33836245722245	24.756716403679903	29.38483795841334	21.52008318068431
14-15	21.598344014301695	26.854894253185996	27.526538634439067	24.02022309807324
16-17	20.102886884644548	29.984253082767665	28.238969756183725	21.673890276404062
18-19	19.907438770770284	27.915308214876	31.61464613846749	20.562606875886225
20-21	21.121978725436424	27.281057381906905	31.42800485688856	20.168959035768115
22-23	20.770449790768733	27.271890916383935	31.548113991355088	20.409545301492244
24-25	20.58209397868596	27.167680916004233	31.609226549362674	20.64099855594714
26-27	20.367242397646294	27.147282184790257	31.65801121486948	20.82746420269397
28-29	20.492460573279452	27.146636522118474	31.735152064815686	20.625750839786388
30-31	20.85856172669808	27.639895318075762	31.19880808935583	20.302734865870324
32-33	20.336835882770924	27.359771453881965	31.692282035464192	20.61111062788292
34-35	20.522353045081445	27.002249631292308	31.58755655650683	20.88784076711942
36-37	21.045594300599518	27.363129029878497	30.964780532568305	20.626496136953687
38-39	20.847520027807175	27.45537077098506	31.14447169293411	20.552637508273655
40-41	20.638196762196962	27.283574814500344	31.18495970351502	20.89326871978767
42-43	20.643164718876378	27.36657481801304	30.97561971077794	21.01464075233264
44-45	21.01137896259363	27.605940003475897	30.630196177416607	20.75248485651387
46-47	21.003182670428934	27.97562623435742	30.068164714694596	20.953026380519052
48-49	21.037757641662456	27.69130689544024	30.049840147212098	21.22109531568521
50-51	21.114476609036696	27.649773439430152	29.801601220946445	21.434148730586706
52-53	21.062973785414048	27.914455131405802	29.631904518883506	21.39066656429665
54-55	21.207379071654493	28.332951118484427	29.44941156476752	21.010258245093556
56-57	20.942831196693785	28.007357594023595	29.489640304882	21.56017090440062
58-59	20.769326886019847	27.61123388840715	29.904785705109678	21.71465352046333
60-61	21.173456458368776	27.919991810398688	29.51207974557371	21.394471985658832
62-63	20.945549354809625	27.903733043084227	29.653791964388613	21.496925637717528
64-65	20.886502596970082	27.668231823927584	29.873076234047968	21.572189345054365
66-67	20.81968608870094	27.724485151580385	29.65623412491116	21.799594634807512
68-69	21.24595182620917	27.980000378033065	29.339045981700856	21.43500181405691
70-71	21.239026795686343	27.621772229055253	29.432826618479158	21.706374356779246
72-73	20.945524264119324	27.77700832994191	29.697332675622707	21.58013473031606
74-75	21.005373924048474	27.820523950485697	29.443582161054465	21.730519964411364
76-77	21.075845309537993	27.87242822515315	29.191278542960198	21.860447922348662
78-79	21.13862235331478	27.930298286127492	29.3341413663592	21.59693799419852
80-81	20.99883612359237	27.701780674031305	29.421375448329947	21.878007754046386
82-83	20.76285160018517	27.761003664507882	29.56033850802717	21.91580622727978
84-85	20.88664477754845	27.711897988613178	29.295647451045138	22.10580978279324
86-87	21.024049761195172	27.729996739882974	29.24909585697504	21.996857641946818
88-89	20.808819015544362	27.59056114362774	29.338572161459037	22.262047679368866
90-91	20.618372185178117	27.505085468148266	29.309040399216567	22.56750194745705
92-93	20.726706282330607	27.70183706838511	29.145280202064978	22.426176447219305
94-95	20.634852398389736	27.463172256395513	29.332441659767376	22.569533685447375
96-97	20.52074724090224	27.459335100052474	29.336160552316347	22.683757106728937
98-99	20.495310423352812	27.385006619343937	29.277112776743063	22.84257018056018
100	20.624005665874964	27.49652197374916	29.049017211901752	22.830455148474126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1961.0
1	1518.0
2	3088.5
3	4860.5
4	2976.5
5	1367.5
6	2508.5
7	6059.5
8	9734.0
9	10963.5
10	8681.0
11	5633.5
12	5049.5
13	5431.5
14	5972.0
15	6647.5
16	7306.5
17	8070.5
18	9047.0
19	10138.5
20	11561.5
21	13707.5
22	17154.0
23	22851.0
24	29516.0
25	37301.5
26	48629.5
27	63340.0
28	82624.5
29	101110.0
30	115047.5
31	129569.0
32	144339.0
33	158150.0
34	170800.0
35	182354.5
36	190831.5
37	197361.0
38	203948.5
39	207683.0
40	210507.0
41	209808.0
42	204315.5
43	201354.5
44	198482.0
45	195348.0
46	192927.0
47	188646.5
48	183910.0
49	181420.5
50	178137.0
51	169589.5
52	158635.5
53	150261.5
54	144925.5
55	137392.0
56	127291.0
57	115990.5
58	104910.5
59	95050.5
60	82590.0
61	68075.0
62	55584.5
63	44446.5
64	34478.0
65	26483.5
66	20248.0
67	15181.5
68	11152.0
69	8202.5
70	5888.5
71	4216.5
72	3098.5
73	2156.0
74	1449.0
75	898.0
76	521.0
77	340.5
78	213.0
79	126.0
80	63.0
81	31.0
82	19.5
83	9.5
84	5.0
85	2.5
86	3.0
87	2.5
88	1.5
89	1.5
90	1.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058227128623074775
2	1.1708988806708514E-4
3	0.0
4	0.118076788552222
5	0.001639258432939192
6	0.0015890770523390126
7	0.0
8	0.0
9	5.018138060017935E-5
10-11	0.0
12-13	3.3454253733452904E-5
14-15	8.363563433363226E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.1708988806708514E-4
30-31	2.090890858340806E-4
32-33	1.6727126866726452E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.5054414180053804E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.5890770523390127E-4
80-81	2.509069030008967E-4
82-83	3.429061007678922E-4
84-85	0.0
86-87	0.0
88-89	6.690850746690581E-5
90-91	6.690850746690581E-5
92-93	6.356308209356051E-4
94-95	6.941757649691476E-4
96-97	0.0
98-99	2.5090690300089675E-5
100	0.0011039903732039456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5978313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.34125919443173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93984520033214	46.42647141612696
2	14.078636548542011	16.990453141595463
3	4.068432854330936	7.364830841349741
4	1.7081996082594446	4.122996612712396
5	0.8703577360477487	2.6259240871368004
6	0.5233793907175119	1.8948822883385483
7	0.36516111996809425	1.5423997251406627
8	0.2531170804192101	1.2218722684890702
9	0.17954622195610942	0.9750640604791109
>10	0.9406311887310824	9.962504030021524
>50	0.04858923905608358	1.9893111763257938
>100	0.02188800588423259	2.4273999494673575
>500	0.0013848918042938612	0.5486258927945342
>1k	6.647311610872478E-4	0.6122399302142886
>5k	8.309139503402075E-5	0.3039723703013526
>10k+	8.309139503402075E-5	0.9910522095062627
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23005	0.384807553569042	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22479	0.37600908483714385	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13815	0.23108525766382587	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6653	0.11128557504433106	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6419	0.10737142735751706	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.34542537334529E-5	0.0	0.0	0.0
3	0.0	3.34542537334529E-5	0.0	0.0	0.0
4	0.0	3.34542537334529E-5	0.0	0.0	0.0
5	0.0	6.69085074669058E-5	0.0	0.0	0.0
6	0.0	6.69085074669058E-5	0.0	0.0	0.0
7	0.0	6.69085074669058E-5	0.0	0.0	0.0
8	0.0	6.69085074669058E-5	0.0	0.0	0.0
9	0.0	6.69085074669058E-5	0.0	0.0	0.0
10-11	0.0	6.69085074669058E-5	0.0	0.0	0.0
12-13	0.0	1.2545345150044836E-4	0.0	0.0	0.0
14-15	0.0	1.7563483210062772E-4	0.0	0.0	0.0
16-17	0.0	2.2581621270080708E-4	0.0	0.0	0.0
18-19	0.0	2.676340298676232E-4	0.0	1.672712686672645E-5	0.0
20-21	0.0	2.676340298676232E-4	0.0	4.181781716681612E-5	0.0
22-23	0.0	2.676340298676232E-4	0.0	2.090890858340806E-4	0.0
24-25	0.0	2.759975933009864E-4	0.0	5.519951866019728E-4	0.0
26-27	0.0	3.1781541046780253E-4	0.0	0.0010872632463372193	0.0
28-29	0.0	3.1781541046780253E-4	0.0	0.002868702257643586	0.0
30-31	0.0	3.2617897390116576E-4	0.0	0.008756650914731295	0.0
32-33	0.0	3.34542537334529E-4	0.0	0.021377268135676402	0.0
34-35	0.0	3.34542537334529E-4	0.0	0.041658909461582225	0.0
36-37	0.0	3.679967910679819E-4	0.0	0.0726626391090597	0.0
38-39	0.0	4.2654173510152447E-4	0.0	0.11533353974607888	0.0
40-41	0.0	4.599959888349774E-4	0.0	0.1656738280514921	0.0
42-43	0.0	4.8508667913506705E-4	0.0	0.21915881620784994	0.0
44-45	0.0	4.8508667913506705E-4	0.0	0.28576623539115464	0.0
46-47	0.0	5.018138060017935E-4	0.0	0.3678546104896147	0.0
48-49	0.0	5.185409328685199E-4	0.0	0.45410804017788964	0.0
50-51	0.0	5.352680597352464E-4	0.0	0.5464585076090864	0.0
52-53	0.0	5.352680597352464E-4	0.0	0.6398627840328869	0.0
54-55	0.0	5.519951866019728E-4	0.0	0.7497516439838463	0.0
56-57	0.0	5.687223134686993E-4	0.0	0.8807919558577813	0.0
58-59	0.0	6.523579478023315E-4	0.0	1.0278568552700402	0.0
60-61	0.0	6.523579478023315E-4	0.0	1.1829675027051945	0.0
62-63	0.0	6.941757649691477E-4	0.0	1.3421177512786633	0.0
64-65	0.0	7.276300187026006E-4	0.0	1.5195507495174643	0.0
66-67	0.0	7.945385261695064E-4	0.0	1.7285561997172112	0.0
68-69	0.0	8.029020896028696E-4	0.0	1.9619999822692455	0.0
70-71	0.0	8.279927799029593E-4	0.0	2.215917768106153	0.0
72-73	0.0	8.865377239365018E-4	0.0	2.4921160869295402	0.0
74-75	0.0	9.28355541103318E-4	0.0	2.779120129708833	0.0
76-77	0.0	9.701733582701341E-4	0.0	3.0892745160716744	0.0
78-79	0.0	9.785369217034973E-4	0.0	3.4426601618215704	0.0
80-81	0.0	9.952640485702238E-4	0.0	3.829491697741487	0.0
82-83	0.0	0.0010119911754369502	0.0	4.232632182356461	0.0
84-85	0.0	0.0010538089926037664	0.0	4.641192256076255	0.0
86-87	0.0	0.001087263246337219	0.0	5.083507671813102	0.0
88	0.0	0.0011039903732039456	0.0	5.444813612134393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12160	0.0	58.503178	1
GTATCAA	22545	0.0	41.578575	1
GTGGTAT	4895	0.0	35.106598	1
ATCAACG	27925	0.0	32.993748	3
TCAACGC	28275	0.0	32.63526	4
TGGTATC	5110	0.0	32.322006	2
TATCAAC	28800	0.0	32.171062	2
CAACGCA	29210	0.0	31.697382	5
AACGCAG	30845	0.0	30.107868	6
CGCAGAG	34575	0.0	26.601076	8
ACGCAGA	34965	0.0	26.479103	7
GCAGAGT	37265	0.0	24.151173	9
GTACATG	16120	0.0	22.226353	1
GAGTACT	27910	0.0	21.848324	12-13
CAGAGTA	36030	0.0	21.489769	10-11
AGAGTAC	34495	0.0	21.00868	10-11
TACATGG	16580	0.0	20.462696	2
TACCTGG	4540	0.0	20.418436	2
AGTACTT	29075	0.0	19.857565	12-13
ACATGGG	16495	0.0	19.76938	3
>>END_MODULE