FastQCFastQC Report
Wed 25 May 2016
SRR2049529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8201420
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75650.041.87911
GTATCAA123200.035.47831
TCAACGC127950.032.7546044
ATCAACG128900.032.5925983
CAACGCA141350.029.7218675
TATCAAC148050.028.8261342
AACGCAG156150.026.805766
GTACATG227600.025.9282191
TACATGG232150.024.7536812
ACATGGG236900.023.3069323
ACGCAGA184650.022.556777
CGCAGAG185300.022.4610548
GTGGTAT48550.021.1895831
CATGGGG155050.019.6039434
GCAGAGT213300.018.4096959
GAGTACA173800.017.4630681
TGGTATC53700.017.3430652
AGAGTAC184650.016.31708710-11
CATGGGT67050.016.179534
TACCTGG49600.015.9911152
Produced by FastQC (version 0.11.5)