##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049529_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8201420 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.060798739730437 31.0 31.0 34.0 28.0 34.0 2 31.149583730622258 31.0 31.0 34.0 28.0 34.0 3 31.30839598508551 31.0 31.0 34.0 28.0 34.0 4 34.89095290815493 37.0 35.0 37.0 32.0 37.0 5 34.90987255865448 37.0 35.0 37.0 32.0 37.0 6 34.92432554362537 37.0 35.0 37.0 32.0 37.0 7 30.905013400118516 37.0 33.0 37.0 2.0 37.0 8 32.709313143333716 37.0 33.0 37.0 17.0 37.0 9 35.53999283050984 39.0 35.0 39.0 30.0 39.0 10-11 36.09687548009978 39.0 36.0 39.0 32.0 39.0 12-13 36.27240081595626 39.0 37.0 39.0 32.0 39.0 14-15 37.48863916248649 40.0 38.0 41.0 32.0 41.0 16-17 37.50963144187226 40.0 38.0 41.0 32.0 41.0 18-19 37.51340322773373 40.0 38.0 41.0 32.0 41.0 20-21 37.5840147803673 40.0 38.0 41.0 32.0 41.0 22-23 37.5414093535022 40.0 38.0 41.0 32.0 41.0 24-25 37.51975255504534 40.0 38.0 41.0 32.0 41.0 26-27 37.54123487152225 40.0 38.0 41.0 32.0 41.0 28-29 37.52135849645549 40.0 38.0 41.0 32.0 41.0 30-31 37.439997829644135 40.0 38.0 41.0 32.0 41.0 32-33 37.38570070792619 40.0 37.0 41.0 31.0 41.0 34-35 37.3215471223276 40.0 37.0 41.0 31.0 41.0 36-37 37.14765016302055 40.0 37.0 41.0 30.5 41.0 38-39 37.03105846304665 40.0 37.0 41.0 30.0 41.0 40-41 36.95703926880955 40.0 37.0 41.0 30.0 41.0 42-43 36.84383807925944 40.0 37.0 41.0 30.0 41.0 44-45 36.734444888811936 40.0 36.5 41.0 30.0 41.0 46-47 36.58093958119447 40.0 36.0 41.0 30.0 41.0 48-49 36.49829877021296 40.0 36.0 41.0 30.0 41.0 50-51 36.36426978498845 40.0 36.0 41.0 30.0 41.0 52-53 36.24497660161289 39.0 35.5 41.0 30.0 41.0 54-55 36.12575803946146 39.0 35.0 41.0 29.0 41.0 56-57 35.92923585184029 39.0 35.0 41.0 29.0 41.0 58-59 35.747467694618734 39.0 35.0 41.0 29.0 41.0 60-61 35.52574450521983 39.0 35.0 40.5 28.0 41.0 62-63 35.2879349551663 38.0 35.0 40.0 28.0 41.0 64-65 34.929684188835594 38.0 34.0 40.0 27.5 41.0 66-67 31.40564792438383 36.5 31.0 40.0 7.0 41.0 68-69 31.766677782627887 36.0 31.0 39.5 11.0 41.0 70-71 31.921749831126807 35.5 30.5 39.0 15.5 41.0 72-73 33.16134992476913 35.0 32.5 39.0 25.5 41.0 74-75 33.22733282772983 35.0 33.0 38.5 26.0 40.0 76-77 32.898859892555194 35.0 33.0 37.5 25.5 39.5 78-79 29.442437529110812 35.0 29.0 37.0 6.5 39.0 80-81 31.212937820523763 35.0 30.0 36.0 21.0 39.0 82-83 31.572865247725396 35.0 32.0 36.0 22.5 37.5 84-85 29.678688385669798 35.0 30.5 35.5 11.0 37.0 86-87 29.2495899490576 35.0 28.5 35.0 10.5 37.0 88-89 30.420047016248404 35.0 30.5 35.0 16.5 36.0 90-91 30.490419585876595 35.0 32.0 35.0 8.5 36.0 92-93 30.265859509694664 35.0 32.0 35.0 2.0 36.0 94-95 29.98627762021699 35.0 31.5 35.0 2.0 36.0 96-97 29.718604705038885 35.0 31.0 35.0 2.0 35.0 98-99 29.457143701944297 35.0 31.0 35.0 2.0 35.0 100 29.215975526189368 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35464494215398545 1101 2 0.3372625117657577 1101 3 0.3562184656271796 1101 4 0.35629422526098153 1101 5 0.37842364088800196 1101 6 0.38516133933821095 1101 7 -12.194834745204432 1101 8 -6.573068416166599 1101 9 -2.1747733951013544 1101 10-11 -0.12319326762187899 1101 12-13 0.3801766119706471 1101 14-15 0.6026473326183179 1101 16-17 0.6303533191274724 1101 18-19 0.6456746518589895 1101 20-21 0.6649892430028146 1101 22-23 0.6008254429205593 1101 24-25 0.6374078288149363 1101 26-27 0.6070008060349608 1101 28-29 0.562289606282306 1101 30-31 0.6211199865297274 1101 32-33 0.6318487747933332 1101 34-35 0.6290707052246205 1101 36-37 0.6725548287463567 1101 38-39 0.6819821890266624 1101 40-41 0.6627619916021317 1101 42-43 0.6450401301750688 1101 44-45 0.5322051324362178 1101 46-47 0.608561901316925 1101 48-49 0.5649459852068404 1101 50-51 0.6615141695842865 1101 52-53 0.6529931415529973 1101 54-55 0.550099578685483 1101 56-57 0.6022965314635584 1101 58-59 0.5647344525457356 1101 60-61 0.5454145002888424 1101 62-63 0.5379842640653081 1101 64-65 0.40109436464955905 1101 66-67 -10.327057347490243 1101 68-69 -8.155381119785602 1101 70-71 -6.698649468073057 1101 72-73 -1.453257155643307 1101 74-75 -0.13199314203903612 1101 76-77 0.11615327532059183 1101 78-79 3.322787007880862 1101 80-81 1.1376079457767112 1101 82-83 0.4498733686944618 1101 84-85 -5.413555933425069 1101 86-87 -5.909667351426197 1101 88-89 -1.3002977899307702 1101 90-91 -0.004990767584580169 1101 92-93 0.34593279589733683 1101 94-95 0.39839438899182156 1101 96-97 0.45106040820560267 1101 98-99 0.48187624487981395 1101 100 0.5671625973973917 1102 1 0.27143001245931586 1102 2 0.2799809092056904 1102 3 0.34546614584011337 1102 4 0.2725350766312076 1102 5 0.33037085086371576 1102 6 0.31541681276443967 1102 7 -12.208913670987506 1102 8 -6.6322804045680215 1102 9 -2.202199212667878 1102 10-11 -0.1251194375848783 1102 12-13 0.39850920845182713 1102 14-15 0.5411555037550002 1102 16-17 0.6040359764354406 1102 18-19 0.6080577475210234 1102 20-21 0.5663409671094328 1102 22-23 0.553160096750382 1102 24-25 0.5859036821742691 1102 26-27 0.5840854107915021 1102 28-29 0.5774414846900697 1102 30-31 0.534982248594936 1102 32-33 0.5215772759332182 1102 34-35 0.5702554726723648 1102 36-37 0.6758912679311777 1102 38-39 0.6496584126262874 1102 40-41 0.5548015237545272 1102 42-43 0.564848575950748 1102 44-45 0.5751188709784714 1102 46-47 0.5811888169761517 1102 48-49 0.48333059914101995 1102 50-51 0.5512500485113065 1102 52-53 0.5607333112249577 1102 54-55 0.5275422551326159 1102 56-57 0.5641264784041766 1102 58-59 0.5071962257636287 1102 60-61 0.5081327930267392 1102 62-63 0.4859148538439868 1102 64-65 0.3898540106139947 1102 66-67 -10.33121859316277 1102 68-69 -6.621231590085365 1102 70-71 -6.223156785934485 1102 72-73 -0.9511124317664255 1102 74-75 0.011341683993208562 1102 76-77 0.1830682641719079 1102 78-79 3.4066089082385247 1102 80-81 1.2560906900707671 1102 82-83 0.6053672743643261 1102 84-85 -5.312819929087258 1102 86-87 -5.79436985380018 1102 88-89 -1.1703301011637741 1102 90-91 0.151432797174067 1102 92-93 0.5124798428065809 1102 94-95 0.5336369175867084 1102 96-97 0.6060681211782288 1102 98-99 0.604209174832345 1102 100 0.6780798089895015 1103 1 0.2392687270947178 1103 2 0.18145458131748882 1103 3 0.22373823808884197 1103 4 0.19296706980571798 1103 5 0.21467049538141936 1103 6 0.24700648309312356 1103 7 -12.372313053105344 1103 8 -6.76633072646122 1103 9 -2.365340788861161 1103 10-11 -0.29087951579747795 1103 12-13 0.2080732511406893 1103 14-15 0.38567414641388353 1103 16-17 0.4174684709811771 1103 18-19 0.40308001368857305 1103 20-21 0.44625889404890273 1103 22-23 0.42530895195771024 1103 24-25 0.5123041945745257 1103 26-27 0.5038812756303486 1103 28-29 0.44688307976856123 1103 30-31 0.425548728068911 1103 32-33 0.49506043134665134 1103 34-35 0.4396873406306838 1103 36-37 0.47042194957707295 1103 38-39 0.5403825444376125 1103 40-41 0.5340489214221762 1103 42-43 0.48407247718612467 1103 44-45 0.4487241576101866 1103 46-47 0.4656792277487085 1103 48-49 0.40817733443497417 1103 50-51 0.4308202271807744 1103 52-53 0.3996795280559624 1103 54-55 0.3870268244194577 1103 56-57 0.4274314821331089 1103 58-59 0.40210039009421905 1103 60-61 0.3228736299231372 1103 62-63 0.2613701091359033 1103 64-65 0.12972167321859018 1103 66-67 -10.61551582303089 1103 68-69 -8.418073565078654 1103 70-71 -6.929613995379523 1103 72-73 -1.6699017973696115 1103 74-75 -0.42875317613393094 1103 76-77 -0.11149885321047748 1103 78-79 3.0966251768097663 1103 80-81 0.8986892438967651 1103 82-83 0.15870813496927028 1103 84-85 -5.657187287469849 1103 86-87 -6.12998725795088 1103 88-89 -1.5274683341473363 1103 90-91 -0.18516518410737604 1103 92-93 0.19298438808278107 1103 94-95 0.27209671733554686 1103 96-97 0.3864944562373829 1103 98-99 0.3970790512579967 1103 100 0.4661820484406931 1104 1 0.179690005911894 1104 2 0.19081814612857428 1104 3 0.21284204183696076 1104 4 0.20017726547889936 1104 5 0.16386151202964072 1104 6 0.21354062525864492 1104 7 -12.310695656744088 1104 8 -6.726635684450223 1104 9 -2.448139990188608 1104 10-11 -0.4038944057403242 1104 12-13 0.1871094045020385 1104 14-15 0.3409740806425603 1104 16-17 0.35796334556287945 1104 18-19 0.3875048142414883 1104 20-21 0.39664787829969583 1104 22-23 0.3650994708614448 1104 24-25 0.48776649666881156 1104 26-27 0.46106867040596455 1104 28-29 0.4028728552313936 1104 30-31 0.37964206563783165 1104 32-33 0.3447593923996308 1104 34-35 0.3424685724796319 1104 36-37 0.4271441002758891 1104 38-39 0.4280129572838334 1104 40-41 0.4388769457339947 1104 42-43 0.33244269848319874 1104 44-45 0.33081812045168135 1104 46-47 0.3434710156214038 1104 48-49 0.29392413213783897 1104 50-51 0.3273825884956878 1104 52-53 0.2998656882366859 1104 54-55 0.27982645478305557 1104 56-57 0.28962197014850943 1104 58-59 0.23586846963939934 1104 60-61 0.1926296758144801 1104 62-63 0.10125937459159928 1104 64-65 0.02000500226962032 1104 66-67 -10.574599549081096 1104 68-69 -8.483931318525364 1104 70-71 -6.909068615366792 1104 72-73 -1.7949107356228353 1104 74-75 -0.5457744150726427 1104 76-77 -0.23535572703356422 1104 78-79 2.904953445251408 1104 80-81 0.7868741340849716 1104 82-83 0.0870995911393031 1104 84-85 -5.633444180728919 1104 86-87 -6.123902230206685 1104 88-89 -1.5840749350889283 1104 90-91 -0.2499224626602512 1104 92-93 0.08482198518916917 1104 94-95 0.154913260651238 1104 96-97 0.23427570528758324 1104 98-99 0.3313862805513246 1104 100 0.37390217163803996 1105 1 0.10107733723584644 1105 2 0.11931779137418275 1105 3 0.11962368666943846 1105 4 0.13829132032953595 1105 5 0.14041633907059747 1105 6 0.19058052615473997 1105 7 -12.30626590852254 1105 8 -6.716613762561263 1105 9 -2.4976492664180796 1105 10-11 -0.43421228216086405 1105 12-13 0.13596426015176633 1105 14-15 0.26818106924771 1105 16-17 0.3413083306725966 1105 18-19 0.31387171318728946 1105 20-21 0.3283472252330313 1105 22-23 0.3055128090562036 1105 24-25 0.35725969470981056 1105 26-27 0.32059942310383605 1105 28-29 0.3173818508323336 1105 30-31 0.3356783025559906 1105 32-33 0.3340688440025872 1105 34-35 0.33255496383448246 1105 36-37 0.40742877453175197 1105 38-39 0.39142238809461816 1105 40-41 0.36976248689505553 1105 42-43 0.30145362216445193 1105 44-45 0.2426666312620398 1105 46-47 0.22349458040608283 1105 48-49 0.1782053776874548 1105 50-51 0.3161060234877979 1105 52-53 0.3145746430409062 1105 54-55 0.2628237843917347 1105 56-57 0.2290327080785204 1105 58-59 0.28226197472318404 1105 60-61 0.19444083322202488 1105 62-63 0.09144557138593257 1105 64-65 -0.029836546834651756 1105 66-67 -10.512754859653228 1105 68-69 -8.508512605883404 1105 70-71 -6.934026541117113 1105 72-73 -1.803783115991461 1105 74-75 -0.56029412385287 1105 76-77 -0.2768316295571225 1105 78-79 2.9064215541373386 1105 80-81 0.8118347214947441 1105 82-83 0.12165148196248765 1105 84-85 -5.605270702158247 1105 86-87 -6.075610113190518 1105 88-89 -1.6222403986696747 1105 90-91 -0.31192166413525513 1105 92-93 0.027420202901261348 1105 94-95 0.08002691860227529 1105 96-97 0.12974719863647266 1105 98-99 0.1555513773934294 1105 100 0.2603858181647212 1106 1 0.035944676305387446 1106 2 0.03309815160431029 1106 3 0.05851998013749693 1106 4 0.009576541330027055 1106 5 0.009231959991879535 1106 6 0.03396324249571592 1106 7 -12.297451289709077 1106 8 -6.807578567929973 1106 9 -2.6256544037065908 1106 10-11 -0.5413115154180375 1106 12-13 0.05767922742449372 1106 14-15 0.2135928329767509 1106 16-17 0.17908613534530105 1106 18-19 0.19762089454288656 1106 20-21 0.2055501027423503 1106 22-23 0.17585108183735798 1106 24-25 0.22466648603931816 1106 26-27 0.22287278510725628 1106 28-29 0.1949937474840553 1106 30-31 0.21257147556875822 1106 32-33 0.1825944146709233 1106 34-35 0.2541030217604856 1106 36-37 0.3101426605499711 1106 38-39 0.2884568886802086 1106 40-41 0.260016420728725 1106 42-43 0.23990918306249398 1106 44-45 0.15519361374491325 1106 46-47 0.22532699867733186 1106 48-49 0.20645624450810374 1106 50-51 0.21811298553017622 1106 52-53 0.22222340495546433 1106 54-55 0.18468302299418582 1106 56-57 0.19732454648389108 1106 58-59 0.13307324213253224 1106 60-61 0.13108529736103236 1106 62-63 0.0697115695392796 1106 64-65 -0.039365600870418405 1106 66-67 -10.53473130042175 1106 68-69 -8.460455191492581 1106 70-71 -6.9735133821432385 1106 72-73 -1.828197254408412 1106 74-75 -0.5840924182674598 1106 76-77 -0.30290892087639065 1106 78-79 2.911615436060572 1106 80-81 0.7290161480572657 1106 82-83 0.06703644960488475 1106 84-85 -5.654138135234053 1106 86-87 -6.113719772878984 1106 88-89 -1.5727186757408091 1106 90-91 -0.28246764227043286 1106 92-93 0.06317645383552062 1106 94-95 0.11378114047676746 1106 96-97 0.1602577579695712 1106 98-99 0.18553083131557813 1106 100 0.24379644336477213 1107 1 0.14938924939880138 1107 2 0.17353115785677176 1107 3 0.17866756526040106 1107 4 0.13453567904915076 1107 5 0.18247882356451584 1107 6 0.19763600517550373 1107 7 -12.266774826443736 1107 8 -6.735508690233747 1107 9 -2.4891239355116 1107 10-11 -0.4066423355308544 1107 12-13 0.18042350777048455 1107 14-15 0.33340922056240885 1107 16-17 0.31372921511638907 1107 18-19 0.3402725248033178 1107 20-21 0.34933335946461597 1107 22-23 0.39468210253299674 1107 24-25 0.42591359980782073 1107 26-27 0.31799968037137205 1107 28-29 0.33181196375189614 1107 30-31 0.3850119749134464 1107 32-33 0.38244726480360214 1107 34-35 0.3672423071986586 1107 36-37 0.44925886648490376 1107 38-39 0.4625436357261066 1107 40-41 0.4263351492589038 1107 42-43 0.4274297283433768 1107 44-45 0.3581156244674162 1107 46-47 0.3807894866469326 1107 48-49 0.3401489908977666 1107 50-51 0.3520310071200754 1107 52-53 0.3861041695808183 1107 54-55 0.34662857434734207 1107 56-57 0.309700384503067 1107 58-59 0.2856996823567215 1107 60-61 0.3339593690948064 1107 62-63 0.2373913468345208 1107 64-65 0.13557796048205972 1107 66-67 -10.417578304128423 1107 68-69 -8.347625645187172 1107 70-71 -6.779146122007113 1107 72-73 -1.6800842699735163 1107 74-75 -0.39026945588754103 1107 76-77 -0.13139398643561861 1107 78-79 3.103097111496268 1107 80-81 0.9602535127095173 1107 82-83 0.28569272127254663 1107 84-85 -5.488795041218236 1107 86-87 -5.940155598857107 1107 88-89 -1.4179607387183353 1107 90-91 -0.16193985531068833 1107 92-93 0.1736697353764889 1107 94-95 0.2523641202790827 1107 96-97 0.33588726537591285 1107 98-99 0.39018206984598436 1107 100 0.48046171903983037 1108 1 0.177060268379595 1108 2 0.16978205320523543 1108 3 0.20048518797510795 1108 4 0.1185687799185473 1108 5 0.12869830005144678 1108 6 0.19245664445727328 1108 7 -12.270963735763793 1108 8 -6.70298207626049 1108 9 -2.4486276065887864 1108 10-11 -0.38856286689567554 1108 12-13 0.18745470301762168 1108 14-15 0.3587562683178902 1108 16-17 0.32973620447673824 1108 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-0.727726940827985 2216 3 -0.8693184584154174 2216 4 -0.8042066729759156 2216 5 -0.6217980732800612 2216 6 -0.8850122326096042 2216 7 3.2634977136816055 2216 8 1.3308640989117322 2216 9 0.043953098439907023 2216 10-11 -0.639462524217123 2216 12-13 -1.0004219197878328 2216 14-15 -1.1588494851446995 2216 16-17 -1.3016114580228901 2216 18-19 -1.767500385632708 2216 20-21 -1.376567311969609 2216 22-23 -1.2318557430061574 2216 24-25 -1.2280540119562886 2216 26-27 -1.647717577559419 2216 28-29 -1.7971812297702527 2216 30-31 -1.6795653829249275 2216 32-33 -2.6500595820577573 2216 34-35 -1.7079754634946624 2216 36-37 -1.4562584489487733 2216 38-39 -1.6108307489891232 2216 40-41 -1.6480845547208602 2216 42-43 -1.2752010636012514 2216 44-45 -1.4396919250004743 2216 46-47 -1.60389312570139 2216 48-49 -1.6617902569541698 2216 50-51 -2.1767301954458915 2216 52-53 -1.8350049212078758 2216 54-55 -1.4830404483521349 2216 56-57 -1.5085119012011958 2216 58-59 -1.910306081602485 2216 60-61 -2.0662142847252554 2216 62-63 -1.9299847694940908 2216 64-65 -1.614717068734457 2216 66-67 1.8147753203795958 2216 68-69 0.9932948386107867 2216 70-71 0.6143048618990861 2216 72-73 -1.1607085803358643 2216 74-75 -1.9459346262165766 2216 76-77 -1.5856151651977406 2216 78-79 0.9969828262709051 2216 80-81 -0.4529317350333777 2216 82-83 -1.324844254982878 2216 84-85 0.48685080353266486 2216 86-87 0.45543059021189336 2216 88-89 -0.7273844053369878 2216 90-91 -1.0691638752298331 2216 92-93 -1.1239042026921844 2216 94-95 -1.0721776450115108 2216 96-97 -1.0876820347248888 2216 98-99 -1.0743435453652168 2216 100 -2.2433699652627865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93155.0 3 19047.0 4 11060.0 5 10277.0 6 9653.0 7 11636.0 8 14275.0 9 16356.0 10 17841.0 11 20288.0 12 22562.0 13 24851.0 14 27720.0 15 29753.0 16 30967.0 17 32203.0 18 30599.0 19 29615.0 20 29096.0 21 29589.0 22 31672.0 23 35870.0 24 42859.0 25 54577.0 26 67939.0 27 85015.0 28 107167.0 29 138819.0 30 175398.0 31 212930.0 32 280505.0 33 392272.0 34 586669.0 35 808157.0 36 886649.0 37 1490557.0 38 1706588.0 39 585694.0 40 1540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.216265234648155 20.560399927121008 14.229086375994617 22.994248462236218 2 15.62743105023321 20.486275890761192 40.26938536221825 23.616907696787344 3 18.406502593924046 25.21456099358137 30.491102548492123 25.887833864002463 4 12.798327565898731 17.095907682027796 38.70671680554844 31.399047946525027 5 13.05083824032206 37.70136618564927 35.34998661176107 13.897808962267602 6 30.493985028140674 37.903656805857835 17.996251487144622 13.606106678856872 7 27.13912978599841 32.554477500478896 22.514236271002385 17.7921564425203 8 24.70734637506022 36.3394797900217 20.840869122770098 18.112304712147985 9 24.600642327792016 17.476046830914648 22.04428013685435 35.87903070443899 10-11 23.577246379285537 27.3046435861107 29.056827963937952 20.06128207066581 12-13 24.035752345325566 24.8009247179147 29.01742625057612 22.14589668618361 14-15 21.55645607711835 27.27161272072397 26.14899005294205 25.022941149215622 16-17 21.02250578894996 30.474409189356795 26.93804387811302 21.565041143580224 18-19 20.991102760253714 29.46403183838896 28.76381772912496 20.78104767223237 20-21 22.150725773928713 28.725869643875583 28.240069475794886 20.883335106400814 22-23 21.827416583190264 28.639975067730656 28.264381550047524 21.26822679903156 24-25 21.624237022369297 28.724129480016874 28.250687076140473 21.400946421473353 26-27 21.325249031888973 28.74752199606665 28.30664046222787 21.620588509816514 28-29 21.34770564121823 28.753898715100558 28.477867247379113 21.420528396302103 30-31 21.678654428135616 29.102423726622952 28.002041109954128 21.216880735287305 32-33 21.2859523282321 28.90719114845534 28.411685250449437 21.395171272863127 34-35 21.47635282625367 28.579860044856904 28.377961920832306 21.565825208057124 36-37 21.782035281840713 28.767592468347992 28.01932520298785 21.431047046823448 38-39 21.583305568823928 28.82679731887811 28.232485420965343 21.357411691332626 40-41 21.403008345079773 28.579576986642373 28.41212426702475 21.6052904012531 42-43 21.296403041645675 28.5841206313715 28.417807877189006 21.701668449793818 44-45 21.449812986414106 28.695445581640573 28.32086972367372 21.533871708271608 46-47 21.35810348749355 28.82686914231239 28.229991689781258 21.5850356804128 48-49 21.249663699664 28.542996055112468 28.40109301190916 21.806247233314366 50-51 21.230739254634212 28.429556382840186 28.394495278608666 21.945209083916932 52-53 21.171321607034123 28.516725327426045 28.429868141928065 21.882084923611767 54-55 21.229015811783462 28.749564021728773 28.311497688164444 21.70992247832332 56-57 21.090547285520152 28.536395776520656 28.27040729543322 22.102649642525968 58-59 21.046676234150553 28.20007949871912 28.516141775967245 22.237102491163082 60-61 21.234743272408032 28.42084791435504 28.226299489105383 22.11810932413154 62-63 21.035853897005133 28.40719505629319 28.291041528056603 22.265909518645074 64-65 21.09868474437724 28.185989510120518 28.36133294664825 22.35399279885399 66-67 21.026470041013113 28.192863556573016 28.22382319869923 22.55684320371464 68-69 21.47499151290216 28.333656267540402 28.05641429546449 22.134937924092945 70-71 21.21393728087043 28.077983240985038 28.248324208243723 22.45975526990081 72-73 21.05013551457251 28.07127549524882 28.4699241537838 22.408664836394866 74-75 21.118992359881783 28.087731744033974 28.323277059331204 22.469998836753042 76-77 21.217403678320224 28.084807792565847 28.166932479545498 22.53085604956844 78-79 21.38837540863575 28.064001124070582 28.25312728810238 22.294496179191288 80-81 21.197399105599604 27.94161095716694 28.366307870052832 22.494682067180626 82-83 21.20069730227327 27.871752033234344 28.457672016865676 22.46987864762671 84-85 21.35373728924178 27.719883360234288 28.293446122123843 22.632933228400084 86-87 21.53294784535845 27.7469340927255 28.233160334615253 22.486957727300798 88-89 21.468715445542585 27.589988898678016 28.329562883236882 22.611732772542513 90-91 21.519364411321188 27.487823968166424 28.263977028595853 22.728834591916527 92-93 21.735515075590577 27.494855429223918 28.131122066658808 22.638507428526697 94-95 21.81200713028301 27.231716029492226 28.218805895859777 22.73747094436499 96-97 21.88929009933906 27.220856526968245 28.167247672263702 22.72260570142899 98-99 21.970011788869492 27.171948002159745 28.11051540820363 22.74752480076713 100 22.103492488920402 27.14908208077543 28.021317211496076 22.72610821880809 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2460.0 1 1979.0 2 1775.0 3 2026.0 4 1907.0 5 1755.0 6 1674.5 7 1653.5 8 1726.5 9 1778.0 10 1642.0 11 1484.5 12 1512.5 13 1652.5 14 1831.5 15 2049.0 16 2482.5 17 3197.5 18 4168.0 19 5582.0 20 7947.0 21 11269.5 22 16352.0 23 24243.0 24 33724.0 25 45189.0 26 60268.5 27 79291.0 28 103276.5 29 127294.0 30 147986.5 31 169132.0 32 190178.5 33 209465.5 34 227728.0 35 245748.5 36 261909.0 37 275298.0 38 285705.5 39 292168.5 40 297390.5 41 299021.0 42 296993.5 43 295991.0 44 292332.5 45 285939.5 46 280268.5 47 273415.5 48 266173.0 49 262009.0 50 255754.0 51 244431.5 52 231660.5 53 220848.0 54 211480.5 55 199268.5 56 181217.5 57 160301.5 58 141375.5 59 124707.0 60 106802.0 61 88335.5 62 72597.0 63 58628.0 64 46578.0 65 36655.5 66 28743.0 67 22231.0 68 17028.0 69 12790.5 70 9226.5 71 6673.0 72 4979.5 73 3586.0 74 2507.0 75 1705.5 76 1129.0 77 809.0 78 608.0 79 484.5 80 341.0 81 214.5 82 159.5 83 120.5 84 95.0 85 82.0 86 65.0 87 45.5 88 34.0 89 25.0 90 27.0 91 27.0 92 23.5 93 22.0 94 21.5 95 20.0 96 20.5 97 19.5 98 16.0 99 26.0 100 38.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018606533990455313 2 0.0028287784310521836 3 0.0016948284565355756 4 0.002438602095734641 5 0.0023410580119052555 6 0.0036700961540806344 7 11.588224965920537 8 1.2193010478673205E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.316671990947909E-4 18-19 0.0 20-21 3.048252619668301E-5 22-23 6.0965052393366025E-6 24-25 0.0 26-27 1.2802661002606866E-4 28-29 0.0 30-31 0.0 32-33 4.877204191469282E-5 34-35 7.925456811137584E-5 36-37 6.706155763270263E-5 38-39 3.048252619668301E-5 40-41 6.0965052393366025E-6 42-43 5.486854715402942E-5 44-45 1.2802661002606866E-4 46-47 8.718002492251342E-4 48-49 5.547819767796308E-4 50-51 2.9263225148815693E-4 52-53 3.1701827244550335E-4 54-55 4.4504488247157196E-4 56-57 0.0015241263098341507 58-59 3.6579031436019614E-4 60-61 0.0014631612574407846 62-63 0.0025056636533673437 64-65 0.0010546954064052324 66-67 5.7945148523060634 68-69 5.450306166493119 70-71 0.0027068483262654517 72-73 0.001072984922123242 74-75 0.0029019364939242226 76-77 0.005797776482609109 78-79 5.4124773514830355 80-81 0.0048162391390759165 82-83 0.00470040553952852 84-85 5.794588010368936 86-87 0.003651806638362625 88-89 0.0017618900141682782 90-91 0.0032555337978057456 92-93 0.003639613627883952 94-95 0.004182202594184909 96-97 0.0032372442820877358 98-99 0.005657556862104367 100 0.0065232606060901645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8201420.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.33541650957606 #Duplication Level Percentage of deduplicated Percentage of total 1 91.20610024394654 78.74316652775272 2 6.099694405155613 10.532393141004812 3 1.3826444620339442 3.5811355654307784 4 0.5369540125226694 1.8543259327053117 5 0.2738657571010719 1.1822157103515716 6 0.15768388214640308 0.8168222185173968 7 0.09598479786687354 0.5800821251696796 8 0.06433327955083755 0.44433923883588416 9 0.042184583306912 0.3277821213076641 >10 0.13837811434990166 1.795221936959738 >50 0.0018500410405608144 0.10032479627063721 >100 3.1161600637110657E-4 0.035007238539812566 >500 1.480497222862369E-5 0.0071834471539926415 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2193010478673205E-5 0.0 3 0.0 0.0 0.0 1.2193010478673205E-5 0.0 4 0.0 0.0 0.0 1.2193010478673205E-5 0.0 5 0.0 0.0 0.0 1.2193010478673205E-5 0.0 6 0.0 0.0 0.0 3.6579031436019616E-5 0.0 7 0.0 0.0 0.0 4.877204191469282E-5 0.0 8 0.0 0.0 0.0 4.877204191469282E-5 0.0 9 0.0 0.0 0.0 9.754408382938564E-5 0.0 10-11 0.0 0.0 0.0 1.2802661002606866E-4 0.0 12-13 0.0 0.0 0.0 1.7679865194076148E-4 0.0 14-15 0.0 0.0 0.0 2.072811781374445E-4 0.0 16-17 0.0 0.0 0.0 2.072811781374445E-4 0.0 18-19 0.0 1.2193010478673205E-5 0.0 2.316671990947909E-4 0.0 20-21 0.0 1.2193010478673205E-5 0.0 3.3530778816351315E-4 0.0 22-23 0.0 1.2193010478673205E-5 0.0 7.803526706350851E-4 0.0 24-25 0.0 1.2193010478673205E-5 0.0 0.0016338634041422094 0.0 26-27 0.0 1.2193010478673205E-5 0.0 0.0033043058397204386 0.0 28-29 0.0 1.2193010478673205E-5 0.0 0.009870241982485959 0.0 30-31 0.0 1.2193010478673205E-5 0.0 0.030689807374820455 0.0 32-33 0.0 2.438602095734641E-5 0.0 0.08180900380665787 0.0 34-35 0.0 2.438602095734641E-5 0.0 0.1707387257328609 0.0 36-37 0.0 3.048252619668301E-5 0.0 0.3146650214231194 0.0 38-39 0.0 3.6579031436019616E-5 0.0 0.5149047360091301 0.0 40-41 0.0 3.6579031436019616E-5 0.0 0.7276849618724562 0.0 42-43 6.0965052393366025E-6 3.6579031436019616E-5 0.0 0.9513718356089556 0.0 44-45 1.2193010478673205E-5 3.6579031436019616E-5 0.0 1.2265741786178492 0.0 46-47 1.2193010478673205E-5 3.6579031436019616E-5 0.0 1.5643144723718576 0.0 48-49 1.2193010478673205E-5 3.6579031436019616E-5 0.0 1.9234108239792622 0.0 50-51 1.2193010478673205E-5 3.6579031436019616E-5 0.0 2.2837020906135765 0.0 52-53 1.2193010478673205E-5 3.6579031436019616E-5 0.0 2.643414189250154 0.0 54-55 1.2193010478673205E-5 3.6579031436019616E-5 0.0 3.028865733007211 0.0 56-57 1.2193010478673205E-5 3.6579031436019616E-5 0.0 3.443556115892126 0.0 58-59 1.2193010478673205E-5 3.6579031436019616E-5 0.0 3.890320212841191 0.0 60-61 1.2193010478673205E-5 3.6579031436019616E-5 0.0 4.344887836496607 0.0 62-63 1.2193010478673205E-5 3.6579031436019616E-5 0.0 4.78468972446235 0.0 64-65 1.2193010478673205E-5 4.267553667535622E-5 0.0 5.259753798732415 0.0 66-67 1.2193010478673205E-5 4.877204191469282E-5 0.0 5.780724557454684 0.0 68-69 1.2193010478673205E-5 4.877204191469282E-5 0.0 6.2821133413482055 0.0 70-71 1.2193010478673205E-5 4.877204191469282E-5 0.0 6.861006996349413 0.0 72-73 1.2193010478673205E-5 4.877204191469282E-5 0.0 7.469547956329514 0.0 74-75 1.2193010478673205E-5 7.315806287203923E-5 0.0 8.005132038110474 0.0 76-77 1.2193010478673205E-5 7.315806287203923E-5 0.0 8.55287255133867 0.0 78-79 1.2193010478673205E-5 7.315806287203923E-5 0.0 9.174807533329595 0.0 80-81 1.2193010478673205E-5 7.315806287203923E-5 0.0 9.895640023313035 0.0 82-83 1.2193010478673205E-5 7.315806287203923E-5 0.0 10.607864247898533 0.0 84-85 1.2193010478673205E-5 7.315806287203923E-5 0.0 11.29714732326841 0.0 86-87 1.2193010478673205E-5 7.925456811137584E-5 0.0 12.081852898644382 0.0 88 1.2193010478673205E-5 1.0973709430805885E-4 0.0 12.716736857763655 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7565 0.0 41.8791 1 GTATCAA 12320 0.0 35.4783 1 TCAACGC 12795 0.0 32.754604 4 ATCAACG 12890 0.0 32.592598 3 CAACGCA 14135 0.0 29.721867 5 TATCAAC 14805 0.0 28.826134 2 AACGCAG 15615 0.0 26.80576 6 GTACATG 22760 0.0 25.928219 1 TACATGG 23215 0.0 24.753681 2 ACATGGG 23690 0.0 23.306932 3 ACGCAGA 18465 0.0 22.55677 7 CGCAGAG 18530 0.0 22.461054 8 GTGGTAT 4855 0.0 21.189583 1 CATGGGG 15505 0.0 19.603943 4 GCAGAGT 21330 0.0 18.409695 9 GAGTACA 17380 0.0 17.463068 1 TGGTATC 5370 0.0 17.343065 2 AGAGTAC 18465 0.0 16.317087 10-11 CATGGGT 6705 0.0 16.17953 4 TACCTGG 4960 0.0 15.991115 2 >>END_MODULE