FastQCFastQC Report
Wed 25 May 2016
SRR2049529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8201420
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230640.28121959368011884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227670.27759826956795286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131770.16066729907747682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149700.056.167551
GTATCAA262450.039.4282041
ATCAACG328450.031.187393
TCAACGC331400.030.980764
GTGGTAT60650.030.5837861
TATCAAC338050.030.3852372
CAACGCA342000.029.9716555
AACGCAG361400.028.4401076
TGGTATC67300.025.8688142
ACGCAGA419450.024.392087
CGCAGAG416800.024.366758
GTACATG233150.023.5040871
GCAGAGT451550.022.0544249
TACATGG237950.022.0287232
GAGTACT316150.021.10147512-13
ACATGGG242550.021.0289633
CAGAGTA439350.020.19049310-11
AGAGTAC416100.019.78258510-11
GTACTTT346800.019.01971214-15
AGTACTT326950.018.7873212-13
Produced by FastQC (version 0.11.5)