##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8201420 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67407973253412 31.0 31.0 34.0 30.0 34.0 2 31.80470272221152 31.0 31.0 34.0 30.0 34.0 3 31.916182075786875 33.0 31.0 34.0 30.0 34.0 4 35.60213670315628 37.0 35.0 37.0 33.0 37.0 5 35.69338712076689 37.0 35.0 37.0 33.0 37.0 6 35.792243538314096 37.0 35.0 37.0 35.0 37.0 7 35.786769973980114 37.0 35.0 37.0 35.0 37.0 8 35.785922559751846 37.0 35.0 37.0 33.0 37.0 9 37.52239331725482 39.0 37.0 39.0 35.0 39.0 10-11 37.47611163676534 39.0 37.0 39.0 34.5 39.0 12-13 37.459802753669486 39.0 37.0 39.0 34.0 39.0 14-15 38.72821005386872 40.0 38.0 41.0 34.5 41.0 16-17 38.73343610008999 40.0 38.0 41.0 34.5 41.0 18-19 38.80522257121328 40.0 38.0 41.0 35.0 41.0 20-21 38.832437675426945 40.0 38.0 41.0 35.0 41.0 22-23 38.8075997205362 40.0 38.0 41.0 35.0 41.0 24-25 38.769140831709635 40.0 38.0 41.0 34.0 41.0 26-27 38.67985495194735 40.0 38.0 41.0 34.0 41.0 28-29 38.59244831992508 40.0 38.0 41.0 34.0 41.0 30-31 38.48226453467814 40.0 38.0 41.0 34.0 41.0 32-33 38.34600526494192 40.0 38.0 41.0 34.0 41.0 34-35 38.2426703546459 40.0 38.0 41.0 34.0 41.0 36-37 38.105399552760375 40.0 38.0 41.0 33.5 41.0 38-39 38.01639777014225 40.0 38.0 41.0 33.0 41.0 40-41 37.9016391673637 40.0 37.0 41.0 33.0 41.0 42-43 37.761725469491864 40.0 37.0 41.0 33.0 41.0 44-45 37.58631730846609 40.0 37.0 41.0 33.0 41.0 46-47 37.351991728261694 40.0 37.0 41.0 32.5 41.0 48-49 37.1316761609575 40.0 37.0 41.0 31.5 41.0 50-51 36.92488264227414 40.0 36.5 41.0 31.0 41.0 52-53 36.823151905401744 40.0 36.0 41.0 31.0 41.0 54-55 36.69532373662122 39.5 36.0 41.0 31.0 41.0 56-57 36.52858828105377 39.0 35.5 41.0 31.0 41.0 58-59 36.35915384165181 39.0 35.0 41.0 30.5 41.0 60-61 36.172484155182886 39.0 35.0 41.0 30.0 41.0 62-63 35.97584448790575 39.0 35.0 41.0 30.0 41.0 64-65 35.69923123068932 38.0 35.0 40.0 29.5 41.0 66-67 35.44296073119045 37.5 35.0 40.0 29.0 41.0 68-69 35.13066682355007 37.0 34.5 40.0 29.0 41.0 70-71 34.83572119218379 37.0 34.0 39.5 29.0 41.0 72-73 34.514221378736856 36.0 34.0 39.0 29.0 41.0 74-75 34.177637896852005 36.0 34.0 39.0 28.0 40.5 76-77 33.87877574858988 35.0 34.0 38.5 28.0 40.0 78-79 33.548384426111575 35.0 34.0 37.0 28.0 39.0 80-81 33.23708071285216 35.0 34.0 37.0 28.0 39.0 82-83 32.939350807543086 35.0 34.0 36.0 27.0 39.0 84-85 32.66458332825291 35.0 33.0 36.0 27.0 37.0 86-87 32.38410324065832 35.0 33.0 36.0 26.0 37.0 88-89 32.17231607453344 35.0 33.0 35.0 26.0 37.0 90-91 32.01749014195103 35.0 33.0 35.0 26.0 36.0 92-93 31.85807988128885 35.0 33.0 35.0 26.0 36.0 94-95 31.67107616729786 35.0 33.0 35.0 25.5 36.0 96-97 31.50050210817151 35.0 33.0 35.0 25.0 35.5 98-99 31.31369787183195 35.0 33.0 35.0 25.0 35.0 100 31.197691985046493 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34356637939851353 1101 2 0.4412249523118845 1101 3 0.4657734701634233 1101 4 0.37092794473080914 1101 5 0.30459247085443053 1101 6 0.29382445208316454 1101 7 0.3358137404635926 1101 8 0.3227601009124399 1101 9 0.33827314620300086 1101 10-11 0.369756619922029 1101 12-13 0.34725147515688093 1101 14-15 0.4856814816233239 1101 16-17 0.46358366448335886 1101 18-19 0.46666865758106724 1101 20-21 0.4271060418512178 1101 22-23 0.46760266393523864 1101 24-25 0.49400203216257665 1101 26-27 0.48334078042396555 1101 28-29 0.4358697733968171 1101 30-31 0.47245347408981075 1101 32-33 0.5266431735987211 1101 34-35 0.5335293264490417 1101 36-37 0.508017188542766 1101 38-39 0.5386525345842514 1101 40-41 0.5803743188448038 1101 42-43 0.45838494258155293 1101 44-45 0.4783343045169701 1101 46-47 0.5868397705649784 1101 48-49 0.590916044575593 1101 50-51 0.6266005335875207 1101 52-53 0.6102791790114921 1101 54-55 0.5187260484608842 1101 56-57 0.518544727996705 1101 58-59 0.5247336516712409 1101 60-61 0.5664469105589518 1101 62-63 0.588785830454114 1101 64-65 0.529118823762154 1101 66-67 0.5442755331386948 1101 68-69 0.4960060904272652 1101 70-71 0.5033113716970874 1101 72-73 0.489507207814043 1101 74-75 0.4822782645881887 1101 76-77 0.4686878094538116 1101 78-79 0.444973890066656 1101 80-81 0.4448455428043374 1101 82-83 0.4979726989956532 1101 84-85 0.49637415126425566 1101 86-87 0.5994235390013358 1101 88-89 0.5804674345995764 1101 90-91 0.5434275523570733 1101 92-93 0.5624874060594927 1101 94-95 0.5193418988996008 1101 96-97 0.5731526529788873 1101 98-99 0.6274015206520858 1101 100 0.6934502664042554 1102 1 0.23226357708563228 1102 2 0.337565676450609 1102 3 0.373536908240947 1102 4 0.34429079755176417 1102 5 0.30332015582806093 1102 6 0.2546376310856999 1102 7 0.2738770206124599 1102 8 0.2945133684382313 1102 9 0.3689849629811164 1102 10-11 0.3386709103273873 1102 12-13 0.3060354499824314 1102 14-15 0.39223796237114783 1102 16-17 0.44845083860279544 1102 18-19 0.43091651499987194 1102 20-21 0.3879491142110254 1102 22-23 0.40109889695526135 1102 24-25 0.3907773044886298 1102 26-27 0.3602525480662493 1102 28-29 0.369931814355418 1102 30-31 0.47225199216687486 1102 32-33 0.46709305785432775 1102 34-35 0.4540101932410465 1102 36-37 0.5172975529317867 1102 38-39 0.5012654535273171 1102 40-41 0.5709368319122916 1102 42-43 0.5866990573005992 1102 44-45 0.6386544411781401 1102 46-47 0.6505255124134024 1102 48-49 0.7033309122088269 1102 50-51 0.769069100855539 1102 52-53 0.6616997377562157 1102 54-55 0.6734311795045116 1102 56-57 0.6527346189412029 1102 58-59 0.6704688529073977 1102 60-61 0.702833876397321 1102 62-63 0.6915799941774239 1102 64-65 0.699614712516329 1102 66-67 0.7031108814534051 1102 68-69 0.6846471196427046 1102 70-71 0.6935816641673966 1102 72-73 0.6981672311829783 1102 74-75 0.6981343869446519 1102 76-77 0.6942787655700897 1102 78-79 0.6319284057889405 1102 80-81 0.6167599240595578 1102 82-83 0.5880616646845596 1102 84-85 0.5750570117219738 1102 86-87 0.5445463885385351 1102 88-89 0.5499579336544116 1102 90-91 0.5497902972793298 1102 92-93 0.5770338194751083 1102 94-95 0.6199329302994165 1102 96-97 0.5949467172352634 1102 98-99 0.6543960098504726 1102 100 0.7132370513675426 1103 1 0.24326550114873058 1103 2 0.3079572909043904 1103 3 0.41347098190196974 1103 4 0.3483514754763277 1103 5 0.2579757625952439 1103 6 0.2854565440751031 1103 7 0.23362689747426657 1103 8 0.2693125451157101 1103 9 0.30517290965218535 1103 10-11 0.29354338122773527 1103 12-13 0.25285419309729207 1103 14-15 0.35716863302971547 1103 16-17 0.3480282138515918 1103 18-19 0.3577560065238359 1103 20-21 0.3742195969118427 1103 22-23 0.36322153571173743 1103 24-25 0.36845204430753853 1103 26-27 0.36984506008445095 1103 28-29 0.40987983071855183 1103 30-31 0.4238204448609153 1103 32-33 0.4202982139608977 1103 34-35 0.388777935679542 1103 36-37 0.42269699689801854 1103 38-39 0.43569355114661334 1103 40-41 0.449521033633161 1103 42-43 0.42415724818673795 1103 44-45 0.4682090564553434 1103 46-47 0.5606464751855853 1103 48-49 0.5132316949872759 1103 50-51 0.5072554985162441 1103 52-53 0.47832542390175803 1103 54-55 0.48571550891729487 1103 56-57 0.5379907313339132 1103 58-59 0.4886373079788413 1103 60-61 0.47276537870232005 1103 62-63 0.4998152720320874 1103 64-65 0.48843618536201205 1103 66-67 0.47194745710550734 1103 68-69 0.4697656208427432 1103 70-71 0.4682010499991094 1103 72-73 0.4397070423493332 1103 74-75 0.46525965232149247 1103 76-77 0.4722254372370571 1103 78-79 0.42139125782764353 1103 80-81 0.3401421587392477 1103 82-83 0.4052969641980866 1103 84-85 0.43235614438162884 1103 86-87 0.4881770568465029 1103 88-89 0.44920534651601685 1103 90-91 0.4586661439819437 1103 92-93 0.4892110775754084 1103 94-95 0.4529526422116099 1103 96-97 0.42349082921876047 1103 98-99 0.49099040863206866 1103 100 0.5053852067584792 1104 1 0.20209092411966978 1104 2 0.24199637970893662 1104 3 0.3332559202081917 1104 4 0.25569499079387015 1104 5 0.2032395773248652 1104 6 0.23071316555441967 1104 7 0.19276835433377215 1104 8 0.1967046015098859 1104 9 0.25668319510452875 1104 10-11 0.25561083480944546 1104 12-13 0.27011727593502144 1104 14-15 0.35551046799830033 1104 16-17 0.3182106062476038 1104 18-19 0.30975886613936154 1104 20-21 0.34265078825539774 1104 22-23 0.34800540171188743 1104 24-25 0.30899684516410275 1104 26-27 0.32952539232504563 1104 28-29 0.3203419185790679 1104 30-31 0.3866724744990293 1104 32-33 0.33265340608451055 1104 34-35 0.3382056187924647 1104 36-37 0.3133286157367223 1104 38-39 0.3361967758693538 1104 40-41 0.33923996263722955 1104 42-43 0.33069922786759776 1104 44-45 0.35164241387705886 1104 46-47 0.40525481848592904 1104 48-49 0.3631090159513306 1104 50-51 0.3396927414588333 1104 52-53 0.36521820873336 1104 54-55 0.27168691372865794 1104 56-57 0.24502605852345738 1104 58-59 0.3015485937933704 1104 60-61 0.3011269203335729 1104 62-63 0.29807635921198283 1104 64-65 0.34494863291205746 1104 66-67 0.32290798063311144 1104 68-69 0.23626512452269566 1104 70-71 0.2402655705543424 1104 72-73 0.27915642491115733 1104 74-75 0.35172782214623055 1104 76-77 0.30419570886982683 1104 78-79 0.30260449530746314 1104 80-81 0.2874126486345645 1104 82-83 0.2982239122489716 1104 84-85 0.2884010306615963 1104 86-87 0.2903632562062697 1104 88-89 0.2584418552125598 1104 90-91 0.29585618490699517 1104 92-93 0.3389680608638379 1104 94-95 0.33674445379644524 1104 96-97 0.3281225176016953 1104 98-99 0.3790919651576097 1104 100 0.4457813944950324 1105 1 0.1766780990412542 1105 2 0.20837904369081883 1105 3 0.2981843879067334 1105 4 0.21026031457775218 1105 5 0.17866010288283007 1105 6 0.21031058847598416 1105 7 0.18608072447347723 1105 8 0.20663170178088563 1105 9 0.2178356099948786 1105 10-11 0.19583834911379938 1105 12-13 0.21205318858022792 1105 14-15 0.26282531722934976 1105 16-17 0.26285130469636897 1105 18-19 0.2702557557254863 1105 20-21 0.2883369485130203 1105 22-23 0.25217426453052383 1105 24-25 0.2535102728354346 1105 26-27 0.27465234607984 1105 28-29 0.3189266898048899 1105 30-31 0.30724387140215015 1105 32-33 0.32685134495246615 1105 34-35 0.27307513114155313 1105 36-37 0.32447820532988914 1105 38-39 0.3021928168410355 1105 40-41 0.337292497032017 1105 42-43 0.29171983629552045 1105 44-45 0.3213687909398786 1105 46-47 0.29127928122758817 1105 48-49 0.3345319021567903 1105 50-51 0.3612331032633591 1105 52-53 0.34225780068684486 1105 54-55 0.31233535929034417 1105 56-57 0.2785976676395947 1105 58-59 0.29276976532250387 1105 60-61 0.30644972659555236 1105 62-63 0.3003835494500535 1105 64-65 0.3032297622992459 1105 66-67 0.3307660956070464 1105 68-69 0.3261483660778808 1105 70-71 0.29767727974561353 1105 72-73 0.2878618812084639 1105 74-75 0.2998205638946061 1105 76-77 0.2873627973529409 1105 78-79 0.23871298636640859 1105 80-81 0.23743487281198838 1105 82-83 0.22810456153798242 1105 84-85 0.24603477928668127 1105 86-87 0.3571208156009007 1105 88-89 0.25812959910323485 1105 90-91 0.24859644547068882 1105 92-93 0.2805894257924102 1105 94-95 0.2717156252407058 1105 96-97 0.2943323163081324 1105 98-99 0.3490725273030755 1105 100 0.37647809702896495 1106 1 0.10791434840926684 1106 2 0.18130971712134425 1106 3 0.2757403142039472 1106 4 0.2203726095699281 1106 5 0.14345927359429567 1106 6 0.14373290058897936 1106 7 0.16729484613301793 1106 8 0.14963069998590584 1106 9 0.1581601482418975 1106 10-11 0.16798489077559253 1106 12-13 0.14480727348608013 1106 14-15 0.21834739467600883 1106 16-17 0.24680858570570763 1106 18-19 0.21895035778987904 1106 20-21 0.17520725970295103 1106 22-23 0.16289621139033983 1106 24-25 0.19748944471272267 1106 26-27 0.2222174945583788 1106 28-29 0.2537744116493812 1106 30-31 0.21762024226817545 1106 32-33 0.24777857636163247 1106 34-35 0.19278359193471317 1106 36-37 0.1920433943463422 1106 38-39 0.2535985780641923 1106 40-41 0.201917966665782 1106 42-43 0.1961255808863953 1106 44-45 0.23824530651715747 1106 46-47 0.1697563203149599 1106 48-49 0.13645226099377084 1106 50-51 0.17114163198816357 1106 52-53 0.18394533786507594 1106 54-55 0.11780950227569065 1106 56-57 0.15197777365133192 1106 58-59 0.1324055590912181 1106 60-61 0.12117282092616932 1106 62-63 0.11823409870368806 1106 64-65 0.07126550662994902 1106 66-67 0.10318359155443346 1106 68-69 0.14806689236624493 1106 70-71 0.1310852041556032 1106 72-73 0.1430356642744428 1106 74-75 0.13945897763822757 1106 76-77 0.14146534632521934 1106 78-79 0.1391973421703625 1106 80-81 0.09131430028933352 1106 82-83 0.12367080407111786 1106 84-85 0.14115420530310274 1106 86-87 0.20513160682133247 1106 88-89 0.17407197305494293 1106 90-91 0.14260262981066063 1106 92-93 0.15224763476296133 1106 94-95 0.2106083667527301 1106 96-97 0.2387152738953553 1106 98-99 0.2619139980177785 1106 100 0.3128561318788563 1107 1 0.1541305012004095 1107 2 0.1848715839842754 1107 3 0.2348713370910822 1107 4 0.2000470870958253 1107 5 0.1522006818622046 1107 6 0.16554049875129095 1107 7 0.14762547905550605 1107 8 0.15786197653877565 1107 9 0.140418689447813 1107 10-11 0.1085715529009974 1107 12-13 0.10806732712904932 1107 14-15 0.16550390497331335 1107 16-17 0.1713455407307123 1107 18-19 0.18368712251993458 1107 20-21 0.247266788665101 1107 22-23 0.21020388929876788 1107 24-25 0.21990095999262138 1107 26-27 0.2125314979783255 1107 28-29 0.23538493947742722 1107 30-31 0.19567563751980543 1107 32-33 0.194916900280667 1107 34-35 0.19468490549343898 1107 36-37 0.2110847606714472 1107 38-39 0.1764314922737924 1107 40-41 0.17897706798171242 1107 42-43 0.17231999220474137 1107 44-45 0.22865709025539616 1107 46-47 0.2445969954663667 1107 48-49 0.24129753067873594 1107 50-51 0.3086950268903763 1107 52-53 0.3092626863611301 1107 54-55 0.3033986770128507 1107 56-57 0.2558248851065983 1107 58-59 0.25838168821420027 1107 60-61 0.25983204654866654 1107 62-63 0.2581829592167466 1107 64-65 0.2571492398201016 1107 66-67 0.27001621843447055 1107 68-69 0.34968131010081294 1107 70-71 0.3509440595977935 1107 72-73 0.3342671057983111 1107 74-75 0.30135480180227603 1107 76-77 0.258978531829932 1107 78-79 0.19179017944625798 1107 80-81 0.21968855830032652 1107 82-83 0.2702836025331692 1107 84-85 0.27881420738502527 1107 86-87 0.34107409951482026 1107 88-89 0.3226213544745775 1107 90-91 0.2510194682967466 1107 92-93 0.21906788881829797 1107 94-95 0.23327939936767805 1107 96-97 0.28114812735180195 1107 98-99 0.3075074005171814 1107 100 0.2755998521757057 1108 1 0.09794270787356396 1108 2 0.13775286879595683 1108 3 0.22714283169048244 1108 4 0.2014901126430999 1108 5 0.1496183513785425 1108 6 0.1719352616012486 1108 7 0.15772502458078463 1108 8 0.16561015584841954 1108 9 0.12468951053135413 1108 10-11 0.15053841372315446 1108 12-13 0.1763913238933199 1108 14-15 0.18519860154075474 1108 16-17 0.19743247594846736 1108 18-19 0.1783523379202805 1108 20-21 0.17143752968696901 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28-29 -0.5891176945703123 2215 30-31 -0.5666589405626183 2215 32-33 -0.668594578170449 2215 34-35 -0.7309604079752674 2215 36-37 -0.7792103822638126 2215 38-39 -0.7323446616943912 2215 40-41 -0.7404384083762423 2215 42-43 -0.7485565132279461 2215 44-45 -0.8273334336734877 2215 46-47 -0.7796587146337828 2215 48-49 -0.7134500416261815 2215 50-51 -0.6886974356613678 2215 52-53 -0.8769584909081587 2215 54-55 -0.790025944093685 2215 56-57 -0.7249521120055817 2215 58-59 -0.7349468010985234 2215 60-61 -0.9049394176793868 2215 62-63 -0.8608855987762638 2215 64-65 -0.7598185981233243 2215 66-67 -0.6742850167498133 2215 68-69 -0.7020275520381958 2215 70-71 -0.7438844453046016 2215 72-73 -0.62489942175106 2215 74-75 -0.7369054551607874 2215 76-77 -0.7263590519269343 2215 78-79 -0.6925986333050389 2215 80-81 -0.7198416156976535 2215 82-83 -0.6706978191174571 2215 84-85 -0.6495986508740117 2215 86-87 -0.6710352757745426 2215 88-89 -0.6620584721386855 2215 90-91 -0.6066395406444016 2215 92-93 -0.7476779866034882 2215 94-95 -0.805568646425403 2215 96-97 -0.8749741859474511 2215 98-99 -0.861314961382778 2215 100 -0.9645008043255103 2216 1 -0.5875872548133927 2216 2 -1.0630679138110573 2216 3 -0.6956341860742015 2216 4 -1.1742679093254367 2216 5 -0.6262615249322465 2216 6 -0.8344142285266614 2216 7 -0.5838645044941799 2216 8 -0.5197374235495928 2216 9 -0.6026406699855329 2216 10-11 -0.8093622159666083 2216 12-13 -0.663794179092875 2216 14-15 -0.914847522425184 2216 16-17 -0.8597883292487296 2216 18-19 -0.8810082025036792 2216 20-21 -0.7307555179823453 2216 22-23 -0.7332272274180056 2216 24-25 -0.8447798308851588 2216 26-27 -0.9254804460219148 2216 28-29 -1.1463694154083512 2216 30-31 -1.2519242157704653 2216 32-33 -1.0267082342706928 2216 34-35 -0.9977720911761239 2216 36-37 -0.9912022849289741 2216 38-39 -1.1132297945457168 2216 40-41 -0.9659747897295574 2216 42-43 -1.598345468629205 2216 44-45 -1.271726211868831 2216 46-47 -1.139323323925744 2216 48-49 -1.0718193783945793 2216 50-51 -1.2394245008805953 2216 52-53 -1.1862884884404963 2216 54-55 -1.3491311193804663 2216 56-57 -1.4417364329683053 2216 58-59 -1.1922615718974185 2216 60-61 -1.3937449856346547 2216 62-63 -1.3701077505904706 2216 64-65 -1.355375434406767 2216 66-67 -1.216692555898483 2216 68-69 -1.5354257182928919 2216 70-71 -1.3333521580781138 2216 72-73 -1.1282876566914979 2216 74-75 -1.2139612003735607 2216 76-77 -1.3385731389423725 2216 78-79 -1.376340695460783 2216 80-81 -1.173999386474506 2216 82-83 -1.2112847894549752 2216 84-85 -1.3282830566259562 2216 86-87 -1.4712122983830653 2216 88-89 -1.3093675671063991 2216 90-91 -1.179281268486836 2216 92-93 -1.2624046798638595 2216 94-95 -1.332953148175978 2216 96-97 -1.399197561744007 2216 98-99 -1.3777512125796214 2216 100 -1.3947227381008922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 570.0 3 56.0 4 60.0 5 155.0 6 445.0 7 1142.0 8 2581.0 9 4436.0 10 6532.0 11 9354.0 12 13836.0 13 18994.0 14 22020.0 15 28243.0 16 37039.0 17 51764.0 18 51802.0 19 29112.0 20 21787.0 21 21385.0 22 22584.0 23 24662.0 24 28667.0 25 35413.0 26 44932.0 27 58230.0 28 76412.0 29 100671.0 30 130782.0 31 167866.0 32 217612.0 33 283439.0 34 384364.0 35 556020.0 36 907443.0 37 1657219.0 38 2290347.0 39 890381.0 40 3063.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08314306914978 20.98664576017709 14.707573893143145 23.22263727752999 2 15.603305144233353 20.87665064866388 39.327616990129854 24.192427216972913 3 17.840817283060076 25.31124495788411 31.221235157139898 25.626702601915913 4 12.791128335852509 17.571608506715346 37.984058270712026 31.653204886720115 5 12.556732205456486 37.59558603871361 35.48906569120561 14.358616064624293 6 30.099362659685635 38.33829394715696 17.441786455024648 14.120556938132752 7 26.28222917494775 33.06338414567234 22.519856317564518 18.134530361815393 8 24.859231694023716 36.06679087280008 20.480185138671107 18.5937922945051 9 24.514873466377825 16.841955481595434 22.04508074641248 36.598090305614264 10-11 23.577112256170274 27.156065656922824 28.73775516922679 20.529066917680108 12-13 24.430249474526878 24.626849611442218 29.148116267935848 21.794784646095064 14-15 21.67002309783081 26.755862201659113 26.9652649763861 24.608849724123978 16-17 20.468839542420703 30.00950445166812 27.803599864413727 21.71805614149745 18-19 20.33615520239178 28.199415467077653 30.79565489878582 20.668774431744747 20-21 21.44731644032375 27.42059911576288 30.646558766652603 20.485525677260767 22-23 21.096115063001285 27.35056856007862 30.71321185843427 20.840104518485823 24-25 20.951573020281852 27.388683910834956 30.72221639667277 20.93752667221042 26-27 20.700707926188393 27.383879864706355 30.772201643130092 21.14321056597516 28-29 20.739307066669454 27.41188572525247 30.933054860874833 20.915752347203245 30-31 21.087360230477625 27.70963530815183 30.460505936575366 20.742498524795177 32-33 20.723500453000902 27.54127228113039 30.841280688212652 20.89394657765605 34-35 20.89885044297207 27.27083846455858 30.749821372396486 21.080489720072865 36-37 21.251454016499583 27.431688658793234 30.295241555730595 21.02161576897659 38-39 21.071479085329127 27.511345596250404 30.48598901165896 20.93118630676151 40-41 20.922090320944424 27.31214838406032 30.549727973936218 21.216033321059037 42-43 20.91594504366317 27.319055724496486 30.421408731658662 21.343590500181676 44-45 21.064023059421416 27.499195261308408 30.202154017231166 21.234627662039014 46-47 21.122092271826098 27.694941851533027 29.798059360452218 21.38490651618866 48-49 21.123342055400162 27.56084312228858 29.65943702432018 21.65637779799108 50-51 21.15083119752433 27.59379473310719 29.426422497567494 21.82895157180098 52-53 21.02546876028785 27.71440189625699 29.406913680801615 21.85321566265354 54-55 21.114057077920652 27.97537499603727 29.26860836294203 21.64195956310005 56-57 20.892089418661648 27.719151073838432 29.274393946414158 22.114365561085762 58-59 20.798141196765528 27.38646276016576 29.571874627350482 22.24352141571823 60-61 21.019524667679498 27.610517446978694 29.275314518705297 22.09464336663651 62-63 20.73592743695604 27.56643971409829 29.367036440031118 22.330596408914555 64-65 20.694818702127193 27.37326584908467 29.47336558791039 22.45854986087775 66-67 20.64282160894089 27.362651833462987 29.30000536492461 22.694521192671512 68-69 20.929920785659164 27.546647720351807 29.030945742437986 22.49248575155104 70-71 20.843799000660862 27.28585415696306 29.041733016965352 22.82861382541072 72-73 20.626952405802896 27.306698108376352 29.157865345269478 22.90848414055127 74-75 20.678961691999678 27.32384148110937 28.948986882759325 23.048209944131624 76-77 20.725496316491533 27.339320507912046 28.766427033367393 23.16875614222903 78-79 20.761464094410094 27.33810072437535 28.7846018203453 23.115833360869246 80-81 20.591934628686058 27.207802623346232 28.773597127464466 23.426665620503243 82-83 20.428828240267162 27.118433686072084 28.920316060845984 23.53242201281477 84-85 20.46785190857193 26.98383938391157 28.750423707114138 23.79788500040237 86-87 20.625441729934675 27.088633863930507 28.621770901976173 23.664153504158637 88-89 20.52076504607833 26.908961862615282 28.633877729262437 23.93639536204395 90-91 20.327091137754348 26.770461363707444 28.600889517289975 24.301557981248234 92-93 20.353009443425712 26.768952053968565 28.55743438868179 24.320604113923935 94-95 20.27119397973166 26.537634846504766 28.73590996227212 24.455261211491457 96-97 20.285366453941297 26.539064365625975 28.65164987597574 24.52391930445699 98-99 20.281007538087625 26.505529471309654 28.546256092087503 24.66720689851522 100 20.27176524625296 26.46687255977466 28.461594643706523 24.79976755026585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1048.0 1 839.5 2 1574.0 3 2496.5 4 1649.0 5 871.5 6 1869.5 7 4636.5 8 7449.0 9 8642.0 10 7194.0 11 5097.0 12 4931.0 13 5499.5 14 6087.0 15 6801.5 16 7721.0 17 8854.5 18 9922.0 19 11225.0 20 13352.5 21 16228.5 22 20440.5 23 27019.5 24 35230.5 25 45015.0 26 58277.5 27 75584.5 28 98242.5 29 121602.0 30 141399.0 31 161297.0 32 181029.5 33 199732.0 34 217755.5 35 234297.5 36 248941.5 37 262225.0 38 273397.5 39 281431.0 40 288475.5 41 291705.5 42 290499.5 43 291276.0 44 290459.0 45 286444.0 46 281403.0 47 275096.5 48 268741.0 49 264959.0 50 259187.0 51 247192.0 52 232799.0 53 222166.0 54 214449.5 55 202810.5 56 186262.0 57 167144.0 58 148964.0 59 132245.5 60 113150.5 61 92888.0 62 76604.0 63 61743.5 64 48180.0 65 37711.0 66 29547.5 67 22641.0 68 17127.0 69 12946.5 70 9279.5 71 6510.5 72 4791.5 73 3411.0 74 2298.0 75 1474.5 76 858.0 77 530.0 78 397.5 79 301.0 80 183.5 81 75.0 82 34.0 83 16.0 84 10.0 85 7.0 86 3.0 87 4.0 88 6.5 89 5.0 90 1.5 91 0.0 92 1.0 93 1.0 94 1.5 95 1.5 96 0.5 97 0.5 98 1.0 99 7.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060013997576029515 2 2.804392410094837E-4 3 2.438602095734641E-5 4 0.11688219844856135 5 0.002072811781374445 6 0.0018289515718009808 7 0.0 8 0.0 9 3.6579031436019616E-5 10-11 0.0 12-13 1.8289515718009808E-5 14-15 6.0965052393366025E-6 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.2802661002606866E-4 30-31 2.011846728981079E-4 32-33 1.8289515718009808E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.7070214670142489E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2193010478673205E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.0973709430805884E-4 80-81 2.987287567274935E-4 82-83 2.987287567274935E-4 84-85 0.0 86-87 6.0965052393366025E-6 88-89 6.706155763270263E-5 90-91 6.706155763270263E-5 92-93 6.462295553696799E-4 94-95 7.071946077630459E-4 96-97 6.0965052393366025E-6 98-99 3.6579031436019616E-5 100 9.388618068578369E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8201420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.04745648286595 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86376263704625 42.70787241180501 2 14.701344256621269 16.77348593438466 3 4.6365857649788715 7.9351627397012345 4 1.9868438074741022 4.533775425805221 5 1.045036457977769 2.9808335929747565 6 0.6238342782011306 2.135289530291945 7 0.42383241674992034 1.6924992945398287 8 0.28669007362440785 1.308395159932643 9 0.21239291400402247 1.0904827967022204 >10 1.1219813394294569 11.437070000643603 >50 0.06633064958163998 2.582502221683364 >100 0.029528706003736693 3.0179308620531966 >500 0.0011748089186705013 0.4419375135784247 >1k 5.764843210452643E-4 0.5592975980784554 >5k 2.1351266922200366E-5 0.08472654733481638 >10k+ 6.405380076660109E-5 0.7187383704906334 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23064 0.28121959368011884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22767 0.27759826956795286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13177 0.16066729907747682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.438602095734641E-5 0.0 3 0.0 0.0 0.0 2.438602095734641E-5 0.0 4 0.0 0.0 0.0 2.438602095734641E-5 0.0 5 0.0 1.2193010478673205E-5 0.0 2.438602095734641E-5 0.0 6 0.0 1.2193010478673205E-5 0.0 2.438602095734641E-5 0.0 7 0.0 1.2193010478673205E-5 0.0 2.438602095734641E-5 0.0 8 0.0 1.2193010478673205E-5 0.0 2.438602095734641E-5 0.0 9 0.0 1.2193010478673205E-5 0.0 6.096505239336603E-5 0.0 10-11 0.0 1.2193010478673205E-5 0.0 7.925456811137584E-5 0.0 12-13 0.0 1.2193010478673205E-5 0.0 1.2193010478673206E-4 0.0 14-15 0.0 1.2193010478673205E-5 0.0 1.3412311526540526E-4 0.0 16-17 0.0 1.2193010478673205E-5 0.0 1.4021962050474185E-4 0.0 18-19 0.0 1.2193010478673205E-5 0.0 1.950881676587713E-4 0.0 20-21 0.0 1.2193010478673205E-5 0.0 3.1092176720616673E-4 0.0 22-23 0.0 1.8289515718009808E-5 0.0 7.437736391990655E-4 0.0 24-25 0.0 2.438602095734641E-5 0.0 0.0016155738884241996 0.0 26-27 0.0 2.438602095734641E-5 0.0 0.0032433407873270724 0.0 28-29 0.0 3.6579031436019616E-5 0.0 0.00985195246676795 0.0 30-31 0.0 3.6579031436019616E-5 0.0 0.030872702532000557 0.0 32-33 0.0 3.6579031436019616E-5 0.0 0.0824613298672669 0.0 34-35 0.0 3.6579031436019616E-5 0.0 0.1722750450531737 0.0 36-37 0.0 3.6579031436019616E-5 0.0 0.31766450200087304 0.0 38-39 0.0 3.6579031436019616E-5 0.0 0.5197270716534454 0.0 40-41 0.0 3.6579031436019616E-5 0.0 0.734092388878999 0.0 42-43 0.0 3.6579031436019616E-5 0.0 0.9591083007576737 0.0 44-45 0.0 3.6579031436019616E-5 0.0 1.2356213923930248 0.0 46-47 0.0 3.6579031436019616E-5 0.0 1.5753064713183815 0.0 48-49 0.0 3.6579031436019616E-5 0.0 1.9353904567745586 0.0 50-51 0.0 3.6579031436019616E-5 0.0 2.296712032794321 0.0 52-53 0.0 3.6579031436019616E-5 0.0 2.657661721994484 0.0 54-55 0.0 3.6579031436019616E-5 0.0 3.0580740896088727 0.0 56-57 0.0 4.877204191469282E-5 0.0 3.532162723040644 0.0 58-59 0.0 6.096505239336603E-5 1.2193010478673205E-5 4.04397653089343 0.0 60-61 0.0 6.096505239336603E-5 1.2193010478673205E-5 4.566434836894098 0.0 62-63 0.0 6.096505239336603E-5 1.2193010478673205E-5 5.072883720136269 0.0 64-65 0.0 6.706155763270263E-5 1.2193010478673205E-5 5.617344313545702 0.0 66-67 0.0 7.315806287203923E-5 1.2193010478673205E-5 6.234316740271806 0.0 68-69 0.0 7.315806287203923E-5 1.2193010478673205E-5 6.8934952727698375 0.0 70-71 0.0 7.315806287203923E-5 1.2193010478673205E-5 7.575395480294876 0.0 72-73 0.0 7.315806287203923E-5 1.2193010478673205E-5 8.270232471937787 0.0 74-75 0.0 7.925456811137584E-5 1.2193010478673205E-5 8.954437158443294 0.0 76-77 0.0 8.535107335071244E-5 1.2193010478673205E-5 9.681981900695245 0.0 78-79 0.0 8.535107335071244E-5 1.2193010478673205E-5 10.471491522199814 0.0 80-81 0.0 8.535107335071244E-5 1.2193010478673205E-5 11.296245040492987 0.0 82-83 1.2193010478673205E-5 8.535107335071244E-5 1.2193010478673205E-5 12.114127797381428 0.0 84-85 1.2193010478673205E-5 8.535107335071244E-5 1.2193010478673205E-5 12.906880759673324 0.0 86-87 1.2193010478673205E-5 8.535107335071244E-5 1.2193010478673205E-5 13.72308088111571 0.0 88 1.2193010478673205E-5 8.535107335071244E-5 1.2193010478673205E-5 14.364792926103041 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14970 0.0 56.16755 1 GTATCAA 26245 0.0 39.428204 1 ATCAACG 32845 0.0 31.18739 3 TCAACGC 33140 0.0 30.98076 4 GTGGTAT 6065 0.0 30.583786 1 TATCAAC 33805 0.0 30.385237 2 CAACGCA 34200 0.0 29.971655 5 AACGCAG 36140 0.0 28.440107 6 TGGTATC 6730 0.0 25.868814 2 ACGCAGA 41945 0.0 24.39208 7 CGCAGAG 41680 0.0 24.36675 8 GTACATG 23315 0.0 23.504087 1 GCAGAGT 45155 0.0 22.054424 9 TACATGG 23795 0.0 22.028723 2 GAGTACT 31615 0.0 21.101475 12-13 ACATGGG 24255 0.0 21.028963 3 CAGAGTA 43935 0.0 20.190493 10-11 AGAGTAC 41610 0.0 19.782585 10-11 GTACTTT 34680 0.0 19.019712 14-15 AGTACTT 32695 0.0 18.78732 12-13 >>END_MODULE