FastQCFastQC Report
Wed 25 May 2016
SRR2049528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815510
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71050.042.8662341
GTATCAA109750.036.5657041
TCAACGC113900.033.790554
ATCAACG116500.032.9924963
CAACGCA122950.031.3865785
AACGCAG133050.029.1571226
TATCAAC135100.028.9048232
GTACATG201800.025.542931
TACATGG203550.024.3140052
ACGCAGA158850.024.0666987
GTGGTAT41150.024.0076641
CGCAGAG161500.023.1373658
ACATGGG212600.022.340143
GCAGAGT185800.019.2104579
TGGTATC46800.018.3723432
CATGGGG145300.018.069784
CAGAGTA175650.016.48307610-11
AGAGTAC160350.015.93991110-11
GAGTACA153550.015.9012391
CATGGGT48950.015.8924924
Produced by FastQC (version 0.11.5)