##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049528_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6815510 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.731501824514968 31.0 31.0 34.0 27.0 34.0 2 30.849172402358736 31.0 31.0 34.0 27.0 34.0 3 31.03435267500158 31.0 31.0 34.0 27.0 34.0 4 34.6055725837098 37.0 35.0 37.0 32.0 37.0 5 34.61263309715634 37.0 35.0 37.0 32.0 37.0 6 34.56635233460152 37.0 35.0 37.0 31.0 37.0 7 30.680600131171403 37.0 33.0 37.0 2.0 37.0 8 32.44941581774511 37.0 33.0 37.0 17.0 37.0 9 35.20753545956209 39.0 34.0 39.0 28.0 39.0 10-11 35.737060102618884 39.0 36.0 39.0 30.0 39.0 12-13 35.88853526735343 39.0 37.0 39.0 30.0 39.0 14-15 37.0697993253623 40.0 37.0 41.0 31.0 41.0 16-17 37.08346521390182 40.0 37.0 41.0 31.0 41.0 18-19 37.07825335154669 40.0 37.0 41.0 31.0 41.0 20-21 37.14961059407146 40.0 37.0 41.0 31.0 41.0 22-23 37.11133957693555 40.0 37.0 41.0 31.0 41.0 24-25 37.08668646953787 40.0 37.0 41.0 30.5 41.0 26-27 37.11120671820598 40.0 37.0 41.0 31.0 41.0 28-29 37.09861917890224 40.0 37.0 41.0 31.0 41.0 30-31 37.02406334962461 40.0 37.0 41.0 30.0 41.0 32-33 36.981530215640504 40.0 37.0 41.0 30.0 41.0 34-35 36.925575488848224 40.0 37.0 41.0 30.0 41.0 36-37 36.76242452875867 40.0 37.0 41.0 30.0 41.0 38-39 36.65374359365624 40.0 36.5 41.0 30.0 41.0 40-41 36.5797489109399 40.0 36.0 41.0 30.0 41.0 42-43 36.46711669412854 40.0 36.0 41.0 30.0 41.0 44-45 36.350863251612864 40.0 36.0 41.0 30.0 41.0 46-47 36.197430639820055 40.0 35.5 41.0 29.0 41.0 48-49 36.11645973668882 39.5 35.0 41.0 29.0 41.0 50-51 35.977488038312615 39.0 35.0 41.0 29.0 41.0 52-53 35.85470118890589 39.0 35.0 41.0 28.0 41.0 54-55 35.730323042589625 39.0 35.0 41.0 28.0 41.0 56-57 35.524763003795755 39.0 35.0 41.0 28.0 41.0 58-59 35.33532523611586 39.0 35.0 40.5 28.0 41.0 60-61 35.10470331640626 38.0 34.0 40.0 27.5 41.0 62-63 34.85706528198183 38.0 34.0 40.0 27.0 41.0 64-65 34.49098086570191 37.5 34.0 40.0 26.0 41.0 66-67 31.016923458405902 36.0 30.0 40.0 6.5 41.0 68-69 31.36094855704122 36.0 30.0 39.0 11.0 41.0 70-71 31.513895438492497 35.0 30.0 39.0 15.5 41.0 72-73 32.732196636788736 35.0 31.5 39.0 23.5 40.5 74-75 32.809793397706116 35.0 33.0 38.5 24.0 40.0 76-77 32.513110904393066 35.0 33.0 37.0 23.5 39.5 78-79 29.171931961071145 35.0 29.0 36.5 6.5 39.0 80-81 30.938653160218387 35.0 29.5 36.0 18.5 39.0 82-83 31.343027741137494 35.0 31.5 36.0 20.0 37.5 84-85 29.525495964351897 34.5 30.0 35.5 11.0 37.0 86-87 29.159972694633268 34.0 28.0 35.0 10.5 37.0 88-89 30.38592592483908 34.0 30.5 35.0 18.0 36.0 90-91 30.53160218384244 35.0 31.5 35.0 16.0 36.0 92-93 30.3791215917811 35.0 32.0 35.0 9.0 36.0 94-95 30.171938416934317 35.0 31.5 35.0 2.0 36.0 96-97 29.974969151244736 35.0 31.0 35.0 2.0 35.0 98-99 29.775275951469517 34.5 31.0 35.0 2.0 35.0 100 29.552742201243927 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41390267112459256 1101 2 0.42902521882533406 1101 3 0.4487130225360012 1101 4 0.48042269068517385 1101 5 0.4779984861182598 1101 6 0.4377977808835922 1101 7 -11.992527470247705 1101 8 -6.411011142091933 1101 9 -2.07317559827969 1101 10-11 -0.027557365912336707 1101 12-13 0.4758735973729529 1101 14-15 0.707664632566015 1101 16-17 0.777229896332301 1101 18-19 0.7238631625620542 1101 20-21 0.7367896447168221 1101 22-23 0.7116411691498854 1101 24-25 0.732690799387143 1101 26-27 0.6627307363148134 1101 28-29 0.6093993287992561 1101 30-31 0.7103071040747295 1101 32-33 0.7304291479236724 1101 34-35 0.7468587638453883 1101 36-37 0.7611111399477721 1101 38-39 0.7647307613088259 1101 40-41 0.7514923992794778 1101 42-43 0.717646709230614 1101 44-45 0.6737318231096623 1101 46-47 0.6336163652399875 1101 48-49 0.5315308181807197 1101 50-51 0.682424600397951 1101 52-53 0.6291932014147505 1101 54-55 0.5891343275548877 1101 56-57 0.5771852248363416 1101 58-59 0.5560497115665157 1101 60-61 0.5584059963581538 1101 62-63 0.5988152048650619 1101 64-65 0.4630630172982606 1101 66-67 -10.108077575369357 1101 68-69 -8.019648995753379 1101 70-71 -6.531242376614124 1101 72-73 -1.4061497004121364 1101 74-75 -0.10405009678623856 1101 76-77 0.18838254353148187 1101 78-79 3.315243492603617 1101 80-81 1.1984603399335896 1101 82-83 0.527744919108855 1101 84-85 -5.220841897460378 1101 86-87 -5.762752823432706 1101 88-89 -1.1789785379329842 1101 90-91 0.12783924158638627 1101 92-93 0.4826631825485528 1101 94-95 0.5046595135712941 1101 96-97 0.6339051118573344 1101 98-99 0.6513227979582403 1101 100 0.7879429377627076 1102 1 0.3040164742982938 1102 2 0.3120318248309957 1102 3 0.33845489304468046 1102 4 0.3554761814262761 1102 5 0.3241491046175611 1102 6 0.32452876220179405 1102 7 -12.214421872295258 1102 8 -6.589332458591159 1102 9 -2.218772793971297 1102 10-11 -0.18180259307269608 1102 12-13 0.38912882764538637 1102 14-15 0.5971617519933758 1102 16-17 0.5965903987385417 1102 18-19 0.5973542068020734 1102 20-21 0.5479102449384357 1102 22-23 0.5633192332899313 1102 24-25 0.6019778999130949 1102 26-27 0.5878449254985512 1102 28-29 0.5446361677652405 1102 30-31 0.5331662313474794 1102 32-33 0.5363141108263605 1102 34-35 0.5378216582489586 1102 36-37 0.7052158071144419 1102 38-39 0.68165610740391 1102 40-41 0.5847876949947874 1102 42-43 0.5680708295802575 1102 44-45 0.5623466533535719 1102 46-47 0.6173089623307817 1102 48-49 0.5603461130324163 1102 50-51 0.6274167417133825 1102 52-53 0.5814999548675246 1102 54-55 0.5696351614019974 1102 56-57 0.600631081912745 1102 58-59 0.5351104444665395 1102 60-61 0.447902810466843 1102 62-63 0.47601522218099035 1102 64-65 0.3121847790393488 1102 66-67 -10.238137828919065 1102 68-69 -6.6222570169547055 1102 70-71 -6.149984107112385 1102 72-73 -0.9689015964956198 1102 74-75 -0.07530831285721717 1102 76-77 0.12502121502014774 1102 78-79 3.2142792580691513 1102 80-81 1.1506573531318622 1102 82-83 0.49914450862970483 1102 84-85 -5.436744562303566 1102 86-87 -5.9550644140056015 1102 88-89 -1.3716310277086272 1102 90-91 -0.048054322579496045 1102 92-93 0.3094567225724525 1102 94-95 0.3071354571859146 1102 96-97 0.40108150623135685 1102 98-99 0.36890626935740656 1102 100 0.5029423391200218 1103 1 0.21050319660433914 1103 2 0.2259731192361336 1103 3 0.2467382866734269 1103 4 0.28057468873057445 1103 5 0.26496648047645976 1103 6 0.2805626477880381 1103 7 -12.063726688867646 1103 8 -6.528966125538986 1103 9 -2.2628295515281565 1103 10-11 -0.26772151241272724 1103 12-13 0.29688584885693814 1103 14-15 0.49319873060580477 1103 16-17 0.5052618102912518 1103 18-19 0.5571190736461347 1103 20-21 0.4613894172286237 1103 22-23 0.49900128030476765 1103 24-25 0.5977320319139352 1103 26-27 0.5341422320601907 1103 28-29 0.5190006866912427 1103 30-31 0.5176107836419419 1103 32-33 0.5670680975455085 1103 34-35 0.567194416120806 1103 36-37 0.6100402120806336 1103 38-39 0.6721379351907757 1103 40-41 0.6305234246478761 1103 42-43 0.595678056944422 1103 44-45 0.6069546391302865 1103 46-47 0.6159796243141855 1103 48-49 0.5709659415831752 1103 50-51 0.5725297182320759 1103 52-53 0.49775387063024823 1103 54-55 0.5141749490043708 1103 56-57 0.5854773591430558 1103 58-59 0.5902797565935174 1103 60-61 0.5435150822640509 1103 62-63 0.4219225421464472 1103 64-65 0.341562000638568 1103 66-67 -10.226237373829427 1103 68-69 -8.131024051233187 1103 70-71 -6.647260233287639 1103 72-73 -1.454408421155776 1103 74-75 -0.29466259258215644 1103 76-77 0.015227491863043952 1103 78-79 3.205956017251477 1103 80-81 1.1251278750552913 1103 82-83 0.41446778987358357 1103 84-85 -5.3759467005132 1103 86-87 -5.870771081003536 1103 88-89 -1.2801406291440962 1103 90-91 0.04906360446551261 1103 92-93 0.4113130962487581 1103 94-95 0.5213696415892315 1103 96-97 0.597617180045745 1103 98-99 0.6403927474217674 1103 100 0.7317921460638139 1104 1 0.0945372300226559 1104 2 0.09081561170454933 1104 3 0.09710869542680811 1104 4 0.17006894069851342 1104 5 0.16216631380290636 1104 6 0.16987240931894831 1104 7 -12.1604696570035 1104 8 -6.673702134534203 1104 9 -2.4299532131622215 1104 10-11 -0.42798783127220474 1104 12-13 0.16797735031389038 1104 14-15 0.3273354659751746 1104 16-17 0.3438504385800627 1104 18-19 0.3802905186445571 1104 20-21 0.3860815717652599 1104 22-23 0.32922302243754586 1104 24-25 0.4582966511193973 1104 26-27 0.38854578278565555 1104 28-29 0.35415118125825984 1104 30-31 0.3193643549106042 1104 32-33 0.3377739130441171 1104 34-35 0.340749151892787 1104 36-37 0.37918849521077647 1104 38-39 0.4522776379803588 1104 40-41 0.4667951606988794 1104 42-43 0.37885644848041267 1104 44-45 0.37075219285492267 1104 46-47 0.39844399591553525 1104 48-49 0.3605123889120634 1104 50-51 0.40331761000006594 1104 52-53 0.4600825308814933 1104 54-55 0.40214128480774747 1104 56-57 0.41767900195283403 1104 58-59 0.3581465105076589 1104 60-61 0.3459937713909582 1104 62-63 0.33894058285478934 1104 64-65 0.24008812614412278 1104 66-67 -10.262461458045529 1104 68-69 -8.233524814792403 1104 70-71 -6.711586954272054 1104 72-73 -1.6381826156577404 1104 74-75 -0.3742541663160921 1104 76-77 -0.08070249316318723 1104 78-79 3.0391672951661413 1104 80-81 0.9607773998985927 1104 82-83 0.3191217452769095 1104 84-85 -5.453069919434434 1104 86-87 -5.9559281329784035 1104 88-89 -1.4143857940366615 1104 90-91 -0.1403462982659036 1104 92-93 0.17894711988163792 1104 94-95 0.2173099147719455 1104 96-97 0.32516395834561607 1104 98-99 0.362639936069872 1104 100 0.4367877328831824 1105 1 0.13543378804555317 1105 2 0.15501665575562384 1105 3 0.17975900764824715 1105 4 0.20050100266298188 1105 5 0.1807422964140386 1105 6 0.1963781150128412 1105 7 -12.231572745427368 1105 8 -6.692447670091827 1105 9 -2.413730888831658 1105 10-11 -0.40254222366377235 1105 12-13 0.14226358566677533 1105 14-15 0.3056254361020976 1105 16-17 0.42645508479947125 1105 18-19 0.38526429768323567 1105 20-21 0.3935186375073414 1105 22-23 0.35576243053179724 1105 24-25 0.43449603632099354 1105 26-27 0.38321195307824496 1105 28-29 0.3649885049764521 1105 30-31 0.3915044276059234 1105 32-33 0.400264515492168 1105 34-35 0.45683453050655487 1105 36-37 0.5169964870245138 1105 38-39 0.4276324296996137 1105 40-41 0.4724559535582813 1105 42-43 0.39518354026412084 1105 44-45 0.3344230871579228 1105 46-47 0.30845698922939846 1105 48-49 0.30021402569156663 1105 50-51 0.32507741736468176 1105 52-53 0.30585160774732856 1105 54-55 0.2660215745788648 1105 56-57 0.25003527876612 1105 58-59 0.19403288257193196 1105 60-61 0.1843530891571561 1105 62-63 0.11503292214474214 1105 64-65 0.023909867181558297 1105 66-67 -10.49495328619387 1105 68-69 -8.52274029553611 1105 70-71 -6.895169404877823 1105 72-73 -1.8098169141408036 1105 74-75 -0.586276044436751 1105 76-77 -0.28810226209864 1105 78-79 2.8303927432991323 1105 80-81 0.7843387107088411 1105 82-83 0.08485218208171119 1105 84-85 -5.60920156402171 1105 86-87 -6.12303475774803 1105 88-89 -1.6018316213376806 1105 90-91 -0.3059195824384986 1105 92-93 0.0029398272263954084 1105 94-95 0.03310215657911897 1105 96-97 0.15961620394705633 1105 98-99 0.18948282263528782 1105 100 0.3215951652787119 1106 1 0.1690727576581068 1106 2 0.19167638247593644 1106 3 0.20272229084464044 1106 4 0.18934392452707272 1106 5 0.18869033941795266 1106 6 0.17932194851454142 1106 7 -12.23731725795243 1106 8 -6.674797247866792 1106 9 -2.5152410253204707 1106 10-11 -0.3551153398386333 1106 12-13 0.25209911563001697 1106 14-15 0.35532478754715413 1106 16-17 0.35377784213856955 1106 18-19 0.38493996787449447 1106 20-21 0.3615267244246638 1106 22-23 0.39955534287403793 1106 24-25 0.453267042241734 1106 26-27 0.4160681739785659 1106 28-29 0.3739864701718716 1106 30-31 0.3514044048237679 1106 32-33 0.3847669770041975 1106 34-35 0.4483074472945674 1106 36-37 0.5122343427788536 1106 38-39 0.45517281288245215 1106 40-41 0.44675717619062283 1106 42-43 0.43915367414498263 1106 44-45 0.4287159367688602 1106 46-47 0.42122215141897357 1106 48-49 0.4081472606686063 1106 50-51 0.41889353898056214 1106 52-53 0.4052426327820484 1106 54-55 0.37319005216641443 1106 56-57 0.4210454230309182 1106 58-59 0.4008502218779242 1106 60-61 0.39644545625296246 1106 62-63 0.2600866953578489 1106 64-65 0.1371233404223986 1106 66-67 -10.340137369856642 1106 68-69 -8.290414536403226 1106 70-71 -6.835204737405693 1106 72-73 -1.6787516467061927 1106 74-75 -0.44520004052841955 1106 76-77 -0.15036128398528348 1106 78-79 2.9791149518926012 1106 80-81 0.8528171384679872 1106 82-83 0.20748140239741275 1106 84-85 -5.5688119416933155 1106 86-87 -6.056839115749224 1106 88-89 -1.465238265307928 1106 90-91 -0.24847227019071028 1106 92-93 0.11486694425646249 1106 94-95 0.19823920410155793 1106 96-97 0.31403513357008705 1106 98-99 0.38132752146260174 1106 100 0.4733693844777349 1107 1 0.09367075618173004 1107 2 0.13615333933821816 1107 3 0.09602923731783264 1107 4 0.07619534947953355 1107 5 0.11711127574195501 1107 6 0.08122915123343688 1107 7 -12.17281950375018 1107 8 -6.663840547988642 1107 9 -2.5620084704174104 1107 10-11 -0.5416223893076975 1107 12-13 0.04595399021360436 1107 14-15 0.1515821667796473 1107 16-17 0.21980937123431232 1107 18-19 0.2457956038254281 1107 20-21 0.2226910731509122 1107 22-23 0.2923165363655045 1107 24-25 0.2899494047308906 1107 26-27 0.19571497430734297 1107 28-29 0.20502494783637815 1107 30-31 0.24988123707269239 1107 32-33 0.2574799164504782 1107 34-35 0.1989838436028677 1107 36-37 0.29835044225134055 1107 38-39 0.3474912420280347 1107 40-41 0.294106628221833 1107 42-43 0.28876279647187886 1107 44-45 0.23089397374433673 1107 46-47 0.2581328843373498 1107 48-49 0.2176515215431536 1107 50-51 0.2332598814192579 1107 52-53 0.222056248103911 1107 54-55 0.20533823871029 1107 56-57 0.21681394525953124 1107 58-59 0.20341981365508843 1107 60-61 0.1449867142580672 1107 62-63 0.1252863823852266 1107 64-65 -0.03436804369044211 1107 66-67 -10.251584292748404 1107 68-69 -8.27668015382254 1107 70-71 -6.827044162717531 1107 72-73 -1.8834980857375996 1107 74-75 -0.561248967941367 1107 76-77 -0.33209706758026414 1107 78-79 2.8011822623431115 1107 80-81 0.7091427299524611 1107 82-83 0.06503977472349476 1107 84-85 -5.5672759401406715 1107 86-87 -6.055891248737712 1107 88-89 -1.6317581243501884 1107 90-91 -0.3985649536346543 1107 92-93 -0.025401398998656788 1107 94-95 0.09684597904334424 1107 96-97 0.13714168722914621 1107 98-99 0.15675032769210517 1107 100 0.21885515625235286 1108 1 -0.021060324659103458 1108 2 -0.035708811902178894 1108 3 0.01854922388528024 1108 4 -0.008378660786775072 1108 5 -0.02382735921711543 1108 6 0.049006684440911386 1108 7 -12.259497430057383 1108 8 -6.77685049470994 1108 9 -2.746018969538369 1108 10-11 -0.6117189869349886 1108 12-13 -0.042289703400008705 1108 14-15 0.1316752228408049 1108 16-17 0.08692808142696151 1108 18-19 0.14613201733361336 1108 20-21 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2216 20-21 -1.5889390104506091 2216 22-23 -1.4107697417834615 2216 24-25 -1.39880240332824 2216 26-27 -1.8245139611220225 2216 28-29 -2.032259479064777 2216 30-31 -1.913398748790101 2216 32-33 -2.825278860323621 2216 34-35 -1.8603469021837498 2216 36-37 -1.575100670782021 2216 38-39 -1.765963230555876 2216 40-41 -1.7873487975272653 2216 42-43 -1.4140792576559633 2216 44-45 -1.578068826807602 2216 46-47 -1.7278427758275186 2216 48-49 -1.900725160487589 2216 50-51 -2.429191513541298 2216 52-53 -2.127512185625335 2216 54-55 -1.7241580670507943 2216 56-57 -1.7479156727045577 2216 58-59 -2.146841094375887 2216 60-61 -2.28660121934071 2216 62-63 -2.117476469485432 2216 64-65 -1.8039598252102635 2216 66-67 1.5731401793851916 2216 68-69 0.7728051778203664 2216 70-71 0.4241181471580404 2216 72-73 -1.281378877840826 2216 74-75 -2.133064737799856 2216 76-77 -1.7820711600582797 2216 78-79 0.7378463460127769 2216 80-81 -0.7119926998996533 2216 82-83 -1.5541894746878704 2216 84-85 0.2020530824415765 2216 86-87 0.24116301017506814 2216 88-89 -1.0241820121505043 2216 90-91 -1.3720661238677536 2216 92-93 -1.4267065104529628 2216 94-95 -1.399763940792237 2216 96-97 -1.3605445373676162 2216 98-99 -1.4076084213287352 2216 100 -2.5890508309289295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105927.0 3 20850.0 4 12123.0 5 10895.0 6 10257.0 7 11065.0 8 12294.0 9 13560.0 10 15138.0 11 17011.0 12 18916.0 13 21052.0 14 24009.0 15 26415.0 16 28102.0 17 28942.0 18 27453.0 19 26172.0 20 25446.0 21 26100.0 22 27929.0 23 32441.0 24 38677.0 25 48458.0 26 60615.0 27 73187.0 28 91569.0 29 115698.0 30 142948.0 31 174219.0 32 231416.0 33 325349.0 34 488330.0 35 666702.0 36 741880.0 37 1237874.0 38 1380559.0 39 454885.0 40 1047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22818814796161 20.304790517054396 14.41287630311403 23.05414503186996 2 15.39328923975031 20.426454799920123 40.46670803405194 23.713547926277627 3 18.331018727399577 25.34504501926882 30.31350255266921 26.010433700662393 4 12.83968630311873 17.18350099605759 38.34561268802907 31.631200012794615 5 13.147896837431933 37.806496161108576 35.201065120516404 13.844541880943083 6 30.628244365086793 37.635603419258906 17.96691198803629 13.769240227618006 7 27.105720324845905 32.648055827868724 22.442558890188394 17.80366495709698 8 24.60748701453905 36.69368984581778 20.55582952229379 18.142993617349376 9 24.657156984583693 17.701521969742544 21.77040309529294 35.87091795038082 10-11 23.59971594202048 27.4329433894162 28.813449030226646 20.153891638336678 12-13 24.042258026178526 24.97389043519854 28.838069344773903 22.14578219384903 14-15 21.585537986152172 27.310502075413286 25.97449787323326 25.129462065201285 16-17 21.165548845842846 30.50933366082248 26.746986608305516 21.578130885029154 18-19 21.14737561825894 29.537452076220266 28.47893261105919 20.836239694461604 20-21 22.201229255037408 28.745347009981643 28.059793030895708 20.99363070408524 22-23 21.9876053259713 28.584303620507818 28.049295811845575 21.37879524167531 24-25 21.753185014767787 28.68496268070915 28.048289856518444 21.513562448004624 26-27 21.458023729588714 28.70381693960949 28.096554038335707 21.74160529246609 28-29 21.48087230449372 28.739734810747837 28.249463356373916 21.52992952838452 30-31 21.790234464496013 29.008066088089237 27.856435384617978 21.345264062796772 32-33 21.45655277448056 28.840651690042275 28.19473524358412 21.50806029189305 34-35 21.62068794001679 28.588049282320448 28.171491864068223 21.619770913594543 36-37 21.897423809621277 28.73590008201148 27.847067046124724 21.519609062242523 38-39 21.68099159754366 28.878093735359673 27.97462463174053 21.466290035356135 40-41 21.60282367737878 28.637947023623106 28.130075968641815 21.6291533303563 42-43 21.56623282510258 28.676584785004604 28.09519732686045 21.661985063032365 44-45 21.645579928105054 28.815024576333357 28.004225662093756 21.53516983346783 46-47 21.616386698788762 28.870202273377366 27.890333652729982 21.62307737510389 48-49 21.561047812745514 28.697940062418258 27.98457069783926 21.756441426996965 50-51 21.56888047896186 28.664943484258398 27.95941832799855 21.806757708781195 52-53 21.52654615844935 28.706279872640238 28.017283847185602 21.749890121724807 54-55 21.619234769732657 28.910477616359554 27.85658436053983 21.61370325336795 56-57 21.535227131586797 28.76724498991001 27.846714983071845 21.85081289543134 58-59 21.42380759717658 28.485769737109973 28.112421809575753 21.97800085613769 60-61 21.66226880796216 28.72383256952818 27.84643481460766 21.767463807902 62-63 21.484922619991302 28.788448912848825 27.868401933662035 21.858226533497835 64-65 21.502109178410816 28.5920592183935 27.960362118422115 21.945469484773568 66-67 21.41453382724803 28.557818206287163 27.91985111531118 22.10779685115363 68-69 21.81317115820269 28.682732097869955 27.726354451917146 21.777742292010206 70-71 21.537967065299583 28.497852893353954 27.874391198260334 22.08978884308613 72-73 21.36798104538549 28.556814690413145 28.079780052287173 21.995424211914187 74-75 21.414843960463216 28.573124329819095 27.952442172961987 22.0595895367557 76-77 21.46228690487379 28.568149098486096 27.778390386258085 22.191173610382034 78-79 21.560188789921693 28.553905590923268 27.878150101995047 22.007755517159993 80-81 21.33784513674732 28.46896793780807 27.97480499727006 22.21838192817455 82-83 21.294920083422344 28.473718418115507 28.100303138550366 22.131058359911787 84-85 21.403045115674708 28.344781437218085 27.953939463419392 22.298233983687815 86-87 21.467653822180537 28.42569599569907 27.913632794112985 22.193017388007405 88-89 21.277499829063743 28.3116589092418 28.038447012547746 22.372394249146712 90-91 21.250040442191438 28.240571357009582 27.977420161611764 22.531968039187216 92-93 21.337015853908426 28.292459003211835 27.904190588780953 22.466334554098783 94-95 21.286872515206806 28.076411494591873 28.075090918028145 22.56162507217318 96-97 21.24820285150618 28.090407619726204 28.077921027956098 22.583468500811517 98-99 21.26314243379958 28.0968998720202 27.98641788324756 22.653539810932664 100 21.381781621448397 27.97726939578106 27.917505303274524 22.723443679496018 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3838.0 1 3029.5 2 2588.5 3 2904.0 4 2670.5 5 2360.5 6 2169.5 7 2074.0 8 2084.5 9 2074.0 10 1933.0 11 1805.5 12 1697.5 13 1702.0 14 1887.0 15 2124.5 16 2456.0 17 2975.5 18 3871.0 19 5145.0 20 7158.0 21 10147.5 22 14645.5 23 21373.5 24 29778.5 25 40102.0 26 53607.5 27 69871.5 28 88822.0 29 108336.5 30 126150.0 31 143459.5 32 159736.0 33 174135.5 34 187935.5 35 201373.0 36 212539.0 37 222523.5 38 230801.0 39 236305.0 40 241432.5 41 243544.0 42 241664.0 43 240604.5 44 238375.5 45 233387.5 46 229313.0 47 224366.0 48 218445.0 49 215011.5 50 210226.5 51 200793.5 52 189762.0 53 180601.5 54 172236.5 55 161675.0 56 148067.5 57 132197.5 58 118101.5 59 106870.0 60 93631.5 61 78803.5 62 66218.0 63 54968.0 64 44347.0 65 35148.5 66 27569.5 67 21188.5 68 15939.0 69 11948.5 70 8712.5 71 6343.0 72 4826.5 73 3467.0 74 2381.0 75 1587.0 76 1033.5 77 695.5 78 488.5 79 329.0 80 210.5 81 133.5 82 105.0 83 79.0 84 58.5 85 55.5 86 48.0 87 35.0 88 27.0 89 22.5 90 15.0 91 14.0 92 13.0 93 10.0 94 9.0 95 11.0 96 9.5 97 12.5 98 13.0 99 15.5 100 26.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.018428554869701606 2 0.0023035693587127007 3 0.001393879548265647 4 0.002068810697952171 5 0.0019220865349768397 6 0.0034040005810276855 7 11.605763912018324 8 4.4017248892599383E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4943107705806315E-4 18-19 0.0 20-21 0.0 22-23 2.9344832595066254E-5 24-25 0.0 26-27 1.5406037112409781E-4 28-29 0.0 30-31 7.336208148766564E-6 32-33 0.0 34-35 4.4017248892599383E-5 36-37 7.336208148766564E-6 38-39 4.4017248892599383E-5 40-41 7.336208148766564E-6 42-43 5.135345704136594E-5 44-45 1.4672416297533127E-4 46-47 8.143191045130885E-4 48-49 5.355431948599592E-4 50-51 2.8611211780189594E-4 52-53 1.980776200166972E-4 54-55 2.787759096531294E-4 56-57 0.0012985088423316817 58-59 2.5676728520682975E-4 60-61 0.0010564139734223852 62-63 0.0022228710690762685 64-65 8.803449778519876E-4 66-67 5.803058025004733 68-69 5.45244596515888 70-71 0.002575009060217064 72-73 0.0010564139734223852 74-75 0.0027290694313411618 76-77 0.005729578564186686 78-79 5.412265553128086 80-81 0.005120673287839062 82-83 0.004541112844086503 84-85 5.803292783665492 86-87 0.0034920350788128843 88-89 0.001525931294943445 90-91 0.0033233022913912533 92-93 0.0036754402825320484 94-95 0.0044750869707476035 96-97 0.0029271470513578587 98-99 0.0054581388626823235 100 0.0055901906093601215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6815510.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.53595413080703 #Duplication Level Percentage of deduplicated Percentage of total 1 91.6817204819421 80.25446878740755 2 5.917654275750907 10.36015026488211 3 1.2582386309853533 3.3042335726262992 4 0.4881357791435454 1.7091772469086053 5 0.23545233312178496 1.0305272316070033 6 0.1297762899957046 0.6816054820998186 7 0.08589496700096269 0.5263228524024418 8 0.05593681086602007 0.3917185688153249 9 0.03505682856497874 0.276185964351199 >10 0.11129736111891944 1.4067958323102487 >50 7.759788349299408E-4 0.044428896183492314 >100 4.275331130187066E-5 0.004131545161768705 >500 1.7509363505597887E-5 0.010253755244186952 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.9344832595066254E-5 0.0 5 0.0 1.4672416297533127E-5 0.0 4.401724889259938E-5 0.0 6 0.0 1.4672416297533127E-5 0.0 4.401724889259938E-5 0.0 7 0.0 1.4672416297533127E-5 0.0 5.868966519013251E-5 0.0 8 0.0 1.4672416297533127E-5 0.0 5.868966519013251E-5 0.0 9 2.9344832595066254E-5 1.4672416297533127E-5 0.0 5.868966519013251E-5 0.0 10-11 2.9344832595066254E-5 1.4672416297533127E-5 0.0 7.336208148766563E-5 0.0 12-13 4.401724889259938E-5 1.4672416297533127E-5 0.0 9.537070593396531E-5 0.0 14-15 4.401724889259938E-5 1.4672416297533127E-5 0.0 1.1737933038026502E-4 0.0 16-17 4.401724889259938E-5 1.4672416297533127E-5 0.0 1.3205174667779815E-4 0.0 18-19 4.401724889259938E-5 1.4672416297533127E-5 0.0 1.4672416297533127E-4 0.0 20-21 4.401724889259938E-5 1.4672416297533127E-5 0.0 1.9807762001669723E-4 0.0 22-23 4.401724889259938E-5 1.4672416297533127E-5 0.0 3.5213799114079507E-4 0.0 24-25 4.401724889259938E-5 1.4672416297533127E-5 0.0 6.529225252402242E-4 0.0 26-27 4.401724889259938E-5 2.9344832595066254E-5 0.0 0.0010123967245297856 0.0 28-29 4.401724889259938E-5 2.9344832595066254E-5 0.0 0.002714397015043628 0.0 30-31 4.401724889259938E-5 2.9344832595066254E-5 0.0 0.007717690972502425 0.0 32-33 5.135345704136594E-5 2.9344832595066254E-5 0.0 0.019257546390512227 0.0 34-35 5.868966519013251E-5 2.9344832595066254E-5 0.0 0.03877186006623129 0.0 36-37 5.868966519013251E-5 3.668104074383282E-5 0.0 0.06977467570291879 0.0 38-39 5.868966519013251E-5 4.401724889259938E-5 0.0 0.11171577768941723 0.0 40-41 5.868966519013251E-5 4.401724889259938E-5 0.0 0.15638594910725684 0.0 42-43 5.868966519013251E-5 4.401724889259938E-5 0.0 0.20284615531339548 0.0 44-45 5.868966519013251E-5 5.868966519013251E-5 0.0 0.26276096726437204 0.0 46-47 5.868966519013251E-5 5.868966519013251E-5 0.0 0.3367319540283853 0.0 48-49 9.537070593396531E-5 5.868966519013251E-5 0.0 0.41630780381805615 0.0 50-51 1.0270691408273189E-4 5.868966519013251E-5 0.0 0.49806250742791075 0.0 52-53 1.0270691408273189E-4 5.868966519013251E-5 0.0 0.5814531854549403 0.0 54-55 1.0270691408273189E-4 5.868966519013251E-5 0.0 0.6761636326555166 0.0 56-57 1.1004312223149846E-4 6.602587333889908E-5 0.0 0.7814382195903168 0.0 58-59 1.1737933038026502E-4 7.336208148766563E-5 0.0 0.8986268085587139 0.0 60-61 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.022095191702455 0.0 62-63 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.1506035498443992 0.0 64-65 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.2942391691890995 0.0 66-67 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.4597880422741658 0.0 68-69 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.6275964674690522 0.0 70-71 1.1737933038026502E-4 7.336208148766563E-5 0.0 1.8290414070260332 0.0 72-73 1.1737933038026502E-4 1.0270691408273189E-4 0.0 2.0499493068016923 0.0 74-75 1.4672416297533127E-4 1.0270691408273189E-4 0.0 2.255707936750148 0.0 76-77 1.4672416297533127E-4 1.0270691408273189E-4 0.0 2.472867034161787 0.0 78-79 1.4672416297533127E-4 1.0270691408273189E-4 0.0 2.7309841816679894 0.0 80-81 1.4672416297533127E-4 1.0270691408273189E-4 0.0 3.045524106046356 0.0 82-83 1.4672416297533127E-4 1.0270691408273189E-4 0.0 3.3726823084406012 0.0 84-85 1.613965792728644E-4 1.0270691408273189E-4 0.0 3.705181270367148 0.0 86-87 1.613965792728644E-4 1.0270691408273189E-4 0.0 4.101307165567947 0.0 88 1.613965792728644E-4 1.0270691408273189E-4 0.0 4.431407187429848 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7105 0.0 42.866234 1 GTATCAA 10975 0.0 36.565704 1 TCAACGC 11390 0.0 33.79055 4 ATCAACG 11650 0.0 32.992496 3 CAACGCA 12295 0.0 31.386578 5 AACGCAG 13305 0.0 29.157122 6 TATCAAC 13510 0.0 28.904823 2 GTACATG 20180 0.0 25.54293 1 TACATGG 20355 0.0 24.314005 2 ACGCAGA 15885 0.0 24.066698 7 GTGGTAT 4115 0.0 24.007664 1 CGCAGAG 16150 0.0 23.137365 8 ACATGGG 21260 0.0 22.34014 3 GCAGAGT 18580 0.0 19.210457 9 TGGTATC 4680 0.0 18.372343 2 CATGGGG 14530 0.0 18.06978 4 CAGAGTA 17565 0.0 16.483076 10-11 AGAGTAC 16035 0.0 15.939911 10-11 GAGTACA 15355 0.0 15.901239 1 CATGGGT 4895 0.0 15.892492 4 >>END_MODULE