FastQCFastQC Report
Wed 25 May 2016
SRR2049528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815510
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263290.3863100486977497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255960.3755551675516579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160340.23525752291464613No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.10499581102514706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139100.057.095051
GTATCAA250200.040.924411
GTGGTAT53650.032.4670261
ATCAACG313400.032.277833
TCAACGC317550.031.9004464
TATCAAC322900.031.488472
CAACGCA328800.030.8181655
AACGCAG343300.029.6527446
TGGTATC61800.027.1796932
ACGCAGA394400.025.6316997
CGCAGAG397650.025.2803848
GCAGAGT424450.023.3298459
GTACATG208650.022.6530061
TACATGG211150.021.4139672
CAGAGTA414850.020.8336110-11
GAGTACT307850.020.83091212-13
AGAGTAC401200.020.34172210-11
ACATGGG216500.020.0807043
AGTACTT317250.019.51003512-13
GTACTTT333300.019.03585214-15
Produced by FastQC (version 0.11.5)