##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049528_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6815510 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.832938254070495 33.0 31.0 34.0 30.0 34.0 2 31.916939011167177 33.0 31.0 34.0 30.0 34.0 3 31.93905474425245 33.0 31.0 34.0 30.0 34.0 4 35.59930364712252 37.0 35.0 37.0 33.0 37.0 5 35.72209915325485 37.0 35.0 37.0 33.0 37.0 6 35.79473832479154 37.0 35.0 37.0 35.0 37.0 7 35.78190685656686 37.0 35.0 37.0 33.0 37.0 8 35.762092785426184 37.0 35.0 37.0 33.0 37.0 9 37.476530296338794 39.0 37.0 39.0 34.0 39.0 10-11 37.44999853275837 39.0 37.0 39.0 34.5 39.0 12-13 37.4382358033368 39.0 37.0 39.0 34.0 39.0 14-15 38.699841611266066 40.0 38.0 41.0 34.0 41.0 16-17 38.70411311846069 40.0 38.0 41.0 34.0 41.0 18-19 38.78589768043771 40.0 38.0 41.0 35.0 41.0 20-21 38.819772034667984 40.0 38.0 41.0 35.0 41.0 22-23 38.793543036397864 40.0 38.0 41.0 35.0 41.0 24-25 38.75644317153082 40.0 38.0 41.0 34.0 41.0 26-27 38.66312631043018 40.0 38.0 41.0 34.0 41.0 28-29 38.57574693603267 40.0 38.0 41.0 34.0 41.0 30-31 38.45546415455337 40.0 38.0 41.0 34.0 41.0 32-33 38.30110314561934 40.0 38.0 41.0 34.0 41.0 34-35 38.203523727498016 40.0 38.0 41.0 34.0 41.0 36-37 38.06900848212386 40.0 38.0 41.0 33.5 41.0 38-39 37.98631334999142 40.0 37.0 41.0 33.0 41.0 40-41 37.866288582952706 40.0 37.0 41.0 33.0 41.0 42-43 37.722687370424225 40.0 37.0 41.0 33.0 41.0 44-45 37.533760863090215 40.0 37.0 41.0 33.0 41.0 46-47 37.27198118702782 40.0 37.0 41.0 32.0 41.0 48-49 37.0280044340042 40.0 37.0 41.0 31.0 41.0 50-51 36.81166479104279 40.0 36.0 41.0 31.0 41.0 52-53 36.71248681316585 40.0 36.0 41.0 31.0 41.0 54-55 36.58647415967404 39.5 36.0 41.0 31.0 41.0 56-57 36.41646091048212 39.0 35.0 41.0 30.5 41.0 58-59 36.24034819111116 39.0 35.0 41.0 30.0 41.0 60-61 36.051776389441144 39.0 35.0 41.0 30.0 41.0 62-63 35.85279891013292 39.0 35.0 41.0 30.0 41.0 64-65 35.576367652604134 38.0 35.0 40.0 29.0 41.0 66-67 35.32172485991511 37.5 35.0 40.0 29.0 41.0 68-69 35.0158887596086 37.0 34.5 40.0 29.0 41.0 70-71 34.72590906623276 36.5 34.0 39.5 29.0 41.0 72-73 34.405524091373934 36.0 34.0 39.0 28.0 41.0 74-75 34.06705727084253 36.0 34.0 39.0 28.0 40.5 76-77 33.77702541702675 35.0 34.0 38.0 28.0 40.0 78-79 33.4541963110611 35.0 34.0 37.0 28.0 39.0 80-81 33.149657839251944 35.0 34.0 37.0 27.0 39.0 82-83 32.85712609914738 35.0 33.5 36.0 27.0 39.0 84-85 32.58633088352889 35.0 33.0 36.0 26.5 37.0 86-87 32.31516386888141 35.0 33.0 36.0 26.0 37.0 88-89 32.111659729059156 35.0 33.0 35.0 26.0 37.0 90-91 31.965936371599483 35.0 33.0 35.0 26.0 36.0 92-93 31.809754295716683 35.0 33.0 35.0 26.0 36.0 94-95 31.630170449460127 35.0 33.0 35.0 25.0 36.0 96-97 31.46406138352082 35.0 33.0 35.0 25.0 36.0 98-99 31.284566011934544 35.0 33.0 35.0 25.0 35.0 100 31.174517680995258 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36830687162769493 1101 2 0.3993339277606509 1101 3 0.47606157001364124 1101 4 0.37717162859865283 1101 5 0.30628162283924354 1101 6 0.275921321093449 1101 7 0.32046671243873703 1101 8 0.3135650074647742 1101 9 0.32363766870692245 1101 10-11 0.3560111849821297 1101 12-13 0.31931173921546474 1101 14-15 0.4651266938128913 1101 16-17 0.5084414370708856 1101 18-19 0.463224133995773 1101 20-21 0.4203816659944053 1101 22-23 0.4644585236330343 1101 24-25 0.46802432706397923 1101 26-27 0.4544446235833348 1101 28-29 0.44769474194650627 1101 30-31 0.41060706845125594 1101 32-33 0.504375317565227 1101 34-35 0.5071243912676877 1101 36-37 0.47739726268432037 1101 38-39 0.5194351296826625 1101 40-41 0.4941882594716702 1101 42-43 0.4508663193041258 1101 44-45 0.49293191748927967 1101 46-47 0.5578767607413013 1101 48-49 0.5660527052647737 1101 50-51 0.5757183095287317 1101 52-53 0.5630736286365945 1101 54-55 0.49402514219926985 1101 56-57 0.46470816907703494 1101 58-59 0.4699899217843395 1101 60-61 0.5360771876661801 1101 62-63 0.5404306122933704 1101 64-65 0.4987236393290715 1101 66-67 0.5234684104479044 1101 68-69 0.5170114737724134 1101 70-71 0.5366687447358984 1101 72-73 0.5598694659755097 1101 74-75 0.5365018798376298 1101 76-77 0.48628899521656166 1101 78-79 0.486279513735731 1101 80-81 0.4467281286963498 1101 82-83 0.4736473789754214 1101 84-85 0.4355399977197223 1101 86-87 0.532883053780111 1101 88-89 0.5163560415022559 1101 90-91 0.5307342368874721 1101 92-93 0.5320878901689348 1101 94-95 0.4737838700713084 1101 96-97 0.5084975002160625 1101 98-99 0.5209971526668902 1101 100 0.5842103158585275 1102 1 0.2920738258100499 1102 2 0.3403174638873736 1102 3 0.3771900932548249 1102 4 0.36596439420354443 1102 5 0.28747682767863125 1102 6 0.24362985865407438 1102 7 0.28393675075405156 1102 8 0.27673348906921547 1102 9 0.34669204181899005 1102 10-11 0.33717915648630026 1102 12-13 0.2666158843744313 1102 14-15 0.3732999711013463 1102 16-17 0.3994371423275709 1102 18-19 0.3688131831309249 1102 20-21 0.3620180879387078 1102 22-23 0.382766080073921 1102 24-25 0.3230094558637475 1102 26-27 0.3349697357170669 1102 28-29 0.35634869986880346 1102 30-31 0.41126299109920694 1102 32-33 0.4372537224409001 1102 34-35 0.45134370014397973 1102 36-37 0.5136052549239878 1102 38-39 0.44433199739658136 1102 40-41 0.50937937369509 1102 42-43 0.5098498523776627 1102 44-45 0.5604396589532783 1102 46-47 0.565609615843826 1102 48-49 0.6585559269219843 1102 50-51 0.6737011508373882 1102 52-53 0.5346113694049066 1102 54-55 0.5230290094964332 1102 56-57 0.6101081125494616 1102 58-59 0.5441654305161236 1102 60-61 0.5251780416207055 1102 62-63 0.557597738064814 1102 64-65 0.596876821196723 1102 66-67 0.6131940499978441 1102 68-69 0.5452866598914028 1102 70-71 0.5447970529455048 1102 72-73 0.6067247327117187 1102 74-75 0.5682831329657745 1102 76-77 0.5564354696691325 1102 78-79 0.5124894316638944 1102 80-81 0.4845759602686073 1102 82-83 0.4713099519002384 1102 84-85 0.5184919189701773 1102 86-87 0.5368548735208378 1102 88-89 0.5131242760602746 1102 90-91 0.48680068448072333 1102 92-93 0.5290022142599078 1102 94-95 0.529110023472569 1102 96-97 0.611341352831758 1102 98-99 0.6470913602700676 1102 100 0.7207149033806317 1103 1 0.2974166337556525 1103 2 0.3676708483402109 1103 3 0.4339726638311987 1103 4 0.3563746023269374 1103 5 0.24233843739136063 1103 6 0.27206868684984187 1103 7 0.27176151323953945 1103 8 0.2829099612675634 1103 9 0.32159006038725124 1103 10-11 0.3302393763796161 1103 12-13 0.3070177124813398 1103 14-15 0.3541022481918503 1103 16-17 0.3493968213939951 1103 18-19 0.3870033248626896 1103 20-21 0.3823460392520772 1103 22-23 0.3519768572804409 1103 24-25 0.3523650852345739 1103 26-27 0.3715521186484523 1103 28-29 0.41369288946435745 1103 30-31 0.4191011068672381 1103 32-33 0.3596872792273089 1103 34-35 0.39633814575114457 1103 36-37 0.42566546723739407 1103 38-39 0.35973809973300774 1103 40-41 0.3668495793676385 1103 42-43 0.349584142689217 1103 44-45 0.37220529684308445 1103 46-47 0.3657502337837073 1103 48-49 0.3618090261594844 1103 50-51 0.3709612118051666 1103 52-53 0.36516077212870357 1103 54-55 0.3364650183851481 1103 56-57 0.38062735142499093 1103 58-59 0.25532284344080836 1103 60-61 0.252084190068274 1103 62-63 0.2873392271895341 1103 64-65 0.33071263267077455 1103 66-67 0.37010241557037205 1103 68-69 0.3455865613103981 1103 70-71 0.3991553458140231 1103 72-73 0.4190298920646782 1103 74-75 0.40269680763669413 1103 76-77 0.35110801105020073 1103 78-79 0.3475621798924067 1103 80-81 0.31301551487283774 1103 82-83 0.35677566605240685 1103 84-85 0.3723671712655303 1103 86-87 0.44324294113536666 1103 88-89 0.4424401635187607 1103 90-91 0.4186245234799735 1103 92-93 0.43235852666523655 1103 94-95 0.45892424819260924 1103 96-97 0.4433630486523228 1103 98-99 0.43956066266948923 1103 100 0.4627958720263159 1104 1 0.2504834747032021 1104 2 0.2539873814708926 1104 3 0.3493316301838405 1104 4 0.2550708290991395 1104 5 0.22051124996302462 1104 6 0.21610113565324696 1104 7 0.15623178737089205 1104 8 0.2220052990740058 1104 9 0.23833388906373187 1104 10-11 0.22286450210675213 1104 12-13 0.24910249074392254 1104 14-15 0.34346285647527 1104 16-17 0.3317052711824431 1104 18-19 0.35435367011547925 1104 20-21 0.3809646036379277 1104 22-23 0.3551252860281906 1104 24-25 0.34420274805922446 1104 26-27 0.37378998082633075 1104 28-29 0.33787480542393666 1104 30-31 0.368358128978052 1104 32-33 0.3696142765640573 1104 34-35 0.2753292365267157 1104 36-37 0.34831405353351386 1104 38-39 0.38447976144457385 1104 40-41 0.3572315779254751 1104 42-43 0.33382729871713224 1104 44-45 0.3540934654551009 1104 46-47 0.4557671126618814 1104 48-49 0.42616214234103467 1104 50-51 0.47853266783327797 1104 52-53 0.4602404565246232 1104 54-55 0.40781668988998376 1104 56-57 0.4483409309145472 1104 58-59 0.4949284539408936 1104 60-61 0.48557857240169255 1104 62-63 0.5426823606382882 1104 64-65 0.5210172528126336 1104 66-67 0.5044230784531649 1104 68-69 0.45278872540148996 1104 70-71 0.459017706274949 1104 72-73 0.474587522488207 1104 74-75 0.5206208001454158 1104 76-77 0.46861040287349454 1104 78-79 0.4706456783617057 1104 80-81 0.4473753716883522 1104 82-83 0.47332715346415455 1104 84-85 0.4428394577534718 1104 86-87 0.44622573387505327 1104 88-89 0.39864709471991233 1104 90-91 0.36984620418283853 1104 92-93 0.4646425419566107 1104 94-95 0.4082873026349816 1104 96-97 0.4288404974809126 1104 98-99 0.4977851110901419 1104 100 0.5259937003814166 1105 1 0.19673451283977528 1105 2 0.19449635559558232 1105 3 0.28104303352293414 1105 4 0.20247774191705048 1105 5 0.14743515010763275 1105 6 0.1791289004275498 1105 7 0.1752682548689819 1105 8 0.1623916257046787 1105 9 0.1959835718767451 1105 10-11 0.16550594586664857 1105 12-13 0.18035905934708296 1105 14-15 0.2729829598578277 1105 16-17 0.24342510394649963 1105 18-19 0.24761128342042582 1105 20-21 0.18544048865782514 1105 22-23 0.2531746389304885 1105 24-25 0.21645543838322823 1105 26-27 0.19181965338599127 1105 28-29 0.25388088051278146 1105 30-31 0.2633584919046328 1105 32-33 0.24112119052846737 1105 34-35 0.19549998128227486 1105 36-37 0.24551576274340903 1105 38-39 0.22506565024712444 1105 40-41 0.31167186360485744 1105 42-43 0.27004492746718256 1105 44-45 0.29290159403445415 1105 46-47 0.2853102533611178 1105 48-49 0.3720257553839943 1105 50-51 0.3673561714079696 1105 52-53 0.3710458605207876 1105 54-55 0.3220983553503771 1105 56-57 0.33616841986465573 1105 58-59 0.3271400249156642 1105 60-61 0.34289690169687503 1105 62-63 0.31476072516330333 1105 64-65 0.3109891856511453 1105 66-67 0.34752084913112924 1105 68-69 0.36683757268547623 1105 70-71 0.3916564025218179 1105 72-73 0.31612841753565135 1105 74-75 0.316995825931798 1105 76-77 0.34571249628978507 1105 78-79 0.2613209073327454 1105 80-81 0.27930341837566175 1105 82-83 0.3165481187373089 1105 84-85 0.33544972449327304 1105 86-87 0.37651821822719 1105 88-89 0.33050275847003263 1105 90-91 0.30148623744813463 1105 92-93 0.27783173934040306 1105 94-95 0.331489781892202 1105 96-97 0.41793773465292716 1105 98-99 0.44947920550388787 1105 100 0.4753414957515574 1106 1 0.19731939438640467 1106 2 0.19485640635784307 1106 3 0.31411352406751547 1106 4 0.2345495037612082 1106 5 0.20110589211576269 1106 6 0.23111282274601308 1106 7 0.22196541064366215 1106 8 0.23287886175374695 1106 9 0.19656946280667142 1106 10-11 0.24933607491892218 1106 12-13 0.18341461681340832 1106 14-15 0.3075028407087288 1106 16-17 0.32599026543324783 1106 18-19 0.2726394704491355 1106 20-21 0.22886353678904214 1106 22-23 0.2175592594281639 1106 24-25 0.28219842337490064 1106 26-27 0.2871377420282357 1106 28-29 0.2911483307500049 1106 30-31 0.3312161722984399 1106 32-33 0.29504317084428777 1106 34-35 0.2882313990403844 1106 36-37 0.29574805455268205 1106 38-39 0.33870954135096554 1106 40-41 0.38462516792577617 1106 42-43 0.3829258509676663 1106 44-45 0.38184229149438664 1106 46-47 0.4372897932737416 1106 48-49 0.4213974030631604 1106 50-51 0.4745395560915 1106 52-53 0.42801796352154753 1106 54-55 0.3706060784037106 1106 56-57 0.4336193307508367 1106 58-59 0.41981421157515797 1106 60-61 0.3739081942286475 1106 62-63 0.41130413036497515 1106 64-65 0.407446559618613 1106 66-67 0.4363645102750269 1106 68-69 0.37711424427469353 1106 70-71 0.4203511884140525 1106 72-73 0.4280975828571627 1106 74-75 0.3953213532429771 1106 76-77 0.3935057348497537 1106 78-79 0.39938486941044005 1106 80-81 0.3293908262965388 1106 82-83 0.3765561616529993 1106 84-85 0.3884051782398572 1106 86-87 0.4477588934881709 1106 88-89 0.43447691175258996 1106 90-91 0.39425954928633544 1106 92-93 0.38063931656402517 1106 94-95 0.3595069444738712 1106 96-97 0.4131652909591317 1106 98-99 0.4266669839955419 1106 100 0.4922759661389797 1107 1 0.16008661585914297 1107 2 0.17422607034277604 1107 3 0.23479193349988492 1107 4 0.23142106122005401 1107 5 0.15834638828160053 1107 6 0.16249696958017523 1107 7 0.15681318318805637 1107 8 0.16354471890370093 1107 9 0.10673369261503751 1107 10-11 0.1475803284161188 1107 12-13 0.11856744979735367 1107 14-15 0.13617475849400051 1107 16-17 0.12267084551022833 1107 18-19 0.12600902048841078 1107 20-21 0.17319468951519923 1107 22-23 0.2156076600368877 1107 24-25 0.22471246674146528 1107 26-27 0.19151103529580382 1107 28-29 0.248174564085474 1107 30-31 0.22435673408517687 1107 32-33 0.2083997364683654 1107 34-35 0.17960098028724758 1107 36-37 0.21376435420390294 1107 38-39 0.21418624122983942 1107 40-41 0.19671144484735237 1107 42-43 0.1958918435813075 1107 44-45 0.16844319785585782 1107 46-47 0.17130467234716207 1107 48-49 0.16488626026593067 1107 50-51 0.228651968621449 1107 52-53 0.17183954941527446 1107 54-55 0.17819123573069362 1107 56-57 0.1913644386866693 1107 58-59 0.17173804327024556 1107 60-61 0.15473809820855422 1107 62-63 0.16132878815150775 1107 64-65 0.1990652961588779 1107 66-67 0.14920745443154715 1107 68-69 0.18699082642540077 1107 70-71 0.20943848110083252 1107 72-73 0.23598896683981962 1107 74-75 0.16230109831102624 1107 76-77 0.1880686517844481 1107 78-79 0.1686588870392427 1107 80-81 0.16600720717064377 1107 82-83 0.14596889571686944 1107 84-85 0.16795021491174822 1107 86-87 0.19105486848940956 1107 88-89 0.16759259751668054 1107 90-91 0.10558747926014078 1107 92-93 0.11111333297057158 1107 94-95 0.1610341243700084 1107 96-97 0.1509444851320012 1107 98-99 0.2151253471781338 1107 100 0.16743114927441738 1108 1 0.11266098874796171 1108 2 0.11928754332387115 1108 3 0.22757505772560904 1108 4 0.18884951382374027 1108 5 0.1371962011853185 1108 6 0.16886471607176645 1108 7 0.10157685067026989 1108 8 0.13326081863758787 1108 9 0.10591218331525454 1108 10-11 0.13892924181847377 1108 12-13 0.13210037719100853 1108 14-15 0.15316351719623356 1108 16-17 0.1654650459487641 1108 18-19 0.16754629123903442 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-0.6718525942471345 2215 26-27 -0.5600042578961109 2215 28-29 -0.540184904557492 2215 30-31 -0.49611493354154845 2215 32-33 -0.5492651148555439 2215 34-35 -0.6484676287108968 2215 36-37 -0.6703909556663064 2215 38-39 -0.6692641175603455 2215 40-41 -0.640310868646786 2215 42-43 -0.6437393451462228 2215 44-45 -0.7694364218170406 2215 46-47 -0.6948571465280722 2215 48-49 -0.7264269654092814 2215 50-51 -0.6558865568607359 2215 52-53 -0.8070831753611785 2215 54-55 -0.734720706962463 2215 56-57 -0.7083337082640568 2215 58-59 -0.6689068204643718 2215 60-61 -0.8540510139691619 2215 62-63 -0.838227202200251 2215 64-65 -0.7318101203207021 2215 66-67 -0.6399335152992478 2215 68-69 -0.5967493736298621 2215 70-71 -0.6855945750744326 2215 72-73 -0.5421264003929167 2215 74-75 -0.6006183082529262 2215 76-77 -0.6689506114712245 2215 78-79 -0.6062857318235615 2215 80-81 -0.6566563684831621 2215 82-83 -0.6151892970795743 2215 84-85 -0.5508465904927462 2215 86-87 -0.5605860804131169 2215 88-89 -0.6064060749630791 2215 90-91 -0.5803995370352055 2215 92-93 -0.694979175312465 2215 94-95 -0.6954967282725981 2215 96-97 -0.7630054158773731 2215 98-99 -0.7137520258843573 2215 100 -0.7516441227108963 2216 1 -0.6255404534531195 2216 2 -1.0470254858017647 2216 3 -0.7256462825922156 2216 4 -1.1663877755973502 2216 5 -0.6501460984594729 2216 6 -0.8185837147982156 2216 7 -0.5173485693991964 2216 8 -0.5395912441956341 2216 9 -0.5422448473640031 2216 10-11 -0.8277905159690206 2216 12-13 -0.6466049562048966 2216 14-15 -0.9502955512841709 2216 16-17 -0.8351749639416255 2216 18-19 -0.8962921806413817 2216 20-21 -0.7212126928515445 2216 22-23 -0.6983765363230034 2216 24-25 -0.8216075044422979 2216 26-27 -0.9319444981988028 2216 28-29 -1.1710000551460737 2216 30-31 -1.2714478733589871 2216 32-33 -1.006539155437288 2216 34-35 -0.9842044071227534 2216 36-37 -1.0150468369356531 2216 38-39 -1.1739987473697653 2216 40-41 -0.9481501205243177 2216 42-43 -1.5928846104880563 2216 44-45 -1.2140988522341232 2216 46-47 -1.0350422776095485 2216 48-49 -1.048566749171158 2216 50-51 -1.2127422670867531 2216 52-53 -1.1241282325053348 2216 54-55 -1.3643478322282334 2216 56-57 -1.4895271430127863 2216 58-59 -1.2622536518463505 2216 60-61 -1.4806523833647702 2216 62-63 -1.4308657475069495 2216 64-65 -1.3787140249027416 2216 66-67 -1.2834771542410408 2216 68-69 -1.598474301486462 2216 70-71 -1.3106084140473229 2216 72-73 -1.1501946385431765 2216 74-75 -1.2751762726757505 2216 76-77 -1.3984170132801523 2216 78-79 -1.4965488076860787 2216 80-81 -1.2228063399972022 2216 82-83 -1.2788725221747725 2216 84-85 -1.365811549008427 2216 86-87 -1.5831015045774564 2216 88-89 -1.4193918299997215 2216 90-91 -1.2356367005284419 2216 92-93 -1.2930816873358886 2216 94-95 -1.4072319504822026 2216 96-97 -1.444088464882718 2216 98-99 -1.461058688876701 2216 100 -1.4129709658671707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 435.0 3 42.0 4 74.0 5 144.0 6 442.0 7 1215.0 8 2462.0 9 3873.0 10 5660.0 11 7968.0 12 11507.0 13 16289.0 14 19065.0 15 24568.0 16 32458.0 17 46500.0 18 47468.0 19 25678.0 20 18952.0 21 18515.0 22 19084.0 23 20555.0 24 23624.0 25 29097.0 26 37237.0 27 48887.0 28 64329.0 29 84043.0 30 109168.0 31 140439.0 32 180833.0 33 235590.0 34 321332.0 35 466897.0 36 751932.0 37 1372589.0 38 1887855.0 39 736221.0 40 2483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9361625192158 20.376166436148058 14.893327458913685 22.79434358572246 2 15.648513071178586 20.650617835164038 40.00473333469298 23.696135758964395 3 18.087438517064534 25.52204098035818 30.92775609275844 25.46276440981884 4 12.751997781206123 17.648812995221896 37.73486177049292 31.86432745307906 5 12.654394758461091 37.633299160739284 35.333875340900605 14.37843073989902 6 30.230058329648053 37.98974733992214 17.448992737332294 14.331201593097518 7 26.241425806726127 33.24462879520388 22.37471590533944 18.139229492730554 8 24.89963333631672 36.15512265406404 20.215141640170728 18.73010236944851 9 24.7553227140222 16.825612999060375 21.884179433139835 36.53488485377759 10-11 23.747966036290755 27.13577560593411 28.475015075907745 20.64124328186739 12-13 24.62145335472083 24.724681177447167 29.039033410204595 21.614832057627403 14-15 21.793012347722282 26.621427192221038 27.063646612989086 24.5219138470676 16-17 20.533312987582732 29.837906480953002 27.858766255203204 21.77001427626106 18-19 20.412852449779987 27.946441278789113 31.040222962038055 20.600483309392843 20-21 21.40641712799189 27.135753597309666 31.02142759676092 20.43640167793753 22-23 21.158167180445776 27.012453946953347 31.0798238136251 20.749555058975776 24-25 20.985296771628242 27.09298350380236 31.04775724780684 20.87396247676256 26-27 20.75823379321577 27.122893224424878 31.06138792254725 21.057485059812105 28-29 20.765914965390266 27.13523910330511 31.234229411493263 20.864616519811364 30-31 21.07531365497465 27.395093573969476 30.862503586499017 20.667089184556854 32-33 20.796427618507465 27.237992992306737 31.10391820303383 20.861661186151967 34-35 20.931727779725946 27.051915410585565 31.054660619674827 20.961696190013658 36-37 21.24361933296261 27.223590017474848 30.63423720308532 20.898553446477226 38-39 21.062004164031745 27.322819568895063 30.723115364807622 20.89206090226557 40-41 21.029820218883106 27.171121456794868 30.732219599120242 21.066838725201784 42-43 21.024750899052307 27.267423861163724 30.573067899540902 21.134757340243063 44-45 21.199389334033697 27.43073519076342 30.29220850677352 21.077666968429362 46-47 21.282596606857005 27.595997951730684 29.865079795936033 21.256325645476274 48-49 21.38796656449774 27.591310114723623 29.59261302529084 21.428110295487794 50-51 21.44220315134157 27.66555987739729 29.364009443167127 21.52822752809401 52-53 21.371533458244503 27.808667289755277 29.289495577000107 21.53030367500011 54-55 21.459751361233423 28.057056625256216 29.135413197251562 21.347778816258796 56-57 21.31951974246975 27.846668847965887 29.203309803668397 21.63050160589596 58-59 21.220495838860256 27.51843389931524 29.49109166670885 21.76997859511565 60-61 21.408874757721726 27.724829103031173 29.265667572932912 21.600628566314185 62-63 21.215286897092074 27.75911853991851 29.369078762997923 21.656515799991492 64-65 21.24170458263578 27.556228367356223 29.475813255354332 21.726253794653665 66-67 21.173426493395212 27.583262294384426 29.37075141845585 21.872559793764516 68-69 21.450590010915548 27.744066676159722 29.158003521233443 21.647339791691287 70-71 21.369486656171 27.567709533109042 29.207726200973955 21.855077609746008 72-73 21.25402207611756 27.6895859590845 29.246131250632747 21.810260714165192 74-75 21.356611610869912 27.66831095545308 29.07356896255746 21.90150847111955 76-77 21.358988542310115 27.72730874138546 28.896421544389195 22.017281171915233 78-79 21.399494857458777 27.727568710272553 29.015654312771094 21.857282119497583 80-81 21.26390390473226 27.64997040196617 29.02814054433238 22.057985148969188 82-83 21.161587009349002 27.686991067108053 29.129015891163153 22.02240603237979 84-85 21.238557349339963 27.566997920918613 29.016067763087428 22.178376966654 86-87 21.305690990109323 27.654628927255626 28.906633546132277 22.13304653650277 88-89 21.157770747074718 27.542809386189106 28.951113366100785 22.34830650063539 90-91 21.026883780486653 27.493075163118906 28.940671808032963 22.539369248361474 92-93 21.10750909090509 27.552057889516128 28.87937735140762 22.461055668171163 94-95 20.991631022870987 27.37190407379918 28.99929770051758 22.63716720281225 96-97 20.99310249709853 27.386571217707846 28.979504101673975 22.64082218351965 98-99 20.969389151227674 27.390971634765037 28.87931986292732 22.760319351079968 100 21.00264443791352 27.316531573837448 28.838987140516448 22.84183684773258 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1181.0 1 1016.5 2 2140.5 3 3445.5 4 2244.5 5 1187.5 6 2539.0 7 6634.5 8 10962.5 9 12380.5 10 9629.0 11 6071.5 12 5515.0 13 5930.5 14 6506.5 15 7245.5 16 7981.5 17 8998.0 18 10095.5 19 11250.5 20 12944.0 21 15406.5 22 19266.0 23 24970.5 24 32415.5 25 41397.5 26 53068.5 27 67904.5 28 86506.0 29 105176.5 30 121078.0 31 137711.5 32 153673.5 33 167873.5 34 181812.5 35 194995.0 36 205567.5 37 215981.0 38 225320.0 39 231249.5 40 236312.0 41 237821.0 42 237501.0 43 238491.0 44 236177.5 45 231603.5 46 227276.5 47 221401.5 48 215232.5 49 212640.0 50 209131.0 51 200272.0 52 188522.0 53 179249.0 54 172251.0 55 161834.5 56 147956.5 57 132195.5 58 118779.5 59 108583.0 60 95508.5 61 79965.5 62 67438.5 63 55903.0 64 44828.0 65 35775.0 66 28137.0 67 21584.5 68 16146.5 69 12068.5 70 8720.0 71 6214.5 72 4569.5 73 3211.5 74 2148.5 75 1378.0 76 811.5 77 492.5 78 309.5 79 171.0 80 93.5 81 41.0 82 24.5 83 13.5 84 11.5 85 7.5 86 4.0 87 4.5 88 1.5 89 0.0 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0595259929190919 2 2.787759096531294E-4 3 1.4672416297533127E-5 4 0.12043119297015191 5 0.0015699485438360444 6 0.0015846209601335776 7 0.0 8 0.0 9 2.9344832595066254E-5 10-11 0.0 12-13 3.668104074383282E-5 14-15 2.9344832595066254E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.1275003631423034E-4 30-31 2.8611211780189594E-4 32-33 7.336208148766564E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.393879548265647E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.336208148766564E-6 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.1737933038026502E-4 80-81 3.4480178299202847E-4 82-83 3.5213799114079507E-4 84-85 0.0 86-87 0.0 88-89 1.0270691408273189E-4 90-91 9.537070593396531E-5 92-93 6.529225252402242E-4 94-95 6.089052763476248E-4 96-97 0.0 98-99 2.2008624446299692E-5 100 0.0010710863897199184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6815510.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.11121384610026 #Duplication Level Percentage of deduplicated Percentage of total 1 74.860655708258 43.50243572521867 2 14.992704757748188 17.424885446178994 3 4.594719258576065 8.01014140193727 4 1.9443429343070897 4.519525121826935 5 1.0016644892769555 2.9103969669208984 6 0.5907777638035716 2.0598487780746155 7 0.39074189030303874 1.5894539882220535 8 0.28378548730351544 1.3192895311291486 9 0.20545993040457827 1.0745573357290792 >10 1.0491131775289688 10.77209672945706 >50 0.05919398080839384 2.3647379318367068 >100 0.025553817821280543 2.5426016325283567 >500 7.569640510097487E-4 0.2982946891928443 >1k 4.289179418146369E-4 0.5104188293340498 >5k 2.5230466906876498E-5 0.10491934402063735 >10k+ 7.56914007206295E-5 0.9963965483927466 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26329 0.3863100486977497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25596 0.3755551675516579 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16034 0.23525752291464613 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7156 0.10499581102514706 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.4672416297533127E-5 0.0 0.0 0.0 3 0.0 1.4672416297533127E-5 0.0 0.0 0.0 4 0.0 1.4672416297533127E-5 0.0 0.0 0.0 5 0.0 1.4672416297533127E-5 0.0 0.0 0.0 6 0.0 1.4672416297533127E-5 0.0 0.0 0.0 7 0.0 1.4672416297533127E-5 0.0 0.0 0.0 8 0.0 1.4672416297533127E-5 0.0 0.0 0.0 9 5.868966519013251E-5 1.4672416297533127E-5 0.0 0.0 0.0 10-11 7.336208148766563E-5 1.4672416297533127E-5 0.0 7.336208148766564E-6 0.0 12-13 7.336208148766563E-5 1.4672416297533127E-5 0.0 2.2008624446299692E-5 0.0 14-15 8.803449778519875E-5 1.4672416297533127E-5 0.0 4.401724889259938E-5 0.0 16-17 8.803449778519875E-5 1.4672416297533127E-5 0.0 4.401724889259938E-5 0.0 18-19 1.0270691408273189E-4 1.4672416297533127E-5 0.0 5.868966519013251E-5 0.0 20-21 1.1737933038026502E-4 1.4672416297533127E-5 0.0 1.0270691408273189E-4 0.0 22-23 1.1737933038026502E-4 1.4672416297533127E-5 0.0 2.4943107705806315E-4 0.0 24-25 1.1737933038026502E-4 1.4672416297533127E-5 0.0 5.355431948599591E-4 0.0 26-27 1.3205174667779815E-4 1.4672416297533127E-5 0.0 8.950173941495207E-4 0.0 28-29 1.3205174667779815E-4 1.4672416297533127E-5 0.0 0.0026336987254071964 0.0 30-31 1.3205174667779815E-4 1.4672416297533127E-5 0.0 0.007754372013246257 0.0 32-33 1.3205174667779815E-4 1.4672416297533127E-5 0.0 0.01941160676163633 0.0 34-35 1.3205174667779815E-4 1.4672416297533127E-5 0.0 0.03908731701662825 0.0 36-37 1.3205174667779815E-4 2.2008624446299692E-5 0.0 0.07035423614667134 0.0 38-39 1.3205174667779815E-4 2.9344832595066254E-5 0.0 0.11254476921022785 0.0 40-41 1.3205174667779815E-4 2.9344832595066254E-5 0.0 0.15747904412142305 0.0 42-43 1.3205174667779815E-4 2.9344832595066254E-5 0.0 0.20421069002906606 0.0 44-45 1.3205174667779815E-4 2.9344832595066254E-5 0.0 0.264771088297134 0.0 46-47 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.3393069630886023 0.0 48-49 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.4192496232857116 0.0 50-51 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.5013197838459631 0.0 52-53 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.5853707206063816 0.0 54-55 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.6844021944065815 0.0 56-57 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.8058531203094119 0.0 58-59 1.613965792728644E-4 2.9344832595066254E-5 0.0 0.9420791694238582 0.0 60-61 1.613965792728644E-4 4.401724889259938E-5 0.0 1.0843062368039955 0.0 62-63 1.613965792728644E-4 4.401724889259938E-5 0.0 1.23184471888384 0.0 64-65 1.613965792728644E-4 4.401724889259938E-5 0.0 1.3956769192620948 0.0 66-67 1.613965792728644E-4 4.401724889259938E-5 0.0 1.5922359441919975 0.0 68-69 1.613965792728644E-4 4.401724889259938E-5 0.0 1.8146037493892608 0.0 70-71 1.613965792728644E-4 4.401724889259938E-5 0.0 2.053668764333117 0.0 72-73 1.613965792728644E-4 7.336208148766563E-5 0.0 2.3061737126055126 0.0 74-75 1.613965792728644E-4 7.336208148766563E-5 0.0 2.5690740678247117 0.0 76-77 1.613965792728644E-4 7.336208148766563E-5 0.0 2.858663548289123 0.0 78-79 1.7606899557039753E-4 7.336208148766563E-5 0.0 3.190384872151901 0.0 80-81 1.9074141186793065E-4 7.336208148766563E-5 0.0 3.5535418479321432 0.0 82-83 2.0541382816546377E-4 7.336208148766563E-5 0.0 3.9306302829868933 0.0 84-85 2.1275003631423032E-4 7.336208148766563E-5 0.0 4.312597296460574 0.0 86-87 2.200862444629969E-4 7.336208148766563E-5 0.0 4.724730797841981 0.0 88 2.200862444629969E-4 7.336208148766563E-5 0.0 5.05894643247534 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13910 0.0 57.09505 1 GTATCAA 25020 0.0 40.92441 1 GTGGTAT 5365 0.0 32.467026 1 ATCAACG 31340 0.0 32.27783 3 TCAACGC 31755 0.0 31.900446 4 TATCAAC 32290 0.0 31.48847 2 CAACGCA 32880 0.0 30.818165 5 AACGCAG 34330 0.0 29.652744 6 TGGTATC 6180 0.0 27.179693 2 ACGCAGA 39440 0.0 25.631699 7 CGCAGAG 39765 0.0 25.280384 8 GCAGAGT 42445 0.0 23.329845 9 GTACATG 20865 0.0 22.653006 1 TACATGG 21115 0.0 21.413967 2 CAGAGTA 41485 0.0 20.83361 10-11 GAGTACT 30785 0.0 20.830912 12-13 AGAGTAC 40120 0.0 20.341722 10-11 ACATGGG 21650 0.0 20.080704 3 AGTACTT 31725 0.0 19.510035 12-13 GTACTTT 33330 0.0 19.035852 14-15 >>END_MODULE