FastQCFastQC Report
Wed 25 May 2016
SRR2049527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358131
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87900.048.8556371
GTATCAA148500.038.8108861
ATCAACG167100.033.3207443
TCAACGC167050.033.1775474
CAACGCA182900.030.3579465
TATCAAC187400.030.3393842
AACGCAG200250.027.8291536
GTACATG288000.025.9123421
TACATGG289100.024.959352
GTGGTAT47350.024.538471
ACGCAGA228950.023.9381457
ACATGGG299150.023.368133
CGCAGAG230650.023.3205818
TGGTATC52800.020.4508252
GCAGAGT252100.020.2237669
CATGGGG180950.019.3728834
CATGGGT81900.019.307984
GAGTACA220400.019.0898191
AGAGTAC222750.017.44567110-11
CAGAGTA242700.017.17662610-11
Produced by FastQC (version 0.11.5)