##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8358131 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.696836290314188 31.0 31.0 34.0 27.0 34.0 2 30.92476691260283 31.0 31.0 34.0 27.0 34.0 3 31.012856462766617 31.0 31.0 34.0 27.0 34.0 4 34.57764409291982 37.0 35.0 37.0 32.0 37.0 5 34.56884679122641 37.0 35.0 37.0 32.0 37.0 6 34.608526714883986 37.0 35.0 37.0 31.0 37.0 7 30.669906226643253 37.0 33.0 37.0 2.0 37.0 8 32.44781650347428 37.0 33.0 37.0 17.0 37.0 9 35.18124327077429 39.0 34.0 39.0 28.0 39.0 10-11 35.711219769108666 39.0 36.0 39.0 30.0 39.0 12-13 35.848352281149936 39.0 37.0 39.0 30.0 39.0 14-15 37.024693319595016 40.0 37.0 41.0 31.0 41.0 16-17 37.044595077535874 40.0 37.0 41.0 31.0 41.0 18-19 37.05204770061632 40.0 37.0 41.0 30.5 41.0 20-21 37.12131922794701 40.0 37.0 41.0 31.0 41.0 22-23 37.09243603623825 40.0 37.0 41.0 30.5 41.0 24-25 37.07747832619518 40.0 37.0 41.0 30.0 41.0 26-27 37.10372839334535 40.0 37.0 41.0 31.0 41.0 28-29 37.094428168211294 40.0 37.0 41.0 31.0 41.0 30-31 37.02175217162784 40.0 37.0 41.0 30.0 41.0 32-33 36.97161596294674 40.0 37.0 41.0 30.0 41.0 34-35 36.91608829773068 40.0 37.0 41.0 30.0 41.0 36-37 36.753815296745174 40.0 37.0 41.0 30.0 41.0 38-39 36.64696030727444 40.0 36.0 41.0 30.0 41.0 40-41 36.570302798556284 40.0 36.0 41.0 30.0 41.0 42-43 36.46049553422888 40.0 36.0 41.0 30.0 41.0 44-45 36.35039741540304 40.0 36.0 41.0 30.0 41.0 46-47 36.19438173438536 40.0 35.5 41.0 29.0 41.0 48-49 36.11130801850318 40.0 35.0 41.0 29.0 41.0 50-51 35.973121801991375 39.0 35.0 41.0 29.0 41.0 52-53 35.85258372954432 39.0 35.0 41.0 28.0 41.0 54-55 35.72854355836251 39.0 35.0 41.0 28.0 41.0 56-57 35.52566674295964 39.0 35.0 41.0 28.0 41.0 58-59 35.337270557257355 39.0 35.0 41.0 28.0 41.0 60-61 35.11291346115537 38.5 34.0 40.0 27.5 41.0 62-63 34.86966601743859 38.0 34.0 40.0 27.0 41.0 64-65 34.50701652079873 37.5 34.0 40.0 26.0 41.0 66-67 31.037477397757947 36.0 30.0 40.0 6.5 41.0 68-69 31.392640053141065 36.0 30.0 39.0 11.0 41.0 70-71 31.547541310371898 35.0 30.0 39.0 15.5 41.0 72-73 32.76843937956943 35.0 31.5 39.0 23.5 40.5 74-75 32.846741095587035 35.0 33.0 38.5 24.0 40.0 76-77 32.548887125602604 35.0 33.0 37.0 23.5 39.5 78-79 29.20216876237044 35.0 29.0 36.5 6.5 39.0 80-81 30.972775851443345 35.0 29.5 36.0 18.5 39.0 82-83 31.367986814277018 35.0 31.5 36.0 20.0 37.5 84-85 29.547482146427235 35.0 30.0 35.5 11.0 37.0 86-87 29.181875110595897 34.0 28.0 35.0 10.5 37.0 88-89 30.40204197565221 34.0 30.5 35.0 17.5 36.0 90-91 30.53839895546026 35.0 31.5 35.0 16.0 36.0 92-93 30.384587834289746 35.0 32.0 35.0 8.5 36.0 94-95 30.175659247264733 35.0 31.5 35.0 2.0 36.0 96-97 29.98159546673772 35.0 31.0 35.0 2.0 35.0 98-99 29.786336562564045 35.0 31.0 35.0 2.0 35.0 100 29.56886485746634 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3538139760152461 1101 2 0.3752129692635364 1101 3 0.4048852845375137 1101 4 0.40732516314514555 1101 5 0.34730966485518877 1101 6 0.3626383059630385 1101 7 -12.024823802700602 1101 8 -6.457234814854214 1101 9 -2.164613795165515 1101 10-11 -0.13993084166843772 1101 12-13 0.37165127523945785 1101 14-15 0.5388423197756254 1101 16-17 0.6332072859498155 1101 18-19 0.6229345101277346 1101 20-21 0.6091938630675529 1101 22-23 0.5570801037345703 1101 24-25 0.6605245215745157 1101 26-27 0.5891655539956986 1101 28-29 0.5445337069359368 1101 30-31 0.6071388645931748 1101 32-33 0.6302206546640079 1101 34-35 0.5893333026227907 1101 36-37 0.6619093422485562 1101 38-39 0.675397401674843 1101 40-41 0.6759058960952231 1101 42-43 0.6454686972885426 1101 44-45 0.5610385668133375 1101 46-47 0.5747406476896089 1101 48-49 0.5263013440478588 1101 50-51 0.618951381740068 1101 52-53 0.6033288481822723 1101 54-55 0.5465075984369392 1101 56-57 0.5288286590860878 1101 58-59 0.5193222076907205 1101 60-61 0.4556393174282718 1101 62-63 0.5023629706492585 1101 64-65 0.3593969715923393 1101 66-67 -10.150287316175714 1101 68-69 -8.055500755565376 1101 70-71 -6.592483077814467 1101 72-73 -1.5022990720732707 1101 74-75 -0.21508624410687105 1101 76-77 0.09480057861068758 1101 78-79 3.230595971097994 1101 80-81 1.1357209739924805 1101 82-83 0.4275841339731308 1101 84-85 -5.246957162430331 1101 86-87 -5.748722650782739 1101 88-89 -1.2024739271983371 1101 90-91 0.06517207033041217 1101 92-93 0.41751611318639803 1101 94-95 0.45905636565963803 1101 96-97 0.5409977413937135 1101 98-99 0.5322360227598679 1101 100 0.5978856628743863 1102 1 0.25775754406097207 1102 2 0.3379388404515282 1102 3 0.3588077154075684 1102 4 0.3141694638052286 1102 5 0.33371025263940624 1102 6 0.357254243408093 1102 7 -12.206395089412492 1102 8 -6.582127418010124 1102 9 -2.222297286206839 1102 10-11 -0.16062760965783696 1102 12-13 0.38935670604165296 1102 14-15 0.5559890418352609 1102 16-17 0.5347827534009753 1102 18-19 0.5726053115674219 1102 20-21 0.5293163010879098 1102 22-23 0.49777555110649985 1102 24-25 0.5638319532945815 1102 26-27 0.5185384358091909 1102 28-29 0.5264805743364604 1102 30-31 0.5316744632597192 1102 32-33 0.5272492215897628 1102 34-35 0.5173894266761963 1102 36-37 0.675471244550593 1102 38-39 0.6481830581640011 1102 40-41 0.5497075889662995 1102 42-43 0.5638534070190104 1102 44-45 0.4978532739682464 1102 46-47 0.5770688160223756 1102 48-49 0.47812380344616656 1102 50-51 0.4888054717507302 1102 52-53 0.49032236402348417 1102 54-55 0.4824880036840895 1102 56-57 0.47954757842700246 1102 58-59 0.4614914860425898 1102 60-61 0.4271007667074471 1102 62-63 0.41790474849221226 1102 64-65 0.3116869003884375 1102 66-67 -10.25760547043405 1102 68-69 -6.6634168357976264 1102 70-71 -6.190182671633394 1102 72-73 -0.9819486414863121 1102 74-75 -0.08521722601149406 1102 76-77 0.08438335834263455 1102 78-79 3.1957467075428774 1102 80-81 1.1211418606156478 1102 82-83 0.46919140568689244 1102 84-85 -5.42020736982564 1102 86-87 -5.902885455920156 1102 88-89 -1.2975142789933187 1102 90-91 -0.018073044296993857 1102 92-93 0.33962753973781545 1102 94-95 0.3493687974855 1102 96-97 0.45613276176113615 1102 98-99 0.49032862795872134 1102 100 0.5600367955045087 1103 1 0.22048775006742716 1103 2 0.23741596463453618 1103 3 0.23332248334005712 1103 4 0.261633681559843 1103 5 0.24763595794262017 1103 6 0.2877398891431824 1103 7 -12.153695494806716 1103 8 -6.57348507499697 1103 9 -2.262171531263512 1103 10-11 -0.3388289830341904 1103 12-13 0.22712869247335732 1103 14-15 0.39638781737923523 1103 16-17 0.4099023033306466 1103 18-19 0.4605866137929553 1103 20-21 0.40977795410354645 1103 22-23 0.4438912125609562 1103 24-25 0.5324685049616562 1103 26-27 0.5039823011190308 1103 28-29 0.48295860858813455 1103 30-31 0.45500448725243814 1103 32-33 0.4980873121002318 1103 34-35 0.4879925540030783 1103 36-37 0.5382701193747792 1103 38-39 0.5407271137609229 1103 40-41 0.5374034605638869 1103 42-43 0.5172827112301519 1103 44-45 0.4702583372593807 1103 46-47 0.5310698088245545 1103 48-49 0.47736100043466934 1103 50-51 0.5024871131836761 1103 52-53 0.527492050463465 1103 54-55 0.5283678746198106 1103 56-57 0.49409543976562986 1103 58-59 0.5040926277651039 1103 60-61 0.46004277051318354 1103 62-63 0.3673363692417837 1103 64-65 0.27581199346364116 1103 66-67 -10.289639887576435 1103 68-69 -8.227531173515839 1103 70-71 -6.723333163897809 1103 72-73 -1.590070590687933 1103 74-75 -0.3851775139241198 1103 76-77 -0.07406125156883547 1103 78-79 3.035539873754505 1103 80-81 0.927410579073495 1103 82-83 0.2343862431155337 1103 84-85 -5.505557746263133 1103 86-87 -5.982575205237609 1103 88-89 -1.4667994614207203 1103 90-91 -0.19227011553054751 1103 92-93 0.25172648902217176 1103 94-95 0.3096525734431417 1103 96-97 0.43921872188358435 1103 98-99 0.4061737508179917 1103 100 0.5232882226576479 1104 1 0.13682564302237665 1104 2 0.21446621977132807 1104 3 0.19909128186659686 1104 4 0.20507374944087786 1104 5 0.19747486423732852 1104 6 0.2529598482373885 1104 7 -12.234292841647932 1104 8 -6.621470782056491 1104 9 -2.4202773170942464 1104 10-11 -0.3285159664336632 1104 12-13 0.22267709839440641 1104 14-15 0.3699136053207752 1104 16-17 0.42328701537702074 1104 18-19 0.45844594022897667 1104 20-21 0.4452781339136891 1104 22-23 0.4225020211728392 1104 24-25 0.4922571539139682 1104 26-27 0.46889035868162665 1104 28-29 0.39569357125215987 1104 30-31 0.3814657117378317 1104 32-33 0.4023957076776128 1104 34-35 0.43564256182961003 1104 36-37 0.49382300809682533 1104 38-39 0.4920074341847922 1104 40-41 0.5169508318768834 1104 42-43 0.4477326719439674 1104 44-45 0.4525309033720646 1104 46-47 0.453829792862976 1104 48-49 0.395087661355916 1104 50-51 0.4163828791577586 1104 52-53 0.43286853699771655 1104 54-55 0.3808999556113406 1104 56-57 0.35949098685220804 1104 58-59 0.31198904537706795 1104 60-61 0.3314057095466012 1104 62-63 0.24704755640122755 1104 64-65 0.18149194460146134 1104 66-67 -10.36056103996199 1104 68-69 -8.353305109149431 1104 70-71 -6.80217490567744 1104 72-73 -1.7611979654611112 1104 74-75 -0.528647676531989 1104 76-77 -0.19689233172617548 1104 78-79 2.941903400423147 1104 80-81 0.849794001929105 1104 82-83 0.20194949277404817 1104 84-85 -5.532405271437948 1104 86-87 -5.989254657822364 1104 88-89 -1.4746182311380167 1104 90-91 -0.2094276897329337 1104 92-93 0.178549646659377 1104 94-95 0.18790072956877069 1104 96-97 0.2529065291455552 1104 98-99 0.28886597918445744 1104 100 0.34022154552476636 1105 1 0.12734673708182598 1105 2 0.18232490821662495 1105 3 0.17645349147188227 1105 4 0.17790101704291317 1105 5 0.16246096876312066 1105 6 0.2399833871295698 1105 7 -12.089056567585374 1105 8 -6.520227852043732 1105 9 -2.381208691305879 1105 10-11 -0.35199541514076316 1105 12-13 0.19315472558169233 1105 14-15 0.36498098350179475 1105 16-17 0.3707090542073459 1105 18-19 0.4186748504789506 1105 20-21 0.42271344501115493 1105 22-23 0.4386733222121535 1105 24-25 0.4517207053335426 1105 26-27 0.4328839797408577 1105 28-29 0.4398914711755566 1105 30-31 0.4347297652142217 1105 32-33 0.37647644935669433 1105 34-35 0.3851625332952011 1105 36-37 0.46273920793984047 1105 38-39 0.43434662265789115 1105 40-41 0.4211606444887295 1105 42-43 0.3910031394267577 1105 44-45 0.332598898473222 1105 46-47 0.3464299818901537 1105 48-49 0.2922233248500419 1105 50-51 0.367883859362486 1105 52-53 0.3822378308593102 1105 54-55 0.3100955374893104 1105 56-57 0.33140343370504155 1105 58-59 0.30309010467126285 1105 60-61 0.22439817676981733 1105 62-63 0.1688195261790213 1105 64-65 0.05715516262049647 1105 66-67 -10.204544736795995 1105 68-69 -8.22107717986438 1105 70-71 -6.696010169915532 1105 72-73 -1.7179631319887179 1105 74-75 -0.5303875273296228 1105 76-77 -0.22988988340036087 1105 78-79 2.8913521147738273 1105 80-81 0.7904209699798912 1105 82-83 0.09809107220289803 1105 84-85 -5.514824401059823 1105 86-87 -5.988627223128091 1105 88-89 -1.618789391760881 1105 90-91 -0.35042881489287936 1105 92-93 1.7595938848558035E-4 1105 94-95 0.004857069425106886 1105 96-97 0.06354642855841774 1105 98-99 0.12660623442356211 1105 100 0.24879144675935905 1106 1 0.09910454313871142 1106 2 0.13150124914637473 1106 3 0.1631984718975268 1106 4 0.09216191850085664 1106 5 0.07871431541934726 1106 6 0.16644330327849843 1106 7 -12.240988158795652 1106 8 -6.743416398144966 1106 9 -2.5625703036974485 1106 10-11 -0.48385999637500987 1106 12-13 0.07538720316136249 1106 14-15 0.24761928557231982 1106 16-17 0.25849052998145083 1106 18-19 0.26515879932935604 1106 20-21 0.29096549565294083 1106 22-23 0.28550027207332107 1106 24-25 0.3571687265253658 1106 26-27 0.3062437730694896 1106 28-29 0.2774764593467296 1106 30-31 0.23203378880189973 1106 32-33 0.2881693693764831 1106 34-35 0.3677968182591229 1106 36-37 0.4170273371839599 1106 38-39 0.41223117971893686 1106 40-41 0.3890499440172519 1106 42-43 0.3149045914374966 1106 44-45 0.2485769378522633 1106 46-47 0.29884974114661134 1106 48-49 0.2711542671912639 1106 50-51 0.3272923827922014 1106 52-53 0.28634772277570875 1106 54-55 0.2656878716396136 1106 56-57 0.3054438138045441 1106 58-59 0.3236033055104244 1106 60-61 0.3016067085437655 1106 62-63 0.213842933435771 1106 64-65 0.06721947482950696 1106 66-67 -10.336952207141128 1106 68-69 -8.26990993138481 1106 70-71 -6.805423403323708 1106 72-73 -1.7396032493462599 1106 74-75 -0.5120378784817206 1106 76-77 -0.2384096401277489 1106 78-79 2.895275471330958 1106 80-81 0.7441697535247975 1106 82-83 0.12402918362548121 1106 84-85 -5.569147980541267 1106 86-87 -6.029890531173287 1106 88-89 -1.5529737035688562 1106 90-91 -0.27782574177469854 1106 92-93 0.05145971636019553 1106 94-95 0.1039529482488355 1106 96-97 0.190470948290816 1106 98-99 0.22481235420368506 1106 100 0.3394510017853456 1107 1 0.10227218495187884 1107 2 0.11326057000215073 1107 3 0.08620995935281428 1107 4 0.05948268808477053 1107 5 0.10302744973725453 1107 6 0.07425000966591 1107 7 -12.203550029000962 1107 8 -6.707964587836379 1107 9 -2.524229484439921 1107 10-11 -0.5353236496414056 1107 12-13 0.06819231130195647 1107 14-15 0.16275908777545567 1107 16-17 0.19961797232456746 1107 18-19 0.2131696856547194 1107 20-21 0.19562250192241493 1107 22-23 0.2546987798780975 1107 24-25 0.32475296242169804 1107 26-27 0.233046578034525 1107 28-29 0.1893023926533175 1107 30-31 0.21292594989054692 1107 32-33 0.2570664869317483 1107 34-35 0.22919609805317975 1107 36-37 0.32933731535965904 1107 38-39 0.3327036406920101 1107 40-41 0.3248212960689969 1107 42-43 0.33460908618494045 1107 44-45 0.3083153372343048 1107 46-47 0.3680629457240059 1107 48-49 0.3554206387100436 1107 50-51 0.37381072443155716 1107 52-53 0.30748177612716177 1107 54-55 0.3035387450694529 1107 56-57 0.3064710189345732 1107 58-59 0.3045320211026521 1107 60-61 0.2576242040878327 1107 62-63 0.19656926783038386 1107 64-65 0.09983712608269713 1107 66-67 -10.250878325459084 1107 68-69 -8.191426849931819 1107 70-71 -6.7027572051383295 1107 72-73 -1.6855191321371912 1107 74-75 -0.47043625058621785 1107 76-77 -0.1863355308839516 1107 78-79 2.9597836590530555 1107 80-81 0.8896367892133945 1107 82-83 0.25096238163661866 1107 84-85 -5.464414058662694 1107 86-87 -5.967042474755864 1107 88-89 -1.4585870278897666 1107 90-91 -0.22233801264572506 1107 92-93 0.11672286099177498 1107 94-95 0.2178961835788762 1107 96-97 0.2464228367840775 1107 98-99 0.30671785861118295 1107 100 0.3896191316428528 1108 1 -0.08121663743356322 1108 2 -0.010353152724718484 1108 3 0.0059332516953247705 1108 4 -0.07526648102471967 1108 5 -0.07882730281002637 1108 6 -0.024516799985377702 1108 7 -12.298899170580857 1108 8 -6.771517398540389 1108 9 -2.6947693753976907 1108 10-11 -0.641062485295798 1108 12-13 -0.07575321965627069 1108 14-15 0.08827477324572897 1108 16-17 0.05737066850236516 1108 18-19 0.09309697232098557 1108 20-21 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-0.6833473670820638 2216 6 -0.9724639487905691 2216 7 3.133567433445336 2216 8 1.2105322867696913 2216 9 0.00727036972714501 2216 10-11 -0.6772383098434673 2216 12-13 -1.0618550241192253 2216 14-15 -1.2762200695410826 2216 16-17 -1.4091986391056253 2216 18-19 -1.9265633328031129 2216 20-21 -1.4808025826840208 2216 22-23 -1.3387523855183545 2216 24-25 -1.3185880270068466 2216 26-27 -1.8594972265531382 2216 28-29 -2.040000783554561 2216 30-31 -1.920461789229151 2216 32-33 -2.851927222826369 2216 34-35 -1.8729421352621642 2216 36-37 -1.545136983620587 2216 38-39 -1.7527810274721887 2216 40-41 -1.8153674253183993 2216 42-43 -1.435034648018842 2216 44-45 -1.6175992532926813 2216 46-47 -1.818787919755465 2216 48-49 -1.8168334163037372 2216 50-51 -2.4011527126201955 2216 52-53 -2.0902247191748557 2216 54-55 -1.6897374185568381 2216 56-57 -1.726585343580851 2216 58-59 -2.062227280313273 2216 60-61 -2.233554261737339 2216 62-63 -2.0413560471862837 2216 64-65 -1.7169568239902517 2216 66-67 1.6556887611322324 2216 68-69 0.824420677713988 2216 70-71 0.3905127324320432 2216 72-73 -1.367808838822853 2216 74-75 -2.1186134397124334 2216 76-77 -1.7807547947727222 2216 78-79 0.812641403234938 2216 80-81 -0.6062144500827849 2216 82-83 -1.5146822446513717 2216 84-85 0.2905851886539317 2216 86-87 0.22192181833775138 2216 88-89 -1.0277796097752514 2216 90-91 -1.2963675204800857 2216 92-93 -1.3495744832893948 2216 94-95 -1.3497327203387606 2216 96-97 -1.3060052999690726 2216 98-99 -1.3282702623117117 2216 100 -2.586963860807021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127222.0 3 26179.0 4 14875.0 5 13106.0 6 12039.0 7 13062.0 8 14446.0 9 16357.0 10 18584.0 11 20817.0 12 23701.0 13 26562.0 14 30092.0 15 33272.0 16 35581.0 17 36761.0 18 34589.0 19 32116.0 20 31363.0 21 31944.0 22 34260.0 23 39936.0 24 48415.0 25 61024.0 26 76088.0 27 92948.0 28 114016.0 29 144489.0 30 176586.0 31 214325.0 32 281875.0 33 393433.0 34 591794.0 35 813960.0 36 899488.0 37 1489017.0 38 1703930.0 39 588217.0 40 1662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79821386047146 20.71081795521368 14.730505458579298 22.760462725735557 2 15.212973225976889 20.4759850259968 40.71754659514675 23.593495152879555 3 18.425585612705177 25.545842620298227 30.414686886556314 25.613884880440285 4 12.657470896352502 17.087600067624052 38.754887100018145 31.500041936005296 5 13.032184733189759 38.262084230677196 35.255372098588175 13.450358937544868 6 30.34830727998229 37.86611389292701 18.051652039699203 13.733926787391496 7 26.94612618038239 33.04519879145084 22.43417771627515 17.574497311891623 8 24.764755935778922 36.538648367194185 20.648606960793135 18.04798873623376 9 24.888255520283185 17.523187899304283 21.957181575641734 35.6313750047708 10-11 23.653906597060995 27.560701070610165 28.8148510713699 19.97054126095894 12-13 23.965932096541678 25.155091491147964 28.93109117337357 21.94788523893679 14-15 21.458864427944476 27.372536994215572 26.248332312570838 24.920266265269113 16-17 20.894791129139648 30.67676688034743 27.055121452740433 21.37332053777249 18-19 20.91288710358811 29.660051990092047 28.861410523477076 20.56565038284277 20-21 22.13803540528379 28.715235499419666 28.395546803465987 20.751182291830553 22-23 21.826495769598235 28.568308427345084 28.476822657889823 21.128373145166854 24-25 21.491353748822554 28.754245416828233 28.413852331340582 21.340548503008627 26-27 21.192349277881263 28.849310843248947 28.470020685291047 21.488319193578743 28-29 21.180488795880322 28.87253741296948 28.660366773385103 21.286607017765096 30-31 21.529621873598295 29.08970318842813 28.21894631706538 21.16172862090819 32-33 21.22408234712296 28.94906342991414 28.57149693941725 21.255357283545646 34-35 21.452125925372016 28.6905256860839 28.50693187766395 21.350416510880134 36-37 21.595598727897116 28.801068995226085 28.249282823943012 21.354049452933786 38-39 21.407432466183366 28.9361223551567 28.42777284744699 21.22867233121294 40-41 21.353996485577937 28.736448375839046 28.508371070039463 21.401184068543554 42-43 21.293622363000704 28.760941297015467 28.509461509737765 21.43597483024606 44-45 21.42555340375471 28.83988621597775 28.362876775273087 21.371683604994452 46-47 21.48255704742472 28.933808213067984 28.256682243672742 21.326952495834554 48-49 21.38422250780114 28.761020335912434 28.32462179371293 21.530135362573493 50-51 21.348820676101514 28.755718708116945 28.234415645480897 21.661044970300644 52-53 21.353132709392323 28.803640201607816 28.296707323194 21.546519765805865 54-55 21.441869057258973 28.96619038594436 28.179007515587283 21.412933041209385 56-57 21.418268656987852 28.798729650445093 28.133912665502077 21.64908902706498 58-59 21.376900005342122 28.581239411844905 28.342901794145476 21.6989587886675 60-61 21.447275861113866 28.802050340472118 28.14096724866809 21.609706549745926 62-63 21.28503797350828 28.83192875941082 28.22338707709036 21.65964618999054 64-65 21.37838882954489 28.719983421966468 28.185408841873127 21.716218906615516 66-67 21.242001463253644 28.675199158905397 28.17468792188326 21.908111455957695 68-69 21.672197298352323 28.79405235130404 27.995256204799347 21.53849414554429 70-71 21.39384643179549 28.611424021421612 28.100949818226194 21.8937797285567 72-73 21.256895847156134 28.666522015173573 28.27760811039027 21.79897402728003 74-75 21.268811789290613 28.763466092311212 28.129310859002043 21.838411259396135 76-77 21.31785113830232 28.70216945721602 28.02084702464277 21.959132379838884 78-79 21.453507941204112 28.66603733895824 28.156878925840562 21.723575793997085 80-81 21.241178207624287 28.654038330107568 28.195760992960757 21.90902246930739 82-83 21.222341645830685 28.671307795399642 28.32301973608406 21.783330822685613 84-85 21.267740056382582 28.55360755889623 28.137942692925368 22.040709691795822 86-87 21.377332930996236 28.583853184928053 28.1120323758524 21.926781508223304 88-89 21.23781862237629 28.507422265882354 28.221618023512463 22.03314108822889 90-91 21.205572656254535 28.45372051924147 28.166056670944684 22.174650153559313 92-93 21.26987570200739 28.532895786914718 28.130500089855985 22.066728421221903 94-95 21.21952694697936 28.3801660813134 28.242467925219554 22.15783904648768 96-97 21.254619146613223 28.432568086983835 28.160896160806427 22.15191660559652 98-99 21.23466917581834 28.366469753778322 28.136118526785932 22.26274254361741 100 21.27919165823228 28.37264241528094 28.04944163152024 22.29872429496654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3074.0 1 2424.0 2 2066.5 3 2423.5 4 2348.0 5 2114.5 6 2002.5 7 1960.0 8 1975.5 9 2025.0 10 1924.0 11 1736.0 12 1711.0 13 1899.5 14 2082.0 15 2264.0 16 2664.5 17 3409.0 18 4580.0 19 6320.5 20 9078.5 21 13241.0 22 20200.5 23 30561.5 24 42168.0 25 55266.5 26 73248.5 27 95746.0 28 123129.5 29 148989.5 30 167814.0 31 187065.5 32 207820.5 33 226820.0 34 244436.5 35 261571.5 36 274447.5 37 283560.5 38 291416.0 39 295284.0 40 298299.0 41 296325.5 42 289206.0 43 286996.5 44 283025.5 45 277019.0 46 273188.0 47 266712.5 48 258958.5 49 253796.0 50 248153.5 51 237193.0 52 223597.0 53 211091.5 54 201771.0 55 191651.0 56 175253.5 57 156411.5 58 140019.0 59 125704.5 60 109270.5 61 91796.0 62 77071.5 63 63595.5 64 51479.0 65 41048.5 66 32290.5 67 25099.0 68 19086.0 69 14581.5 70 10874.0 71 7876.5 72 5780.0 73 4004.0 74 2756.0 75 1869.5 76 1177.5 77 761.5 78 506.5 79 330.0 80 228.5 81 172.0 82 130.0 83 93.0 84 75.5 85 66.5 86 54.5 87 46.5 88 35.5 89 30.0 90 25.0 91 20.5 92 23.0 93 19.5 94 16.0 95 16.5 96 17.5 97 16.5 98 14.0 99 18.5 100 33.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01867642419100634 2 0.0021416271173543464 3 0.001076795757329001 4 0.0018903747739775793 5 0.001567336046778879 6 0.003361995642327214 7 11.59476921335643 8 3.5893191910966695E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8714553528773356E-4 18-19 0.0 20-21 0.0 22-23 5.982198651827783E-6 24-25 0.0 26-27 2.1535915146580017E-4 28-29 0.0 30-31 0.0 32-33 5.982198651827783E-6 34-35 7.178638382193339E-5 36-37 2.392879460731113E-5 38-39 4.187539056279448E-5 40-41 0.0 42-43 4.785758921462226E-5 44-45 1.8544815820666126E-4 46-47 0.0010169737708107232 48-49 7.178638382193339E-4 50-51 4.4268270023525597E-4 52-53 2.6919893933225023E-4 54-55 4.187539056279448E-4 56-57 0.0013938522858758734 58-59 3.529497204578392E-4 60-61 0.0012502795182320068 62-63 0.0023809150634274577 64-65 9.93044976203412E-4 66-67 5.7977255920013695 68-69 5.4459065070887265 70-71 0.0025843098175896023 72-73 0.0013340302993575957 74-75 0.002877437551529164 76-77 0.005832643685532088 78-79 5.4119754763355585 80-81 0.005013082470231682 82-83 0.004713972537640293 84-85 5.797952915550139 86-87 0.003553425999185703 88-89 0.0016451046292526403 90-91 0.003415835430193664 92-93 0.0037448563560441924 94-95 0.004277272036056865 96-97 0.003062885709735825 98-99 0.0054916583623779045 100 0.00571898191114736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8358131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.59144615418592 #Duplication Level Percentage of deduplicated Percentage of total 1 90.38413665026015 77.36108965293325 2 6.8886838102155945 11.792248188305607 3 1.4672378878623316 3.767490380230507 4 0.5257408283443142 1.799956712011579 5 0.24404360497073913 1.044402253706322 6 0.14210150045237827 0.7297603754639257 7 0.09188537800779538 0.5505221668877865 8 0.06557556056078886 0.44901656486154545 9 0.04209131968540093 0.32423912301704577 >10 0.1449032018146902 1.9310025955073342 >50 0.0031307163761530943 0.18298161132843652 >100 4.5476926764356096E-4 0.060677707965130744 >500 1.4772182053076634E-5 0.006612667781577122 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.5893191910966695E-5 0.0 0.0 0.0 6 0.0 3.5893191910966695E-5 0.0 0.0 0.0 7 0.0 3.5893191910966695E-5 0.0 0.0 0.0 8 0.0 3.5893191910966695E-5 0.0 0.0 0.0 9 0.0 3.5893191910966695E-5 0.0 0.0 0.0 10-11 0.0 4.785758921462226E-5 0.0 2.9910993259138915E-5 0.0 12-13 0.0 9.571517842924452E-5 0.0 4.785758921462226E-5 0.0 14-15 0.0 1.1964397303655566E-4 0.0 4.785758921462226E-5 0.0 16-17 0.0 1.4955496629569457E-4 0.0 4.785758921462226E-5 0.0 18-19 0.0 1.5553716494752235E-4 0.0 7.178638382193339E-5 0.0 20-21 0.0 1.5553716494752235E-4 0.0 1.435727676438668E-4 0.0 22-23 0.0 1.5553716494752235E-4 0.0 2.6919893933225023E-4 0.0 24-25 0.0 1.5553716494752235E-4 0.0 5.443800773163283E-4 0.0 26-27 0.0 1.6750156225117793E-4 0.0 9.870627775515842E-4 0.0 28-29 0.0 1.6750156225117793E-4 0.0 0.003302173655808936 0.0 30-31 0.0 1.8544815820666126E-4 0.0 0.009643304226746387 0.0 32-33 0.0 2.0339475416214462E-4 0.0 0.023785221839667265 0.0 34-35 0.0 2.153591514658002E-4 0.0 0.04655346990852381 0.0 36-37 0.0 2.153591514658002E-4 0.0 0.08373283452963348 0.0 38-39 0.0 2.3330574742128356E-4 0.0 0.13343892312767053 0.0 40-41 0.0 2.512523433767669E-4 0.0 0.1837073383989794 0.0 42-43 0.0 2.7518113798407804E-4 0.0 0.23432870339074607 0.0 44-45 1.1964397303655566E-5 2.7518113798407804E-4 0.0 0.29980386763500116 0.0 46-47 1.1964397303655566E-5 2.7518113798407804E-4 0.0 0.37950470027330274 0.0 48-49 2.392879460731113E-5 2.7518113798407804E-4 0.0 0.4626811903283162 0.0 50-51 2.392879460731113E-5 2.7518113798407804E-4 0.0 0.5461268793226619 0.0 52-53 2.392879460731113E-5 2.7518113798407804E-4 0.0 0.6273890658090906 0.0 54-55 2.392879460731113E-5 2.9910993259138914E-4 0.0 0.7162366801860368 0.0 56-57 2.392879460731113E-5 2.9910993259138914E-4 0.0 0.8137046428202668 0.0 58-59 2.392879460731113E-5 2.9910993259138914E-4 0.0 0.9209594824488871 0.0 60-61 2.392879460731113E-5 2.9910993259138914E-4 0.0 1.0329881166016661 0.0 62-63 2.392879460731113E-5 2.9910993259138914E-4 0.0 1.1444424596838696 0.0 64-65 2.392879460731113E-5 2.9910993259138914E-4 0.0 1.2625549898655573 0.0 66-67 2.392879460731113E-5 2.9910993259138914E-4 0.0 1.3968134742085283 0.0 68-69 2.392879460731113E-5 2.9910993259138914E-4 0.0 1.5324598286387232 0.0 70-71 2.392879460731113E-5 3.110743298950447E-4 0.0 1.69478678905607 0.0 72-73 2.392879460731113E-5 3.4696752180601143E-4 0.0 1.8663801751850984 0.0 74-75 2.392879460731113E-5 3.4696752180601143E-4 0.0 2.021103761115972 0.0 76-77 2.392879460731113E-5 3.4696752180601143E-4 0.0 2.1845434104825587 0.0 78-79 2.392879460731113E-5 3.4696752180601143E-4 0.0 2.377475299202657 0.0 80-81 2.392879460731113E-5 3.4696752180601143E-4 0.0 2.609052191213562 0.0 82-83 2.392879460731113E-5 3.4696752180601143E-4 0.0 2.8490879121181516 0.0 84-85 2.392879460731113E-5 3.4696752180601143E-4 0.0 3.092126696745959 0.0 86-87 2.392879460731113E-5 3.58931919109667E-4 0.0 3.3732062826007394 0.0 88 2.392879460731113E-5 3.58931919109667E-4 0.0 3.6092279482099525 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8790 0.0 48.855637 1 GTATCAA 14850 0.0 38.810886 1 ATCAACG 16710 0.0 33.320744 3 TCAACGC 16705 0.0 33.177547 4 CAACGCA 18290 0.0 30.357946 5 TATCAAC 18740 0.0 30.339384 2 AACGCAG 20025 0.0 27.829153 6 GTACATG 28800 0.0 25.912342 1 TACATGG 28910 0.0 24.95935 2 GTGGTAT 4735 0.0 24.53847 1 ACGCAGA 22895 0.0 23.938145 7 ACATGGG 29915 0.0 23.36813 3 CGCAGAG 23065 0.0 23.320581 8 TGGTATC 5280 0.0 20.450825 2 GCAGAGT 25210 0.0 20.223766 9 CATGGGG 18095 0.0 19.372883 4 CATGGGT 8190 0.0 19.30798 4 GAGTACA 22040 0.0 19.089819 1 AGAGTAC 22275 0.0 17.445671 10-11 CAGAGTA 24270 0.0 17.176626 10-11 >>END_MODULE