FastQCFastQC Report
Wed 25 May 2016
SRR2049527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358131
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325490.389429167836685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310320.3712791771270395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183770.21986972924927833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.1080265432547061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182750.059.7969131
GTATCAA340000.041.972811
GTGGTAT68150.038.8958741
TGGTATC71650.035.726142
ATCAACG435000.032.5164533
TCAACGC440000.032.189724
TATCAAC444550.031.796752
CAACGCA458650.030.9668165
AACGCAG480650.029.6271176
ACGCAGA548900.025.720377
CGCAGAG546450.025.6292748
GTACATG308900.024.5396861
GCAGAGT581100.023.6966659
TACATGG309600.023.572972
ACATGGG319900.022.181483
GAGTACT403950.021.75627112-13
CAGAGTA571500.020.97391910-11
AGAGTAC546700.020.79922110-11
GTACTTT433300.020.11989614-15
CATGGGT92150.020.067844
Produced by FastQC (version 0.11.5)