##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049527_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8358131
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.842500554250705	33.0	31.0	34.0	30.0	34.0
2	31.926017072477087	33.0	31.0	34.0	30.0	34.0
3	31.948216772386075	33.0	31.0	34.0	30.0	34.0
4	35.605460359499034	37.0	35.0	37.0	33.0	37.0
5	35.72922104235983	37.0	35.0	37.0	33.0	37.0
6	35.80121070129195	37.0	35.0	37.0	35.0	37.0
7	35.78746420700992	37.0	35.0	37.0	33.0	37.0
8	35.76615465826032	37.0	35.0	37.0	33.0	37.0
9	37.47397091526802	39.0	37.0	39.0	34.0	39.0
10-11	37.446907927143045	39.0	37.0	39.0	34.5	39.0
12-13	37.43704046993281	39.0	37.0	39.0	34.0	39.0
14-15	38.69276743807916	40.0	38.0	41.0	34.0	41.0
16-17	38.70065634290728	40.0	38.0	41.0	34.0	41.0
18-19	38.79320669896177	40.0	38.0	41.0	35.0	41.0
20-21	38.83440239211374	40.0	38.0	41.0	35.0	41.0
22-23	38.80852459718567	40.0	38.0	41.0	35.0	41.0
24-25	38.77375887025461	40.0	38.0	41.0	35.0	41.0
26-27	38.680978139730044	40.0	38.0	41.0	34.0	41.0
28-29	38.594052426313965	40.0	38.0	41.0	34.0	41.0
30-31	38.46812188035818	40.0	38.0	41.0	34.0	41.0
32-33	38.31031411209037	40.0	38.0	41.0	34.0	41.0
34-35	38.2077345999961	40.0	38.0	41.0	34.0	41.0
36-37	38.07237886077641	40.0	38.0	41.0	33.5	41.0
38-39	37.981718281276045	40.0	37.0	41.0	33.0	41.0
40-41	37.853146833903416	40.0	37.0	41.0	33.0	41.0
42-43	37.69817038043553	40.0	37.0	41.0	33.0	41.0
44-45	37.48879683747479	40.0	37.0	41.0	33.0	41.0
46-47	37.20197775076748	40.0	37.0	41.0	32.0	41.0
48-49	36.93374840619272	40.0	37.0	41.0	31.0	41.0
50-51	36.71041863306522	40.0	36.0	41.0	31.0	41.0
52-53	36.60856099288226	40.0	36.0	41.0	31.0	41.0
54-55	36.479405742743204	39.5	36.0	41.0	30.5	41.0
56-57	36.307869725899245	39.0	35.0	41.0	30.0	41.0
58-59	36.132574435600496	39.0	35.0	41.0	30.0	41.0
60-61	35.94519241203566	39.0	35.0	41.0	30.0	41.0
62-63	35.75388714295097	39.0	35.0	41.0	29.0	41.0
64-65	35.484493423230624	38.0	35.0	40.0	29.0	41.0
66-67	35.233279665035155	37.5	35.0	40.0	29.0	41.0
68-69	34.93123492560717	37.0	34.5	40.0	28.5	41.0
70-71	34.645225050911506	36.5	34.0	39.5	28.0	41.0
72-73	34.330526824717154	36.0	34.0	39.0	28.0	41.0
74-75	34.00123598206345	36.0	34.0	39.0	28.0	40.5
76-77	33.70973014182238	35.0	34.0	38.5	28.0	40.0
78-79	33.3862764295032	35.0	34.0	37.0	27.0	39.0
80-81	33.08089368304948	35.0	34.0	37.0	27.0	39.0
82-83	32.789128155564924	35.0	33.5	36.0	26.0	39.0
84-85	32.51313008853295	35.0	33.0	36.0	26.0	37.0
86-87	32.23854782845591	35.0	33.0	36.0	26.0	37.0
88-89	32.02993462294381	35.0	33.0	35.0	26.0	37.0
90-91	31.879634035408156	35.0	33.0	35.0	26.0	36.0
92-93	31.724026101050583	35.0	33.0	35.0	25.5	36.0
94-95	31.543874820818196	35.0	33.0	35.0	25.0	36.0
96-97	31.380046089251294	35.0	33.0	35.0	25.0	36.0
98-99	31.199265840652654	35.0	33.0	35.0	24.0	35.0
100	31.086357823298055	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3900461731629896
1101	2	0.4379424438762456
1101	3	0.4817090191345663
1101	4	0.3960972190820158
1101	5	0.32657600184725055
1101	6	0.32810029394130424
1101	7	0.3246555298800402
1101	8	0.33922701257994703
1101	9	0.3543205151119082
1101	10-11	0.35357811231231295
1101	12-13	0.3483586454382319
1101	14-15	0.513477315517143
1101	16-17	0.48644129331963626
1101	18-19	0.45507930845088396
1101	20-21	0.4537163868650609
1101	22-23	0.49090343717620044
1101	24-25	0.5255808456526339
1101	26-27	0.49354074094878797
1101	28-29	0.47883612395831676
1101	30-31	0.4992708228485867
1101	32-33	0.5260702256921803
1101	34-35	0.5694075607428175
1101	36-37	0.590347615428719
1101	38-39	0.5778377460348523
1101	40-41	0.6273055618135999
1101	42-43	0.5162835828820462
1101	44-45	0.5012456151792364
1101	46-47	0.558988296055638
1101	48-49	0.5836960552962083
1101	50-51	0.6387529031523584
1101	52-53	0.6144127242539383
1101	54-55	0.5566060311016585
1101	56-57	0.538718741115666
1101	58-59	0.5531624824541836
1101	60-61	0.5964436148815437
1101	62-63	0.5905451421650696
1101	64-65	0.6524436335844825
1101	66-67	0.6763377951561438
1101	68-69	0.5992257305795121
1101	70-71	0.626965025296677
1101	72-73	0.600379347083603
1101	74-75	0.6353570583540957
1101	76-77	0.6226763746639747
1101	78-79	0.5784745389695516
1101	80-81	0.565315965306155
1101	82-83	0.5820550210943551
1101	84-85	0.5514744496149078
1101	86-87	0.624665238088042
1101	88-89	0.6206076492549002
1101	90-91	0.6019954620866486
1101	92-93	0.6281365358915068
1101	94-95	0.5399341039895553
1101	96-97	0.5775660829708222
1101	98-99	0.6471915357200011
1101	100	0.6859218503669346
1102	1	0.2520156442812329
1102	2	0.3138205559915619
1102	3	0.38823052002726044
1102	4	0.35408413110627635
1102	5	0.285839418335712
1102	6	0.25504615811032494
1102	7	0.25269714173379043
1102	8	0.2790441690789507
1102	9	0.33600073452411294
1102	10-11	0.3122133307723516
1102	12-13	0.2971992892282955
1102	14-15	0.38683752815844485
1102	16-17	0.3911810331798833
1102	18-19	0.35786655551797963
1102	20-21	0.33500664472109776
1102	22-23	0.3451952031627883
1102	24-25	0.3232450308717816
1102	26-27	0.2978915115400156
1102	28-29	0.32827969163923143
1102	30-31	0.39239641794727476
1102	32-33	0.4163833725484878
1102	34-35	0.43674345943029635
1102	36-37	0.47014087677745664
1102	38-39	0.45692899340546944
1102	40-41	0.45649164556993327
1102	42-43	0.47655919793591295
1102	44-45	0.5127846744472393
1102	46-47	0.5419447864527029
1102	48-49	0.6131635181273865
1102	50-51	0.6096608094191609
1102	52-53	0.5695077904708654
1102	54-55	0.5226247707364493
1102	56-57	0.5525106351510729
1102	58-59	0.5113889953455271
1102	60-61	0.48872516031042323
1102	62-63	0.4652519395509671
1102	64-65	0.5127329071792133
1102	66-67	0.5491860970682652
1102	68-69	0.5381427804806123
1102	70-71	0.5179476157644416
1102	72-73	0.5655679874386053
1102	74-75	0.5671013678426178
1102	76-77	0.5556683904004842
1102	78-79	0.5171488506250554
1102	80-81	0.49621411651466474
1102	82-83	0.5171437979618076
1102	84-85	0.5277527307108869
1102	86-87	0.5672328321783908
1102	88-89	0.5181268802907297
1102	90-91	0.49644648286557924
1102	92-93	0.49719354935523796
1102	94-95	0.5284380049184314
1102	96-97	0.504692317219174
1102	98-99	0.5175180492680056
1102	100	0.5540133830201164
1103	1	0.2675347970900397
1103	2	0.3017762108273345
1103	3	0.37071087081736565
1103	4	0.3195627444925577
1103	5	0.22195756179562665
1103	6	0.24849862770576436
1103	7	0.24605860446234118
1103	8	0.27345303811179633
1103	9	0.32473408440414886
1103	10-11	0.25067117804628936
1103	12-13	0.26194736567406807
1103	14-15	0.37290024016308365
1103	16-17	0.35762431935511074
1103	18-19	0.35632026611614265
1103	20-21	0.34715688878497275
1103	22-23	0.3498332440331424
1103	24-25	0.3919097119340833
1103	26-27	0.38594230991844825
1103	28-29	0.3587181792705749
1103	30-31	0.38409162767673877
1103	32-33	0.3865488395791772
1103	34-35	0.4218547360084415
1103	36-37	0.48525132602043897
1103	38-39	0.483685839603865
1103	40-41	0.46093443534407186
1103	42-43	0.4227393915323461
1103	44-45	0.4496217721555169
1103	46-47	0.39265050333860785
1103	48-49	0.38297819505301334
1103	50-51	0.3744212958922333
1103	52-53	0.3692761366278958
1103	54-55	0.3726338714231403
1103	56-57	0.40491900778282996
1103	58-59	0.36266805549279013
1103	60-61	0.36068511728899466
1103	62-63	0.4173128314182719
1103	64-65	0.39949885406429786
1103	66-67	0.4397263469234787
1103	68-69	0.43798889614206615
1103	70-71	0.4521082842512456
1103	72-73	0.43068588269797203
1103	74-75	0.3992921513290142
1103	76-77	0.3975563692207089
1103	78-79	0.34017110594067645
1103	80-81	0.30779101731718583
1103	82-83	0.3445972603594214
1103	84-85	0.38349443667597427
1103	86-87	0.3809938977860696
1103	88-89	0.4135922563145513
1103	90-91	0.3505161444854963
1103	92-93	0.31398183428946425
1103	94-95	0.381365944026566
1103	96-97	0.38735468174304444
1103	98-99	0.41208162997455133
1103	100	0.41458589894631714
1104	1	0.22384423389897634
1104	2	0.23364023017972357
1104	3	0.3153469310710939
1104	4	0.24857918498485532
1104	5	0.1789883777406942
1104	6	0.2006507070445025
1104	7	0.17505233865722403
1104	8	0.1803437102126395
1104	9	0.2047828224291024
1104	10-11	0.24850173445710055
1104	12-13	0.23979853902751103
1104	14-15	0.31658719796684665
1104	16-17	0.30456788781044253
1104	18-19	0.2894640571736389
1104	20-21	0.3013948871307903
1104	22-23	0.29587107792691825
1104	24-25	0.29715568921383095
1104	26-27	0.2862771336148455
1104	28-29	0.29242336626913357
1104	30-31	0.32696477555734305
1104	32-33	0.2884594682495134
1104	34-35	0.29697134377314427
1104	36-37	0.3286121042080907
1104	38-39	0.3560280857589504
1104	40-41	0.29970002407702623
1104	42-43	0.30975019073990495
1104	44-45	0.3559552215276156
1104	46-47	0.3713153692960063
1104	48-49	0.335845740639094
1104	50-51	0.40468099700697024
1104	52-53	0.4000843017534521
1104	54-55	0.35980072396702667
1104	56-57	0.3826666620067769
1104	58-59	0.38530087085774056
1104	60-61	0.3983706458228511
1104	62-63	0.34203908896921575
1104	64-65	0.3354600863497339
1104	66-67	0.3339833544693249
1104	68-69	0.28512029736457123
1104	70-71	0.2867388597978291
1104	72-73	0.3705463433494174
1104	74-75	0.3649091588941644
1104	76-77	0.3388958196721319
1104	78-79	0.3226596466851177
1104	80-81	0.2793110521520106
1104	82-83	0.2876021309104999
1104	84-85	0.34053771862637205
1104	86-87	0.38639062222132736
1104	88-89	0.3178314737746959
1104	90-91	0.3306008043154307
1104	92-93	0.3308569272691386
1104	94-95	0.29664843996445356
1104	96-97	0.2819335394905167
1104	98-99	0.37250716140733076
1104	100	0.41557133348333863
1105	1	0.24435449180893087
1105	2	0.2621514223517707
1105	3	0.32792461535965245
1105	4	0.2652717423246713
1105	5	0.23857964923904973
1105	6	0.24985287402292045
1105	7	0.2509756254188602
1105	8	0.25937386492137904
1105	9	0.27257043595188435
1105	10-11	0.25277275409192157
1105	12-13	0.28080509531719855
1105	14-15	0.3347261833223669
1105	16-17	0.32010428041802186
1105	18-19	0.3277820470736543
1105	20-21	0.3296336407228324
1105	22-23	0.33534096001912417
1105	24-25	0.32234691569269813
1105	26-27	0.3144644499304974
1105	28-29	0.36640627191340513
1105	30-31	0.362050383627448
1105	32-33	0.369929814277981
1105	34-35	0.33732886530499684
1105	36-37	0.3343851462009795
1105	38-39	0.34436393596692483
1105	40-41	0.3392805912587704
1105	42-43	0.3252157849211841
1105	44-45	0.3583643663645475
1105	46-47	0.3862291354206704
1105	48-49	0.3769297960578868
1105	50-51	0.4311660204614469
1105	52-53	0.3726691572374463
1105	54-55	0.372007245773581
1105	56-57	0.4344912287412228
1105	58-59	0.4156290857800471
1105	60-61	0.42384335955700436
1105	62-63	0.37598638267145645
1105	64-65	0.3651132239071586
1105	66-67	0.4183472493735394
1105	68-69	0.42122132910537147
1105	70-71	0.4686077189797899
1105	72-73	0.42747698034902015
1105	74-75	0.4448149597788742
1105	76-77	0.3948506046767264
1105	78-79	0.31614097267959096
1105	80-81	0.31439850780699885
1105	82-83	0.35932978334970755
1105	84-85	0.36774917973941257
1105	86-87	0.428261347848931
1105	88-89	0.373171644912766
1105	90-91	0.36436916455389223
1105	92-93	0.3641408930916157
1105	94-95	0.36167364143925695
1105	96-97	0.40677968715190005
1105	98-99	0.4479899242711376
1105	100	0.47673948018845635
1106	1	0.15141500712367062
1106	2	0.1869580563922213
1106	3	0.29504285858586243
1106	4	0.2347431705190388
1106	5	0.16336067647242203
1106	6	0.18676909733583358
1106	7	0.17965250204239425
1106	8	0.18625846345553043
1106	9	0.17076953277065599
1106	10-11	0.22479950912558877
1106	12-13	0.18511605957102972
1106	14-15	0.25364666804198777
1106	16-17	0.2664248082376872
1106	18-19	0.25401928630419945
1106	20-21	0.19514419435756025
1106	22-23	0.22142403796279098
1106	24-25	0.24516433173658214
1106	26-27	0.2536330275335601
1106	28-29	0.24868317079315716
1106	30-31	0.20672102332561337
1106	32-33	0.2358966154928197
1106	34-35	0.23327708038003436
1106	36-37	0.2787846550735864
1106	38-39	0.2866728350472698
1106	40-41	0.31740109365640023
1106	42-43	0.29601435254460284
1106	44-45	0.277077497948774
1106	46-47	0.26773229331349313
1106	48-49	0.2932871726929491
1106	50-51	0.32776568696905173
1106	52-53	0.3315284146552955
1106	54-55	0.2569261130234324
1106	56-57	0.2541126294324485
1106	58-59	0.25793837175258716
1106	60-61	0.3306482315143171
1106	62-63	0.340969250694485
1106	64-65	0.3256153595055693
1106	66-67	0.33260068269609633
1106	68-69	0.35111078414719543
1106	70-71	0.3172260634024937
1106	72-73	0.3590535023641621
1106	74-75	0.3685889202713142
1106	76-77	0.3222718766258623
1106	78-79	0.2999669197025554
1106	80-81	0.20883383799862543
1106	82-83	0.25580598846603664
1106	84-85	0.25987415281273485
1106	86-87	0.36212495454886096
1106	88-89	0.36924038930666114
1106	90-91	0.38254693778458204
1106	92-93	0.4168084592785064
1106	94-95	0.4140514879946977
1106	96-97	0.44052337959234933
1106	98-99	0.44314049722592586
1106	100	0.45562770938344244
1107	1	0.1897671385671309
1107	2	0.16559699147186535
1107	3	0.26569784509507244
1107	4	0.2088952186441162
1107	5	0.1894971344342551
1107	6	0.18704587405837714
1107	7	0.17173851852577116
1107	8	0.22032824024699948
1107	9	0.14914291262272883
1107	10-11	0.14805228895319544
1107	12-13	0.1669454210564183
1107	14-15	0.1883217386813314
1107	16-17	0.19849712990217938
1107	18-19	0.2395492864299058
1107	20-21	0.2711511737404919
1107	22-23	0.2060726761167757
1107	24-25	0.21607009689268608
1107	26-27	0.24008541217591528
1107	28-29	0.2785516192607105
1107	30-31	0.2513989460747723
1107	32-33	0.2428861588309985
1107	34-35	0.24461004361765504
1107	36-37	0.31395902359256667
1107	38-39	0.297345132768136
1107	40-41	0.23830899821228257
1107	42-43	0.2667322513738384
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2216	84-85	-1.2472414769475577
2216	86-87	-1.418626903616989
2216	88-89	-1.2428646855061203
2216	90-91	-1.0893697665217275
2216	92-93	-1.1514218548578938
2216	94-95	-1.2547210597037903
2216	96-97	-1.2930450787840613
2216	98-99	-1.2860247499052804
2216	100	-1.309892399813691
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	539.0
3	81.0
4	100.0
5	187.0
6	527.0
7	1475.0
8	3150.0
9	5200.0
10	7273.0
11	9995.0
12	14614.0
13	20721.0
14	25066.0
15	33281.0
16	44432.0
17	63844.0
18	65388.0
19	34019.0
20	23940.0
21	22799.0
22	23253.0
23	25131.0
24	28999.0
25	35342.0
26	45511.0
27	58329.0
28	77047.0
29	100776.0
30	131600.0
31	170179.0
32	219865.0
33	285488.0
34	387503.0
35	562059.0
36	917163.0
37	1666444.0
38	2308513.0
39	934820.0
40	3478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53932127336574	20.7591317388388	15.180204570678955	22.521342417116504
2	15.464684975581811	20.881146153306833	40.10286291859623	23.551305952515126
3	18.0649858281697	25.86354842530566	31.07135208473666	25.00011366178798
4	12.565591657262276	17.70662397726128	38.077989700829576	31.649794664646873
5	12.482177283045623	38.05755028138865	35.324723711245724	14.135548724320001
6	29.79522842556996	38.448618544445345	17.41811075286468	14.338042277120017
7	25.946003957104764	33.79340429098324	22.296001342883955	17.964590409028048
8	25.002024974243643	36.086476749407254	20.158430156215545	18.753068120133555
9	24.94390187844807	16.774962512516268	21.98833398359895	36.29280162543672
10-11	23.67281034480077	27.4438148911521	28.35175112713596	20.53162363691117
12-13	24.566972516579668	25.051656291538897	29.000948776819698	21.38042241506174
14-15	21.681063032296226	26.82956039917072	27.251891811180684	24.237484757352366
16-17	20.198062222283905	30.17723101013851	28.065395242070267	21.55931152550732
18-19	20.11118873346206	28.123177298848272	31.44549301751791	20.320140950171755
20-21	21.265729144470217	27.171493244123596	31.410688585761577	20.15208902564461
22-23	20.946776258950713	27.045879036832517	31.545192340249272	20.462152363967494
24-25	20.673096652828246	27.219901195614188	31.457517236808087	20.649484914749483
26-27	20.396814790292233	27.373882988912236	31.488104218514877	20.741198002280655
28-29	20.438030479831763	27.330665507553913	31.674670263649098	20.55663374896523
30-31	20.715449041544392	27.538530702075796	31.26332127218991	20.482698984189902
32-33	20.498974022958844	27.40864718491773	31.580549023492754	20.511829768630676
34-35	20.70757206365873	27.233175694422595	31.396014252468646	20.663237989450035
36-37	20.87237565431793	27.3269705870846	31.097257269597712	20.703396488999754
38-39	20.726415989411986	27.449055297171103	31.207126330037184	20.61740238337973
40-41	20.676171502935286	27.324374312869708	31.176312024781616	20.82314215941339
42-43	20.731112015353673	27.41644633231999	30.97628524846045	20.876156403865888
44-45	20.898146966110005	27.52623164197833	30.695869686656025	20.879751705255636
46-47	21.090779744897514	27.729213624433502	30.210521945635932	20.969484685033056
48-49	21.16270371928844	27.773368232682643	29.782782777632942	21.281145270395978
50-51	21.203568118279076	27.926315105613924	29.451195488560778	21.418921287546222
52-53	21.188875838390185	28.06604730172332	29.431932808901895	21.313144050984604
54-55	21.263001261884984	28.293783622199747	29.253794897447765	21.189420218467504
56-57	21.186620549498446	28.050978143319362	29.29153658874215	21.470864718440044
58-59	21.145767983915512	27.793212213113378	29.554686047911883	21.50633375505923
60-61	21.21834414894909	27.953934916789414	29.410618235105435	21.417102699156068
62-63	21.007238340724737	27.92986254941446	29.568907211432794	21.49399189842801
64-65	21.045608162877564	27.8558806986873	29.576702016276123	21.52180912215901
66-67	20.970764875544546	27.838777592741724	29.51416411156992	21.67629342014381
68-69	21.30304609966032	27.985544854465672	29.278070659576883	21.433338386297127
70-71	21.19478026845954	27.841577261710782	29.233951944519653	21.729690525310026
72-73	21.100022241814585	27.92799610343509	29.328979170103935	21.643002484646388
74-75	21.22898049815204	28.041562162641387	29.043071949937133	21.686385389269443
76-77	21.25651057634775	27.981034276682195	28.985002747623838	21.777452399346217
78-79	21.285714157524108	28.02072954825055	29.094841033282915	21.59871526094243
80-81	21.17200447756837	28.01483302201886	29.10663513800013	21.706527362412643
82-83	21.106204830913597	28.020562307847523	29.212440829166077	21.660792032072806
84-85	21.117107401164205	27.89473507893092	29.026124381156503	21.962033138748364
86-87	21.205179866788384	27.994354000236893	28.953300558857066	21.847165574117653
88-89	21.105505452929172	27.864140541132294	29.051368892954127	21.978985112984407
90-91	20.99598199260767	27.832397297814353	29.020648535562348	22.150972174015628
92-93	21.026316987494916	27.95848281193272	28.974278244867346	22.04092195570502
94-95	20.907237394438933	27.76683560105991	29.09857734021833	22.227349664282826
96-97	20.944443201476503	27.886342054222407	28.96127136557204	22.20794337872905
98-99	20.890892341829517	27.81280922379067	28.949511466416723	22.34678696796309
100	20.938446358213486	27.819197042555615	28.89162717469087	22.350729424540024
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1382.0
1	1055.5
2	1235.5
3	1870.5
4	1358.5
5	939.0
6	2379.0
7	6611.5
8	11223.5
9	12860.5
10	10239.0
11	6955.0
12	6642.0
13	7485.0
14	8444.5
15	9296.0
16	10264.5
17	11375.0
18	12755.0
19	14610.5
20	17060.0
21	20645.5
22	26591.5
23	36040.5
24	46635.0
25	57811.5
26	73998.0
27	94651.0
28	120485.0
29	145812.5
30	163342.0
31	181682.0
32	201834.0
33	219522.0
34	237040.5
35	254401.0
36	266822.0
37	275788.5
38	284581.5
39	288995.0
40	291837.0
41	290576.5
42	283872.0
43	282398.0
44	280072.0
45	274194.0
46	269560.5
47	263163.5
48	254733.5
49	250280.5
50	247376.0
51	236172.0
52	220884.5
53	208226.0
54	199156.0
55	189031.5
56	173291.5
57	154834.0
58	139742.5
59	127637.0
60	111878.5
61	93663.0
62	78405.5
63	65080.0
64	52408.0
65	41711.5
66	32988.0
67	25319.0
68	19100.5
69	14516.5
70	10665.5
71	7584.0
72	5456.5
73	3735.5
74	2492.5
75	1638.5
76	979.5
77	587.0
78	371.5
79	214.0
80	111.5
81	55.5
82	32.5
83	20.0
84	12.5
85	8.0
86	5.5
87	4.5
88	3.5
89	1.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	1.0
97	1.5
98	1.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05898447870702194
2	2.0339475416214462E-4
3	1.1964397303655566E-5
4	0.1206011248208481
5	0.0014117988818313568
6	0.0016151936359935016
7	0.0
8	0.0
9	9.571517842924452E-5
10-11	0.0
12-13	1.1964397303655566E-5
14-15	3.5893191910966695E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.6750156225117793E-4
30-31	2.811633366359058E-4
32-33	5.982198651827783E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.9741255551031686E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.6151936359935014E-4
80-81	2.75181137984078E-4
82-83	2.6919893933225023E-4
84-85	0.0
86-87	1.1964397303655566E-5
88-89	1.0169737708107231E-4
90-91	9.571517842924452E-5
92-93	6.042020638346061E-4
94-95	6.460774543974006E-4
96-97	0.0
98-99	6.580418517010562E-5
100	0.0012442973195801788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8358131.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7108370654543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18067522548532	36.99282136954122
2	16.788513068614233	17.6987315386195
3	5.8604255015212745	9.267238012347635
4	2.590176614695296	5.46121510031815
5	1.3469804685261317	3.5500234003415114
6	0.7894684794745368	2.496812663393656
7	0.5237692815944793	1.9325822083411652
8	0.3586231998134559	1.5122663242607148
9	0.24639524382761932	1.1688929595990474
>10	1.209297357950393	11.106899300017664
>50	0.06646024295044257	2.4105002696317523
>100	0.0358825399621073	3.610642208626011
>500	0.0022674303802752505	0.824173297011164
>1k	9.746775448384984E-4	0.8800958944690006
>5k	2.266691497517337E-5	0.10787777528094779
>10k+	6.80007449255201E-5	0.9792276782008992
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32549	0.389429167836685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31032	0.3712791771270395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18377	0.21986972924927833	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9029	0.1080265432547061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.1964397303655566E-5	0.0	0.0	0.0
5	0.0	2.392879460731113E-5	0.0	0.0	0.0
6	0.0	2.392879460731113E-5	0.0	0.0	0.0
7	0.0	2.392879460731113E-5	0.0	0.0	0.0
8	0.0	2.392879460731113E-5	0.0	0.0	0.0
9	0.0	2.392879460731113E-5	0.0	1.1964397303655566E-5	0.0
10-11	0.0	2.9910993259138915E-5	0.0	2.392879460731113E-5	0.0
12-13	0.0	5.383978786645004E-5	0.0	3.5893191910966695E-5	0.0
14-15	0.0	7.178638382193339E-5	0.0	3.5893191910966695E-5	0.0
16-17	5.982198651827783E-6	8.973297977741675E-5	0.0	3.5893191910966695E-5	0.0
18-19	1.1964397303655566E-5	9.571517842924452E-5	0.0	5.383978786645004E-5	0.0
20-21	1.1964397303655566E-5	9.571517842924452E-5	0.0	1.0767957573290009E-4	0.0
22-23	1.1964397303655566E-5	9.571517842924452E-5	0.0	2.2134135011762799E-4	0.0
24-25	1.1964397303655566E-5	1.1366177438472787E-4	0.0	4.606292961907393E-4	0.0
26-27	1.1964397303655566E-5	1.3759056899203902E-4	0.0	8.973297977741675E-4	0.0
28-29	1.1964397303655566E-5	1.4357276764386678E-4	0.0	0.0032244050733351752	0.0
30-31	1.1964397303655566E-5	1.4357276764386678E-4	0.0	0.009721072809220146	0.0
32-33	1.1964397303655566E-5	1.5553716494752235E-4	0.0	0.02398263439517758	0.0
34-35	1.1964397303655566E-5	1.7348376090300572E-4	0.0	0.04700213480741089	0.0
36-37	1.1964397303655566E-5	1.8544815820666126E-4	0.0	0.08463016432740765	0.0
38-39	1.1964397303655566E-5	1.9143035685848905E-4	0.0	0.13495241938658298	0.0
40-41	1.1964397303655566E-5	1.9143035685848905E-4	0.0	0.1857113749473417	0.0
42-43	1.1964397303655566E-5	2.0339475416214462E-4	0.0	0.23676944044069181	0.0
44-45	1.1964397303655566E-5	2.0339475416214462E-4	0.0	0.3026753229878785	0.0
46-47	1.1964397303655566E-5	2.0339475416214462E-4	0.0	0.3827829451345044	0.0
48-49	1.1964397303655566E-5	2.0339475416214462E-4	0.0	0.4664140822870568	0.0
50-51	2.392879460731113E-5	2.153591514658002E-4	0.0	0.5500631660355646	0.0
52-53	2.392879460731113E-5	2.153591514658002E-4	0.0	0.6318039284141395	0.0
54-55	2.392879460731113E-5	2.153591514658002E-4	0.0	0.7244681855309518	0.0
56-57	2.392879460731113E-5	2.153591514658002E-4	0.0	0.8360840479767546	0.0
58-59	2.392879460731113E-5	2.153591514658002E-4	0.0	0.9589643904839491	0.0
60-61	2.392879460731113E-5	2.153591514658002E-4	0.0	1.0874799641211652	0.0
62-63	2.392879460731113E-5	2.153591514658002E-4	0.0	1.215738303216353	0.0
64-65	2.392879460731113E-5	2.153591514658002E-4	0.0	1.3520367172995973	0.0
66-67	2.392879460731113E-5	2.153591514658002E-4	0.0	1.5114443647748521	0.0
68-69	2.392879460731113E-5	2.153591514658002E-4	0.0	1.6899830835386522	0.0
70-71	2.392879460731113E-5	2.153591514658002E-4	0.0	1.8818441587000732	0.0
72-73	2.392879460731113E-5	2.3928794607311132E-4	0.0	2.078903764489932	0.0
74-75	2.392879460731113E-5	2.3928794607311132E-4	0.0	2.2773033827778004	0.0
76-77	2.392879460731113E-5	2.3928794607311132E-4	0.0	2.494744339374437	0.0
78-79	2.392879460731113E-5	2.3928794607311132E-4	0.0	2.7413186034054746	0.0
80-81	2.392879460731113E-5	2.3928794607311132E-4	0.0	3.007023938725057	0.0
82-83	2.392879460731113E-5	2.512523433767669E-4	0.0	3.283024637924435	0.0
84-85	2.392879460731113E-5	2.6321674068042247E-4	0.0	3.5602277590528315	0.0
86-87	2.392879460731113E-5	2.6321674068042247E-4	0.0	3.85275727312721	0.0
88	2.392879460731113E-5	2.6321674068042247E-4	0.0	4.091704233877167	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18275	0.0	59.796913	1
GTATCAA	34000	0.0	41.97281	1
GTGGTAT	6815	0.0	38.895874	1
TGGTATC	7165	0.0	35.72614	2
ATCAACG	43500	0.0	32.516453	3
TCAACGC	44000	0.0	32.18972	4
TATCAAC	44455	0.0	31.79675	2
CAACGCA	45865	0.0	30.966816	5
AACGCAG	48065	0.0	29.627117	6
ACGCAGA	54890	0.0	25.72037	7
CGCAGAG	54645	0.0	25.629274	8
GTACATG	30890	0.0	24.539686	1
GCAGAGT	58110	0.0	23.696665	9
TACATGG	30960	0.0	23.57297	2
ACATGGG	31990	0.0	22.18148	3
GAGTACT	40395	0.0	21.756271	12-13
CAGAGTA	57150	0.0	20.973919	10-11
AGAGTAC	54670	0.0	20.799221	10-11
GTACTTT	43330	0.0	20.119896	14-15
CATGGGT	9215	0.0	20.06784	4
>>END_MODULE