FastQCFastQC Report
Wed 25 May 2016
SRR2049526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8247866
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.044.606441
GTATCAA130100.035.914271
TCAACGC137550.032.6608164
ATCAACG138900.032.4170533
CAACGCA152950.029.4726775
TATCAAC160800.028.4793532
AACGCAG168300.026.814256
GTACATG237950.023.6581341
ACGCAGA192150.023.1395367
CGCAGAG196400.022.5065368
TACATGG241250.022.2273182
ACATGGG249550.020.790913
GTCGTAC4600.018.9131831
GCAGAGT227250.018.6150139
GTGGTAT48850.018.019281
CATGGGG173250.017.6612364
CAGAGTA214800.016.6820410-11
AGAGTAC195600.016.38812410-11
GAGTACA181100.016.3336371
TGGTATC56400.014.9691792
Produced by FastQC (version 0.11.5)